Citation:

PANGAEA.913498

Name: Seawater carbonate chemistry and growth, production rates, and cellular composition of Arctic diatom
Published: 2019
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Arctic; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Ochrophyta; Pelagos; Phytoplankton; Polar; Primary production/Photosynthesis; Single species; Temperature; Thalassiosira hyalina

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Abstract:

Arctic phytoplankton and their response to future conditions shape one of the most rapidly changing ecosystems on the planet. We tested how much the phenotypic responses of strains from the same Arctic diatom population diverge and whether the physiology and intraspecific composition of multistrain populations differs from expectations based on single strain traits. To this end, we conducted incubation experiments with the diatom Thalassiosira hyalina under present‐day and future temperature and pCO2 treatments. Six fresh isolates from the same Svalbard population were incubated as mono‐ and multistrain cultures. For the first time, we were able to closely follow intraspecific selection within an artificial population using microsatellites and allele‐specific quantitative PCR. Our results showed not only that there is substantial variation in how strains of the same species cope with the tested environments but also that changes in genotype composition, production rates, and cellular quotas in the multistrain cultures are not predictable from monoculture performance. Nevertheless, the physiological responses as well as strain composition of the artificial populations were highly reproducible within each environment. Interestingly, we only detected significant strain sorting in those populations exposed to the future treatment. This study illustrates that the genetic composition of populations can change on very short timescales through selection from the intraspecific standing stock, indicating the potential for rapid population level adaptation to climate change. We further show that individuals adjust their phenotype not only in response to their physicochemical but also to their biological surroundings. Such intraspecific interactions need to be understood in order to realistically predict ecosystem responses to global change.

Keyword(s):

Arctic; Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Coast and continental shelf; Growth/Morphology; Laboratory experiment; Ochrophyta; Pelagos; Phytoplankton; Polar; Primary production/Photosynthesis; Single species; Temperature; Thalassiosira hyalina

Supplement to:

Wolf, Klara K E; Romanelli, Elisa; Rost, Björn; John, Uwe; Collins, Sinéad; Weigand, Hannah; Hoppe, Clara Jule Marie (2019): Company matters: The presence of other genotypes alters traits and intraspecific selection in an Arctic diatom under climate change. Global Change Biology, 25(9), 2869-2884, https://doi.org/10.1111/gcb.14675

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 78.916670 * Longitude: 11.933330

Event(s):

KongsfjordenOA * Latitude: 78.916670 * Longitude: 11.933330 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-03-06.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Wolf, Klara K E		study
2	Species	Species		Wolf, Klara K E
3	Registration number of species	Reg spec no		Wolf, Klara K E
4	Uniform resource locator/link to reference	URL ref		Wolf, Klara K E		WoRMS Aphia ID
5	Strain	Strain		Wolf, Klara K E		culture
6	Treatment	Treat		Wolf, Klara K E
7	Growth rate	µ	1/day	Wolf, Klara K E
8	Growth rate, standard deviation	µ std dev	±	Wolf, Klara K E
9	Contribution	Contribution	%	Wolf, Klara K E
10	Contribution, standard deviation	Contribution std dev	±	Wolf, Klara K E
11	Bulk division rate	k	1/day	Wolf, Klara K E
12	Bulk division rate, standard deviation	k std dev	±	Wolf, Klara K E
13	Carbon, organic, particulate, per cell	POC/cell	pg/#	Wolf, Klara K E
14	Carbon, organic, particulate, standard deviation	POC std dev	±	Wolf, Klara K E
15	Carbon, organic, particulate, production per cell	POC prod/cell	pg/#/day	Wolf, Klara K E
16	Particulate organic carbon, production, standard deviation	POC prod std dev	±	Wolf, Klara K E
17	Chlorophyll a per cell	Chl a/cell	pg/#	Wolf, Klara K E
18	Chlorophyll a, standard deviation	Chl a std dev	±	Wolf, Klara K E
19	Carbon/Nitrogen ratio	C/N	mol/mol	Wolf, Klara K E
20	Carbon/Nitrogen ratio, standard deviation	C/N std dev	±	Wolf, Klara K E
21	Carbon, organic, particulate/chlorophyll a ratio	POC/Chl a	g/g	Wolf, Klara K E
22	Carbon, organic, particulate/chlorophyll a ratio, standard deviation	POC/Chl a std dev	±	Wolf, Klara K E
23	Maximum light use efficiency	alpha	mol e m²/mol RCII/mol photons	Wolf, Klara K E
24	Maximum light utilization coefficient in carbon per chlorophyll a, standard deviation	alpha std dev	±	Wolf, Klara K E
25	Maximal absolute electron transfer rate	ETR max	mol e/mol RCII/s	Wolf, Klara K E
26	Maximal electron transport rate, standard deviation	ETR max std dev	±	Wolf, Klara K E
27	Irradiance	E	µmol/m²/s	Wolf, Klara K E		In situ ETR, the growth conditions
28	Irradiance, standard deviation	E std dev	±	Wolf, Klara K E		In situ ETR, the growth conditions
29	Salinity	Sal		Wolf, Klara K E
30	Temperature, water	Temp	°C	Wolf, Klara K E
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Wolf, Klara K E	Calculated using CO2SYS
32	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Wolf, Klara K E	Calculated using CO2SYS
33	pH	pH		Wolf, Klara K E	Potentiometric	NBS scale
34	pH, standard deviation	pH std dev	±	Wolf, Klara K E	Potentiometric	NBS scale
35	Alkalinity, total	AT	µmol/kg	Wolf, Klara K E	Potentiometric titration
36	Alkalinity, total, standard deviation	AT std dev	±	Wolf, Klara K E	Potentiometric titration
37	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
39	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

