Citation:

PANGAEA.912775

Name: Seawater carbonate chemistry and metabolism of bacterial communities from coastal waters
Published: 2018
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Biomass/Abundance/Elemental composition; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Entire community; Laboratory experiment; North Atlantic; Other; Other metabolic rates; Pelagos; Respiration; Temperate

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Abstract:

Microcosm experiments to assess the bacterioplankton's response to phytoplankton-derived organic matter obtained under current and future ocean CO2 levels were performed. Surface seawater enriched with inorganic nutrients was bubbled for 8 days with air (current CO2 scenario) or with a 1000ppm CO2 air mixture (future CO2 scenario) under solar radiation. The organic matter produced under the current and future CO2 scenarios was subsequently used as an inoculum. Triplicate 12L flasks filled with 1.2µm of filtered natural seawater enriched with the organic matter inocula were incubated in the dark for 8 days under CO2 conditions simulating current and future CO2 scenarios, to study the bacterial response. The acidification of the media increased bacterial respiration at the beginning of the experiment, while the addition of the organic matter produced under future levels of CO2 was related to changes in bacterial production and abundance. This resulted in a 67% increase in the integrated bacterial respiration under future CO2 conditions compared to present CO2 conditions and 41% higher integrated bacterial abundance with the addition of the acidified organic matter compared to samples with the addition of non acidified organic matter. This study demonstrates that the increase in atmospheric CO2 levels can impact bacterioplankton metabolism directly, by changes in the respiration rate, and indirectly, by changes on the organic matter, which affected bacterial production and abundance.

Keyword(s):

Biomass/Abundance/Elemental composition; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Entire community; Laboratory experiment; North Atlantic; Other; Other metabolic rates; Pelagos; Respiration; Temperate

Supplement to:

Fuentes-Lema, Antonio; Sanleón-Bartolomé, Henar; Lubian, L M; Sobrino, Cristina (2018): Effects of elevated CO2 and phytoplankton-derived organic matter on the metabolism of bacterial communities from coastal waters. Biogeosciences, 15(22), 6927-6940, https://doi.org/10.5194/bg-15-6927-2018

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 42.230000 * Longitude: -8.780000

Date/Time Start: 2013-06-27T00:00:00 * Date/Time End: 2013-06-27T00:00:00

Event(s):

Ria_de_Vigo * Latitude: 42.230000 * Longitude: -8.780000 * Date/Time: 2013-06-27T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-02-28.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Sobrino, Cristina		Study
2	Treatment	Treat		Sobrino, Cristina
3	Experiment duration	Exp duration	days	Sobrino, Cristina
4	Chlorophyll a	Chl a	µg/l	Sobrino, Cristina
5	Chlorophyll a, standard deviation	Chl a std dev	±	Sobrino, Cristina
6	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Sobrino, Cristina
7	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Sobrino, Cristina
8	Carbon, organic, dissolved	DOC	µmol/l	Sobrino, Cristina
