Citation:

PANGAEA.915751

Name: Organic matter and bacteria measurements of POLARSTERN cruise PS114 and Maria S. Merian cruise MSM77
Published: 2020-04-29
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Arctic; Bacteria; CSP; Greenland Sea; organic carbon; TEP

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Abstract:

The discrete biogeochemical measurements from RV Polarstern PS114 and RV Maria S. Merian MSM77 sampled for dissolved organic matter, heterotrophic bacteria and gel particles. The parameters include chlorophyll, DOC/TDN/TDP/DOP, dissolved hydrolysable amino acids, dissolved combined carbohydrates, heterotrophic bacterial abundance and production, gel particles like TEP and CSP. The samples were aquired within 0-100 m of the Greenland Sea between 78°N to 80°N and 2°W to 14°E. The data was collected daily on board PS114 from 16th July 2018-23rd July 2018 and on board MSM77 from 16th September 2018-4th October 2018. The water collection occured using a CTD and laboratory methods varied by parameter. Chlorophyll was extracted using acetone, DOC/TDN was analysed using High-Temperature Catalytic Oxidation (TOC-VCSH), TDP/DOP was analysed colorimetrically using Acidic Molybdate Solution, dissolved hydrolysable amino acids were analysed using High Performal Liquid Chromatography (HPLC) Ortho-phthaldialdehyde Derivatization, dissolved combined carbohydrates were analysed using High Performance Anion Exchange Chromatography (HPAEC) coupled with Pulsed Amperometric Detection (PAD), cell abundance was analysed using flow cytometery, bacterial production was analysed using radioactively labelled 3H-Leucine and apllication of the microcentrifuge method, gel particles were analysed microscopically followed by image analysis. The purpose of data collection was to understand seasonal cycling of organic mater and heterotrophic bacteria dynamics within microbial loop.

Keyword(s):

Arctic; Bacteria; CSP; Greenland Sea; organic carbon; TEP

Supplement to:

von Jackowski, Anabel; Grosse, Julia; Nöthig, Eva-Maria; Engel, Anja (2020): Dynamics of organic matter and bacterial activity in the Fram Strait during summer and autumn. Philosophical Transactions of the Royal Society A-Mathematical Physical and Engineering Sciences, 378(2181), 20190366, https://doi.org/10.1098/rsta.2019.0366

Related to:

von Appen, Wilken-Jon; Schaffer, Janin; Rohardt, Gerd; Wisotzki, Andreas (2019): Physical oceanography measured on water bottle samples during POLARSTERN cruise PS114. Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven, PANGAEA, https://doi.org/10.1594/PANGAEA.898695

von Jackowski, Anabel; Engel, Anja (2019): Physical oceanography measured on water bottle samples during Maria S. Merian cruise MSM77. PANGAEA, https://doi.org/10.1594/PANGAEA.907467

Project(s):

FRontiers in Arctic marine Monitoring (FRAM)

Long-term Investigation at AWI-Hausgarten off Svalbard (Hausgarten)

Understanding the links between pelagic microbial ecosystems and organic matter cycling in the changing Arctic (μARC) (Micro-ARC)

Coverage:

Median Latitude: 79.123042 * Median Longitude: 4.708270 * South-bound Latitude: 78.187393 * West-bound Longitude: -1.836833 * North-bound Latitude: 80.300000 * East-bound Longitude: 13.999000

Date/Time Start: 2018-07-16T12:58:00 * Date/Time End: 2018-10-04T19:18:00

Minimum DEPTH, water: 4.6 m * Maximum DEPTH, water: 112.1 m

Event(s):

MSM77_3-1 * Latitude: 78.616470 * Longitude: 5.068010 * Date/Time: 2018-09-16T21:05:00 * Elevation: -2292.0 m * Location: Greenland Sea * Campaign: * Basis: * Method/Device: (CTD-RO)

MSM77_4-3 * Latitude: 79.059190 * Longitude: 4.200210 * Date/Time: 2018-09-17T17:43:00 * Elevation: -2431.0 m * Location: Greenland Sea * Campaign: * Basis: * Method/Device: (CTD-RO)

