/* DATA DESCRIPTION: Citation: Wu, Fangli; Sokolov, Eugene P; Dellwig, Olaf; Sokolova, Inna M (2020): Combined effects of ZnO nanoparticles and elevated temperature on bioenergetics of the blue mussel Mytilus edulis in different seasons [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.918481 Abstract: Increased ZnO nanoparticles (nZnO) from multiple sources have raised concerns about the potential toxic effects on the marine organisms living in estuarine and coastal environments. The toxicity of nZnO and its interaction by common abiotic stressors (such as elevated temperature) are not well understood for marine organisms. Therefore, we examined the Input of ZnO nanoparticles (nZnO) from multiple sources have raised concerns about the potential toxic effects on the marine organisms in estuarine and coastal environments. The toxicity of nZnO and its interaction with common abiotic stressors (such as elevated temperature) are not well understood in marine organisms. Therefore, we examined the bioenergetics and oxidative stress responses of the blue mussel Mytilus edulis exposed for 21 days to different concentrations of nZnO or dissolved zinc (Zn2+) (0, 10, 100 ug l-1) and two temperatures (normal and high temperature) in winter and summer. In this data set, we measured the tissue levels of energy reserves (including proteins, lipids and carbohydrates), electron transport system (ETS) activity, cellular energy allocation (CEA) as a integrative index of energy balance, and the accumulation of Zn and levels of the metal-binding proteins (metallothioneins) as markers for intracellular Zn accumulation in the mussels. Our study provides new insights into the bioenergetics mechanisms underlying the combined effects of elevated temperature and nZnO of M. edulis and has important implications for understanding the effects of warming on the mussels' populations from polluted estuaries and coastal zones Keyword(s): Carbohydrate; energy reserves; Lipid; Mussel; nZnO; protein; warming Related to: Wu, Fangli; Sokolov, Eugene P; Dellwig, Olaf; Sokolova, Inna M (2021): Season-dependent effects of ZnO nanoparticles and elevated temperature on bioenergetics of the blue mussel Mytilus edulis. Chemosphere, 263, 127780, https://doi.org/10.1016/j.chemosphere.2020.127780 Parameter(s): Season (Season) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) Species (Species) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) Temperature, water [°C] (Temp) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) Experimental treatment (Exp treat) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) * COMMENT: Zn treatment Sample ID (Sample ID) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) Proteins, per wet mass [mg/g] (Proteins/wm) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) * COMMENT: in tissue Lipids, per wet mass [mg/g] (Lipids/wet m) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) * COMMENT: in tissue Carbohydrates, per