Citation:

PANGAEA.916160

Name: Seawater carbonate chemistry and photosynthetic pigments and photophysiology of polar microalga Chlorella sp.
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chlorella sp.; Chlorophyta; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Phytoplankton; Plantae; Primary production/Photosynthesis; Single species

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Abstract:

Ocean acidification, due to increased levels of anthropogenic carbon dioxide, is known to affect the physiology and growth of marine phytoplankton, especially in polar regions. However, the effect of acidification or carbonation on cellular metabolism in polar marine phytoplankton still remains an open question. There is some evidence that small chlorophytes may benefit more than other taxa of phytoplankton. To understand further how green polar picoplankton could acclimate to high oceanic CO2, studies were conducted on an Antarctic Chlorella sp. Chlorella sp. maintained its growth rate (∼0.180 /day), photosynthetic quantum yield (Fv/Fm = ∼0.69) and chlorophyll a (0.145 fg/cell) and carotenoid (0.06 fg/cell) contents under high CO2, while maximum rates of electron transport decreased and non-photochemical quenching increased under elevated CO2. GCMS-based metabolomic analysis reveal that this polar Chlorella strain modulated the levels of metabolites associated with energy, amino acid, fatty acid and carbohydrate production, which could favour its survival in an increasingly acidified ocean.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chlorella sp.; Chlorophyta; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Phytoplankton; Plantae; Primary production/Photosynthesis; Single species

Supplement to:

Tan, Yong Hao; Lim, Phaik Eem; Beardall, John; Poong, Sze Wan; Phang, Siew Moi (2019): A metabolomic approach to investigate effects of ocean acidification on a polar microalga Chlorella sp. Aquatic Toxicology, 217, 105349, https://doi.org/10.1016/j.aquatox.2019.105349