939 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Strain (culture)	6 Treat	7 µ [1/day]	8 µ std dev [±]	9 Contribution [%]	10 Contribution std dev [±]	11 k [1/day]	12 k std dev [±]	13 POC/cell [pg/#]	14 POC std dev [±]	15 POC prod/cell [pg/#/day]	16 POC prod std dev [±]	17 Chl a/cell [pg/#]	18 Chl a std dev [±]	19 C/N [mol/mol]	20 C/N std dev [±]	21 POC/Chl a [g/g]	22 POC/Chl a std dev [±]	23 alpha [mol e m²/mol RCII/mol photons]	24 alpha std dev [±]	25 ETR max [mol e/mol RCII/s]	26 ETR max std dev [±]	27 E [µmol/m²/s] (In situ ETR, the growth condi...)	28 E std dev [±] (In situ ETR, the growth condi...)	29 Sal	30 Temp [°C]	31 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	32 pCO2 std dev [±] (Calculated using CO2SYS)	33 pH (NBS scale, Potentiometric)	34 pH std dev [±] (NBS scale, Potentiometric)	35 AT [µmol/kg] (Potentiometric titration)	36 AT std dev [±] (Potentiometric titration)	37 CSC flag (Calculated using seacarb afte...)	38 pH (total scale, Calculated using...)	39 CO2 [µmol/kg] (Calculated using seacarb afte...)	40 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	41 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	42 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	43 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	44 DIC [µmol/kg] (Calculated using seacarb afte...)	45 Omega Arg (Calculated using seacarb afte...)	46 Omega Cal (Calculated using seacarb afte...)
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain A	present	0.76	0.010	22	2	1.10	0.02	300	14	330	9	10.7	0.2	4.9	0.04	28.0	1.7	1.37	0.04	61.5	6.8	45.0	4.3	32	2	378	11	8.14	0.01	2212	18	15	8.04	22.48	379.21	380.84	1975.62	90.50	2088.60	1.37	2.20
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain A	future	0.74	0.010	7	2	1.06	0.01	246	5	262	3	10.6	1.2	4.8	0.01	23.4	2.2	1.28	0.07	72.1	3.4	56.6	5.4	32	7	1191	25	7.70	0.01	2232	16	15	7.60	58.69	1187.34	1192.12	2118.96	42.35	2220.01	0.65	1.03
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain A	warming	0.77	0.010			1.11	0.01	252	13	279	13	9.2	0.1	4.8	0.20	27.5	1.5							32	7	389	12	8.13	0.01	2123	9	15	8.03	19.17	387.78	389.34	1861.75	100.11	1981.03	1.53	2.43
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain B	present	0.78	0.030	21	1	1.13	0.04	241	8	273	15	7.9	0.3	5.5	0.70	30.6	2.0	1.41	0.04	60.9	3.5	43.3	3.4	32	2	377	10	8.15	0.01	2226	7	15	8.05	22.06	372.09	373.69	1983.65	92.98	2098.69	1.41	2.26
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain B	future	0.80	0.020	19	2	1.15	0.04	264	1	304	9	7.0	0.1	6.0	0.70	37.9	0.6	1.32	0.06	44.5	1.4	33.7	2.5	32	7	1249	18	7.68	0.01	2205	2	15	7.58	60.83	1230.67	1235.63	2097.54	40.04	2198.42	0.61	0.97
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain B	warming	0.74	0.010			1.07	0.01	190	14	202	14	5.3	0.5	4.5	0.90	35.7	3.2							32	7	407	9	8.11	0.01	2114	6	15	8.01	20.09	406.49	408.12	1863.72	95.71	1979.52	1.46	2.32
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain C	present	0.82	0.030	13	1	1.18	0.04	241	30	285	45	6.3	0.8	4.7	0.20	38.0	0.2	1.26	0.00	57.9	3.0	45.9	2.3	32	2	339	9	8.19	0.01	2242	10	15	8.09	20.06	338.42	339.88	1978.09	101.66	2099.81	1.54	2.47
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain C	future	0.76	0.020	7	1	1.09	0.03	185	8	202	6	6.2	0.3	4.8	0.10	29.9	0.4	1.26	0.03	49.0		38.9	0.8	32	7	1229	30	7.69	0.01	2237	4	15	7.59	60.26	1219.12	1224.03	2126.10	41.53	2227.89	0.63	1.01