9	Carbon, organic, dissolved, standard deviation	DOC std dev	±	Sobrino, Cristina
10	pH	pH		Sobrino, Cristina		total scale
11	pH, standard deviation	pH std dev	±	Sobrino, Cristina		total scale
12	Respiration rate, oxygen	Resp O2	µmol/l/day	Sobrino, Cristina
13	Respiration rate, oxygen, standard deviation	Resp O2 std dev	±	Sobrino, Cristina
14	Respiration rate, oxygen, cumulative	Resp O2 cum	µmol/l	Sobrino, Cristina
15	Respiration rate, oxygen, cumulative, standard deviation	Resp O2 cum std dev	±	Sobrino, Cristina
16	Bacteria	Bact	#/ml	Sobrino, Cristina		active
17	Bacteria, standard deviation	Bact std dev	±	Sobrino, Cristina		active
18	Bacteria	Bact	#/ml	Sobrino, Cristina
19	Bacteria, standard deviation	Bact std dev	±	Sobrino, Cristina
20	Bacteria	Bact	#/ml	Sobrino, Cristina		cumulative
21	Bacteria, standard deviation	Bact std dev	±	Sobrino, Cristina		cumulative
22	Bacterial production	BP	µg/l/day	Sobrino, Cristina
23	Bacterial production, standard deviation	BP std dev	±	Sobrino, Cristina
24	Bacterial production	BP	µg/l	Sobrino, Cristina		cumulative
25	Bacterial production, standard deviation	BP std dev	±	Sobrino, Cristina		cumulative
26	Bacteria, carbon demand	BCD	µmol/l/day	Sobrino, Cristina
27	Bacteria, carbon demand, standard deviation	BCD std dev	±	Sobrino, Cristina
28	Bacteria, carbon demand, cumulative	BCD cum	µmol/l	Sobrino, Cristina		cumulative
29	Bacteria, carbon demand, cumulative, standard deviation	BCD cum std dev	±	Sobrino, Cristina		cumulative
30	Bacteria, growth efficiency	Bact growth eff		Sobrino, Cristina
31	Bacteria, growth efficiency, standard deviation	Bact growth eff std dev	±	Sobrino, Cristina
32	Bacteria, growth efficiency	Bact growth eff		Sobrino, Cristina		cumulative
33	Bacteria, growth efficiency, standard deviation	Bact growth eff std dev	±	Sobrino, Cristina		cumulative
34	pH	pH		Sobrino, Cristina		total scale
35	pH, standard deviation	pH std dev	±	Sobrino, Cristina		total scale
36	Temperature, water	Temp	°C	Sobrino, Cristina
37	Temperature, water, standard deviation	Temp std dev	±	Sobrino, Cristina
38	Salinity	Sal		Sobrino, Cristina
39	Salinity, standard deviation	Sal std dev	±	Sobrino, Cristina
40	Carbon dioxide	CO2	µmol/kg	Sobrino, Cristina
41	Carbon dioxide, standard deviation	CO2 std dev	±	Sobrino, Cristina
42	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Sobrino, Cristina
43	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Sobrino, Cristina
44	Bicarbonate ion	[HCO3]-	µmol/kg	Sobrino, Cristina
45	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Sobrino, Cristina
46	Carbonate ion	[CO3]2-	µmol/kg	Sobrino, Cristina
47	Carbonate ion, standard deviation	[CO3]2- std dev	±	Sobrino, Cristina
48	Carbon, inorganic, dissolved	DIC	µmol/kg	Sobrino, Cristina
49	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Sobrino, Cristina
50	Alkalinity, total	AT	µmol/kg	Sobrino, Cristina
51	Alkalinity, total, standard deviation	AT std dev	±	Sobrino, Cristina
52	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
54	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
55	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
57	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
59	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
61	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
63	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	Alkalinity, total, standard deviation	AT std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
