Citation:

PANGAEA.911678

Name: Controlled lacustrine microcosms show a brGDGT response to environmental perturbations. Lipid data
Published: 2020-02-03
License: https://creativecommons.org/licenses/by/4.0/
Keywords: brGDGTs; lakes; microcosms; pH; Temperature

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Abstract:

In this work we used controlled microcosms to study the effect of temperature and pH on brGDGTs in lake water. We collected surface water from Kennedy Lake, Tucson, AZ, a shallow eutrophic artificial reservoir previously described by Martínez-Sosa & Tierney (2019). From the collected samples we set up a series of microcosms, consisting of 1L glass flasks filled with lake water, and manipulated single environmental factors including temperature and pH. For our temperature incubations, we selected four conditions (9C, 18C, 27C and 35C) and incubated 3 1L flasks under each condition for two periods of time (4 or 6 weeks). For the pH incubations, we used commercially available freshwater aquarium non-phosphate buffers (Proprietary composition, Seachem, Madison, GA, USA) to manipulate the pH of the microcosms. For these experiments we targeted four pH conditions (4, 5, 6 and 7), and included two control samples: one where we added enough buffer to maintain the initial pH (Control + Buffer), and another to which we added no buffer (Control - Buffer). GDGTs were analyzed on an Agilent 1260 Infinity HPLC coupled to an Agilent 6120 single quadrupole mass spectrometer using two BEH HILIC silica columns (2.1 x 150 mm, 1.7 um; Waters) and the methodology of Hopmans et al. (2016). We calculated peak areas using the MATLAB package software ORIGAmI (Fleming et al. 2016) and estimated the concentration of brGDGTs by comparing the obtained peaks with a C46 internal standard (Huguet et al. 2006) normalized to the volume of each sample.

Keyword(s):

brGDGTs; lakes; microcosms; pH; Temperature

Supplement to:

Martínez-Sosa, Pablo; Tierney, Jessica E; Meredith, Laura K (in press): Controlled lacustrine microcosms show a brGDGT response to environmental perturbations. Organic Geochemistry, 104041, https://doi.org/10.1016/j.orggeochem.2020.104041

Further details:

Fleming, Laura; Tierney, Jessica E (2016): An automated method for the determination of the TEX86 and paleotemperature indices. Organic Geochemistry, 92, 84-91, https://doi.org/10.1016/j.orggeochem.2015.12.011

Hopmans, Ellen C; Schouten, Stefan; Sinninghe Damsté, Jaap S (2016): The effect of improved chromatography on GDGT-based palaeoproxies. Organic Geochemistry, 93, 1-6, https://doi.org/10.1016/j.orggeochem.2015.12.006

Huguet, Carme; Hopmans, Ellen C; Febo-Ayala, Wilma; Thompson, David H; Sinninghe Damsté, Jaap S; Schouten, Stefan (2006): An improved method to determine the absolute abundance of glycerol dibiphytanyl glycerol tetraether lipids. Organic Geochemistry, 37(9), 1036-1041, https://doi.org/10.1016/j.orggeochem.2006.05.008

Martínez-Sosa, Pablo; Tierney, Jessica E (2019): Lacustrine brGDGT response to microcosm and mesocosm incubations. Organic Geochemistry, 127, 12-22, https://doi.org/10.1016/j.orggeochem.2018.10.011

Coverage:

Latitude: 32.229800 * Longitude: -110.954900

Event(s):

Kennedy_Lake_water_microcosm * Latitude: 32.229800 * Longitude: -110.954900 * Location: Tucson, Arizona, USA * Method/Device: Laboratory experiment

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Sample ID	Sample ID		Martínez-Sosa, Pablo
2	Experiment	Exp		Martínez-Sosa, Pablo
3	Incubation duration	Inc dur	weeks	Martínez-Sosa, Pablo
4	Temperature, water	Temp	°C	Martínez-Sosa, Pablo		incubation temperature
5	pH	pH		Martínez-Sosa, Pablo		incubation pH
6	Branched glycerol dialkyl glycerol tetraether, IIIa	brGDGT IIIa	ng/l	Martínez-Sosa, Pablo	Calculated
7	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
8	Branched glycerol dialkyl glycerol tetraether, IIIa'	brGDGT IIIa'	ng/l	Martínez-Sosa, Pablo	Calculated
9	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
10	Branched glycerol dialkyl glycerol tetraether, IIa	brGDGT IIa	ng/l	Martínez-Sosa, Pablo	Calculated
11	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
12	Branched glycerol dialkyl glycerol tetraether, IIa'	brGDGT IIa'	ng/l	Martínez-Sosa, Pablo	Calculated
13	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
14	Branched glycerol dialkyl glycerol tetraether, IIb	brGDGT IIb	ng/l	Martínez-Sosa, Pablo	Calculated
15	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
16	Branched glycerol dialkyl glycerol tetraether, IIb'	brGDGT IIb'	ng/l	Martínez-Sosa, Pablo	Calculated
17	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
18	Branched glycerol dialkyl glycerol tetraether, Ia	brGDGT Ia	ng/l	Martínez-Sosa, Pablo	Calculated
19	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
20	Branched glycerol dialkyl glycerol tetraether, Ib	brGDGT Ib	ng/l	Martínez-Sosa, Pablo	Calculated
21	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
22	Branched glycerol dialkyl glycerol tetraether, Ic	brGDGT Ic	ng/l	Martínez-Sosa, Pablo	Calculated
23	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
24	Branched glycerol dialkyl glycerol tetraether	brGDGT	ng/l	Martínez-Sosa, Pablo	Calculated	Total branched GDGTs
25	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
26	Cyclization ratio of branched tetraethers	CBT		Martínez-Sosa, Pablo	Calculated
27	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
28	Methylation index of 5-methyl branched glycerol dialkyl glycerol tetraether	MBT'5ME		Martínez-Sosa, Pablo	Calculated
29	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
30	Isomer ratio	IR		Martínez-Sosa, Pablo	Calculated
31	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated
32	Degree of cyclisation	DC		Martínez-Sosa, Pablo	Calculated	fC
33	Standard deviation	Std dev	±	Martínez-Sosa, Pablo	Calculated