wet mass [µmol/g] (CHO/wm) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) * COMMENT: in tissue Total energy reserve, per wet mass [kJ/g] (E reserve/wm) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) * COMMENT: Total energy reserve, per wet mass Electron transport system activity, per wet mass [mU/g] (ETS/wm) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) * COMMENT: Electron transport system activity, per wet mass Cellular energy allocation [days] (CEA) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) * COMMENT: Cellular energy allocation Metallothionein, per wet mass [µg/g] (MT/wm) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) * COMMENT: Metallothionein, per wet mass Zinc [µg/g] (Zn) * PI: Wu, Fangli (https://orcid.org/0000-0001-6443-0649, fangli.wu@uni-rostock.de) * COMMENT: wet mass of tissue License: Creative Commons Attribution 4.0 International (CC-BY-4.0) (URI: https://creativecommons.org/licenses/by/4.0/) Size: 1276 data points */ Season Species Temp [°C] Exp treat Sample ID Proteins/wm [mg/g] Lipids/wet m [mg/g] CHO/wm [µmol/g] E reserve/wm [kJ/g] ETS/wm [mU/g] CEA [days] MT/wm [µg/g] Zn [µg/g] summer Mytilus edulis 15 control s1-1 25.8860 45.8130 67.6982 2.6443 314.1072 12.0789 40.4553 5.4773 summer Mytilus edulis 15 control s1-2 22.7748 55.2154 21.0988 2.7941 333.6210 12.0167 39.3983 11.3195 summer Mytilus edulis 15 control s1-3 21.7170 48.3773 40.7776 2.5607 551.2797 6.6646 22.0054 10.4350 summer Mytilus edulis 15 control s1-4 17.4858 49.8731 40.8378 2.5184 527.7408 6.8469 38.1491 9.4925 summer Mytilus edulis 15 control s1-5 25.2638 53.5059 19.9754 2.7828 372.2794 10.7252 33.2483 summer Mytilus edulis 15 10 µg /l Zn as nZnO s2-1 22.9615 38.9749 19.8149 2.1531 254.0243 12.1611 31.4225 5.4461 summer Mytilus edulis 15 10 µg /l Zn as nZnO s2-2 19.0414 46.0267 18.1901 2.3324 258.9919 12.9214 26.5218 10.7974 summer Mytilus edulis 15 10 µg /l Zn as nZnO s2-3 26.0104 37.0517 19.0928 2.1480 278.6714 11.0594 33.5366 11.4677 summer Mytilus edulis 15 10 µg /l Zn as nZnO s2-4 18.0459 37.0517 10.8080 1.9307 193.8905 14.2874 26.1374 8.9717 summer Mytilus edulis 15 10 µg /l Zn as nZnO s2-5 17.2370 34.7011 34.5590 1.8933 316.9604 8.5707 27.6749 summer Mytilus edulis 15 100 µg /l Zn as nZnO s3-1 18.5436 33.1898 24.3284 1.8327 209.5831 12.5469 31.3264 11.7521 summer Mytilus edulis 15 100 µg /l Zn as nZnO s3-2 22.7748 32.7690 16.6856 1.8936 160.7220 16.9044 25.5608 16.9683 summer Mytilus edulis 15 100 µg /l Zn as nZnO s3-3 19.4770 33.4002 25.5521 1.8673 228.8677 11.7064 30.3655 21.0609 summer Mytilus edulis 15 100 µg /l Zn as nZnO s3-4 15.2458 39.7119 30.0255 2.0292 237.6311 12.2521 23.3507 11.0916 summer Mytilus edulis 15 100 µg /l Zn as nZnO s3-5 17.2370 33.6106 17.6685 1.7970 174.4658 14.7786 24.2155 summer Mytilus edulis 15 10 µg /l Zn2+ s4-1 20.1614 62.9082 57.2068 3.1491 283.5753 15.9335 24.8882 9.7399 summer Mytilus edulis 15 10 µg /l Zn2+ s4-2 32.9172 62.4808 21.5802 3.3260 