65	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
66	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
67	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
68	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2520 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (Study)	2 Treat	3 Exp duration [days]	4 Chl a [µg/l]	5 Chl a std dev [±]	6 pCO2water_SST_wet [µatm]	7 pCO2 std dev [±]	8 DOC [µmol/l]	9 DOC std dev [±]	10 pH (total scale)	11 pH std dev [±] (total scale)	12 Resp O2 [µmol/l/day]	13 Resp O2 std dev [±]	14 Resp O2 cum [µmol/l]	15 Resp O2 cum std dev [±]	16 Bact [#/ml] (active)	17 Bact std dev [±] (active)	18 Bact [#/ml]	19 Bact std dev [±]	20 Bact [#/ml] (cumulative)	21 Bact std dev [±] (cumulative)	22 BP [µg/l/day]	23 BP std dev [±]	24 BP [µg/l] (cumulative)	25 BP std dev [±] (cumulative)	26 BCD [µmol/l/day]	27 BCD std dev [±]	28 BCD cum [µmol/l] (cumulative)	29 BCD cum std dev [±] (cumulative)	30 Bact growth eff	31 Bact growth eff std dev [±]	32 Bact growth eff (cumulative)	33 Bact growth eff std dev [±] (cumulative)	34 pH (total scale)	35 pH std dev [±] (total scale)	36 Temp [°C]	37 Temp std dev [±]	38 Sal	39 Sal std dev [±]	40 CO2 [µmol/kg]	41 CO2 std dev [±]	42 pCO2water_SST_wet [µatm]	43 pCO2 std dev [±]	44 [HCO3]- [µmol/kg]	45 [HCO3]- std dev [±]	46 [CO3]2- [µmol/kg]	47 [CO3]2- std dev [±]	48 DIC [µmol/kg]	49 DIC std dev [±]	50 AT [µmol/kg]	51 AT std dev [±]	52 CSC flag (Calculated using seacarb afte...)	53 CO2 [µmol/kg] (Calculated using seacarb afte...)	54 CO2 std dev [±] (Calculated using seacarb afte...)	55 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	56 fCO2 std dev [±] (Calculated using seacarb afte...)	57 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	58 pCO2 std dev [±] (Calculated using seacarb afte...)	59 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	60 [HCO3]- std dev [±] (Calculated using seacarb afte...)	61 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	62 [CO3]2- std dev [±] (Calculated using seacarb afte...)	63 AT [µmol/kg] (Calculated using seacarb afte...)	64 AT std dev [±] (Calculated using seacarb afte...)	65 Omega Arg (Calculated using seacarb afte...)	66 Omega Arg std dev [±] (Calculated using seacarb afte...)	67 Omega Cal (Calculated using seacarb afte...)	68 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	High carbon	0	14.46	3.28	418.66	20.50																											8.03	0.00	22.37	0.51	33.14	0.00	12.85	0.65	418.65	20.50	1299.04	64.02	93.66	4.64	1405.55	69.15	1569.11	72.91	9	8.92	0.48	290.50	15.23	291.46	15.28	1268.87	62.59	127.76	7.78	1607.05	75.21	2.03	0.16	3.11	0.24
laboratory	High carbon	1	21.33	4.11	735.82	396.37																											7.88	0.16	22.00	0.72	33.52	0.01	22.93	12.79	735.82	396.37	1503.73	268.18	77.47	15.85	1604.13	265.35	1723.35	217.06	9	14.79	6.27	478.17	202.40	479.76	203.07	1484.05	247.45	105.29	40.76	1756.84	287.17	1.66	0.65	2.55	1.00
laboratory	High carbon	2	8.85	3.77	913.14	510.85																											7.94	0.18	20.50	0.35	33.89	0.01	29.47	16.62	913.14	510.85	2100.05	638.51	114.91	10.95	2244.43	645.14	2400.76	591.69	9	18.44	9.71	573.50	301.92	575.44	302.95	2065.50	596.20	160.48	77.72	2454.74	692.46	2.52	1.22	3.87	1.88
