Citation:

PANGAEA.914653

Name: Seawater carbonate chemistry and hypoxia tolerance and blood chemistry characteristics of European sea bass
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Acid-base regulation; Animalia; Brackish waters; Chordata; Containers and aquaria (20-1000 L or < 1 m**2); Dicentrarchus labrax; Laboratory experiment; Nekton; North Atlantic; Other studied parameter or process; Oxygen; Pelagos; Respiration; Single species

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Abstract:

Global environmental change is increasing hypoxia in aquatic ecosystems. During hypoxic events, bacterial respiration causes an increase in carbon dioxide (CO2) while oxygen (O2) declines. This is rarely accounted for when assessing hypoxia tolerances of aquatic organisms. We investigated the impact of environmentally realistic increases in CO2 on responses to hypoxia in European sea bass (Dicentrarchus labrax). We conducted a critical oxygen (O2crit) test, a common measure of hypoxia tolerance, using two treatments in which O2 levels were reduced with constant ambient CO2 levels (~530 µatm), or with reciprocal increases in CO2 (rising to ~2,500 µatm). We also assessed blood acid-base chemistry and haemoglobin-O2 binding affinity of sea bass in hypoxic conditions with ambient (~650 μatm) or raised CO2 (~1770 μatm) levels. Sea bass exhibited greater hypoxia tolerance (~20% reduced O2crit), associated with increased haemoglobin-O2 affinity (~32% fall in P50) of red blood cells, when exposed to reciprocal changes in O2 and CO2. This indicates that rising CO2 which accompanies environmental hypoxia facilitates increased O2 uptake by the blood in low O2 conditions, enhancing hypoxia tolerance. We recommend that when impacts of hypoxia on aquatic organisms are assessed, due consideration is given to associated environmental increases in CO2.

Keyword(s):

Acid-base regulation; Animalia; Brackish waters; Chordata; Containers and aquaria (20-1000 L or < 1 m**2); Dicentrarchus labrax; Laboratory experiment; Nekton; North Atlantic; Other studied parameter or process; Oxygen; Pelagos; Respiration; Single species

Supplement to:

Montgomery, Daniel W; Simpson, Stephen D; Engelhard, Georg H; Birchenough, Silvana N R; Wilson, Rod W (2019): Rising CO2 enhances hypoxia tolerance in a marine fish. Scientific Reports, 9(1), https://doi.org/10.1038/s41598-019-51572-4