247.3498 19.2934 38.1491 11.4773 summer Mytilus edulis 15 10 µg /l Zn2+ s4-3 23.0237 45.1720 30.7075 2.4337 314.5275 11.1019 31.2303 11.1959 summer Mytilus edulis 15 10 µg /l Zn2+ s4-4 27.5038 40.6845 30.4869 2.3632 273.9967 12.3753 43.3381 13.5648 summer Mytilus edulis 15 10 µg /l Zn2+ s4-5 15.7436 37.6928 32.3123 1.9686 307.2162 9.1939 25.9452 summer Mytilus edulis 15 100 µg /l Zn2+ s5-1 22.9615 51.2833 42.1217 2.7096 229.2880 16.9555 18.0655 13.2102 summer Mytilus edulis 15 100 µg /l Zn2+ s5-2 15.9303 40.1327 31.7306 2.0676 276.1494 10.7427 15.6520 summer Mytilus edulis 15 100 µg /l Zn2+ s5-3 18.2947 61.8028 18.7317 2.9393 192.4766 21.9111 39.6865 15.0667 summer Mytilus edulis 15 100 µg /l Zn2+ s5-4 25.2015 42.2366 17.1670 2.3273 246.4582 13.5488 32.9600 9.9551 summer Mytilus edulis 15 100 µg /l Zn2+ s5-5 20.5348 54.4392 17.3676 2.6979 264.8512 14.6158 33.7288 summer Mytilus edulis 20 control s6-1 20.9468 33.4190 20.7590 1.8882 243.0191 11.1482 24.1194 10.2441 summer Mytilus edulis 20 control s6-2 19.8254 37.2654 26.9897 2.0329 350.6002 8.3194 37.9569 7.3511 summer Mytilus edulis 20 control s6-3 24.5470 40.4708 44.6475 2.3285 296.5803 11.2648 33.5366 11.3604 summer Mytilus edulis 20 control s6-4 18.2909 45.8130 28.0497 2.3370 420.8730 7.9672 16.9124 8.2305 summer Mytilus edulis 20 control s6-5 16.8744 40.6845 44.6475 2.1528 247.1970 12.4954 31.6147 summer Mytilus edulis 20 10 µg /l Zn as nZnO s7-1 15.8711 32.0559 9.2284 1.6762 160.8366 14.9532 21.2366 10.9494 summer Mytilus edulis 20 10 µg /l Zn as nZnO s7-2 18.5860 57.9037 15.5366 2.7822 227.6576 17.5350 17.8734 10.2331 summer Mytilus edulis 20 10 µg /l Zn as nZnO s7-3 13.6873 37.0587 8.4787 1.8190 165.1037 15.8081 37.0920 9.4959 summer Mytilus edulis 20 10 µg /l Zn as nZnO s7-4 18.5860 32.0559 17.3205 1.7669 193.9160 13.0733 24.7921 10.4804 summer Mytilus edulis 20 10 µg /l Zn as nZnO s7-5 22.5403 38.7263 13.8303 2.1143 258.4570 11.7371 32.4795 summer Mytilus edulis 20 100 µg /l Zn as nZnO s8-1 20.5337 24.5517 10.7279 1.4964 98.1553 21.8742 17.2007 18.9135 summer Mytilus edulis 20 100 µg /l Zn as nZnO s8-2 22.0092 26.0108 11.6845 1.5925 127.4643 17.9258 22.9663 17.2498 summer Mytilus edulis 20 100 µg /l Zn as nZnO s8-3 13.4513 33.7235 21.4312 1.7225 130.6232 18.9201 19.4109 12.1988 summer Mytilus edulis 20 100 µg /l Zn as nZnO s8-4 17.2876 31.2221 13.0547 1.6893 148.2520 16.3496 19.7952 12.5540 summer Mytilus edulis 20 100 µg /l Zn as nZnO s8-5 19.7664 27.6784 14.2181 1.6125 119.4652 19.3667 32.9600 summer Mytilus edulis 20 10 µg /l Zn2+ s9-1 17.1695 41.6446 11.3484 2.0928 183.5604 16.3585 25.5608 11.6992 summer Mytilus edulis 20 10 µg /l Zn2+ s9-2 13.1562 39.3516 26.1107 1.9525 193.7631 14.4578 28.9241 14.8457 summer Mytilus edulis 20 10 µg /l Zn2+ s9-3 20.7697 41.2277 22.8014 2.1989 193.9032 16.2706 24.0233 10.3169 summer Mytilus edulis 20 10 µg /l Zn2+ s9-4 25.7864 44.7713 15.8727 2.4374 299.7138 11.6683 28.8280 10.2991 summer Mytilus edulis 20 10 µg /l Zn2+ s9-5 