Citation:

PANGAEA.912222

Name: Seawater carbonate chemistry and coral calcification
Published: 2020-02-19
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Benthic animals; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Cnidaria; Laboratory experiment; Laboratory strains; Light; Not applicable; Single species; Stylophora pistillata

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

Coral reefs are constructed by calcifiers that precipitate calcium carbonate to build their shells or skeletons through the process of calcification. Accurately assessing coral calcification rates is crucial to determine the health of these ecosystems and their response to major environmental changes such as ocean warming and acidification. Several approaches have been used to assess rates of coral calcification but there is a real need to compare these approaches in order to ascertain that high quality and intercomparable results can be produced. Here, we assessed four methods (total alkalinity anomaly, calcium anomaly, 45Ca incorporation and 13C incorporation) to determine coral calcification of the reef-building coral Stylophora pistillata. Given the importance of environmental conditions on this process, the study was performed under two pH (ambient and low level) and two light (light and dark) conditions. Under all conditions, calcification rates estimated using the alkalinity and calcium anomaly techniques as well as 45Ca incorporation were highly correlated. Such a strong correlation between the alkalinity anomaly and 45Ca incorporation techniques has not been observed in previous studies and most probably results from improvements described in the present paper. The only method which provided calcification rates significantly different from the other three techniques was 13C incorporation. Calcification rates based on this method were consistently higher than those measured using the other techniques. Although reasons for these discrepancies remain unclear, the use of this technique for assessing calcification rates in corals is not recommended without further investigations.

Keyword(s):

Animalia; Benthic animals; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Cnidaria; Laboratory experiment; Laboratory strains; Light; Not applicable; Single species; Stylophora pistillata

Supplement to:

Gómez Batista, Miguel; Metian, Marc; Oberhänsli, F; Pouil, Simon; Swarzenski, Peter W; Tambutté, Eric; Gattuso, Jean-Pierre; Hernández, Carlos M Alonso; Gazeau, Frédéric (2020): Intercomparison of four methods to estimate coral calcification under various environmental conditions. Biogeosciences, 17(4), 887-899, https://doi.org/10.5194/bg-17-887-2020

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2020-02-17.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Gómez Batista, Miguel		study
