Citation:

PANGAEA.917678

Name: Seawater carbonate chemistry and growth and enzyme activities of Nitzschia closterium
Published: 2020-05-20
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; Nitzschia closterium; Not applicable; Ochrophyta; Other metabolic rates; Pelagos; Phytoplankton; Single species

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Abstract:

In this study, Nitzschia closterium was incubated in seawater at different pH values (8.10, 7.71, and 7.45) and using different nitrogen forms (NO3–N and NH4–N) in the laboratory. The results showed that the growth of N. closterium was inhibited by ocean acidification, with individuals under lower pH levels showing lower growth rates and lower nitrogen uptake rates for both nitrogen forms. The Vmax/Ks ratio decreased with decreasing pH, indicating the inhibition of nitrogen uptake, whereas the ratios for NH4–N cultures were higher than those for NO3–N cultures, implying the highly competitive position of NH4–N. Acidification might induce reactive oxygen species based on the result that the maximum enzyme activities of SuperOxide Dismutase (SOD) and CATalase (CAT) increased under lower pH levels. The SOD and CAT activities for the NO3–N cultures were higher than those for NH4–N cultures at the low pH level, indicating that acidification might cause more oxidative stress for NO3–N cultures than for NH4–N cultures. Thus, ocean acidification might have a more detrimental effect on the growth of N. closterium under NO3–N conditions than NH4–N conditions, with a lower ratio (γ) of the maximum growth rate to the maximum nutrient uptake rate, and a drop in nitrate reductase activity under lower pH levels.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; Nitzschia closterium; Not applicable; Ochrophyta; Other metabolic rates; Pelagos; Phytoplankton; Single species

Supplement to:

Li, Keqiang; Li, Min; He, Yunfeng; Gu, Xingyan; Pang, Kai; Ma, Yunpeng (2020): Effects of pH and nitrogen form on Nitzschia closterium growth by linking dynamic with enzyme activity. Chemosphere, 249, 126154, https://doi.org/10.1016/j.chemosphere.2020.126154