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

528 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Sample ID	2 Exp	3 Inc dur [weeks]	4 Temp [°C] (incubation temperature)	5 pH (incubation pH)	6 brGDGT IIIa [ng/l]	7 Std dev [±]	8 brGDGT IIIa' [ng/l]	9 Std dev [±]	10 brGDGT IIa [ng/l]	11 Std dev [±]	12 brGDGT IIa' [ng/l]	13 Std dev [±]	14 brGDGT IIb [ng/l]	15 Std dev [±]	16 brGDGT IIb' [ng/l]	17 Std dev [±]	18 brGDGT Ia [ng/l]	19 Std dev [±]	20 brGDGT Ib [ng/l]	21 Std dev [±]	22 brGDGT Ic [ng/l]	23 Std dev [±]	24 brGDGT [ng/l] (Total branched GDGTs)	25 Std dev [±]	26 CBT	27 Std dev [±]	28 MBT'5ME	29 Std dev [±]	30 IR	31 Std dev [±]	32 DC (fC)	33 Std dev [±]
Time 0	Temperarute	0	24	8.5	1.093490917	0.126423496	2.009114123	0.149301773	2.709300222	0.142761336	4.318875523	0.163701928	0.982921630	0.019032491	1.519715096	0.060767378	4.083215158	0.347280403	1.579002962	0.083372025	0.120377534	0.105543678	18.416013160	0.968620354	0.004843369	0.025892472	0.546806872	0.017953027	0.621254160	0.013738843	0.117334895	0.007141293
9C 4w	Temperarute	4	9	8.5	0.377598212	0.085263351	0.824920186	0.130334901	1.197571411	0.232891717	1.992678106	0.277277748	0.420599272	0.060085868	0.667436439	0.135898228	2.102567214	0.461168757	0.844282070	0.153125023	0.072545813	0.064358163	8.500198723	1.559302292	-0.012072875	0.024342174	0.600435872	0.014290089	0.636508184	0.008476365	0.121676820	0.004317391
18C 4w	Temperarute	4	18	8.5	0.508660116	0.018071988	2.611521670	1.226181895	1.338513259	0.157727913	4.955861835	2.003672730	0.531954488	0.035310123	1.049864986	0.286270851	2.707151572	0.463969722	0.841866158	0.067368381	0.074675275	0.003412314	14.620069360	4.225841941	0.264033591	0.123181054	0.602610631	0.017836236	0.773558130	0.060097217	0.089791443	0.012418372
27C 4w	Temperarute	4	27	8.5	0.267076996	0.115882343	4.555646448	3.921752478	0.940543692	0.474777387	13.040853760	11.693354690	0.364355788	0.141257871	2.834205998	2.686513207	5.535231472	4.484841518	1.023728476	0.768826336	0.038781819	0.047355967	28.600424450	24.192864950	0.448453185	0.072190555	0.779196050	0.063807182	0.909643029	0.035576660	0.074864671	0.000989940
35C 4w	Temperarute	4	35	8.5	0.408924070	0.085082115	5.428624519	0.974442272	1.162926574	0.238907086	42.895538600	16.400926970	0.343804044	0.031113115	28.671613790	1.500080237	57.923901460	29.422651200	35.089889790	7.598157892	0.596853931	0.371165024	172.522076800	55.434653440	0.140298498	0.103377236	0.978462161	0.006965101	0.975079973	0.006114946	0.196403089	0.033866503
9C 6w	Temperarute	6	9	8.5	0.229545399	0.063490575	0.437293602	0.101610761	0.704420968	0.158296336	1.190787955	0.246712747	0.264812427	0.030783718	0.437030180	0.090272469	1.414232428	0.267604855	0.555326817	0.108911534	0.042400034	0.010691378	5.275849811	1.056784009	-0.047351490	0.014860370	0.627296095	0.010971563	0.632690028	0.001940089	0.127595157	0.004663964
18C 6w	Temperarute	6	18	8.5	0.316016338	0.052613365	3.074958412	1.358344360	1.004645833	0.140561874	5.676897616	2.498622659	0.396862775	0.069689250	0.993605609	0.298050909	2.730506511	0.645725291	0.822922046	0.064645399	0.077599430	0.013682366	15.094014570	4.989173954	0.363556290	0.122203163	0.677244318	0.035949652	0.838186551	0.058007729	0.081109184	0.012572501