2	Species	Species		Gómez Batista, Miguel
3	Registration number of species	Reg spec no		Gómez Batista, Miguel
4	Uniform resource locator/link to reference	URL ref		Gómez Batista, Miguel		WoRMS Aphia ID
5	Experiment	Exp		Gómez Batista, Miguel
6	Type	Type		Gómez Batista, Miguel
7	Type	Type		Gómez Batista, Miguel		2
8	Treatment	Treat		Gómez Batista, Miguel
9	Identification	ID		Gómez Batista, Miguel		Beaker
10	Date/time start	Date/time start		Gómez Batista, Miguel		Incubation
11	Date/time end	Date/time end		Gómez Batista, Miguel		Incubation
12	Incubation duration	Inc dur	h	Gómez Batista, Miguel
13	Size	Size	mm	Gómez Batista, Miguel		Coral
14	Mass	Mass	g	Gómez Batista, Miguel		Coral.air
15	Mass	Mass	g	Gómez Batista, Miguel		Coral.buoyancy
16	Dry mass	Dry m	g	Gómez Batista, Miguel		Coral.Skeleton
17	Mass	Mass	g	Gómez Batista, Miguel		Initial.SW.weight
18	Mass	Mass	g	Gómez Batista, Miguel		Fil.SW.weight
19	Mass	Mass	g	Gómez Batista, Miguel		Beaker.weight.at.T0
20	Mass	Mass	g	Gómez Batista, Miguel		Beaker.weight.at.Tf
21	Salinity	Sal		Gómez Batista, Miguel		T0
22	Salinity	Sal		Gómez Batista, Miguel		Tf
23	pH	pH		Gómez Batista, Miguel	Potentiometric	total scale, T0
24	pH	pH		Gómez Batista, Miguel	Potentiometric	total scale, Tf
25	Alkalinity, total	AT	µmol/kg	Gómez Batista, Miguel	Potentiometric titration	T0
26	Alkalinity, total, standard deviation	AT std dev	±	Gómez Batista, Miguel	Potentiometric titration	T0
27	Alkalinity, total	AT	µmol/kg	Gómez Batista, Miguel	Potentiometric titration	Tf
28	Alkalinity, total, standard deviation	AT std dev	±	Gómez Batista, Miguel	Potentiometric titration	Tf
29	Calcium	Ca2+	mmol/kg	Gómez Batista, Miguel		T0
30	Calcium ion, standard deviation	Ca2+ std dev	±	Gómez Batista, Miguel		T0
31	Calcium	Ca2+	mmol/kg	Gómez Batista, Miguel		Tf
32	Calcium ion, standard deviation	Ca2+ std dev	±	Gómez Batista, Miguel		Tf
33	Calcium-45 activity	45Ca	Bq	Gómez Batista, Miguel		Normalized
34	Calcium-45 activity	45Ca	Bq	Gómez Batista, Miguel		Total.activity.FE.Aliquots
35	Calcium-45 activity	45Ca	Bq	Gómez Batista, Miguel		Total.activity.aliquots.
36	Calcium-45 activity, standard deviation	45Ca std dev	±	Gómez Batista, Miguel		Total.activity.aliquots.
37	δ13C	δ13C	‰ PDB	Gómez Batista, Miguel		coral.bulk
38	δ13C	δ13C	‰ PDB	Gómez Batista, Miguel		coral
39	δ13C, standard deviation	δ13C std dev	±	Gómez Batista, Miguel		coral
40	δ13C	δ13C	‰ PDB	Gómez Batista, Miguel		water_T0
41	δ13C	δ13C	‰ PDB	Gómez Batista, Miguel		water_Tf
42	Calcification rate of calcium carbonate	Calc rate CaCO3	µmol/g/h	Gómez Batista, Miguel		based on the alkalinity anomaly technique
43	Calcification rate, standard error	Calc rate std e	±	Gómez Batista, Miguel		based on the alkalinity anomaly technique
44	Calcification rate of calcium carbonate	Calc rate CaCO3	µmol/g/h	Gómez Batista, Miguel		based on the calcium anomaly technique
45	Calcification rate, standard error	Calc rate std e	±	Gómez Batista, Miguel		based on the calcium anomaly technique
46	Calcification rate of calcium carbonate	Calc rate CaCO3	µmol/g/h	Gómez Batista, Miguel		based on 45Ca incorporation technique
47	Calcification rate, standard error	Calc rate std e	±	Gómez Batista, Miguel		based on 45Ca incorporation technique
48	Calcification rate of calcium carbonate	Calc rate CaCO3	µmol/g/h	Gómez Batista, Miguel		based on 13C incorporation technique
49	Calcification rate, standard error	Calc rate std e	±	Gómez Batista, Miguel		based on 13C incorporation technique
50	Temperature, water	Temp	°C	Gómez Batista, Miguel
51	Temperature, water, standard deviation	Temp std dev	±	Gómez Batista, Miguel
52	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	T0
54	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	T0
55	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	T0
56	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	T0