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-05-6.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Li, Keqiang		study
2	Species	Species		Li, Keqiang
3	Registration number of species	Reg spec no		Li, Keqiang
4	Uniform resource locator/link to reference	URL ref		Li, Keqiang		WoRMS Aphia ID
5	Treatment	Treat		Li, Keqiang
6	Time in hours	Time	h	Li, Keqiang
7	pH	pH		Li, Keqiang
8	pH, standard deviation	pH std dev	±	Li, Keqiang
9	Chlorophyll a	Chl a	µg/l	Li, Keqiang
10	Chlorophyll a, standard deviation	Chl a std dev	±	Li, Keqiang
11	Nitrogen, particulate	PN	µmol/l	Li, Keqiang
12	Nitrogen, particulate, standard deviation	PN std dev	±	Li, Keqiang
13	Biomass	Biom	g/l	Li, Keqiang		Final
14	Biomass, standard deviation	Biom std dev	±	Li, Keqiang		Final
15	Growth rate, maximum	µ max	µg/l/h	Li, Keqiang
16	Growth rate, maximum, standard deviation	µ max std dev	±	Li, Keqiang
17	Nitrogen, inorganic, dissolved	DIN	µmol/l	Li, Keqiang
18	Dissolved inorganic nitrogen, standard deviation	DIN std dev	±	Li, Keqiang
19	Nitrate	[NO3]-	µmol/l	Li, Keqiang
20	Nitrite	[NO2]-	µmol/l	Li, Keqiang
21	Ammonium	[NH4]+	µmol/l	Li, Keqiang
22	Maximum nutrient uptake rate	Vmax	µmol/L/h	Li, Keqiang
23	Maximum nutrient uptake rate, standard deviation	Vmax std dev	±	Li, Keqiang
24	Half saturation concentration	Ks	µmol/l	Li, Keqiang
25	Half saturation concentration, standard deviation	Ks std dev	±	Li, Keqiang
26	Minimum concentration threshold	Cmin	µmol/l	Li, Keqiang
27	Minimum concentration threshold, standard deviation	Cmin std dev	±	Li, Keqiang
28	Ratio	Ratio		Li, Keqiang		maximum growth rate to the maximum nutrient uptake rate
29	Ratio, standard deviation	Ratio std dev	±	Li, Keqiang		maximum growth rate to the maximum nutrient uptake rate
30	Ratio	Ratio		Li, Keqiang		Vmax/Ks (1/h)
31	Ratio, standard deviation	Ratio std dev	±	Li, Keqiang		Vmax/Ks
32	Superoxide dismutase activity, unit per protein mass	SOD/prot	U/mg	Li, Keqiang
33	Superoxide dismutase activity, standard deviation	SOD std dev	±	Li, Keqiang
34	Catalase activity, unit per protein mass	CAT/prot	U/mg	Li, Keqiang
35	Catalase activity, standard deviation	CAT std dev	±	Li, Keqiang
36	Nitrate reductase activity	NR	µg/mg/h	Li, Keqiang
37	Nitrate reductase activity, standard deviation	NO3 reduct act std dev	±	Li, Keqiang
38	Salinity	Sal		Li, Keqiang
39	Temperature, water	Temp	°C	Li, Keqiang
40	Temperature, water, standard deviation	Temp std dev	±	Li, Keqiang
41	Carbon, inorganic, dissolved	DIC	µmol/kg	Li, Keqiang
42	Alkalinity, total	AT	µmol/kg	Li, Keqiang
43	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
45	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3075 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 Time [h]	7 pH	8 pH std dev [±]	9 Chl a [µg/l]	10 Chl a std dev [±]	11 PN [µmol/l]	12 PN std dev [±]	13 Biom [g/l] (Final)	14 Biom std dev [±] (Final)	15 µ max [µg/l/h]	16 µ max std dev [±]	17 DIN [µmol/l]	18 DIN std dev [±]	19 [NO3]- [µmol/l]	20 [NO2]- [µmol/l]	21 [NH4]+ [µmol/l]	22 Vmax [µmol/L/h]	23 Vmax std dev [±]	24 Ks [µmol/l]	25 Ks std dev [±]	26 Cmin [µmol/l]	27 Cmin std dev [±]	28 Ratio (maximum growth rate to the ma...)	29 Ratio std dev [±] (maximum growth rate to the ma...)	30 Ratio (Vmax/Ks (1/h))	31 Ratio std dev [±] (Vmax/Ks)	32 SOD/prot [U/mg]	33 SOD std dev [±]	34 CAT/prot [U/mg]	35 CAT std dev [±]	36 NR [µg/mg/h]	37 NO3 reduct act std dev [±]	38 Sal	39 Temp [°C]	40 Temp std dev [±]	41 DIC [µmol/kg]	42 AT [µmol/kg]	43 CSC flag	44 pH (total scale)	45 CO2 [µmol/kg]	46 fCO2water_SST_wet [µatm]	47 pCO2water_SST_wet [µatm]	48 [HCO3]- [µmol/kg]	49 [CO3]2- [µmol/kg]	50 Omega Arg	51 Omega Cal
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	0.00	8.070	0.03121	8.78216	0.71760	6.95317	0.00000																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	20.50	8.040	0.03310	24.00000	0.00000	18.67893	0.29093																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	39.00	8.110	0.03151	29.91335	0.00216	30.19898	1.45463																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	45.50	8.110	0.02828	30.69927	2.07672	41.13739	3.54132																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	68.00	8.170	0.04243	30.84181	0.14852	46.44030	0.70711																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	92.00	8.110	0.02828	15.47618	0.38019	47.67607	0.01230																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	116.50	8.160	0.03321	8.13933	3.07243	29.99327	1.16370																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	0.00	8.070	0.03121	8.71178	0.02705	10.03890	0.87278																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	20.50	8.040	0.03310	18.36497	4.04860	22.17609	0.58185																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	39.00	8.110	0.03151	39.30097	0.23453	40.69045	1.74555																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	45.50	8.110	0.02828	53.67809	0.40167	57.67210	3.85000																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	68.00	8.170	0.04243	41.65135	4.86266	62.06975	6.51538																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	92.00	8.110	0.02828	27.78932	1.08834	60.18149	4.43728																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	116.50	8.160	0.03321	23.22776	0.20973	49.74192	12.80071																										27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	0.00	7.730	0.03111	9.17379	0.83865	7.36460	0.00000																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	20.50	7.700	0.03410	18.15847	0.13755	17.23893	0.00000																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	39.00	7.640	0.03151	27.12393	1.53808	30.19898	2.03648																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	45.50	7.670	0.02828	30.90022	3.11514	32.75206	0.00000																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	68.00	7.770	0.03243	21.47374	1.65831	44.26972	1.48077																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	92.00	7.720	0.02818	13.89540	1.57323	44.73136	4.17675																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	116.50	7.710	0.02921	5.63369	1.87822	43.82464	0.22240																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	0.00	7.730	