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Flores-Higuera, Francisco A; Wördenweber, Robin; Luis-Villaseñor, Irasema E; Rochin-Arenas, Jesús A; Gómez-Gil, Bruno; Mazón-Suástegui, José Manuel; Voltolina, D (2019): Seawater carbonate chemistry and the bacterial community present in larvae and spat of Crassostrea gigas [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.913332

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Abstract:
Changes in marine environments, including pH changes, have been correlated to alterations in the physiology and disease susceptibility of cultured organisms at the early stages of development. In this study, high-throughput sequencing of the V3-V4 region of the 16S rRNA gene was performed to evaluate the bacterial
biodiversity of Crassostrea gigas pediveliger larvae and spat under acidic stress compared to that of larvae at normal pH value. The evaluation was performed in an experimental system with continuous water flow and pH
manipulation by CO2 bubbling to simulate acidification (pH 7.38 ± 0.039), using the current ocean pH conditions (pH 8.116 ± 0.023) as a reference. The results indicated that the bacterial communities associated with both pediveliger larvae and spat were modified in response to acidic conditions. The families Rhodobacteraceae and Campylobacteraceae were the most affected by the change in pH, with increases in Vibrionaceae in pediveliger larvae and Planctomycetaceae and Phyllobacteriaceae in spat detected. The results of this study demonstrate that the bacterial communities associated with C. gigas pediveliger larvae and spat are responsive to changes in ocean acidification
Keyword(s):
Animalia; Bottles or small containers/Aquaria (<20 L); Community composition and diversity; Crassostrea gigas; Entire community; Laboratory experiment; Mollusca; Not applicable; Pelagos; Single species; Zooplankton
Supplement to:
Flores-Higuera, Francisco A; Luis-Villaseñor, Irasema E; Rochin-Arenas, Jesús A; Gómez-Gil, Bruno; Mazón-Suástegui, José Manuel; Voltolina, Domenico; Medina-Hernández, Diana (2019): Effect of pH on the bacterial community present in larvae and spat of Crassostrea gigas. Latin American Journal of Aquatic Research, 47(3), 513-523, https://doi.org/10.3856/vol47-issue3-fulltext-13
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-03-06.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1TypeTypeLuis-Villaseñor, Irasema Estudy
2SpeciesSpeciesLuis-Villaseñor, Irasema E
3Registration number of speciesReg spec noLuis-Villaseñor, Irasema E
4Uniform resource locator/link to referenceURL refLuis-Villaseñor, Irasema EWoRMS Aphia ID
5Life stageLife stageLuis-Villaseñor, Irasema E
6TreatmentTreatLuis-Villaseñor, Irasema E
7Experiment durationExp durationhLuis-Villaseñor, Irasema E
8Shannon Diversity IndexH'Luis-Villaseñor, Irasema E
9Shannon Diversity Index, standard deviationH' std dev±Luis-Villaseñor, Irasema E
10Evenness of speciesELuis-Villaseñor, Irasema E
11Evenness of species, standard deviationE std dev±Luis-Villaseñor, Irasema E
12Simpson index of diversitySimpson ILuis-Villaseñor, Irasema E
13Simpson index of diversity, standard deviationSimpsons I std dev±Luis-Villaseñor, Irasema E
14Temperature, waterTemp°CLuis-Villaseñor, Irasema E
15Temperature, water, standard deviationTemp std dev±Luis-Villaseñor, Irasema E
16SalinitySalLuis-Villaseñor, Irasema E
17Salinity, standard deviationSal std dev±Luis-Villaseñor, Irasema E
18pHpHLuis-Villaseñor, Irasema Etotal scale
19pH, standard deviationpH std dev±Luis-Villaseñor, Irasema Etotal scale
20Alkalinity, totalATµmol/kgLuis-Villaseñor, Irasema E
21Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmLuis-Villaseñor, Irasema E
22Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Luis-Villaseñor, Irasema E
23Bicarbonate ion[HCO3]-µmol/kgLuis-Villaseñor, Irasema E
24Bicarbonate ion, standard deviation[HCO3]- std dev±Luis-Villaseñor, Irasema E
25Carbonate ion[CO3]2-µmol/kgLuis-Villaseñor, Irasema E
26Carbonate ion, standard deviation[CO3]2- std dev±Luis-Villaseñor, Irasema E
27Calcite saturation stateOmega CalLuis-Villaseñor, Irasema E
28Calcite saturation state, standard deviationOmega Cal std dev±Luis-Villaseñor, Irasema E
29Aragonite saturation stateOmega ArgLuis-Villaseñor, Irasema E
30Aragonite saturation state, standard deviationOmega Arg std dev±Luis-Villaseñor, Irasema E
31Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
32Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
33Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
34Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
35Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
36Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
38Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
384 data points

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