laboratory	High carbon	3	3.24	0.29	911.51	454.82																											7.84	0.16	19.07	0.67	34.26	0.01	30.42	15.19	911.51	454.82	1750.73	443.87	74.45	6.14	1855.61	453.10	1955.95	413.47	9	20.03	9.14	599.67	273.48	601.74	274.42	1733.32	424.20	102.26	44.01	1992.50	477.17	1.59	0.69	2.46	1.06
laboratory	High carbon	4	5.23	0.15	1194.56	178.46																											7.73	0.04	19.63	0.35	34.64	0.02	39.15	5.51	1194.56	178.46	1943.31	121.61	67.52	3.11	2049.98	126.27	2123.76	117.76	9	28.30	3.25	862.60	98.81	865.55	99.15	1930.58	119.08	91.10	10.39	2157.75	131.00	1.42	0.17	2.18	0.27
laboratory	High carbon	5	3.74	2.23	1226.76	148.50																											7.74	0.04	18.70	0.44	35.01	0.02	41.27	5.43	1226.76	148.50	2044.23	91.35	70.27	4.32	2155.77	93.32	2230.03	81.27	9	29.59	3.14	880.51	93.19	883.56	93.52	2030.69	88.16	95.49	10.10	2267.07	97.44	1.48	0.17	2.28	0.26
laboratory	High carbon	6	3.01	0.36	1197.01	95.21																											7.75	0.02	19.07	0.21	35.14	0.16	39.78	3.04	1197.01	95.21	2038.25	106.18	72.51	2.68	2150.54	111.37	2230.08	110.00	9	28.55	2.07	858.96	62.29	861.93	62.51	2022.99	104.86	99.00	7.55	2268.06	115.34	1.53	0.14	2.36	0.21
laboratory	High carbon	7	3.93	0.91	1058.18	56.71																											7.76	0.01	19.23	0.55	34.88	0.20	35.07	2.15	1058.18	56.71	1867.55	66.22	69.08	2.41	1971.70	69.18	2053.88	67.48	9	25.52	1.21	770.33	35.47	772.98	35.59	1853.36	65.13	92.82	5.25	2086.77	71.77	1.44	0.11	2.22	0.16
laboratory	Low carbon	0	14.46	3.28	418.66	20.50																											8.03	0.00	22.37	0.51	33.14	0.00	12.85	0.65	418.65	20.50	1299.04	64.02	93.66	4.64	1405.55	69.15	1569.11	72.91	9	8.92	0.48	290.50	15.23	291.46	15.28	1268.87	62.59	127.76	7.78	1607.05	75.21	2.03	0.16	3.11	0.24
laboratory	Low carbon	1	22.12	9.15	264.05	17.17																											8.10	0.02	22.87	0.15	33.48	0.02	7.98	0.54	264.05	17.16	963.23	18.00	83.86	3.91	1055.07	17.19	1228.23	15.96	9	5.52	0.31	182.70	10.29	183.30	10.33	935.85	16.26	113.70	6.17	1259.78	21.22	1.80	0.13	2.76	0.20
laboratory	Low carbon	2	9.29	0.14	235.04	79.23																											8.22	0.06	19.53	0.31	33.82	0.05	7.75	2.56	235.04	79.23	1139.01	261.02	117.06	16.34	1263.82	278.04	1484.32	280.57	9	5.27	1.42	159.56	43.02	160.11	43.16	1101.55	243.08	156.99	39.78	1526.97	313.06	2.45	0.63	3.79	0.98
laboratory	Low carbon	3	3.75	0.76	226.17	38.11																											8.14	0.02	19.97	0.45	34.17	0.07	7.37	1.21	226.17	38.11	926.95	105.97	81.48	6.50	1015.80	113.00	1188.57	115.00	9	5.12	0.64	157.21	19.44	157.74	19.50	901.17	100.41	109.50	13.53	1219.30	124.99	1.71	0.23	2.64	0.35
laboratory	Low carbon	4	4.79	0.42	305.09	63.79																											8.08	0.04	20.00	0.53	34.50	0.09	9.91	2.09	305.09	63.79	1101.90	133.11	86.11	4.98	1197.92	138.49	1364.93	134.51	9	7.00	1.08	215.35	33.20	216.08	33.32	1076.03	124.79	114.89	16.92	1395.85	152.01	1.79	0.28	2.76	0.43
laboratory	Low carbon	5	3.07	0.48	371.53	27.10																											8.06	0.02	19.03	0.15	34.85	0.11	12.38	0.85	371.53	27.10	1310.44	34.00	96.15	2.49	1418.96	33.95	1589.15	30.35	9	8.90	0.52	266.93	15.53	267.85	15.59	1282.92	31.35	127.15	7.38	1622.99	38.16	1.97	0.15	3.04	0.23
laboratory	Low carbon	6	3.96	0.41	368.50	38.01																											8.08	0.01	18.77	0.06	35.23	0.28	12.34	1.25	368.50	38.01	1358.45	121.23	103.42	8.51	1474.21	130.81	1655.09	137.89	9	8.81	0.83	263.13	24.72	264.04	24.81	1327.75	117.92	137.64	13.27	1692.75	142.41	2.13	0.23	3.28	0.35