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-05-6.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Lim, Phaik Eem		study
2	Species	Species		Lim, Phaik Eem
3	Day of experiment	DOE	day	Lim, Phaik Eem
4	Treatment	Treat		Lim, Phaik Eem
5	Cell density	Cells	#/ml	Lim, Phaik Eem
6	Cell density, standard deviation	Cells std dev	±	Lim, Phaik Eem
7	Growth rate	µ	1/day	Lim, Phaik Eem
8	Growth rate, standard deviation	µ std dev	±	Lim, Phaik Eem
9	Chlorophyll a per cell	Chl a/cell	pg/#	Lim, Phaik Eem
10	Chlorophyll a, standard deviation	Chl a std dev	±	Lim, Phaik Eem
11	Carotenoids per cell	Carotenoids/cell	pg/#	Lim, Phaik Eem
12	Carotenoids, standard deviation	Carotenoids std dev	±	Lim, Phaik Eem
13	Ratio	Ratio		Lim, Phaik Eem		chlorophyll a/carotenoid
14	Ratio, standard deviation	Ratio std dev	±	Lim, Phaik Eem		chlorophyll a/carotenoid
15	Diameter	Ø	µm	Lim, Phaik Eem
16	Diameter, standard deviation	Ø std dev	±	Lim, Phaik Eem
17	Surface area	SA	µm²	Lim, Phaik Eem
18	Surface area, standard deviation	SA std dev	±	Lim, Phaik Eem
19	Volume	Vol	µm³	Lim, Phaik Eem
20	Cell biovolume, standard deviation	Cell biovol std dev	±	Lim, Phaik Eem
21	Cell surface area/cell volume ratio	Cell SA/cell vol	1/µm	Lim, Phaik Eem
22	Cell surface area/cell volume, standard deviation	Cell SA/cell vol std dev	±	Lim, Phaik Eem
23	Photochemical quantum yield	Fv/Fm		Lim, Phaik Eem
24	Photochemical quantum yield, standard deviation	Fv/Fm std dev	±	Lim, Phaik Eem
25	Maximum light utilization efficiency	Alpha	electrons/photons	Lim, Phaik Eem
26	Maximum light utilization efficiency, standard deviation	Alpha std dev	±	Lim, Phaik Eem
27	Maximal electron transport rate, relative	rETR max	µmol e/m²/s	Lim, Phaik Eem
28	Maximal electron transport rate, relative, standard deviation	rETR max std dev	±	Lim, Phaik Eem
29	Non photochemical quenching, maximum	NPQ max		Lim, Phaik Eem
30	Non photochemical quenching, standard deviation	NPQ std dev	±	Lim, Phaik Eem
31	Light saturation	Ek	µmol/m²/s	Lim, Phaik Eem
32	Light saturation, standard deviation	Ek std dev	±	Lim, Phaik Eem
33	Temperature, water	Temp	°C	Lim, Phaik Eem
34	Salinity	Sal		Lim, Phaik Eem
35	Salinity, standard deviation	Sal std dev	±	Lim, Phaik Eem
36	pH	pH		Lim, Phaik Eem	Potentiometric	NBS scale
37	pH, standard deviation	pH std dev	±	Lim, Phaik Eem	Potentiometric	NBS scale
38	Alkalinity, total	AT	µmol/kg	Lim, Phaik Eem	Potentiometric titration
39	Alkalinity, total, standard deviation	AT std dev	±	Lim, Phaik Eem	Potentiometric titration
40	Carbon, inorganic, dissolved	DIC	µmol/kg	Lim, Phaik Eem	Calculated using CO2SYS
41	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Lim, Phaik Eem	Calculated using CO2SYS
42	Bicarbonate ion	[HCO3]-	µmol/kg	Lim, Phaik Eem	Calculated using CO2SYS
43	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Lim, Phaik Eem	Calculated using CO2SYS
44	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Lim, Phaik Eem	Calculated using CO2SYS
45	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Lim, Phaik Eem	Calculated using CO2SYS
46	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
52	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
55	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2064 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 DOE [day]	4 Treat	5 Cells [#/ml]	6 Cells std dev [±]	7 µ [1/day]	8 µ std dev [±]	9 Chl a/cell [pg/#]	10 Chl a std dev [±]	11 Carotenoids/cell [pg/#]	12 Carotenoids std dev [±]	13 Ratio (chlorophyll a/carotenoid)	14 Ratio std dev [±] (chlorophyll a/carotenoid)	15 Ø [µm]	16 Ø std dev [±]	17 SA [µm²]	18 SA std dev [±]	19 Vol [µm³]	20 Cell biovol std dev [±]	21 Cell SA/cell vol [1/µm]	22 Cell SA/cell vol std dev [±]	23 Fv/Fm	24 Fv/Fm std dev [±]	25 Alpha [electrons/photons]	26 Alpha std dev [±]	27 rETR max [µmol e/m²/s]	28 rETR max std dev [±]	29 NPQ max	30 NPQ std dev [±]	31 Ek [µmol/m²/s]	32 Ek std dev [±]	33 Temp [°C]	34 Sal	35 Sal std dev [±]	36 pH (NBS scale, Potentiometric)	37 pH std dev [±] (NBS scale, Potentiometric)	38 AT [µmol/kg] (Potentiometric titration)	39 AT std dev [±] (Potentiometric titration)	40 DIC [µmol/kg] (Calculated using CO2SYS)	41 DIC std dev [±] (Calculated using CO2SYS)	42 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	43 [HCO3]- std dev [±] (Calculated using CO2SYS)	44 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	45 pCO2 std dev [±] (Calculated using CO2SYS)	46 CSC flag (Calculated using seacarb afte...)	47 pH (Calculated using seacarb afte...)	48 CO2 [µmol/kg] (Calculated using seacarb afte...)	49 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	50 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	51 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	52 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	53 DIC [µmol/kg] (Calculated using seacarb afte...)	54 Omega Arg (Calculated using seacarb afte...)	55 Omega Cal (Calculated using seacarb afte...)
laboratory	Chlorella sp. (phytoplankton)	0	Ambient CO2	648896	79090																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	5	Ambient CO2	1241800	102574																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	9	Ambient CO2	1808945	345187																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	12	Ambient CO2	3356000	184673																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	14	Ambient CO2	6411111	376738																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	15	Ambient CO2	8183333	253585																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	18	Ambient CO2	10746667	415093																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	21	Ambient CO2	16695003	56817																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	21	Ambient CO2	569200	156160																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	23	Ambient CO2	746267	79090																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	23	Ambient CO2	648896	102574																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	25	Ambient CO2	1241800	39502																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	25	Ambient CO2	731236	74173																