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain C	warming	0.75	0.030			1.08	0.04	203	4	220	5	7.4	0.2	4.6	0.40	27.5	0.3							32	7	433	5	8.09	0.00	2123	7	15	7.99	21.24	429.72	431.45	1881.66	92.28	1995.18	1.41	2.24
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain X	present	0.74	0.030	17	1	1.07	0.04	187	11	200	9	7.5	0.2	5.5	0.30	25.0	1.3	1.37	0.10	38.8	1.1	28.4	2.7	32	2	414	4	8.08	0.00	2102	2	15	7.98	24.82	418.75	420.55	1900.60	75.85	2001.27	1.15	1.84
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain X	future	0.71	0.020	16	2	1.02	0.03	164	5	167	9	6.5	0.4	4.2	1.00	25.5	2.5	1.31	0.03	52.1	2.0	39.9	2.4	32	7	1180	33	7.70	0.01	2237	10	15	7.60	58.83	1190.06	1194.85	2123.77	42.45	2225.05	0.65	1.03
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain X	warming	0.80	0.020			1.15	0.03	170	16	196	23	7.0	1.0	4.4	1.40	24.7	4.0							32	7	427	4	8.09	0.00	2114	8	15	7.99	21.15	427.85	429.57	1873.46	91.88	1986.49	1.40	2.23
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain Y	present	0.79	0.010	10	3	1.14	0.01	281	8	321	12	11.2	0.5	4.7	0.10	25.1	0.8	1.25	0.02	46.8	6.7	37.3	4.7	32	2	408	6	8.09	0.01	2121	10	15	7.99	24.43	412.13	413.90	1914.07	78.16	2016.66	1.19	1.90
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain Y	future	0.82	0.010	45	2	1.19	0.02	239	2	284	2	8.8	0.4	4.8	0.10	27.3	1.3	1.04	0.03	55.4	6.4	53.5	6.7	32	7	1152	22	7.72	0.01	2254	13	15	7.62	56.48	1142.67	1147.26	2135.29	44.69	2236.47	0.68	1.08
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain Y	warming	0.73	0.050			1.05	0.07	225	11	236	28	9.3	0.6	4.8	0.10	24.1	0.4							32	7	416	11	8.10	0.01	2116	1	15	8.00	20.63	417.44	419.12	1870.45	93.87	1984.95	1.43	2.28
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain Z	present	0.73	0.030	13	1	1.06	0.04	344	35	365	44	10.4	0.2	4.7	0.10	33.2	3.5	1.24	0.03	66.0	1.9	53.2	2.9	32	2	396	9	8.13	0.01	2238	2	15	8.03	23.33	393.62	395.32	2004.17	89.72	2117.22	1.36	2.18
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain Z	future	0.80	0.002	5	0	1.15	0.00	330	37	380	43	10.1	0.1	4.8	0.30	32.6	3.2	1.28	0.01	56.4	1.4	44.1	1.1	32	7	1191	11	7.70	0.00	2222	4	15	7.60	58.43	1181.99	1186.75	2109.35	42.16	2209.94	0.64	1.02
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Strain Z	warming	0.71	0.030			1.02	0.04	271	6	276	9	9.5	0.5	5.2	0.90	28.6	0.9							32	7	412	6	8.11	0.01	2121	9	15	8.01	20.16	407.86	409.50	1870.06	96.03	1986.26	1.47	2.33
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Mean Monocultures	present	0.77	0.030			1.11	0.05	266	55	296	58	9.0	2.0	5.0	0.40	30.0	5.1	1.32	0.07	55.3	10.3	42.2	8.5
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Mean Monocultures	future	0.75	0.030			1.08	0.05	219	38	235	36	7.9	1.7	4.7	0.30	29.4	1.1	1.25	0.11	54.9	9.5	44.5	8.9
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Mean Monocultures	warming	0.77	0.010			1.11	0.01	238	14	266	15	8.2	0.4	4.9	0.40	28.0	4.2
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Multi-Strain	present	0.73	0.020			1.06	0.02	217	5	230	1	7.1	0.2	4.9	0.10	30.6	0.7	1.29	0.04	61.1	3.9	47.6	3.0	32	2	350	7	8.18	0.01	2263	40	15	8.08	20.78	350.54	352.05	2002.36	100.57	2123.70	1.53	2.44
laboratory	Thalassiosira hyalina (phytoplankton)	149100	marinespecies.org	Multi-Strain	future	0.81	0.020			1.18	0.02	187	4	219	5	6.8	0.3	4.9	0.10	27.5	1.4	1.31	0.03	60.9	3.7	46.6	2.4	32	7	1095	65	7.74	0.03	2238	16	15	7.64	53.43	1080.86	1085.21	2114.94	46.35	2214.72	0.71	1.12