57	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	T0
58	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	T0
59	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	T0
60	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	T0
61	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	T0
62	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	T0
63	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	T0
64	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	T0
65	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	T0
66	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	T0
67	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	T0
68	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	T0
69	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Tf
70	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	Tf
71	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Tf
72	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	Tf
73	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Tf
74	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	Tf
75	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Tf
76	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	Tf
77	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Tf
78	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	Tf
79	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Tf
80	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	Tf
81	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Tf
82	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	Tf
83	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	Tf
84	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	Tf

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2610 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp	6 Type	7 Type (2)	8 Treat	9 ID (Beaker)	10 Date/time start (Incubation)	11 Date/time end (Incubation)	12 Inc dur [h]	13 Size [mm] (Coral)	14 Mass [g] (Coral.air)	15 Mass [g] (Coral.buoyancy)	16 Dry m [g] (Coral.Skeleton)	17 Mass [g] (Initial.SW.weight)	18 Mass [g] (Fil.SW.weight)	19 Mass [g] (Beaker.weight.at.T0)	20 Mass [g] (Beaker.weight.at.Tf)	21 Sal (T0)	22 Sal (Tf)	23 pH (total scale, T0, Potentiometric)	24 pH (total scale, Tf, Potentiometric)	25 AT [µmol/kg] (T0, Potentiometric titration)	26 AT std dev [±] (T0, Potentiometric titration)	27 AT [µmol/kg] (Tf, Potentiometric titration)	28 AT std dev [±] (Tf, Potentiometric titration)	29 Ca2+ [mmol/kg] (T0)	30 Ca2+ std dev [±] (T0)	31 Ca2+ [mmol/kg] (Tf)	32 Ca2+ std dev [±] (Tf)	33 45Ca [Bq] (Normalized)	34 45Ca [Bq] (Total.activity.FE.Aliquots)	35 45Ca [Bq] (Total.activity.aliquots.)	36 45Ca std dev [±] (Total.activity.aliquots.)	37 δ13C [‰ PDB] (coral.bulk)	38 δ13C [‰ PDB] (coral)	39 δ13C std dev [±] (coral)	40 δ13C [‰ PDB] (water_T0)	41 δ13C [‰ PDB] (water_Tf)	42 Calc rate CaCO3 [µmol/g/h] (based on the alkalinity anoma...)	43 Calc rate std e [±] (based on the alkalinity anoma...)	44 Calc rate CaCO3 [µmol/g/h] (based on the calcium anomaly ...)	45 Calc rate std e [±] (based on the calcium anomaly ...)	46 Calc rate CaCO3 [µmol/g/h] (based on 45Ca incorporation t...)	47 Calc rate std e [±] (based on 45Ca incorporation t...)	48 Calc rate CaCO3 [µmol/g/h] (based on 13C incorporation te...)	49 Calc rate std e [±] (based on 13C incorporation te...)	50 Temp [°C]	51 Temp std dev [±]	52 CSC flag (Calculated using seacarb afte...)	53 CO2 [µmol/kg] (T0, Calculated using seacarb ...)	54 CO2 std dev [±] (T0, Calculated using seacarb ...)	55 fCO2water_SST_wet [µatm] (T0, Calculated using seacarb ...)	56 fCO2 std dev [±] (T0, Calculated using seacarb ...)	57 pCO2water_SST_wet [µatm] (T0, Calculated using seacarb ...)	58 pCO2 std dev [±] (T0, Calculated using seacarb ...)	59 [HCO3]- [µmol/kg] (T0, Calculated using seacarb ...)	