laboratory	Low carbon	7	4.02	1.54	279.98	36.16																											8.12	0.00	18.80	0.66	34.46	0.24	9.40	1.05	279.98	36.16	1127.98	134.61	93.19	12.58	1230.56	148.23	1408.31	160.67	9	6.70	0.82	199.40	24.29	200.09	24.38	1100.79	132.68	123.06	15.56	1440.80	162.37	1.91	0.26	2.95	0.40
laboratory	High carbon	0					79.14	0.00																									8.03	0.00	22.37	0.51	33.14	0.00	12.85	0.65	418.65	20.50	1299.04	64.02	93.66	4.64	1405.55	69.15	1569.11	72.91	9	8.92	0.48	290.50	15.23	291.46	15.28	1268.87	62.59	127.76	7.78	1607.05	75.21	2.03	0.16	3.11	0.24
laboratory	High carbon	5					122.94	12.10																									7.74	0.04	18.70	0.44	35.01	0.02	41.27	5.43	1226.76	148.50	2044.23	91.35	70.27	4.32	2155.77	93.32	2230.03	81.27	9	29.59	3.14	880.51	93.19	883.56	93.52	2030.69	88.16	95.49	10.10	2267.07	97.44	1.48	0.17	2.28	0.26
laboratory	High carbon	7					137.48	6.83																									7.76	0.01	19.23	0.55	34.88	0.20	35.07	2.15	1058.18	56.71	1867.55	66.22	69.08	2.41	1971.70	69.18	2053.88	67.48	9	25.52	1.21	770.33	35.47	772.98	35.59	1853.36	65.13	92.82	5.25	2086.77	71.77	1.44	0.11	2.22	0.16
laboratory	Low carbon	0					79.14	0.00																									8.03	0.00	22.37	0.51	33.14	0.00	12.85	0.65	418.65	20.50	1299.04	64.02	93.66	4.64	1405.55	69.15	1569.11	72.91	9	8.92	0.48	290.50	15.23	291.46	15.28	1268.87	62.59	127.76	7.78	1607.05	75.21	2.03	0.16	3.11	0.24
laboratory	Low carbon	5					129.28	13.43																									8.06	0.02	19.03	0.15	34.85	0.11	12.38	0.85	371.53	27.10	1310.44	34.00	96.15	2.49	1418.96	33.95	1589.15	30.35	9	8.90	0.52	266.93	15.53	267.85	15.59	1282.92	31.35	127.15	7.38	1622.99	38.16	1.97	0.15	3.04	0.23
laboratory	Low carbon	7					154.17	11.44																									8.12	0.00	18.80	0.66	34.46	0.24	9.40	1.05	279.98	36.16	1127.98	134.61	93.19	12.58	1230.56	148.23	1408.31	160.67	9	6.70	0.82	199.40	24.29	200.09	24.38	1100.79	132.68	123.06	15.56	1440.80	162.37	1.91	0.26	2.95	0.40
laboratory	Low carbon, non-acidified organic matter	0			396.96	17.84	88.98	0.00	8.07	0.00	0.51	0.00	0.51	0.00			62496.55	3842.76	62496.55	3842.76	1.10	0.11	1.10	0.11	0.60	0.01	0.60	0.01	0.15	0.01	0.15	0.01	8.07	0.00	15.06	0.00	34.60	0.00	14.88	0.67	396.96	17.84	1501.26	67.44	99.35	4.46	1615.48	72.57	1778.26	76.37	9	10.96	0.53	292.59	14.16	293.65	14.21	1476.51	66.45	128.01	7.05	1810.75	77.96	1.96	0.14	3.06	0.22
laboratory	Low carbon, non-acidified organic matter	1			421.13	8.97	92.66	6.55	8.08	0.02							77701.15	10064.63	140197.70	10773.28	87.45	14.29	88.54	14.29	11.73	1.23	12.33	1.23	0.65	0.05	0.80	0.05	8.08	0.02	15.27	0.31	34.50	0.00	15.69	0.19	421.13	8.97	1612.19	30.23	108.89	4.81	1736.77	34.84	1909.39	41.42	9	11.45	0.65	307.27	17.32	308.38	17.38	1583.99	32.58	141.33	8.17	1946.50	39.04	2.17	0.16	3.38	0.25
laboratory	Low carbon, non-acidified organic matter	2			568.67	186.50			8.06	0.09	4.12	0.73	4.63	0.73							207.59	5.78	296.13	15.42									8.05	0.10	15.06	0.00	34.30	0.00	21.35	7.00	568.67	186.50	1965.11	270.73	122.15	11.18	2108.60	267.60	2284.15	236.48	9	15.08	4.18	401.74	111.43	403.20	111.84	1934.47	247.43	159.05	39.97	2327.01	290.63	2.44	0.62	3.81	0.97
laboratory	Low carbon, non-acidified organic matter	3			425.91	66.48	111.58	6.22	8.06	0.06							545368.89	55690.17	685566.59	56722.65	196.52	11.59	492.65	19.29	20.96	2.52	33.29	2.80	0.78	0.09	1.58	0.11	8.06	0.06	15.06	0.00	34.50	0.00	15.97	2.49	425.91	66.48	1549.36	44.21	99.56	9.77	1664.88	37.05	1824.75	17.36	9	11.59	1.75	309.21	46.57	310.33	46.74	1524.43	37.25	128.86	17.29	1858.61	48.20	1.98	0.28	3.08	0.44
