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von Jackowski, Anabel; Grosse, Julia; Nöthig, Eva-Maria; Engel, Anja (2020): Organic matter and bacteria measurements of POLARSTERN cruise PS114 and Maria S. Merian cruise MSM77. PANGAEA, https://doi.org/10.1594/PANGAEA.915751

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Abstract:
The discrete biogeochemical measurements from RV Polarstern PS114 and RV Maria S. Merian MSM77 sampled for dissolved organic matter, heterotrophic bacteria and gel particles. The parameters include chlorophyll, DOC/TDN/TDP/DOP, dissolved hydrolysable amino acids, dissolved combined carbohydrates, heterotrophic bacterial abundance and production, gel particles like TEP and CSP. The samples were aquired within 0-100 m of the Greenland Sea between 78°N to 80°N and 2°W to 14°E. The data was collected daily on board PS114 from 16th July 2018-23rd July 2018 and on board MSM77 from 16th September 2018-4th October 2018. The water collection occured using a CTD and laboratory methods varied by parameter. Chlorophyll was extracted using acetone, DOC/TDN was analysed using High-Temperature Catalytic Oxidation (TOC-VCSH), TDP/DOP was analysed colorimetrically using Acidic Molybdate Solution, dissolved hydrolysable amino acids were analysed using High Performal Liquid Chromatography (HPLC) Ortho-phthaldialdehyde Derivatization, dissolved combined carbohydrates were analysed using High Performance Anion Exchange Chromatography (HPAEC) coupled with Pulsed Amperometric Detection (PAD), cell abundance was analysed using flow cytometery, bacterial production was analysed using radioactively labelled 3H-Leucine and apllication of the microcentrifuge method, gel particles were analysed microscopically followed by image analysis. The purpose of data collection was to understand seasonal cycling of organic mater and heterotrophic bacteria dynamics within microbial loop.
Keyword(s):
Arctic; Bacteria; CSP; Greenland Sea; organic carbon; TEP
Supplement to:
von Jackowski, Anabel; Grosse, Julia; Nöthig, Eva-Maria; Engel, Anja (2020): Dynamics of organic matter and bacterial activity in the Fram Strait during summer and autumn. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences, 378(2181), 20190366, https://doi.org/10.1098/rsta.2019.0366
Related to:
von Jackowski, Anabel; Engel, Anja (2019): Physical oceanography measured on water bottle samples during Maria S. Merian cruise MSM77. PANGAEA, https://doi.org/10.1594/PANGAEA.907467
Coverage:
Median Latitude: 79.123042 * Median Longitude: 4.708270 * South-bound Latitude: 78.187393 * West-bound Longitude: -1.836833 * North-bound Latitude: 80.300000 * East-bound Longitude: 13.999000
Date/Time Start: 2018-07-16T12:58:00 * Date/Time End: 2018-10-04T19:18:00
Minimum DEPTH, water: 4.6 m * Maximum DEPTH, water: 112.1 m
Event(s):
MSM77_12-1 * Latitude: 79.130260 * Longitude: 4.902700 * Date/Time: 2018-09-20T07:11:00 * Elevation: -1510.0 m * Location: Greenland Sea * Campaign: MSM77 * Basis: Maria S. Merian * Method/Device: CTD/Rosette (CTD-RO)
MSM77_13-1 * Latitude: 79.133370 * Longitude: 6.092440 * Date/Time: 2018-09-20T16:11:00 * Elevation: -1241.0 m * Location: Greenland Sea * Campaign: MSM77 * Basis: Maria S. Merian * Method/Device: CTD/Rosette (CTD-RO)
MSM77_17-1 * Latitude: 79.029890 * Longitude: 6.994430 * Date/Time: 2018-09-21T22:31:00 * Elevation: -1266.0 m * Location: Greenland Sea * Campaign: MSM77 * Basis: Maria S. Merian * Method/Device: CTD/Rosette (CTD-RO)
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Event labelEventvon Jackowski, Anabel
2Station labelStationvon Jackowski, AnabelStationbook
3Station labelStationvon Jackowski, AnabelHausgarten
4Date/Time of eventDate/Timevon Jackowski, Anabel
5LATITUDELatitudevon Jackowski, AnabelGeocode
6LONGITUDELongitudevon Jackowski, AnabelGeocode
7DEPTH, waterDepth watermvon Jackowski, AnabelGeocode
8Chlorophyll aChl aµg/lNöthig, Eva-MariaAcetone extraction (Turner Designs)
9Carbon, organic, dissolvedDOCµmol/lvon Jackowski, AnabelHigh temperature catalytic oxidation (TOC-VCSH)
10Nitrogen, total dissolvedTDNµmol/lvon Jackowski, AnabelHigh temperature catalytic oxidation (TOC-VCSH)
11Phosphorus, total dissolvedTDPµmol/lvon Jackowski, AnabelAcidic molybdate solution
12Phosphorus, organic, dissolvedDOPµmol/lvon Jackowski, AnabelAcidic molybdate solution
13Carbohydrates, dissolved combinedDCCHOnmol/lvon Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
14FucoseFuc%von Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
15RhamnoseRha%von Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
16GalactosamineGalN%von Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
17ArabinoseAra%von Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
18GlucosamineGlcN%von Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
19GalactoseGal%von Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
20GlucoseGlc%von Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
21Mannose/XyloseMan/Xyl%von Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
22Galacturonic acidGalA%von Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
23Glucuronic acidGlcA%von Jackowski, AnabelHigh performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
24Amino acid, total hydrolysable dissolvedTHDAAnmol/lvon Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
25Aspartic acidAsp%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
26Glutamic acidGlu%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
27SerineSer%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
28GlycineGly%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
29ThreonineThr%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
30ArginineArg%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
31AlanineAla%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
32TyrosineTyr%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
33ValineVal%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
34IsoleucineIle%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
35PhenylalaninePhe%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
36LeucineLeu%von Jackowski, AnabelHigh Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
37BacteriaBact#/mlvon Jackowski, AnabelFACSCalibur flow-cytometer (Becton Dicinson)
38Bacteria, heterotrophic with relatively high DNA contentHNA het#/mlvon Jackowski, AnabelFACSCalibur flow-cytometer (Becton Dicinson)
39Bacteria, heterotrophic with relatively low DNA contentLNA#/mlvon Jackowski, AnabelFACSCalibur flow-cytometer (Becton Dicinson)
40Leucine incorporation rateLeuc inc ratenmol/l/hGrosse, Julia3H-leucine microcentrifuge
41Bacterial productionBPµg/l/dayGrosse, Julia3H-leucine microcentrifugeof carbon
42Treatment: temperatureTreat temp°CGrosse, Julia3H-leucine microcentrifugeBacterial Production Incubation Temperature
43Transparent exopolymer particlesTEP#/mlvon Jackowski, AnabelMicroscopy, 20x magnification (Zeiss Axio Scope.A1)abundance
44Transparent exopolymer particlesTEPµm2/mlvon Jackowski, AnabelMicroscopy, 20x magnification (Zeiss Axio Scope.A1)total area
45Transparent exopolymer particles per carbon biomassTEP Cµg/lvon Jackowski, AnabelMicroscopy, 20x magnification (Zeiss Axio Scope.A1)
46Coomassie stainable particlesCSP#/mlvon Jackowski, AnabelMicroscopy, 20x magnification (Zeiss Axio Scope.A1)abundance
47Coomassie stainable particlesCSPµm2/mlvon Jackowski, AnabelMicroscopy, 20x magnification (Zeiss Axio Scope.A1)total area
Size:
6627 data points

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