0.03111	8.66110	0.00000	9.01032	0.58185																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	20.50	7.700	0.03410	18.00663	2.33239	25.46753	5.23665																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	39.00	7.640	0.03151	37.49088	1.60283	34.51900	4.07295																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	45.50	7.670	0.02828	48.20844	1.24639	31.91441	1.18461																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	68.00	7.770	0.03243	33.33688	1.91142	46.15443	0.59231																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	92.00	7.720	0.02818	12.30144	2.77995	49.92385	1.18461																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	116.50	7.710	0.02921	6.16693	1.33613	39.86759	0.58185																										27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	0.00	7.455	0.02121	8.78216	0.80381	5.71888	0.58185																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	20.50	7.475	0.02121	17.03629	2.89830	16.62178	0.29093																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	39.00	7.460	0.02121	22.54993	3.91260	26.29039	1.16370																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	45.50	7.485	0.02828	24.60910	1.00589	29.05087	1.08812																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	68.00	7.505	0.04243	24.15420	1.06433	42.80384	2.36923																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	92.00	7.485	0.02828	20.82187	0.55738	42.17560	2.66538																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	116.50	7.465	0.02121	11.43747	4.34383	41.74476	0.74752																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	0.00	7.455	0.02121	8.68644	0.32390	10.65604	0.00000																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	20.50	7.475	0.02121	18.44076	0.45734	33.28471	3.49110																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	39.00	7.460	0.02121	23.01573	0.00000	36.37044	0.87278																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	45.50	7.485	0.02828	31.84255	0.24648	43.64149	5.33076																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	68.00	7.505	0.04243	29.00000	1.55203	41.75678	3.25769																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	92.00	7.485	0.02828	15.35350	0.79871	41.96619	8.29230																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	116.50	7.465	0.02121	8.76669	2.60314	42.33618	3.49110																										27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N		8.100						0.000031230	0.0000002	31.230	0.16971						0.71	0.01	0.29	0.09	3.65	0.07	0.95	0.02	2.45	0.72	2.17	0.15	0.72359	0.12978	24.04037	4.10483	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N		7.710						0.000026695	0.0000023	26.695	2.28395						0.63	0.01	0.36	0.04	3.72	0.01	0.84	0.01	1.74	0.17	5.29	0.42	2.10638	0.01909	32.39791	0.49990	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N		7.450						0.000025420	0.0000001	25.420	0.10414						0.53	0.07	0.41	0.40	4.20	1.84	0.72	0.06	1.28	1.09	7.56	0.31	3.77186	0.89944	54.56417	5.87870	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N		8.100						0.000048860	0.0000032	48.860	3.18198						1.03	0.01	0.11	0.01	2.62	0.01	1.04	0.07	9.61	0.76	2.52	0.04	1.12236	0.19441			27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N		7.710						0.000041595	0.0000008	41.595	0.82731						0.80	0.01	0.20	0.03	2.77	0.06	1.21	0.06	4.00	0.55	4.90	0.53	1.85277	0.33256			27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N		7.450						0.000030180	0.0000007	30.180	0.66468						0.63	0.03	0.27	0.02	2.88	2.25	1.15	0.03	2.31	0.06	5.74	0.08	2.40246	0.09571			27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	0.01	8.100										31.75500	0.70780	30.1115	0.5595	1.08400																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	20.50	8.100										18.52130	1.09060	16.2305	1.0310	1.25980																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	39.00	8.100										4.41795	2.39445	2.2550	0.5740	1.58895																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	45.50	8.100										3.34925	0.20205	0.8590	0.2315	2.25875																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	68.00	8.100										5.27190	2.39350	0.7625	0.1175	4.39190																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	92.00	8.100										3.79855	1.13085	0.9315	0.1555	2.71155																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	116.50	8.100										5.86770	1.82890	0.7735	0.1535	4.94070																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	165.00	8.100										1.02240	0.56930	0.6000	0.1130	0.30940																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	237.00	8.100										3.88880	0.77930	0.4065	0.1685	3.31380																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	0.01	7.710										33.84215	2.21735	30.4405	0.3700	3.03165																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	20.50	7.710										20.00840	1.51700	17.1015	0.6990	2.20790																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	39.00	7.710										13.92015	3.87735	7.0115	0.6740	6.23465																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	45.50	7.710										3.29060	0.60260	0.8855	0.7595	1.64560																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	68.00	7.710										5.25015	2.64155	0.8025	0.1845	4.26315																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	92.00	7.710										4.81100	1.96290	0.6250	0.1605	4.02550																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	116.50	7.710										2.79080	0.93540	0.3870	0.2880	2.11580																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	165.00	7.710										1.48995	0.14855	0.3000	0.1240	1.06595																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	237.00	7.710										4.19325	0.51285	1.1175	0.2315	2.84425																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	0.01	7.450										34.47800	0.80250	33.0080	0.5950	1.41050																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	20.50	7.450										24.63130	1.22190	22.2985	0.9010	