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Koutsouveli, Vasiliki; Cárdenas, Paco; Rapp, Hans Tore; Riesgo, Ana (2020): Transcriptomic and histological analysis (Light and Transmission Electron Microscopy) on five Geodia species to identify their reproductive status and the gene expression during gametogenesis [dataset]. Natural History Museum, PANGAEA, https://doi.org/10.1594/PANGAEA.911273

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Abstract:
The collected samples of Geodia species were used to identify if they were reproductive and understand which genes are expressed during gametogenesis. Specifically, specimens fixed in glutaraldehyde solution, were processed for histological analysis to observe gametes in the sponge tissue. Once, we identified male, female (while in gametogenesis) and non-reproductive specimens, we used the RNAlater fixed samples to extract RNA from those specimens. Then, we prepared cDNA libraries, sequenced them with illumina nextseq and we did differential gene expression analysis in order to compare the expressed genes in male vs female specimens and vs non-reproductive specimens. The goal was to understand the molecular machinery of gametogenesis in sponges from an evolutionary point of view.
Keyword(s):
gametogenesis; Geodia sp.; Porifera; Transcriptomics
Supplement to:
Koutsouveli, Vasiliki; Cárdenas, Paco; Santodomingo, Nadia; Marina, Anabel; Morato, Esperanza; Rapp, Hans Tore; Riesgo, Ana (2020): The Molecular Machinery of Gametogenesis in Geodia Demosponges (Porifera): Evolutionary Origins of a Conserved Toolkit across Animals. Molecular Biology and Evolution, https://doi.org/10.1093/molbev/msaa183
Project(s):
Deep-sea Sponge Grounds Ecosystems of the North Atlantic (SponGES)
Funding:
Horizon 2020 (H2020), grant/award no. 679849: Deep-sea Sponge Grounds Ecosystems of the North Atlantic
Coverage:
Median Latitude: 67.382866 * Median Longitude: 8.832116 * South-bound Latitude: 58.876230 * West-bound Longitude: 5.373950 * North-bound Latitude: 73.982650 * East-bound Longitude: 21.375000
Date/Time Start: 2016-04-05T00:00:00 * Date/Time End: 2017-07-29T00:00:00
Minimum Elevation: -2959.0 m * Maximum Elevation: -267.0 m
Event(s):
GS2016109A-06-ROV-01  * Latitude Start: 73.815650 * Longitude Start: 7.519170 * Latitude End: 73.982650 * Longitude End: 7.523700 * Date/Time Start: 2016-06-20T15:34:00 * Date/Time End: 2016-06-21T00:10:00 * Elevation Start: -717.0 m * Elevation End: -640.0 m * Location: Schultz Bank * Campaign: GS2016109A (GS16A-202) * Basis: G. O. Sars (2003) * Method/Device: Remote operated vehicle (ROV) * Comment: 4 transects on summit (approx 900 m long and 100-120 m between lines)
GS2017110-09-ROV-6  * Latitude Start: 64.074900 * Longitude Start: 8.027000 * Latitude End: 64.077100 * Longitude End: 8.024000 * Date/Time Start: 2017-07-23T07:40:00 * Date/Time End: 2017-07-23T12:07:00 * Elevation Start: -293.0 m * Elevation End: -267.0 m * Location: Sula reef * Campaign: GS2017110 * Basis: G. O. Sars (2003) * Method/Device: Remote operated vehicle (ROV) * Comment: From base and across the reef. Trawl bag and lines at 10.22.
GS2017110-23-ROV12  * Latitude Start: 73.790500 * Longitude Start: 6.853160 * Latitude End: 73.770480 * Longitude End: 6.973800 * Date/Time Start: 2017-07-28T23:16:00 * Date/Time End: 2017-07-29T07:39:00 * Elevation: -2959.0 m * Location: Schultz Bank * Campaign: GS2017110 * Basis: G. O. Sars (2003) * Method/Device: Remote operated vehicle (ROV) * Comment: Northern slope of Schultz. Start on sedimentary plain at approx 3000 m
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 Event labelEventKoutsouveli, Vasiliki
2 Latitude of eventLatitudeKoutsouveli, Vasiliki
3 Longitude of eventLongitudeKoutsouveli, Vasiliki
4 Sample code/labelSample labelKoutsouveli, Vasiliki
5 SpeciesSpeciesKoutsouveli, Vasiliki
6 InvestigatorInvestigatorKoutsouveli, Vasilikiperson identifying species
7 SexSexKoutsouveli, Vasilikior non-reproductive status
8 DATE/TIMEDate/TimeKoutsouveli, VasilikiGeocode
9 Device typeDeviceKoutsouveli, Vasiliki
10 Area/localityAreaKoutsouveli, Vasiliki
11 Depth, bathymetricBathy depthmKoutsouveli, Vasilikiwater depth or range min
12 Depth, bathymetricBathy depthmKoutsouveli, Vasilikirange max
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
245 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Event		2 
Latitude		3 
Longitude		4 
Sample label		5 
Species		6 
Investigator
(person identifying species)		7 
Sex