60 [HCO3]- std dev [±] (T0, Calculated using seacarb ...)	61 [CO3]2- [µmol/kg] (T0, Calculated using seacarb ...)	62 [CO3]2- std dev [±] (T0, Calculated using seacarb ...)	63 DIC [µmol/kg] (T0, Calculated using seacarb ...)	64 DIC std dev [±] (T0, Calculated using seacarb ...)	65 Omega Arg (T0, Calculated using seacarb ...)	66 Omega Arg std dev [±] (T0, Calculated using seacarb ...)	67 Omega Cal (T0, Calculated using seacarb ...)	68 Omega Cal std dev [±] (T0, Calculated using seacarb ...)	69 CO2 [µmol/kg] (Tf, Calculated using seacarb ...)	70 CO2 std dev [±] (Tf, Calculated using seacarb ...)	71 fCO2water_SST_wet [µatm] (Tf, Calculated using seacarb ...)	72 fCO2 std dev [±] (Tf, Calculated using seacarb ...)	73 pCO2water_SST_wet [µatm] (Tf, Calculated using seacarb ...)	74 pCO2 std dev [±] (Tf, Calculated using seacarb ...)	75 [HCO3]- [µmol/kg] (Tf, Calculated using seacarb ...)	76 [HCO3]- std dev [±] (Tf, Calculated using seacarb ...)	77 [CO3]2- [µmol/kg] (Tf, Calculated using seacarb ...)	78 [CO3]2- std dev [±] (Tf, Calculated using seacarb ...)	79 DIC [µmol/kg] (Tf, Calculated using seacarb ...)	80 DIC std dev [±] (Tf, Calculated using seacarb ...)	81 Omega Arg (Tf, Calculated using seacarb ...)	82 Omega Arg std dev [±] (Tf, Calculated using seacarb ...)	83 Omega Cal (Tf, Calculated using seacarb ...)	84 Omega Cal std dev [±] (Tf, Calculated using seacarb ...)
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-45Ca	Exp	45Ca	Ambient pH / Light	1	2017-08-23T08:15	2017-08-23T16:15	8.00	30.92	2.8370	1.1017	1.9648	300.02	300.00	329.88	329.86	39.7	41.9	8.05	8.04	2556.00	0.50	2204.70	1.10	11.1796	0.0000	11.0000	0.0059	16.8	74.3	78.50	1.90						3.28	0.01	3.17	0.11	3.41	0.08			25	0.5	8	11.68	0.24	421.41	7.15	422.76	7.18	1923.05	14.57	258.44	6.81	2193.16	8.26	3.95	0.19	5.97	0.28	10.04	0.24	366.61	7.15	367.79	7.18	1634.05	14.59	224.22	6.81	1868.31	8.30	3.36	0.19	5.08	0.28
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-45Ca	Exp	45Ca	Ambient pH / Light	2	2017-08-23T08:15	2017-08-23T16:15	8.00	34.36	3.1659	1.2952	2.1551	299.86	299.66	329.53	329.33	39.7	42.0	8.05	8.04	2556.00	0.50	2179.40	0.50	11.1796	0.0000	10.9719	0.0000	17.5	96.7	86.50	2.90						3.21	0.01	3.03	0.09	3.29	0.11			25	0.5	8	11.68	0.24	421.41	7.06	422.76	7.09	1923.05	14.42	258.44	6.74	2193.16	8.19	3.95	0.18	5.97	0.28	9.91	0.24	361.97	7.06	363.13	7.09	1613.23	14.42	221.79	6.74	1844.93	8.19	3.32	0.18	5.02	0.28
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-45Ca	Exp	45Ca	Ambient pH / Light	3	2017-08-23T08:15	2017-08-23T16:15	8.00	32.90	3.3660	1.4235	2.3570	300.63	300.62	329.87	329.86	39.7	42.0	8.05	8.09	2556.00	0.50	2211.40	0.50	11.1796	0.0000	10.9647	0.0025	17.2	89.7	78.20	2.30						2.69	0.01	2.89	0.04	2.77	0.08			25	0.5	8	11.68	0.21	421.41	6.30	422.76	6.32	1923.05	15.49	258.44	7.24	2193.16	8.76	3.95	0.20	5.97	0.30	8.69	0.21	317.48	6.30	318.50	6.32	1587.60	15.49	244.90	7.24	1841.19	8.76	3.66	0.20	5.54	0.30
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-45Ca	Exp	45Ca	Ambient pH / Light	4	2017-08-23T08:15	2017-08-23T16:15	8.00	35.27	3.0768	1.1351	2.0192	299.86	299.85	329.22	329.21	39.7	41.8	8.05	8.01	2556.00	0.50	2184.00	0.60	11.1796	0.0000	10.9554	0.0062	17.4	161.5	85.20	0.80						3.38	0.01	3.54	0.12	3.48	0.03			25	0.5	8	11.68	0.25	421.41	7.66	422.76	7.68	1923.05	13.91	258.44	6.50	2193.16	7.89	3.95	0.18	5.97	0.26	10.85	0.25	396.03	7.66	397.30	7.68	1647.49	13.91	210.56	6.50	1868.91	7.90	3.16	0.18	4.77	0.26
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-45Ca	Exp	45Ca	Ambient pH / Light	5	2017-08-23T08:15	2017-08-23T16:15	8.00	33.50	4.6937	1.7888	3.1587	299.81	299.79	331.21	331.19	39.7	41.9	8.05	8.04	2556.00	0.50	2141.60	0.00	11.1796	0.0000	10.9204	0.0008	17.2	72.0	95.70	2.60						2.41	0.00	2.68	0.02	2.53	0.07			25	0.5	8	11.68	0.23	421.41	6.94	422.76	6.96	1923.05	14.16	258.44	6.61	2193.16	8.06	3.95	0.18	5.97	0.27	9.74	0.23	355.50	6.94	356.64	6.96	1584.54	14.16	217.42	6.60	1811.70	8.05	3.26	0.18	4.92	0.27