MSM77_6-1 * Latitude: 79.060020 * Longitude: 3.582410 * Date/Time: 2018-09-18T09:23:00 * Elevation: -3468.0 m * Location: Greenland Sea * Campaign: * Basis: * Method/Device: (CTD-RO)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		von Jackowski, Anabel
2	Station label	Station		von Jackowski, Anabel		Stationbook
3	Station label	Station		von Jackowski, Anabel		Hausgarten
4	Date/Time of event	Date/Time		von Jackowski, Anabel
5	LATITUDE	Latitude		von Jackowski, Anabel		Geocode
6	LONGITUDE	Longitude		von Jackowski, Anabel		Geocode
7	DEPTH, water	Depth water	m	von Jackowski, Anabel		Geocode
8	Chlorophyll a	Chl a	µg/l	Nöthig, Eva-Maria	Acetone extraction (Turner Designs)
9	Carbon, organic, dissolved	DOC	µmol/l	von Jackowski, Anabel	High temperature catalytic oxidation (TOC-VCSH)
10	Nitrogen, total dissolved	TDN	µmol/l	von Jackowski, Anabel	High temperature catalytic oxidation (TOC-VCSH)
11	Phosphorus, total dissolved	TDP	µmol/l	von Jackowski, Anabel	Acidic molybdate solution
12	Phosphorus, organic, dissolved	DOP	µmol/l	von Jackowski, Anabel	Acidic molybdate solution
13	Carbohydrates, dissolved combined	DCCHO	nmol/l	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
14	Fucose	Fuc	%	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
15	Rhamnose	Rha	%	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
16	Galactosamine	GalN	%	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
17	Arabinose	Ara	%	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
18	Glucosamine	GlcN	%	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
19	Galactose	Gal	%	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
20	Glucose	Glc	%	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
21	Mannose/Xylose	Man/Xyl	%	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
22	Galacturonic acid	GalA	%	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
23	Glucuronic acid	GlcA	%	von Jackowski, Anabel	High performance anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD), Dionex 3000
24	Amino acid, total hydrolysable dissolved	THDAA	nmol/l	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
25	Aspartic acid	Asp	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
26	Glutamic acid	Glu	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
27	Serine	Ser	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
28	Glycine	Gly	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
29	Threonine	Thr	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
30	Arginine	Arg	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
31	Alanine	Ala	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
32	Tyrosine	Tyr	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
33	Valine	Val	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
34	Isoleucine	Ile	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
35	Phenylalanine	Phe	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
36	Leucine	Leu	%	von Jackowski, Anabel	High Performance Liquid Chromatography (HPLC) ortho-phthaldialdehyde derivatization, Agilent 1260
37	Bacteria	Bact	#/ml	von Jackowski, Anabel	FACSCalibur flow-cytometer (Becton Dickinson)
38	Bacteria, heterotrophic with relatively high DNA content	HNA het	#/ml	von Jackowski, Anabel	FACSCalibur flow-cytometer (Becton Dickinson)
39	Bacteria, heterotrophic with relatively low DNA content	LNA	#/ml	von Jackowski, Anabel	FACSCalibur flow-cytometer (Becton Dickinson)
40	Leucine incorporation rate	Leuc inc rate	nmol/l/h	Grosse, Julia	3H-leucine microcentrifuge
41	Bacterial production	BP	µg/l/day	Grosse, Julia	3H-leucine microcentrifuge	of carbon
42	Treatment: temperature	T:temp	°C	Grosse, Julia	3H-leucine microcentrifuge	Bacterial Production Incubation Temperature
43	Transparent exopolymer particles	TEP	#/ml	von Jackowski, Anabel	Microscopy, 20x magnification (Zeiss Axio Scope.A1)	abundance
44	Transparent exopolymer particles	TEP	µm²/ml	von Jackowski, Anabel	Microscopy, 20x magnification (Zeiss Axio Scope.A1)	total area
45	Transparent exopolymer particles per carbon biomass	TEP C	µg/l	von Jackowski, Anabel	Microscopy, 20x magnification (Zeiss Axio Scope.A1)
46	Coomassie stainable particles	CSP	#/ml	von Jackowski, Anabel	Microscopy, 20x magnification (Zeiss Axio Scope.A1)	abundance
47	Coomassie stainable particles	CSP	µm²/ml	von Jackowski, Anabel	Microscopy, 20x magnification (Zeiss Axio Scope.A1)	total area

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

6627 data points

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