laboratory	Low carbon, non-acidified organic matter	4			519.09	34.05			8.03	0.01	4.59	2.45	9.22	2.56							138.64	16.52	631.29	25.40									8.03	0.01	15.06	0.00	34.50	0.00	19.46	1.28	519.09	34.05	1789.30	106.07	107.82	6.73	1916.58	113.50	2077.22	118.61	9	14.37	0.95	383.21	25.50	384.59	25.60	1763.13	104.54	139.08	9.83	2113.49	121.08	2.14	0.18	3.33	0.28
laboratory	Low carbon, non-acidified organic matter	5			349.85	27.72			8.13	0.01							333564.44	33484.00	1019131.03	65868.33													8.13	0.01	15.06	0.00	34.50	0.00	13.12	1.04	349.85	27.72	1505.95	98.19	113.34	6.78	1632.40	105.41	1819.37	110.14	9	9.56	0.68	254.86	18.23	255.78	18.30	1476.24	95.48	146.61	10.97	1856.65	114.48	2.25	0.20	3.51	0.31
laboratory	Low carbon, non-acidified organic matter	6							8.09	0.01
laboratory	Low carbon, non-acidified organic matter	7			386.22	36.16	102.42	0.02	8.06	0.02	3.90	0.23	13.11	2.57			218515.56	16322.40	1237646.59	67860.57	95.35	13.22	726.64	28.63	11.84	0.68	45.14	2.88	0.67	0.02	2.25	0.11	8.09	0.01	15.06	0.00	34.50	0.00	14.48	1.36	386.22	36.16	1564.50	146.47	110.75	10.37	1689.74	158.19	1868.55	167.20	9	10.93	1.08	291.40	28.70	292.46	28.81	1539.39	144.21	139.43	14.10	1899.17	170.36	2.14	0.24	3.34	0.37
laboratory	Low carbon, acidified organic matter	0			396.96	17.84	88.98	0.00	8.07	0.00	0.51	0.00	0.51	0.00			62496.55	3842.76	62496.55	3842.76	1.10	0.11	1.10	0.11	0.60	0.01	0.60	0.01	0.15	0.01	0.15	0.01	8.07	0.00	15.06	0.00	34.60	0.00	14.88	0.67	396.96	17.84	1501.26	67.44	99.35	4.46	1615.48	72.57	1778.26	76.37	9	10.96	0.53	292.59	14.16	293.65	14.21	1476.51	66.45	128.01	7.05	1810.75	77.96	1.96	0.14	3.06	0.22
laboratory	Low carbon, acidified organic matter	1			468.98	18.73	92.89	0.81	8.07	0.01							139990.80	33319.14	202487.35	33540.00	82.80	23.73	83.90	23.73	13.51	1.87	14.11	1.87	0.51	0.07	0.66	0.07	8.07	0.01	15.17	0.29	35.30	0.00	17.45	0.76	468.98	18.73	1774.84	37.86	119.17	1.24	1911.46	38.47	2093.82	36.23	9	12.85	0.48	345.32	12.65	346.57	12.69	1744.29	35.60	154.32	6.86	2133.50	42.04	2.36	0.15	3.67	0.24
laboratory	Low carbon, acidified organic matter	2			537.81	24.30			8.05	0.02	6.61	1.24	7.12	1.24							202.02	27.81	285.92	36.56									8.05	0.02	15.06	0.00	35.40	0.00	20.06	0.91	537.81	24.30	1947.69	79.59	124.88	9.51	2092.63	88.10	2275.17	97.95	9	14.81	0.99	396.85	26.69	398.29	26.79	1916.23	81.07	161.59	10.99	2317.04	95.04	2.47	0.20	3.84	0.32
laboratory	Low carbon, acidified organic matter	3			488.92	54.10	111.23	4.61	8.03	0.03							766420.00	73536.74	968907.35	80824.40	191.97	11.38	477.89	38.29	19.49	2.65	33.60	3.24	0.70	0.05	1.36	0.09	8.03	0.03	15.06	0.00	35.40	0.00	18.24	2.02	488.92	54.10	1672.60	86.27	101.45	3.53	1792.29	87.76	1949.86	82.29	9	13.32	1.20	357.04	32.23	358.33	32.34	1646.38	81.08	132.59	11.54	1986.27	94.74	2.03	0.20	3.15	0.31
laboratory	Low carbon, acidified organic matter	4			499.63	26.17			8.07	0.01	5.90	1.02	13.02	1.61							129.23	8.98	607.12	39.33									8.07	0.01	15.06	0.00	35.40	0.00	18.64	0.98	499.63	26.17	1880.26	94.66	125.11	6.48	2024.01	101.87	2210.68	107.22	9	13.63	0.80	365.35	21.51	366.67	21.59	1847.26	93.17	163.11	10.31	2254.13	109.67	2.49	0.19	3.88	0.30