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-45Ca	Exp	45Ca	Ambient pH / Light	6	2017-08-23T08:15	2017-08-23T16:15	8.00	32.27	4.5757	1.7513	3.1434	300.16	300.14	331.38	331.36	39.7	41.7	8.05	8.06	2556.00	0.50	2140.80	1.00	11.1796	0.0000	10.9701	0.0000	17.3	81.1	100.60	3.50						2.43	0.01	2.10	0.01	2.65	0.09			25	0.5	8	11.68	0.22	421.41	6.59	422.76	6.61	1923.05	14.46	258.44	6.75	2193.16	8.22	3.95	0.19	5.97	0.28	9.20	0.22	335.58	6.59	336.66	6.61	1566.48	14.48	224.20	6.75	1799.89	8.26	3.36	0.19	5.09	0.28
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-45Ca	Exp	45Ca	Ambient pH / Dark	22	2017-08-31T21:45	2017-09-01T06:55	9.17	44.40	4.6700	1.7145	2.9962	300.04	300.02	331.24	331.22	40.4	41.0	8.05	7.60	2535.90	1.80	2274.80	1.10	11.0985	0.0028	11.0685	0.0021	15.3	75.6	56.44	1.24						1.59	0.01	1.72	0.02	1.54	0.03			25	0.5	8	11.49	0.73	416.17	22.76	417.50	22.83	1899.36	7.67	258.91	3.52	2169.75	4.80	3.94	0.09	5.95	0.13	34.44	0.73	1251.51	22.74	1255.52	22.82	2026.38	7.56	99.19	3.52	2160.01	4.59	1.50	0.09	2.26	0.13
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-45Ca	Exp	45Ca	Ambient pH / Dark	23	2017-08-31T21:45	2017-09-01T06:55	9.17	44.18	5.0800	1.8538	3.2430	300.42	300.41	332.17	332.16	40.4	41.0	8.05	7.67	2535.90	1.80	2290.70	0.20	11.0985	0.0028	11.0000	0.0044	15.3	58.3	50.10	0.74						1.39	0.01	1.32	0.04	1.26	0.02			25	0.5	8	11.49	0.62	416.17	19.23	417.50	19.29	1899.36	8.71	258.91	4.02	2169.75	5.29	3.94	0.10	5.95	0.15	28.97	0.62	1052.66	19.21	1056.03	19.27	2002.50	8.56	115.16	4.02	2146.64	5.00	1.74	0.10	2.63	0.15
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-45Ca	Exp	45Ca	Ambient pH / Dark	24	2017-08-31T21:45	2017-09-01T06:55	9.17	45.70	5.6700	1.9856	3.3640	300.02	299.93	332.09	332.00	40.4	41.1	8.05	7.69	2535.90	1.80	2264.20	0.60	11.0985	0.0028	11.0581	0.0007	14.9	45.2	57.17	1.75						1.46	0.01	1.64	0.01	1.43	0.04			25	0.5	8	11.49	0.58	416.17	18.06	417.50	18.12	1899.36	8.92	258.91	4.12	2169.75	5.40	3.94	0.10	5.95	0.16	27.15	0.58	987.19	18.04	990.36	18.10	1966.40	8.79	118.65	4.12	2112.21	5.15	1.79	0.10	2.71	0.16
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-45Ca	Exp	45Ca	Ambient pH / Dark	25	2017-08-31T21:45	2017-09-01T06:55	9.17	46.90	6.2200	2.6445	4.1620	300.14	300.18	333.36	333.40	40.4	40.9	8.05	7.60	2535.90	1.80	2238.00	2.40	11.0985	0.0028	11.0871	0.0053	15.0	46.5	66.24	0.69						1.29	0.01	1.10	0.04	1.33	0.01			25	0.5	8	11.49	0.71	416.17	22.39	417.50	22.46	1899.36	7.55	258.91	3.46	2169.75	4.74	3.94	0.09	5.95	0.13	33.90	0.71	1231.08	22.40	1235.03	22.48	1993.37	7.68	97.38	3.46	2124.64	4.98	1.47	0.09	2.23	0.13
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-45Ca	Exp	45Ca	Ambient pH / Dark	26	2017-08-31T21:45	2017-09-01T06:55	9.17	44.71	6.3500	2.5095	3.9020	300.15	300.11	332.56	332.52	40.4	40.9	8.05	7.60	2535.90	1.80	2222.20	0.10	11.0985	0.0028	11.0538	0.0000	15.2	37.1	68.37	3.11						1.44	0.01	1.45	0.03	1.44	0.07			25	0.5	8	11.49	0.71	416.17	22.23	417.50	22.30	1899.36	7.50	258.91	3.43	2169.75	4.71	3.94	0.09	5.95	0.13	33.65	0.71	1222.19	22.20	1226.11	22.27	1978.97	7.32	96.68	3.43	2109.30	4.38	1.46	0.09	2.21	0.13
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-45Ca	Exp	45Ca	Ambient pH / Dark	27	2017-08-31T21:45	2017-09-01T06:55	9.17	42.52	7.3500	2.9258	4.9590	300.38	300.26	334.65	334.53	40.4	41.0	8.05	7.63	2535.90	1.80	2336.70	1.40	11.0985	0.0028	11.0000	0.0024	14.9	17.0	52.36	2.49						0.75	0.01	0.75	0.02	0.89	0.04			25	0.5	8	11.49	0.69	416.17	21.70	417.50	21.76	1899.36	8.28	258.91	3.82	2169.75	5.09	3.94	0.10	5.95	0.14	32.78	0.69	1191.18	21.69	1195.00	21.76	2066.64	8.22	108.40	3.82	2207.82	4.97	1.64	0.10	2.48	0.14