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	27	Ambient CO2	1023352	38679																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	27	Ambient CO2	637646	99465																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	29	Ambient CO2	1026188	61746																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	29	Ambient CO2	493000	81509																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	31	Ambient CO2	807810	37625																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	31	Ambient CO2	521366	83652																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	33	Ambient CO2	841843	41488																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	33	Ambient CO2	541219	149910																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	35	Ambient CO2	1051713	90310																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	35	Ambient CO2	697203	345187																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	37	Ambient CO2	1808945	67472																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	37	Ambient CO2	765269	166098																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	39	Ambient CO2	1139631	69816																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	39	Ambient CO2	552563	333453																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	41	Ambient CO2	1099926	45377																											4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	41	Ambient CO2																													4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	43	Ambient CO2																													4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	43	Ambient CO2																													4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	45	Ambient CO2																													4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	45	Ambient CO2																													4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	47	Ambient CO2																													4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)	0	High CO2	206034	28132																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	5	High CO2	775442	187880																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	9	High CO2	1518600	382998																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	12	High CO2	2951210	481793																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	14	High CO2	5973333	316874																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	15	High CO2	7633333	407567																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	18	High CO2	10516000	354065																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	21	High CO2	15876706	500348																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	21	High CO2	487334	8968																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	23	High CO2	617793	22689																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	23	High CO2	371054	20841																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	25	High CO2	498678	16537																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	25	High CO2	430612	28361																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	27	High CO2	810646	83363																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	27	High CO2	714220	94062																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	29	High CO2	997827	169454																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	29	High CO2	458973	46601																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	31	High CO2	793630	72639																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	31	High CO2	583760	45200																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	33	High CO2	827663	114852																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	33	High CO2	595104	43569																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	35	High CO2	856024	88783																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	35	High CO2	478825	35423																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	37	High CO2	915581	66753																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	37	High CO2	555399	35195																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	39	High CO2	1518600	382998																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	39	High CO2	929762	86209																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	41	High CO2	2668276	350964																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	41	High CO2	585118	55080																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	43	High CO2	859035	90219																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	43	High CO2	599165	88424																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	45	High CO2	2094485	489798																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	45	High CO2	697083	82763																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)	47	High CO2	1647839	282889																											4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36
laboratory	Chlorella sp. (phytoplankton)		Ambient CO2			0.188855702	0.020549938	0.0000001453	0.00000001779	0.0000000643	0.00000000844	2.266446541	0.149529968	4.89	0.60	66.48	17.99	52.33	21.75	1.34	0.17	0.69	0.02	0.73	0.06	93.05	8.45	2.21	0.08	129.68	21.03	4	33.55400000	0.376010638	8.16	0.01	2434	30.3	2283.8	25.9	2147.0	23.1	402.1	11.9	26	8.06	21.70	397.94	399.60	2146.79	115.97	2284.45	1.76	2.79
laboratory	Chlorella sp. (phytoplankton)		High CO2			0.180458300	0.018229987	0.0000001454	0.00000002139	0.0000000609	0.00000000931	2.392770811	0.165884641	5.08	0.54	82.06	17.05	71.03	21.65	1.19	0.13	0.68	0.02	0.75	0.06	71.27	3.51	2.56	0.22	95.66	12.12	4	33.32599988	0.251443800	7.81	0.01	2488	36.9	2457.1	35.3	2347.7	33.8	975.2	22.6	26	7.71	53.25	975.47	979.55	2348.86	56.37	2458.48	0.85	1.36