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-04-02.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Montgomery, Daniel W		study
2	Species	Species		Montgomery, Daniel W
3	Registration number of species	Reg spec no		Montgomery, Daniel W
4	Uniform resource locator/link to reference	URL ref		Montgomery, Daniel W		WoRMS Aphia ID
5	Treatment	Treat		Montgomery, Daniel W
6	Identification	ID		Montgomery, Daniel W		Fish
7	Mass	Mass	kg	Montgomery, Daniel W
8	Date	Date		Montgomery, Daniel W		Pcrit
9	Temperature, water	Temp	°C	Montgomery, Daniel W
10	Temperature, water, standard deviation	Temp std dev	±	Montgomery, Daniel W
11	Oxygen saturation	O2 sat	%	Montgomery, Daniel W		Critical oxygen level
12	Oxygen, partial pressure, critical	pO2 crit	kPa	Montgomery, Daniel W
13	Metabolic rate, standard	SMR	mg/kg/h	Montgomery, Daniel W
14	Covariance	Cov		Montgomery, Daniel W
15	Date	Date		Montgomery, Daniel W
16	Oxygen, partial pressure	pO2	mm Hg	Montgomery, Daniel W		Chamber
17	Oxygen, partial pressure	pO2	kPa	Montgomery, Daniel W		Chamber
18	Oxygen, partial pressure	pO2	mm Hg	Montgomery, Daniel W		Irrigation
19	Oxygen, partial pressure	pO2	kPa	Montgomery, Daniel W		Irrigation
20	Oxygen, partial pressure	pO2	mm Hg	Montgomery, Daniel W		Blood
21	Oxygen, partial pressure	pO2	kPa	Montgomery, Daniel W		Blood
22	Blood, ph	Blood pH		Montgomery, Daniel W
23	Carbon dioxide, partial pressure, blood	pCO2 blood	mm Hg	Montgomery, Daniel W
24	Blood, partial pressure of carbon dioxide	Blood pCO2	kPa	Montgomery, Daniel W
25	Blood, bicarbonate, blood	Blood [HCO3]-	mmol/l	Montgomery, Daniel W
26	Half saturation partial pressure of oxygen	p50	mm Hg	Montgomery, Daniel W
27	Half saturation partial pressure of oxygen	p50	kPa	Montgomery, Daniel W
28	Hill coefficient	Hill coeff		Montgomery, Daniel W
29	Haematocrit	Ht	%	Montgomery, Daniel W
30	Salinity	Sal		Montgomery, Daniel W
31	Salinity, standard deviation	Sal std dev	±	Montgomery, Daniel W
32	pH	pH		Montgomery, Daniel W		NBS scale
33	pH, standard deviation	pH std dev	±	Montgomery, Daniel W		NBS scale
34	Oxygen saturation	O2 sat	%	Montgomery, Daniel W
35	Oxygen saturation, standard deviation	O2 sat std dev	±	Montgomery, Daniel W
36	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Montgomery, Daniel W
37	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Montgomery, Daniel W
38	Alkalinity, total	AT	µmol/kg	Montgomery, Daniel W
39	Alkalinity, total, standard deviation	AT std dev	±	Montgomery, Daniel W
40	Time in hours	Time	h	Montgomery, Daniel W
41	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
43	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1082 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 ID (Fish)	7 Mass [kg]	8 Date (Pcrit)	9 Temp [°C]	10 Temp std dev [±]	11 O2 sat [%] (Critical oxygen level)	12 pO2 crit [kPa]	13 SMR [mg/kg/h]	14 Cov	15 Date	16 pO2 [mm Hg] (Chamber)	17 pO2 [kPa] (Chamber)	18 pO2 [mm Hg] (Irrigation)	19 pO2 [kPa] (Irrigation)	20 pO2 [mm Hg] (Blood)	21 pO2 [kPa] (Blood)	22 Blood pH	23 pCO2 blood [mm Hg]	24 Blood pCO2 [kPa]	25 Blood [HCO3]- [mmol/l]	26 p50 [mm Hg]	27 p50 [kPa]	28 Hill coeff	29 Ht [%]	30 Sal	31 Sal std dev [±]	32 pH (NBS scale)	33 pH std dev [±] (NBS scale)	34 O2 sat [%]	35 O2 sat std dev [±]	36 pCO2water_SST_wet [µatm]	37 pCO2 std dev [±]	38 AT [µmol/kg]	39 AT std dev [±]	40 Time [h]	41 CSC flag	42 pH (total scale)	43 CO2 [µmol/kg]	44 fCO2water_SST_wet [µatm]	45 pCO2water_SST_wet [µatm]	46 [HCO3]- [µmol/kg]	47 [CO3]2- [µmol/kg]	48 DIC [µmol/kg]	49 Omega Arg	50 Omega Cal
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	1	0.104	2018-03-28T00:00	18.30		25.5	5.29820	125.4	8.33
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	2	0.166	2018-03-28T00:00	18.30		19.3	4.01001	98.1	3.80
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	3	0.110	2018-03-28T00:00	18.30		24.1	5.00732	117.1	9.60
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	4	0.090	2018-03-28T00:00	18.30		24.0	4.98654	118.8	14.95
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	5	0.099	2018-03-28T00:00	18.30		22.9	4.75799	133.7	6.78