20.9468 40.3939 22.4395 2.1690 321.0491 9.6936 33.0561 summer Mytilus edulis 20 100 µg /l Zn2+ s10-1 12.2118 22.4672 12.8479 1.2210 162.5307 10.7792 27.6749 16.4114 summer Mytilus edulis 20 100 µg /l Zn2+ s10-2 21.8321 31.4305 11.0640 1.8004 197.6480 13.0695 36.3233 9.8604 summer Mytilus edulis 20 100 µg /l Zn2+ s10-3 15.8711 36.8502 12.7962 1.8768 193.9032 13.8878 31.1342 15.9683 summer Mytilus edulis 20 100 µg /l Zn2+ s10-4 22.0682 34.5573 25.2833 1.9744 219.5311 12.9039 21.6210 11.1787 summer Mytilus edulis 20 100 µg /l Zn2+ s10-5 19.0582 33.5150 16.6483 1.8337 128.8527 20.4189 24.9843 winter Mytilus edulis 10 control s1-1 26.0716 20.7837 45.9546 1.5916 285.7025 7.9928 30.8460 9.2121 winter Mytilus edulis 10 control s1-2 22.9576 21.1872 44.5793 1.5284 267.0803 8.2110 17.2968 9.0060 winter Mytilus edulis 10 control s1-3 20.0512 22.5321 30.7479 1.4682 139.0045 15.1546 25.6569 11.7081 winter Mytilus edulis 10 control s1-4 29.1164 19.5733 55.6340 1.6473 249.7063 9.4655 21.0444 8.9327 winter Mytilus edulis 10 control s1-5 24.2032 17.0180 59.1891 1.4397 209.6086 9.8549 18.7382 winter Mytilus edulis 10 10 µg /l Zn as nZnO s2-1 20.4096 15.9421 16.9425 1.1730 227.7850 7.3884 17.0085 12.1965 winter Mytilus edulis 10 10 µg /l Zn as nZnO s2-2 20.7926 13.9247 19.2780 1.1098 126.0505 12.6329 22.7741 12.0735 winter Mytilus edulis 10 10 µg /l Zn as nZnO s2-3 20.7379 14.3282 30.2030 1.1589 231.0968 7.1952 12.6843 9.7812 winter Mytilus edulis 10 10 µg /l Zn as nZnO s2-4 27.7399 27.6427 24.2085 1.8340 169.9440 15.4838 16.4320 11.1932 winter Mytilus edulis 10 10 µg /l Zn as nZnO s2-5 24.5124 20.9182 23.9231 1.4900 196.1323 10.9000 16.7202 winter Mytilus edulis 10 100 µg /l Zn as nZnO s3-1 24.5671 19.1698 10.4550 1.3798 137.9728 14.3486 28.2514 15.1399 winter Mytilus edulis 10 100 µg /l Zn as nZnO s3-2 17.5104 13.3868 8.3012 0.9752 89.2136 15.6839 24.5038 12.4402 winter Mytilus edulis 10 100 µg /l Zn as nZnO s3-3 18.3856 14.1937 13.1279 1.0433 204.6792 7.3135 23.6390 12.2004 winter Mytilus edulis 10 100 µg /l Zn as nZnO s3-4 16.4163 15.4041 8.0157 1.0277 208.7424 7.0641 19.8913 11.6198 winter Mytilus edulis 10 100 µg /l Zn as nZnO s3-5 21.6678 15.9421 18.3179 1.2075 202.1444 8.5707 25.6569 winter Mytilus edulis 10 10 µg /l Zn2+ s4-1 20.9020 19.4388 20.5755 1.3343 125.0697 15.3077 16.1437 9.6296 winter Mytilus edulis 10 10 µg /l Zn2+ s4-2 25.9347 19.1698 25.0389 1.4586 215.7863 9.6984 21.0444 10.1224 winter Mytilus edulis 10 10 µg /l Zn2+ s4-3 33.4837 16.3456 18.7331 1.5083 183.3311 11.8045 9.8015 11.6811 winter Mytilus edulis 10 10 µg /l Zn2+ s4-4 23.8560 22.5321 17.3577 1.5173 243.0191 8.9582 43.5303 9.1248 winter Mytilus edulis 10 10 µg /l Zn2+ s4-5 17.7839 19.4388 15.6191 1.2439 180.4142 9.8924 34.3053 winter Mytilus edulis 10 100 µg /l Zn2+ s5-1 20.6285 25.3564 16.0862 1.5474 160.9385 13.7952 19.3148 10.1897 winter Mytilus edulis 10 100 µg /l Zn2+ s5-2 25.4423 13.5213 12.2196 1.1832 171.3960 9.9051 27.3866 7.6834 winter Mytilus