laboratory	Low carbon, acidified organic matter	5			384.23	38.84			8.12	0.01							335235.56	51695.81	1304142.91	95942.90													8.12	0.01	15.06	0.00	35.30	0.00	14.34	1.45	384.23	38.84	1648.27	183.70	124.86	15.31	1787.47	200.41	1986.84	215.01	9	10.64	1.24	285.09	33.15	286.13	33.27	1616.99	181.39	159.84	18.92	2024.89	217.27	2.44	0.31	3.80	0.48
laboratory	Low carbon, acidified organic matter	6							8.11	0.01
laboratory	Low carbon, acidified organic matter	7			383.54	23.06	111.67	9.07	8.08	0.03	1.98	0.05	15.00	1.61			226675.56	26707.09	1530818.47	99590.71	44.93	1.56	652.05	39.36	5.73	0.09	39.33	3.24	0.65	0.01	2.01	0.09	8.11	0.01	15.06	0.00	35.40	0.00	14.31	0.86	383.54	23.06	1609.22	136.22	119.42	13.03	1742.95	150.09	1935.61	164.07	9	10.63	0.97	284.89	26.05	285.92	26.14	1579.40	136.12	152.92	14.39	1972.16	162.51	2.34	0.24	3.64	0.38
laboratory	High carbon, non-acidified organic matter	0			396.96	17.84	88.98	0.00	8.07	0.00	0.51	0.00	0.51	0.00			62496.55	3842.76	62496.55	3842.76	1.10	0.11	1.10	0.11	0.60	0.01	0.60	0.01	0.15	0.01	0.15	0.01	8.07	0.00	15.06	0.00	34.60	0.00	14.88	0.67	396.96	17.84	1501.26	67.44	99.35	4.46	1615.48	72.57	1778.26	76.37	9	10.96	0.53	292.59	14.16	293.65	14.21	1476.51	66.45	128.01	7.05	1810.75	77.96	1.96	0.14	3.06	0.22
laboratory	High carbon, non-acidified organic matter	1			963.07	32.68			7.85	0.01									143029.38	25177.84	83.52	9.47	84.62	9.47	20.64	1.78	21.24	1.78	0.34	0.03	0.49	0.03	7.85	0.01	14.97	0.12	34.60	0.00	36.19	1.10	963.07	32.68	2213.98	61.39	88.72	2.78	2338.90	64.98	2442.40	66.68	9	27.14	1.10	722.21	29.41	724.83	29.52	2197.37	61.19	114.39	5.56	2476.74	67.66	1.75	0.12	2.73	0.18
laboratory	High carbon, non-acidified organic matter	2			1456.57	66.90			7.69	0.02	13.68	1.72	14.19	1.72							184.85	37.19	269.47	38.38									7.69	0.02	15.06	0.00	34.40	0.00	54.64	2.51	1456.57	66.90	2293.04	12.70	63.07	2.15	2410.76	13.07	2455.31	7.26	9	40.82	2.05	1088.07	54.78	1092.01	54.98	2287.64	12.84	82.30	4.65	2488.31	15.41	1.26	0.09	1.97	0.14
laboratory	High carbon, non-acidified organic matter	3			1938.03	89.34	117.04	0.00	7.57	0.02							616577.78	90823.29	679074.33	90904.55	176.35	12.62	445.82	40.40	19.14	1.54	40.39	2.35	0.80	0.04	1.28	0.06	7.57	0.02	15.06	0.00	34.50	0.00	72.67	3.35	1938.03	89.34	2333.21	27.74	49.16	2.54	2455.04	28.83	2459.81	31.04	9	54.91	2.78	1464.41	74.40	1469.72	74.67	2336.23	27.53	63.90	3.71	2492.05	29.80	0.98	0.07	1.53	0.11
laboratory	High carbon, non-acidified organic matter	4			1229.60	56.20			7.78	0.01	3.91	1.03	18.10	2.01							142.37	5.51	588.19	40.78									7.77	0.01	15.06	0.00	34.50	0.00	46.10	2.11	1229.60	56.20	2347.64	104.98	78.34	3.73	2472.09	110.58	2547.50	112.22	9	34.64	1.85	923.96	49.53	927.31	49.71	2336.17	104.56	101.27	6.08	2581.55	113.69	1.55	0.12	2.42	0.18
laboratory	High carbon, non-acidified organic matter	5			1011.09	7.48			7.82	0.01							337377.78	30598.56	1016452.11	95916.15													7.82	0.01	15.06	0.00	34.50	0.00	37.91	0.28	1011.09	7.48	2166.29	43.06	81.12	2.69	2285.33	45.99	2376.41	48.98	9	28.45	1.01	758.63	27.20	761.38	27.30	2152.20	43.48	104.68	4.67	2409.07	47.84	1.61	0.10	2.50	0.16
laboratory	High carbon, non-acidified organic matter	6							7.79	0.01