(or non-reproductive status)		8 
Date/Time		9 
Device		10 
Area		11 
Bathy depth [m]
(water depth or range min)		12 
Bathy depth [m]
(range max)
GS2016109A-06-ROV-01 73.81577.51922.1 G. hentscheliGeogia hentscheliHans Tore RappFemale2016-06-21ROVSchulz Bank640
GS2016109A-06-ROV-0173.81577.51923.1 G. hentscheliGeogia hentscheliHans Tore RappNon-reproductive2016-06-21ROVSchulz Bank640
GS2016109A-06-ROV-0173.81577.51924.1 G. hentscheliGeogia hentscheliHans Tore RappFemale2016-06-21ROVSchulz Bank640
GS2016109A-06-ROV-0173.81577.51925.1 G. hentscheliGeogia hentscheliHans Tore RappMale2016-06-21ROVSchulz Bank640
GS2016109A-06-ROV-0173.81577.51926.1 G. hentscheliGeogia hentscheliHans Tore RappFemale2016-06-21ROVSchulz Bank640
GS2017110-23-ROV12 73.79056.8532ROV_12_11.1 G. hentscheliGeogia hentscheliHans Tore RappMale2017-07-29ROVSchulz Bank2959
GS2017110-23-ROV1273.79056.8532ROV_12_11.2 G. hentscheliGeogia hentscheliHans Tore RappFemale2017-07-29ROVSchulz Bank2959
GS2017110-23-ROV1273.79056.8532ROV_12_11.3 G. hentscheliGeogia hentscheliHans Tore RappNon-reproductive2017-07-29ROVSchulz Bank2959
GS2017110-09-ROV-6 64.07498.0270ROV6_#4_G.phlegraeiGeodia phlegraeiHans Tore RappMale2017-07-23ROVSula reef267293
GS2017110-09-ROV-664.07498.0270ROV6_#6_G.phlegraeiGeodia phlegraeiHans Tore RappFemale2017-07-23ROVSula reef267293
GS2017110-09-ROV-664.07498.0270ROV6_#7_G.phlegraeiGeodia phlegraeiHans Tore RappNon-reproductive2017-07-23ROVSula reef267293
GS2017110-57-AGT-01 71.587021.3750trawl_#9_G.phlegraeiGeodia phlegraeiHans Tore RappFemale2017-03-08trawlTromsøflaket, western Barents Sea333
GS2017110-57-AGT-0171.587021.3750trawl_#10_G.phlegraeiGeodia phlegraeiHans Tore RappMale2017-03-08trawlTromsøflaket, western Barents Sea333
GS2017110-57-AGT-0171.587021.3750trawl_#11_G.phlegraeiGeodia phlegraeiHans Tore RappMale2017-03-08trawlTromsøflaket, western Barents Sea333
GS2017110-57-AGT-0171.587021.3750trawl_#12_G.phlegraeiGeodia phlegraeiHans Tore RappMale2017-03-08trawlTromsøflaket, western Barents Sea333
Nereus20160405_ROV 58.876211.1015Gb_1Geodia barrettiPaco CárdenasNon-reproductive2016-04-05ROVKosterfjord,West of Yttre Vantenholmen, Sweden8894
Nereus20160405_ROV58.876211.1015Gb_2Geodia barrettiPaco CárdenasNon-reproductive2016-04-05ROVKosterfjord,West of Yttre Vantenholmen, Sweden8894
Nereus20160405_ROV58.876211.1015Gb_3Geodia barrettiPaco CárdenasNon-reproductive2016-04-05ROVKosterfjord,West of Yttre Vantenholmen, Sweden8894
GS2017110-09-ROV-664.07498.0270ROV6_#3_G.barrettiGeodia barrettiHans Tore RappFemale2017-07-23ROVSula reef267293
GS2017110-57-AGT-0171.587021.3750trawl_1_#5_G.barrettiGeodia barrettiHans Tore RappMale2017-03-08trawlTromsøflaket, western Barents Sea333
GS2017110-57-AGT-0171.587021.3750trawl_1_#6_G.barrettiGeodia barrettiHans Tore RappFemale2017-03-08trawlTromsøflaket, western Barents Sea333
GS2017110-57-AGT-0171.587021.3750trawl_1_#8_G.barrettiGeodia barrettiHans Tore RappMale2017-03-08trawlTromsøflaket, western Barents Sea333
HB2016944_2 59.98135.3739G.macandrewii_st2_7Geodia macandrewiiHans Tore RappFemale2016-08-09Triangular dredgeSkorpeodden, Korsfjord, Norway97
GS2017110-09-ROV-664.07498.0270ROV6_#2_G.macandrewiiGeodia macandrewiiHans Tore RappMale2017-07-23ROVSula reef267293
GS2017110-09-ROV-664.07498.0270ROV6_#10_G.macandrewiiGeodia macandrewiiHans Tore RappFemale2017-07-23ROVSula reef267293
GS2017110-57-AGT-0171.587021.3750trawl_#1_G.macandrewiiGeodia macandrewiiHans Tore RappNon-reproductive2017-03-08trawlTromsøflaket, western Barents Sea333
GS2017110-57-AGT-0171.587021.3750trawl_#2_G.macandrewiiGeodia macandrewiiHans Tore RappNon-reproductive2017-03-08trawlTromsøflaket, western Barents Sea333
GS2017110-57-AGT-0171.587021.3750trawl_#3_G.macandrewiiGeodia macandrewiiHans Tore RappNon-reproductive2017-03-08trawlTromsøflaket, western Barents Sea333
HB2016944_259.98135.3739G. atlantica_st2_8bGeodia atlanticaHans Tore RappFemale2016-08-09Triangular dredgeSkorpeodden, Korsfjord, Norway97332
HB2016944_259.98135.3739G. atlantica_st2_9bGeodia atlanticaHans Tore RappMale2016-08-09Triangular dredgeSkorpeodden, Korsfjord, Norway97332
HB2016944_259.98135.3739G. atlantica_st2_10aGeodia atlanticaHans Tore RappMale2016-08-09Triangular dredgeSkorpeodden, Korsfjord, Norway97332
HB2016952_6 59.87285.5499G.atlantica_st6_67aGeodia atlanticaHans Tore RappFemale2016-09-09Triangular dredgeLangenuen, Korsfjord, Norway23213
GS2017110-09-ROV-664.07498.0270ROV6_#1_G.atlanticaGeodia atlanticaHans Tore RappMale2017-07-23ROVSula reef267293