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-low pH -LIGHT-Ca45	Exp	45Ca	Low pH / Light	300	2018-07-31T08:30	2018-07-31T13:30	5.00	25.74	2.5212		1.8468	311.34	310.68	340.71	340.05	37.9	38.7	7.21	7.84	2558.40	0.30	2504.30	0.10	11.2812	0.0055	11.2851	0.0065	29.6		20.20	0.70						1.00	0.04	0.05	0.23	0.85	0.03			25	0.5	8	103.93	0.45	3716.12	13.77	3728.06	13.81	2447.57	11.97	45.78	5.68	2597.28	6.72	0.71	0.15	1.08	0.23	20.70	0.45	743.10	13.77	745.48	13.81	2089.64	11.97	169.60	5.68	2279.93	6.71	2.62	0.15	3.96	0.23
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-low pH -LIGHT-Ca45	Exp	45Ca	Low pH / Light	301	2018-07-31T08:30	2018-07-31T13:30	5.00	27.86	2.8850		2.1170	314.54	314.09	344.08	343.63	37.9	38.7	7.21	7.85	2558.40	0.30	2519.40	0.10	11.2812	0.0055	11.2757	0.0000	28.5		15.30	0.40						0.66	0.03	0.33	0.07	0.58	0.02			25	0.5	8	103.93	0.45	3716.12	13.51	3728.06	13.55	2447.57	12.23	45.78	5.80	2597.28	6.85	0.71	0.15	1.08	0.23	20.27	0.45	727.89	13.51	730.22	13.55	2094.53	12.23	173.95	5.80	2288.75	6.85	2.68	0.15	4.06	0.23
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-low pH -LIGHT-Ca45	Exp	45Ca	Low pH / Light	302	2018-07-31T08:30	2018-07-31T13:30	5.00	27.40	3.0987		2.2938	307.63	307.49	338.63	338.49	37.9	38.6	7.21	7.85	2558.40	0.30	2492.30	1.70	11.2812	0.0055	11.2587	0.0055	28.0		22.50	0.30						0.96	0.04	0.75	0.16	0.80	0.01			25	0.5	8	103.93	0.44	3716.12	13.36	3728.06	13.41	2447.57	12.09	45.78	5.73	2597.28	6.78	0.71	0.15	1.08	0.23	20.07	0.44	720.17	13.37	722.48	13.42	2072.13	12.17	171.73	5.73	2263.94	6.96	2.65	0.15	4.01	0.23
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-low pH -LIGHT-Ca45	Exp	45Ca	Low pH / Light	303	2018-07-31T08:30	2018-07-31T13:30	5.00	25.01	2.7714		2.0909	310.40	310.38	340.27	340.25	37.9	38.6	7.21	7.80	2558.40	0.30	2493.40	1.00	11.2812	0.0055	11.0000	0.0073	27.2		23.40	0.40						1.04	0.04	1.06	0.23	0.94	0.02			25	0.5	8	103.93	0.50	3716.12	15.18	3728.06	15.23	2447.57	11.16	45.78	5.29	2597.28	6.30	0.71	0.14	1.08	0.21	22.95	0.50	823.56	15.18	826.20	15.23	2111.93	11.19	156.00	5.29	2290.88	6.36	2.41	0.14	3.64	0.21
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-low pH -LIGHT-Ca45	Exp	45Ca	Low pH / Light	304	2018-07-31T08:30	2018-07-31T13:30	5.00	24.12	2.9114		2.2202	296.31	295.71	326.49	325.89	37.9	38.7	7.21	7.84	2558.40	0.30	2507.20	1.20	11.2812	0.0055	11.2671	0.0068	28.1		20.00	0.90						0.75	0.03	0.52	0.19	0.73	0.03			25	0.5	8	103.93	0.46	3716.12	13.79	3728.06	13.83	2447.57	11.98	45.78	5.68	2597.28	6.73	0.71	0.15	1.08	0.23	20.72	0.46	743.99	13.79	746.37	13.84	2092.13	12.03	169.80	5.68	2282.65	6.81	2.62	0.15	3.96	0.23
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-low pH -DARK-Ca45	Exp	45Ca	Low pH / Dark	306	2018-08-02T20:00	2018-08-03T07:00	11.00	31.45	3.4286		2.6107	311.94	311.92	335.78	335.76	37.5	38.9	7.24	7.75	2552.90	2.40	1812.90	13.00	11.2776	0.0003	11.3264	0.0000	28.9		14.50	0.20						4.05	0.07	-0.01	0.00	0.20	0.00			25	0.5	8	96.78	0.41	3453.34	12.43	3464.44	12.47	2435.80	7.90	48.40	3.51	2580.98	5.05	0.75	0.09	1.14	0.14	18.80	0.43	675.71	13.38	677.88	13.42	1544.74	13.77	102.34	3.59	1665.87	13.17	1.58	0.09	2.38	0.14
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-low pH -DARK-Ca45	Exp	45Ca	Low pH / Dark	307	2018-08-02T20:00	2018-08-03T07:00	11.00	27.36	4.2681		3.3332	305.62	305.54	329.46	329.38	37.5	38.9	7.24	7.77	2552.90	2.40	2506.10	0.40	11.2776	0.0003	11.3246	0.0000	28.9		22.10	0.30						0.22	0.01	0.01	0.01	0.24	0.00			25	0.5	8	96.78	0.54	3453.34	16.47	3464.44	16.53	2435.80	10.92	48.40	5.08	2580.98	6.48	0.75	0.13	1.14	0.20	24.90	0.54	895.10	16.45	897.97	16.50	2142.72	10.72	148.64	5.08	2316.26	6.08	2.29	0.13	3.46	0.20
