<?xml version="1.0" encoding="UTF-8"?><!--*** Generated from internal PANGAEA metadata schema by dif.xslt ***--><DIF xsi:schemaLocation="http://gcmd.gsfc.nasa.gov/Aboutus/xml/dif/ http://gcmd.gsfc.nasa.gov/Aboutus/xml/dif/dif_v9.4.xsd" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns="http://gcmd.gsfc.nasa.gov/Aboutus/xml/dif/">
<Entry_ID>PANGAEA_911441</Entry_ID>
<Entry_Title>Results of the cold-water sponge Halichondria panicea exposure experiment to Benzo-A-Pyrene</Entry_Title>
<Data_Set_Citation>
<Dataset_Creator>Vad, Johanne; Durán Suja, Laura</Dataset_Creator>
<Dataset_Title>Results of the cold-water sponge Halichondria panicea exposure experiment to Benzo-A-Pyrene</Dataset_Title>
<Dataset_Release_Date>2020-01-28</Dataset_Release_Date>
<Dataset_Publisher>PANGAEA</Dataset_Publisher>
<Data_Presentation_Form>Dataset</Data_Presentation_Form>
<Online_Resource>https://doi.pangaea.de/10.1594/PANGAEA.911441</Online_Resource>
</Data_Set_Citation>
<Personnel>
<Role>Investigator</Role>
<First_Name>Johanne</First_Name>
<Last_Name>Vad</Last_Name>
</Personnel>
<Discipline>
<Discipline_Name>Earth Science</Discipline_Name>
</Discipline>
<Parameters>
<Detailed_Variable>Sample ID</Detailed_Variable>
</Parameters>
<Parameters>
<Detailed_Variable>Treatment</Detailed_Variable>
</Parameters>
<Parameters>
<Detailed_Variable>Time in hours</Detailed_Variable>
</Parameters>
<Parameters>
<Detailed_Variable>Halichondria panicea, heat shock protein 70, log fold change</Detailed_Variable>
</Parameters>
<Parameters>
<Detailed_Variable>Halichondria panicea, heat shock protein 90, log fold change</Detailed_Variable>
</Parameters>
<Parameters>
<Detailed_Variable>Halichondria panicea, cyclophilin</Detailed_Variable>
</Parameters>
<Parameters>
<Detailed_Variable>Halichondria panicea, cytochrome b5</Detailed_Variable>
</Parameters>
<ISO_Topic_Category>geoscientificInformation</ISO_Topic_Category>
<Keyword>Benzo-A-Pyrene</Keyword>
<Keyword>Poly Aromatic Hydrocarbons</Keyword>
<Keyword>qPCR</Keyword>
<Keyword>sponge</Keyword>
<Sensor_Name>
<Long_Name>Calculated</Long_Name>
</Sensor_Name>
<Data_Set_Progress>Complete</Data_Set_Progress>
<Project>
<Short_Name>ATLAS</Short_Name>
<Long_Name>A Trans-Atlantic assessment and deep-water ecosystem-based spatial management plan for Europe</Long_Name>
</Project>
<Access_Constraints>unrestricted</Access_Constraints>
<Use_Constraints>CC-BY-4.0: Creative Commons Attribution 4.0 International</Use_Constraints>
<Data_Set_Language>English</Data_Set_Language>
<Data_Center>
<Data_Center_Name>
<Short_Name>PANGAEA</Short_Name>
<Long_Name>Data Publisher for Earth &amp; Environmental Science</Long_Name>
</Data_Center_Name>
<Data_Center_URL>https://www.pangaea.de/</Data_Center_URL>
<Personnel>
<Role>Data Center Contact</Role>
<First_Name>Michael</First_Name>
<Last_Name>Diepenbroek</Last_Name>
<Email>info@pangaea.de</Email>
<Contact_Address>
<Address>Leobener Str.</Address>
<City>Bremen</City>
<Province_or_State>Bremen</Province_or_State>
<Postal_Code>28359</Postal_Code>
<Country>Germany</Country>
</Contact_Address>
</Personnel>
</Data_Center>
<Distribution>
<Distribution_Media>online</Distribution_Media>
<Distribution_Size>429 data points</Distribution_Size>
<Distribution_Format>text/tab-separated-values</Distribution_Format>
</Distribution>
<Summary>In this study, inter-tidal cold-water sponge Halichondria panicea samples were exposed to Benzo-A-pyrene for 48h (accumulation phase) and then left to recover for 13 days in clean seawater (depuration phase). Tissue samples were collected at multiple time points during the accumulation phase to conduct qPCR targeting several genes of interest. Tissue samples were also collected during the accumulation and depuration phases to determine concentration of hydrocarbon in the sponge tissue.  The files provided here detail (1) the hydrocarbon data determined by GC/MS analysis and (2) the sponge log fold change data for four genes of interest derived by qPCR. ** For all details see the full metadata description at "https://doi.pangaea.de/10.1594/PANGAEA.911441"!</Summary>
<Related_URL>
<URL>http://www.eu-atlas.org/</URL>
<Description>ATLAS</Description>
</Related_URL>
<Metadata_Name>DIF</Metadata_Name>
<Metadata_Version>9.4</Metadata_Version>
<DIF_Creation_Date>2020-01-28</DIF_Creation_Date>
<Last_DIF_Revision_Date>2026-07-15</Last_DIF_Revision_Date>
</DIF>