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	6	0.170	2018-04-04T00:00	18.20		18.7	3.88586	83.2	7.94
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	7	0.131	2018-04-04T00:00	18.20		26.3	5.46514	114.1	7.02
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	8	0.102	2018-04-04T00:00	18.20		26.6	5.52748	117.4	13.60
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	9	0.101	2018-04-17T00:00	18.20		15.2	3.15856	100.3	20.90
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	10	0.147	2018-04-17T00:00	18.20		17.1	3.55338	85.4	19.30
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	11	0.135	2018-04-17T00:00	18.20		19.5	4.05210	113.1	14.00
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	12	0.152	2018-04-19T00:00	18.35		18.2	3.78121	97.6	13.03
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	13	0.165	2018-04-19T00:00	18.35		18.9	3.92664	88.3	14.00
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	14	0.180	2018-04-19T00:00	18.35		18.5	3.84354	99.7	10.60
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	15	0.143	2018-04-19T00:00	18.35		23.3	4.84078	124.4	12.60
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	16*	0.104	2018-04-19T00:00	18.35		24.7	5.13164	122.1	33.20
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	1	0.104		17.60						2018-03-29T00:00	53.90	7.19	57.90	7.72	15.00	1.99	7.895	1.19	0.159	4.14	21.10	2.810	1.410	40.2	32.150		8.02		34.50		548.00		2071.2			26	7.90	19.20	545.08	546.99	1799.49	108.82	1927.52	1.70	2.65
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	2	0.166		17.70						2018-03-29T00:00	60.20	8.02	56.80	7.57	13.60	1.81	7.920	0.98	0.131	3.65	19.40	2.592	1.187	42.5	32.150		7.98		38.60		608.00		2070.1			26	7.86	21.24	604.50	606.62	1818.73	100.68	1940.65	1.58	2.45
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	3	0.110		17.50						2018-03-29T00:00	63.10	8.41	66.20	8.82	42.60	5.68	7.870	0.66	0.088	2.17	13.90	1.859	1.417	39.7	32.150		8.08		40.40		463.00		2050.3			26	7.96	16.27	460.50	462.12	1747.63	120.95	1884.85	1.89	2.94
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	4	0.090		17.40						2018-03-29T00:00	64.30	8.57	66.10	8.81	7.00	0.93	7.920	1.38	0.184	5.13	16.80	2.240	1.147	40.5	32.710		7.94		41.20		765.10		2363.3			26	7.82	26.87	760.62	763.30	2102.85	106.76	2236.48	1.66	2.59
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	5	0.099		17.20						2018-03-29T00:00	64.80	8.64	70.00	9.33	17.70	2.36	7.830	1.60	0.213	4.72	12.70	1.690	1.295	39.0	32.710		7.91		41.50		726.30		2086.9			26	7.79	25.67	722.23	724.78	1867.26	87.86	1980.79	1.37	2.13
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	6	0.170		17.10						2018-04-05T00:00	58.30	7.77	61.70	8.23			7.960							22.0
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	7	0.131		17.00						2018-04-05T00:00	63.30	8.44	66.40	8.85	41.10	5.48	7.800	0.97	0.129	2.65	21.00	2.800	1.142	38.7	32.710		7.90		40.60		757.80		2123.7			26	7.78	26.86	753.57	756.24	1906.08	87.36	2020.29	1.36	2.12
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with fixed CO2	8	0.102		17.10						2018-04-05T00:00	57.50	7.67	66.30	8.84	36.20	4.83	7.730	1.20	0.160	2.74	22.80	3.040	1.169	35.3	32.710		7.92		36.80		723.20		2134.0			26	7.80	25.71	719.23	721.77	1906.98	91.20	2023.89	1.42	2.21
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	9	0.101		17.90						2018-04-18T00:00	64.60	8.61	63.90	8.52	42.50	5.67	7.915	1.34	0.178	4.83	13.58	1.810	1.570	42.7	33.030		7.58		41.40		1713.80		2164.4			26	7.46	59.29	1705.78	1711.75	2048.98	46.52	2154.79	0.73	1.13
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	10	0.147		17.90						2018-04-18T00:00	66.75	8.90	65.00	8.67	39.95	4.39	7.920	1.91	0.255	7.11	12.23	1.630	1.383	36.3	33.030		7.58		42.80		1792.70		2262.9			26	7.46	62.03	1784.54	1790.78	2143.22	48.65	2253.90	0.76	1.18
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	11	0.135		18.00						2018-04-18T00:00	60.90	8.12	64.10	8.55	29.80	3.97	7.950	1.68	0.224	6.72	13.65	1.820	1.700	39.3	33.030		7.55		39.10		1997.30		2341.5			26	7.43	68.91	1988.24	1995.20	2225.88	47.31	2342.10	0.74	1.15