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-low pH -DARK-Ca45	Exp	45Ca	Low pH / Dark	308	2018-08-02T20:00	2018-08-03T07:00	11.00	27.59	3.4641		2.6937	293.86	293.67	317.70	317.51	37.5	38.9	7.24	7.78	2552.90	2.40	2508.00	0.40	11.2776	0.0003	11.3031	0.0028	28.1		22.10	0.10						0.25	0.01	0.22	0.03	0.30	0.00			25	0.5	8	96.78	0.53	3453.34	16.07	3464.44	16.12	2435.80	11.10	48.40	5.17	2580.98	6.57	0.75	0.14	1.14	0.20	24.27	0.53	872.45	16.05	875.24	16.10	2137.14	10.91	151.71	5.17	2313.12	6.18	2.34	0.14	3.53	0.20
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-low pH -DARK-Ca45	Exp	45Ca	Low pH / Dark	309	2018-08-02T20:00	2018-08-03T07:00	11.00	27.67	3.3653		2.3865	294.53	294.48	318.37	318.32	37.5	38.9	7.24	7.70	2552.90	2.40	2504.20	0.40	11.2776	0.0003	11.2953	0.0085	28.9		23.30	0.40						0.30	0.01	0.34	0.10	0.35	0.01			25	0.5	8	96.78	0.64	3453.34	19.64	3464.44	19.71	2435.80	9.73	48.40	4.50	2580.98	5.92	0.75	0.12	1.14	0.17	29.88	0.64	1074.09	19.62	1077.53	19.68	2188.43	9.50	129.21	4.50	2347.53	5.46	1.99	0.12	3.01	0.17
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-low pH -DARK-Ca45	Exp	45Ca	Low pH / Dark	310	2018-08-02T20:00	2018-08-03T07:00	11.00	30.25	3.5665		2.7609	294.44	293.92	318.28	317.76	37.5	38.8	7.24	7.77	2552.90	2.40	2459.10	0.10	11.2776	0.0003	11.2928	0.0041	28.6		16.10	0.10						0.48	0.01	0.32	0.04	0.21	0.00			25	0.5	8	96.78	0.53	3453.34	16.16	3464.44	16.21	2435.80	10.71	48.40	4.98	2580.98	6.38	0.75	0.13	1.14	0.20	24.45	0.53	878.23	16.14	881.05	16.19	2102.18	10.50	145.52	4.98	2272.15	5.96	2.24	0.13	3.39	0.19
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-13C	Exp	13C	Ambient pH / Light	8	2017-08-23T08:15	2017-08-23T16:15	8.00	36.65	3.3360	1.3257	2.3480	316.77	316.75	349.93	349.91	41.0	41.0	8.05	8.08	2619.98	0.71	2224.16	0.93	11.0000	0.0000	10.9285	0.0037					3.2	-1.37	2.00	1740	1655	3.26	0.01	3.29	0.06			1.92	1.35	25	0.5	8	11.81	0.22	429.19	6.52	430.57	6.54	1958.57	15.26	270.20	7.15	2240.58	8.62	4.08	0.20	6.17	0.30	9.11	0.22	330.92	6.52	331.98	6.55	1618.10	15.27	239.19	7.15	1866.41	8.63	3.61	0.20	5.46	0.30
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-13C	Exp	13C	Ambient pH / Light	9	2017-08-23T08:15	2017-08-23T16:15	8.00	33.31	3.4618	1.2584	2.5339	316.76	316.68	348.64	348.56	41.0	41.0	8.05	8.07	2619.98	0.71	2187.83	0.41	11.0000	0.0000	10.9167	0.0041					3.1	1.81	3.25	1740	1630	3.30	0.01	3.23	0.07			4.27	2.27	25	0.5	8	11.81	0.22	429.19	6.58	430.57	6.60	1958.57	14.85	270.20	6.94	2240.58	8.40	4.08	0.19	6.17	0.29	9.22	0.22	334.86	6.58	335.93	6.60	1600.11	14.84	231.15	6.94	1840.48	8.39	3.49	0.19	5.28	0.29
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-13C	Exp	13C	Ambient pH / Light	10	2017-08-23T08:15	2017-08-23T16:15	8.00	40.13	3.6662	1.4410	2.5958	316.60	316.54	349.41	349.35	41.0	41.0	8.05	8.11	2619.98	0.71	2205.08	1.44	11.0000	0.0000	10.9226	0.0019					4.3	3.44	5.13	1740	1638	3.09	0.01	3.06	0.03			5.47	3.66	25	0.5	8	11.81	0.20	429.19	5.98	430.57	6.00	1958.57	15.64	270.20	7.32	2240.58	8.84	4.08	0.21	6.17	0.31	8.26	0.20	300.10	5.98	301.06	6.00	1572.38	15.67	249.06	7.32	1829.70	8.91	3.76	0.21	5.69	0.31
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-13C	Exp	13C	Ambient pH / Light	11	2017-08-23T08:15	2017-08-23T16:15	8.00	36.09	4.4658	1.6709	3.1956	316.75	316.69	350.31	350.25	41.0	41.0	8.05	8.05	2619.98	0.71	2128.91	0.31	11.0000	0.0000	10.8703	0.0017					3.6	1.10	2.02	1740	1633	2.98	0.01	3.14	0.02			3.74	1.36	25	0.5	8	11.81	0.23	429.19	6.73	430.57	6.76	1958.57	14.12	270.20	6.59	2240.58	8.03	4.08	0.18	6.17	0.27	9.49	0.23	344.85	6.73	345.96	6.76	1573.70	14.11	217.10	6.59	1800.29	8.01	3.28	0.18	4.96	0.27
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-13C	Exp	13C	Ambient pH / Light	12	2017-08-23T08:15	2017-08-23T16:15	8.00	36.55	4.8301	2.0109	3.5287	316.76	316.75	350.87	350.86	41.0	41.0	8.05	8.04	2619.98	0.71	2112.06	0.46	11.0000	0.0000	11.0000	0.0056					2.9	0.76	0.73	1740	1631	2.80	0.01	2.92	0.06			3.49	0.41	25	0.5	8	11.81	0.23	429.19	6.85	430.57	6.88	1958.57	13.85	270.20	6.46	2240.58	7.89	4.08	0.18	6.17	0.27	9.69	0.23	352.04	6.85	353.16	6.88	1569.91	13.84	211.65	6.45	1791.25	7.87	3.20	0.18	4.83	0.27