laboratory	High carbon, non-acidified organic matter	7			1093.11	49.22	80.76	2.50	7.72	0.01	2.28	0.39	20.39	2.04			278942.22	47045.63	1295394.33	106832.58	97.04	26.10	685.23	48.42	10.37	1.31	50.76	2.69	0.78	0.04	2.06	0.07	7.79	0.01	15.06	0.00	34.60	0.00	40.96	1.84	1093.11	49.22	2185.81	82.61	76.49	2.62	2303.27	86.84	2383.98	87.33	9	30.76	1.47	820.76	39.34	823.73	39.48	2173.62	82.03	98.90	5.43	2416.23	89.56	1.52	0.11	2.36	0.17
laboratory	High carbon, acidified organic matter	0			396.96	17.84	88.98	0.00	8.07	0.00	0.51	0.00	0.51	0.00			62496.55	3842.76	62496.55	3842.76	1.10	0.11	1.10	0.11	0.60	0.01	0.60	0.01	0.15	0.01	0.15	0.01	8.07	0.00	15.06	0.00	34.60	0.00	14.88	0.67	396.96	17.84	1501.26	67.44	99.35	4.46	1615.48	72.57	1778.26	76.37	9	10.96	0.53	292.59	14.16	293.65	14.21	1476.51	66.45	128.01	7.05	1810.75	77.96	1.96	0.14	3.06	0.22
laboratory	High carbon, acidified organic matter	1			727.51	58.92	95.14	3.04	7.91	0.07							203241.38	10630.98	265737.93	11304.18	91.99	3.72	93.08	3.72	20.31	6.21	20.91	6.21	0.39	0.12	0.54	0.12	7.91	0.07	14.97	0.06	34.50	0.00	27.33	2.21	727.51	58.92	2084.73	34.68	104.63	11.94	2216.69	44.40	2355.79	63.73	9	22.29	3.80	592.98	101.20	595.13	101.56	2070.90	44.37	123.50	19.58	2374.77	55.85	1.90	0.31	2.95	0.49
laboratory	High carbon, acidified organic matter	2			1217.45	46.17			7.72	0.07	12.43	6.21	12.94	6.21							194.81	13.59	287.89	14.09									7.72	0.07	15.06	0.00	35.30	0.00	45.45	1.72	1217.45	46.17	2234.26	48.29	72.41	0.39	2352.11	50.39	2421.96	48.43	9	36.83	6.15	986.55	164.74	990.13	165.34	2227.62	48.44	87.66	14.25	2442.76	57.65	1.34	0.23	2.09	0.35
laboratory	High carbon, acidified organic matter	3			2212.97	229.17	113.05	4.78	7.51	0.04							897742.22	39881.55	1163480.15	41452.65	192.59	10.41	480.48	17.52	20.31	1.31	41.22	6.34	0.79	0.05	1.33	0.13	7.51	0.04	15.06	0.00	35.40	0.00	82.56	8.55	2212.97	229.17	2303.18	27.13	42.66	4.18	2428.40	30.61	2414.10	24.98	9	61.89	5.83	1659.02	156.57	1665.04	157.14	2310.32	29.20	56.19	5.57	2448.18	33.31	0.86	0.09	1.34	0.15
laboratory	High carbon, acidified organic matter	4			1149.94	69.53			7.80	0.03	4.26	1.21	17.20	6.32							133.51	10.19	613.99	20.26									7.80	0.03	15.06	0.00	35.50	0.00	42.88	2.59	1149.94	69.53	2358.35	36.62	85.80	5.22	2487.03	38.80	2577.76	41.34	9	32.17	2.41	862.87	64.79	866.00	65.03	2343.48	37.10	111.38	8.49	2613.62	42.18	1.70	0.15	2.65	0.24
laboratory	High carbon, acidified organic matter	5			1093.02	60.81			7.81	0.01							293751.11	9451.34	1457231.26	42516.47													7.81	0.01	15.06	0.00	35.50	0.00	40.70	2.18	1093.02	60.81	2291.87	60.32	85.21	0.04	2417.79	62.46	2511.06	59.09	9	30.54	1.19	819.14	32.23	822.11	32.34	2276.53	58.95	110.72	5.25	2546.34	64.79	1.69	0.11	2.63	0.17
laboratory	High carbon, acidified organic matter	6							7.80	0.00
laboratory	High carbon, acidified organic matter	7			1082.51	6.10	80.94	1.99	7.74	0.02	2.50	0.18	19.70	6.32			265253.33	2601.47	1722484.59	42595.99	53.62	14.26	667.62	24.78	6.97	0.71	48.19	6.38	0.64	0.04	1.97	0.13	7.80	0.00	15.06	0.00	35.60	0.00	40.34	0.23	1082.51	6.10	2206.34	12.44	79.70	0.45	2326.38	13.11	2413.35	13.33	9	30.07	0.54	806.88	15.01	809.81	15.06	2191.90	12.82	104.41	3.49	2448.29	14.04	1.59	0.09	2.48	0.14
laboratory	Low carbon, non-acidified organic matter														28372.41	6933.67
laboratory	Low carbon, acidified organic matter														15052.88	4069.38
laboratory	High carbon, non-acidified organic matter														9489.66	1639.00
laboratory	High carbon, acidified organic matter														9691.95	2134.44