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Probst, Alexander J; Elling, Felix J; Castelle, C J; Zhu, Qing-Zeng; Elvert, Marcus; Birarda, Giovanni; Holman, Hoi-Ying N; Lane, K R; Ladd, Bethany; Ryan, M Cathryn; Woyke, Tanja; Hinrichs, Kai-Uwe; Banfield, Jillian F (2020): Intact polar lipid composition, compound-specific carbon isotopic composition, and hydrological data from Crystal Geyser, Utah, USA [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.914925

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Published: 2020-04-20 • DOI registered: 2020-06-03

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Abstract:
Sediment-hosted CO2-rich aquifers deep below the Colorado Plateau (USA) contain a remarkable diversity of uncultivated microorganisms, including Candidate Phyla Radiation (CPR) bacteria that are putative symbionts unable to synthesize membrane lipids. The origin of organic carbon in these ecosystems is unknown and the source of CPR membrane lipids remains elusive. We collected cells from deep groundwater brought to the surface by eruptions of Crystal Geyser, sequenced the community, and analyzed the whole community lipidome over time. Characteristic stable carbon isotopic compositions of microbial lipids suggest that bacterial and archaeal CO2 fixation ongoing in the deep subsurface provides organic carbon for the complex communities that reside there. Coupled lipidomic-metagenomic analysis indicates that CPR bacteria lack complete lipid biosynthesis pathways but still possess regular lipid membranes. These lipids may therefore originate from other community members, which also adapt to high in situ pressure by increasing fatty acid unsaturation. An unusually high abundance of lysolipids attributed to CPR bacteria may represent an adaptation to membrane curvature stress induced by their small cell sizes. Our findings provide new insights into the carbon cycle in the deep subsurface and suggest the redistribution of lipids into putative symbionts within this community.
Supplement to:
Probst, Alexander J; Elling, Felix J; Castelle, C J; Zhu, Qing-Zeng; Elvert, Marcus; Birarda, Giovanni; Holman, Hoi-Ying N; Lane, K R; Ladd, Bethany; Ryan, M Cathryn; Woyke, Tanja; Hinrichs, Kai-Uwe; Banfield, Jillian F (2020): Lipid analysis of CO2-rich subsurface aquifers suggests an autotrophy-based deep biosphere with lysolipids enriched in CPR bacteria. The ISME Journal, https://doi.org/10.1038/s41396-020-0624-4
Coverage:
Latitude: 38.938361 * Longitude: -110.135389
Date/Time Start: 2015-05-24T15:08:00 * Date/Time End: 2015-05-29T19:30:00
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
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19 datasets

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Datasets listed in this publication series

  1. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG03. https://doi.org/10.1594/PANGAEA.914891
  2. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG05. https://doi.org/10.1594/PANGAEA.914892
  3. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG07. https://doi.org/10.1594/PANGAEA.914893
  4. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG09. https://doi.org/10.1594/PANGAEA.914894
  5. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG11. https://doi.org/10.1594/PANGAEA.914895
  6. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG13. https://doi.org/10.1594/PANGAEA.914896
  7. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG15. https://doi.org/10.1594/PANGAEA.914897
  8. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG17. https://doi.org/10.1594/PANGAEA.914898
  9. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG19. https://doi.org/10.1594/PANGAEA.914899
  10. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG21. https://doi.org/10.1594/PANGAEA.914900
  11. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG23. https://doi.org/10.1594/PANGAEA.914901
  12. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG24. https://doi.org/10.1594/PANGAEA.914902
  13. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG25. https://doi.org/10.1594/PANGAEA.914903
  14. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations, fractional abundance, and characteristics of intact polar lipids in lipid extracts obtained by filtering eruption fluids from Crystal Geyser, sample CG29. https://doi.org/10.1594/PANGAEA.914904
  15. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Carbon isotopic composition of dissolved inorganic carbon and IPL-derived fatty acids and phytane in lipid extracts obtained by filtering eruption fluids from Crystal Geyser. https://doi.org/10.1594/PANGAEA.914989
  16. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Concentrations of IPL-derived fatty acids and phytane in lipid extracts obtained by filtering eruption fluids from Crystal Geyser. https://doi.org/10.1594/PANGAEA.914988
  17. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Sampling information from Crystal Geyser, Utah, USA. https://doi.org/10.1594/PANGAEA.914865
  18. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Hydrological data from Crystal Geyser, Utah, USA, determined with levelogger 1. https://doi.org/10.1594/PANGAEA.914863
  19. Probst, AJ; Elling, FJ; Castelle, CJ et al. (2020): Hydrological data from Crystal Geyser, Utah, USA, determined with levelogger 2. https://doi.org/10.1594/PANGAEA.914864