Density of bacteria on Zostera marina, activity strength of Zostera marina surface extract against bacterial settlers and Rosmarinic acid concentration in Zostera marina leaf surface extracts, measured in May, July and August after three different heatwave regimes simulated in outdoor mesocosms at Kiel/Germany. To investigate the consequence of heatwaves on the chemical defense of eelgrass against microbial colonizers Z. marina plants were incubated in the Kiel Outdoor Benthocosm system under ambient control conditions and two different heatwave treatments: a treatment experiencing two spring heatwaves followed by a summer heatwave, and a treatment only experiencing just the summer heatwave. The capacity to deter microbial colonizers was found to be significantly up-regulated in Z. marina from both heatwave treatments in comparison to Z. marina under control conditions, suggesting defense regulation of Z. marina in response to marine heatwaves.
|#||Name||Short Name||Unit||Principal Investigator||Method/Device||Comment|
|1||Treatment||Treat||Guan, Chi||Heat wave treatment in tank|
|3||Bacteria||Bact||Guan, Chi||Fluorescence after DNA staining||Density of epiphytic bacteria on Zostera leaves; log(RU), RU = Fluorescence of stained DNA in relative units|
|4||Activity strength||Act strength||Guan, Chi||Fluorescence after DNA staining||of Zostera leaf surface extract against bacterial settlers; log(RU extract/RU control), RUextract = Fluorescence of stained DNA in bacteria settled on extract; RUcontrol = Fluorescence of stained DNA in bacteria settled on solvent control|
|5||Rosmarinic acid||C18H16O8||µg/ml||Guan, Chi||High Performance Liquid Chromatography (HPLC)||concentration in Zostera leaf surface extract|
180 data points