1.43180																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	39.00	7.450										14.56230	4.64530	9.3700	0.6670	4.52530																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	45.50	7.450										10.73145	4.57165	6.8635	0.4080	3.45995																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	68.00	7.450										6.97720	2.20225	0.3290	0.2205	4.50145																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	92.00	7.450										4.41990	1.87260	0.3635	0.1430	3.91340																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	116.50	7.450										5.09070	1.07590	0.8105	0.1375	4.14270																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	165.00	7.450										2.12575	0.71065	0.7995	0.1515	1.17475																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	237.00	7.450										3.49585	0.37645	0.7470	0.1925	2.55635																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	0.01	8.100										32.00000	0.59670	0.4880	0.3570	30.02290																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	20.50	8.100										9.08790	1.96940	0.4685	0.3585	8.26090																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	39.00	8.100										3.21770	1.58330	0.5730	0.1885	2.45620																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	45.50	8.100										2.67685	1.10175	0.4900	0.1870	1.99985																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	68.00	8.100										1.54830	0.32160	0.3595	0.1950	0.99380																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	92.00	8.100										4.53520	0.77835	0.6250	0.1430	3.76720																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	116.50	8.100										3.41980	1.23295	0.6285	0.2250	2.56630																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	165.00	8.100										1.49440	0.39900	0.5500	0.1625	0.78190																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	237.00	8.100										2.44585	0.19045	0.5675	0.1870	1.69135																	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	0.01	7.710										33.71155	4.09715	2.1235	0.4270	31.16105																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	20.50	7.710										14.76325	3.02305	2.1860	0.4700	12.10725																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	39.00	7.710										3.84045	2.30165	1.1097	0.3490	2.38175																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	45.50	7.710										1.79945	0.13915	0.3960	0.1675	1.23595																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	68.00	7.710										5.69770	0.11200	0.4060	0.1320	5.15970																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	92.00	7.710										2.72585	0.52155	0.4530	0.1545	2.11835																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	116.50	7.710										3.49095	0.65705	0.5560	0.2135	2.72145																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	165.00	7.710										2.22480	0.26230	0.5775	0.2310	1.41630																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	237.00	7.710										3.84360	1.41540	0.5655	0.1715	3.10660																	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	0.01	7.450										30.60260	0.21330	0.5845	0.1890	29.82910																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	20.50	7.450										22.15140	2.08180	0.6760	0.2060	21.26940																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	39.00	7.450										4.75620	0.35220	0.7090	0.1700	3.97720																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	45.50	7.450										3.71025	0.68125	0.3755	0.1780	3.15675																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	68.00	7.450										2.37760	0.87700	0.3060	0.1735	1.89810																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	92.00	7.450										2.33530	0.16670	0.6055	0.1615	1.56830																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	116.50	7.450										3.01295	0.35895	0.4915	0.2150	2.30945																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	165.00	7.450										3.72790	0.16800	0.7150	0.1460	2.86690																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NH4-N	237.00	7.450										3.33140	0.99920	0.6435	0.1515	2.53640																	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	0.01	8.100																													11.82022	1.16248	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	23.00	8.100																													37.33428	3.07671	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	48.00	8.100																													54.56417	5.87870	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	72.00	8.100																													28.17461	6.13737	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	96.00	8.100																													15.07854	8.82014	27	20	1	1828	2314	15	8.51	3.80	112.29	112.68	1470.62	353.58	5.68	8.94
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	0.01	7.710																													10.69406	0.13798	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	23.00	7.710																													22.83938	0.41533	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	48.00	7.710																													32.39791	0.49990	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	72.00	7.710																													19.04749	2.50514	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	96.00	7.710																													9.74522	2.87464	27	20	1	1860	2354	15	8.52	3.84	113.59	113.98	1494.95	361.20	5.80	9.14
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	0.01	7.450																													10.76141	0.45064	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	23.00	7.450																													16.38382	0.99277	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	48.00	7.450																													24.04037	4.10483	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	72.00	7.450																													15.65938	3.64948	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	NO3-N	96.00	7.450																													6.36917	2.77334	27	20	1	1904	2366	15	8.47	4.46	131.91	132.36	1560.58	338.96	5.45	8.57