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-LIGHT-13C	Exp	13C	Ambient pH / Light	13	2017-08-23T08:15	2017-08-23T16:15	8.00	35.35	5.7089	2.5090	4.4810	316.77	316.75	352.19	352.17	41.0	41.0	8.05	7.99	2619.98	0.71	1992.33	1.37	11.0000	0.0000	10.8058	0.0043					5.4	0.09	1.83	1740	1626	2.73	0.01	2.81	0.04			3.00	1.22	25	0.5	8	11.81	0.24	429.19	7.34	430.57	7.36	1958.57	12.29	270.20	5.70	2240.58	7.08	4.08	0.15	6.17	0.23	10.52	0.24	382.15	7.34	383.37	7.37	1518.87	12.33	182.50	5.70	1711.89	7.16	2.76	0.15	4.17	0.23
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-13C	Exp	13C	Ambient pH / Dark	15	2017-08-30T21:50	2017-08-31T07:00	9.17	52.28	5.3500	2.1794	3.9289	318.87	318.84	352.55	352.52	40.9	41.6	8.06	7.71	2615.20	0.60	2316.20	0.80	11.0965	0.0134	11.0000	0.0006					-0.2	-0.30	1.30	1634	1488	1.33	0.01	1.50	0.01			2.58	0.79	25	0.5	8	11.46	0.56	416.39	17.54	417.72	17.60	1944.47	9.37	273.96	4.40	2229.89	5.45	4.14	0.11	6.26	0.17	26.28	0.56	957.82	17.54	960.89	17.60	1997.27	9.38	127.44	4.40	2150.99	5.47	1.91	0.11	2.89	0.17
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-13C	Exp	13C	Ambient pH / Dark	16	2017-08-30T21:50	2017-08-31T07:00	9.17	50.37	6.4400	2.7587	4.7000	318.82	318.91	353.69	353.78	40.9	41.4	8.06	7.57	2615.20	0.60	2175.90	0.90	11.0965	0.0134	10.8822	0.0025					1.3	-3.00	0.50	1634	1459	1.63	0.01	1.63	0.02			0.68	0.23	25	0.5	8	11.46	0.74	416.39	23.41	417.72	23.48	1944.47	6.86	273.96	3.20	2229.89	4.19	4.14	0.08	6.26	0.12	35.42	0.74	1289.93	23.41	1294.07	23.48	1948.83	6.89	89.73	3.20	2073.99	4.24	1.35	0.08	2.04	0.12
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-13C	Exp	13C	Ambient pH / Dark	17	2017-08-30T21:50	2017-08-31T07:00	9.17	47.21	5.8200	2.2883	4.5660	318.88	318.88	353.47	353.47	40.9	41.3	8.06	7.73	2615.20	0.60	2393.20	1.60	11.0965	0.0134	10.9678	0.0008					0.5	-3.10	0.60	1634	1491	0.85	0.01	1.03	0.01			0.61	0.30	25	0.5	8	11.46	0.56	416.39	17.26	417.72	17.31	1944.47	9.96	273.96	4.69	2229.89	5.74	4.14	0.12	6.26	0.18	25.85	0.56	940.63	17.27	943.65	17.32	2054.24	10.04	136.45	4.69	2216.54	5.91	2.06	0.12	3.11	0.18
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-13C	Exp	13C	Ambient pH / Dark	18	2017-08-30T21:50	2017-08-31T07:00	9.17	51.30	6.6300	2.6208	4.8900	318.87	318.82	353.99	353.94	40.9	41.3	8.06	7.64	2615.20	0.60	2269.40	0.00	11.0965	0.0134	10.9176	0.0002					0.8	0.50	5.40	1634	1470	1.24	0.00	1.32	0.00			3.14	3.67	25	0.5	8	11.46	0.66	416.39	20.51	417.72	20.57	1944.47	8.10	273.96	3.79	2229.89	4.80	4.14	0.10	6.26	0.14	30.94	0.66	1125.92	20.50	1129.53	20.57	1998.66	8.08	107.91	3.79	2137.50	4.76	1.63	0.10	2.46	0.14
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-13C	Exp	13C	Ambient pH / Dark	19	2017-08-30T21:50	2017-08-31T07:00	9.17	49.70	6.8600	2.6285	5.0720	318.86	318.78	354.28	354.20	40.9	41.4	8.06	7.53	2615.20	0.60	2045.00	0.70	11.0965	0.0134	10.8012	0.0012					4.3	0.60	2.10	1634	1426	1.96	0.00	2.07	0.01			3.21	1.35	25	0.5	8	11.46	0.77	416.39	24.27	417.72	24.35	1944.47	6.01	273.96	2.79	2229.89	3.76	4.14	0.07	6.26	0.10	36.80	0.77	1339.90	24.27	1344.19	24.35	1846.21	6.02	77.53	2.79	1960.53	3.78	1.17	0.07	1.76	0.10
laboratory	Stylophora pistillata (coral)	206982	marinespecies.org	IC-DARK-13C	Exp	13C	Ambient pH / Dark	20	2017-08-30T21:50	2017-08-31T07:00	9.17	50.40	7.2300	2.7542	5.3330	318.81	318.83	353.86	353.88	40.9	41.6	8.06	7.50	2615.20	0.60	2182.20	0.60	11.0965	0.0134	10.8783	0.0037					1.6	0.70	6.10	1634	1462	1.42	0.00	1.46	0.02			3.28	4.16	25	0.5	8	11.46	0.88	416.39	27.94	417.72	28.02	1944.47	6.06	273.96	2.82	2229.89	3.82	4.14	0.07	6.26	0.10	42.35	0.88	1543.64	27.94	1548.59	28.02	1984.72	6.06	78.09	2.82	2105.16	3.82	1.17	0.07	1.77	0.10