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	12	0.152		17.80						2018-04-20T00:00	71.40	9.52	61.60	8.21	25.65	3.42	7.845	2.31	0.308	7.09				33.3	33.030		7.59		45.80		1736.30		2247.9			26	7.47	60.22	1727.47	1733.53	2126.55	49.26	2236.03	0.77	1.19
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	13	0.165		17.70						2018-04-20T00:00	62.10	8.28	69.10	9.21	43.60	5.81	7.860	2.16	0.288	6.89	9.68	1.290	1.160	41.0	33.030		7.60		39.80		1700.10		2257.3			26	7.48	59.15	1691.92	1697.85	2133.25	50.39	2242.79	0.79	1.22
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	14	0.180		17.70						2018-04-20T00:00	57.85	7.71	69.10	9.21	22.35	2.98	7.805	2.59	0.345	7.17	11.70	1.560	1.135	40.3	33.030		7.56		37.10		1893.10		2281.0			26	7.44	65.87	1884.12	1890.73	2166.36	46.66	2278.89	0.73	1.13
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	15	0.143		17.80						2018-04-20T00:00	66.95	8.93	67.00	8.93	27.15	3.62	7.880	2.06	0.274	6.90	17.25	2.300	1.186	37.5	33.030		7.62		42.90		1645.00		2290.4			26	7.50	57.06	1636.74	1642.48	2158.86	53.58	2269.50	0.84	1.30
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	Hypoxia with increasing CO2	16*	0.104		17.70						2018-04-20T00:00	68.65	9.15	61.95	8.26	33.75	4.50	7.870	2.26	0.301	7.39	8.18	1.090	1.124	35.3	33.030		7.62		44.00		1674.50		2333.3			26	7.50	58.27	1666.55	1672.40	2200.16	54.41	2312.84	0.85	1.32
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with Rising CO2				17.80	0.42																				33.205	0.247487373	8.04	0.057	95.57	1.08	555.30	102.4	2216.5	73.8	0	26	7.92	19.11	548.82	550.74	1907.03	125.13	2051.28	1.95	3.03
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with Rising CO2				17.80	0.49																				33.205	0.247487373	8.06	0.028	75.03	1.05	527.30	66.6	2224.9	100.1	1	26	7.94	18.23	523.33	525.16	1902.23	130.57	2051.02	2.03	3.16
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with Rising CO2				17.70	0.49																				33.205	0.247487373	8.00	0.071	57.96	0.98	617.60	141.4	2209.8	89.2	2	26	7.88	21.23	607.81	609.94	1925.89	114.72	2061.84	1.79	2.77
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with Rising CO2				17.80	0.42																				33.205	0.247487373	7.92	0.071	38.27	0.57	761.30	176.0	2215.9	98.8	3	26	7.80	26.07	748.45	751.07	1973.02	98.22	2097.30	1.53	2.37
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with Rising CO2				17.90	0.35																				33.205	0.247487373	7.80	0.049	28.97	0.77	1030.50	175.4	2198.6	89.5	4	26	7.67	35.44	1020.52	1024.09	2012.25	75.26	2122.95	1.17	1.82
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with Rising CO2				17.90	0.35																				33.205	0.247487373	7.63	0.049	20.60	1.12	1559.90	252.5	2188.6	78.7	5	26	7.50	53.68	1545.75	1551.16	2059.63	52.05	2165.36	0.81	1.26
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with Rising CO2				17.90	0.28																				33.205	0.247487373	7.45	0.049	16.55	1.12	2393.10	383.5	2174.2	79.4	6	26	7.32	82.27	2368.93	2377.23	2087.67	34.90	2204.83	0.54	0.84
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with constant ambient CO2				17.90	0.42																				32.365	0.289913780	8.01	0.042	94.73	1.89	537.05	37.7	1974.4	70.5	0	26	7.89	18.58	532.43	534.29	1714.62	102.96	1836.15	1.61	2.50
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with constant ambient CO2																													74.69	1.78					1
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with constant ambient CO2																													57.71	3.37					2
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with constant ambient CO2																													37.85	3.80					3
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with constant ambient CO2																													29.96	2.23					4
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with constant ambient CO2																													23.29	1.74					5
laboratory	Dicentrarchus labrax (fish)	126975	marinespecies.org	O2crit trial with constant ambient CO2				18.30	0.07																				32.975	0.572756493	8.03	0.064	16.65	1.46	523.40	57.0	2044.4	169.5	6	26	7.90	18.20	529.47	531.31	1761.66	112.64	1892.50	1.76	2.73