edulis 10 100 µg /l Zn2+ s5-3 14.8299 16.4800 17.7989 1.0630 161.0914 9.4678 25.6569 12.0070 winter Mytilus edulis 10 100 µg /l Zn2+ s5-4 23.6371 11.9074 12.9722 1.0785 185.3946 8.3469 33.7288 10.2182 winter Mytilus edulis 10 100 µg /l Zn2+ s5-5 19.6438 21.1872 15.4634 1.3571 212.8312 9.1489 23.3507 winter Mytilus edulis 15 control s6-1 25.2676 38.1961 87.3387 2.3905 197.5334 17.3638 10.7202 winter Mytilus edulis 15 control s6-2 31.0502 48.8938 35.1938 2.7875 179.8537 22.2373 31.9991 9.2436 winter Mytilus edulis 15 control s6-3 37.3835 33.5449 55.0512 2.3958 281.5755 12.2080 55.9263 9.4542 winter Mytilus edulis 15 control s6-4 29.9488 54.6303 34.6550 2.9859 221.6710 19.3269 74.6645 11.4042 winter Mytilus edulis 15 control s6-5 32.7712 38.1961 36.5407 2.4105 284.8746 12.1405 63.1333 winter Mytilus edulis 15 10 µg /l Zn as nZnO s7-1 25.4742 25.4828 15.2979 1.6662 160.4290 14.9015 12.0107 winter Mytilus edulis 15 10 µg /l Zn as nZnO s7-2 34.3545 22.3820 19.4926 1.7701 225.0337 11.2858 32.8639 11.9912 winter Mytilus edulis 15 10 µg /l Zn as nZnO s7-3 32.0828 34.1650 12.8350 2.1600 178.4526 17.3667 37.4764 9.4695 winter Mytilus edulis 15 10 µg /l Zn as nZnO s7-4 30.5683 52.9249 12.0653 2.8622 244.8915 16.7695 41.2240 9.2434 winter Mytilus edulis 15 10 µg /l Zn as nZnO s7-5 26.1626 25.7929 15.2594 1.6948 157.4357 15.4460 36.6116 winter Mytilus edulis 15 100 µg /l Zn as nZnO s8-1 18.2558 43.3124 14.8361 2.1958 143.6919 21.9252 19.0265 18.3959 winter Mytilus edulis 15 100 µg /l Zn as nZnO s8-2 20.4014 28.4286 15.7597 1.6622 142.2526 16.7659 50.4490 13.2265 winter Mytilus edulis 15 100 µg /l Zn as nZnO s8-3 33.2059 31.5294 18.6844 2.1013 119.2996 25.2716 76.6825 13.8379 winter Mytilus edulis 15 100 µg /l Zn as nZnO s8-4 22.5470 37.8860 14.1049 2.0821 121.8980 24.5073 47.8545 11.7800 winter Mytilus edulis 15 100 µg /l Zn as nZnO s8-5 21.3012 29.5138 13.7201 1.7203 108.9567 22.6536 36.0350 winter Mytilus edulis 15 10 µg /l Zn2+ s9-1 35.5248 47.0334 23.0330 2.7830 117.6437 33.9423 17.0085 13.2250 winter Mytilus edulis 15 10 µg /l Zn2+ s9-2 35.1118 51.0644 22.9946 2.9322 221.7729 18.9706 65.4396 8.7436 winter Mytilus edulis 15 10 µg /l Zn2+ s9-3 31.8763 44.0876 31.0376 2.6043 203.6347 18.3501 47.5662 12.9529 winter Mytilus edulis 15 10 µg /l Zn2+ s9-4 36.3509 46.2582 16.9142 2.7529 188.0312 21.0068 54.7732 10.0839 winter Mytilus edulis 15 10 µg /l Zn2+ s9-5 36.0755 40.8318 28.9210 2.5698 146.2012 25.2202 40.6475 winter Mytilus edulis 15 100 µg /l Zn2+ s10-1 35.3871 41.4519 22.5712 2.5578 149.7550 24.5063 26.2335 14.2536 winter Mytilus edulis 15 100 µg /l Zn2+ s10-2 23.3401 48.4287 23.9566 2.5486 103.1994 35.4341 66.8810 10.4315 winter Mytilus edulis 15 100 µg /l Zn2+ s10-3 21.1372 45.0178 34.9244 2.3956 93.7099 36.6794 54.4849 13.9738 winter Mytilus edulis 15 100 µg /l Zn2+ s10-4 17.9706 40.2116 30.5373 2.1159 173.7218 51.3138 10.6678 winter Mytilus edulis 15 100 µg /l Zn2+ s10-5 22.5829 27.8084 24.3415 1.7172 139.9726 17.6019 16.7202