27C 6w	Temperarute	6	27	8.5	0.274231433	0.141251541	8.923513998	2.169032091	0.774202108	0.380021268	25.829360840	6.139416193	0.342330428	0.109569549	10.683188670	3.700369882	12.282554180	2.836545880	3.348938840	0.901049927	0.094720518	0.027694388	62.553041010	16.054860170	0.532561268	0.042288128	0.921158021	0.014377244	0.971096480	0.005220017	0.115238220	0.014020229
35C 6w	Temperarute	6	35	8.5	0.383128810	0.046761603	8.023254603	4.660700280	0.956899681	0.193294095	50.734183110	25.519485130	0.535207186	0.244746788	43.493624370	13.970606180	59.927688250	24.228020130	49.711103100	13.196732780	1.170988086	0.507212610	214.936077200	77.607250290	0.223271232	0.037251905	0.982559985	0.004234756	0.980156341	0.006792875	0.228996814	0.032481380
T0	pH	4	24	8.5	0.885687696	0.042397288	2.175037882	0.105368390	2.830717681	0.285915074	5.354046571	0.542688479	1.122796429	0.125251459	1.892626598	0.152431588	5.175352404	0.526973252	2.059297649	0.200425360	0.205803830	0.050968402	22.138512910	2.018603226	0.045581730	0.007581937	0.604031763	0.005708805	0.657751175	0.001813387	0.136916319	0.002237559
C-B	pH	4	25	7.5	0.408834695	0.112826028	1.459250944	0.632878879	1.239341991	0.443952979	2.871118089	0.560241518	0.507684927	0.144276507	1.058373442	0.547622474	2.929189016	1.046141057	0.675149893	0.387864475	0.029587124	0.006269404	11.437335080	3.675223249	0.087214225	0.070008617	0.621618169	0.057087635	0.710643757	0.043771488	0.112804593	0.028093321
C+B	pH	4	25	8.3	0.210318201	0.065274076	1.735363351	0.308734627	0.869641746	0.223017347	4.482938707	1.251167990	0.338848200	0.094846792	1.265425966	0.211564733	2.894575027	0.383713418	0.851104635	0.066309745	0.059616119	0.024519419	12.884797480	2.110149786	0.279220953	0.083042653	0.728002978	0.051726784	0.832806737	0.056898236	0.110280532	0.013659688
pH7	pH	4	25	7.7	0.246874952	0.001129977	1.210912870	0.108534145	0.921156579	0.115030147	2.669555407	0.468518639	0.320604890	0.046873590	0.912035670	0.220753285	2.313332148	0.441423277	0.786285722	0.184434303	0.088818621	0.047176669	9.616912637	1.653398271	0.156344845	0.006578120	0.678308950	0.023535149	0.762476044	0.011963408	0.123738254	0.008956010
pH6	pH	4	25	6.7	0.140775078	0.126943347	0.732604781	0.520526761	0.610643083	0.462006768	1.758458467	1.295204758	0.194633714	0.165443543	0.557276161	0.476234067	1.527900379	1.240479466	0.538889043	0.428843324	0.042958567	0.041599665	6.198683921	4.823952791	0.153112127	0.029348329	0.684854502	0.008954373	0.765496015	0.009327219	0.119294757	0.007533722
pH5	pH	4	25	5.5	0.175251707	0.099142030	0.618162775	0.357036931	0.643419372	0.389744047	1.555399346	0.882385425	0.221223639	0.125241280	0.480447859	0.242689578	1.418837379	0.769855585	0.482299743	0.265061292	0.054903919	0.020335267	5.743292212	3.162035440	0.092779926	0.038274519	0.656066991	0.017629390	0.717000470	0.023587766	0.128356855	0.012839721
pH4	pH	4	25	4.3	0.371937113	0.033847923	1.286195491	0.081338530	1.397659972	0.114884073	3.088352923	0.312973797	0.431084728	0.199152640	0.913270398	0.414184586	3.072056861	0.154157163	1.150422208	0.024964226	0.104178019	0.047523657	12.108223900	1.218371961	0.059142554	0.041781940	0.659847818	0.016160834	0.703303739	0.005342365	0.127626563	0.015747271