/* DATA DESCRIPTION: Citation: Ruiz, Micaela Belen; Taverna, Anabela; Sahade, Ricardo José; Held, Christoph (2019): Morphological and genetic data from Cnemidocarpa verrucosa sensu lato. PANGAEA, https://doi.org/10.1594/PANGAEA.909707, Supplement to: Ruiz, Micaela Belen; Taverna, Anabela; Servetto, Natalia; Sahade, Ricardo José; Held, Christoph (2020): Hidden diversity in Antarctica: Molecular and morphological evidence of two different species within one of the most conspicuous ascidian species. Ecology and Evolution, https://doi.org/10.1002/ece3.6504 Abstract: The Southern Ocean is one of the most isolated marine ecosystems, characterized by high levels of endemism, diversity and biomass. Ascidians are among the dominant groups in the Antarctic benthic communities, thus recording the evolutionary patterns of this group is crucial to improve our current understanding on the assembly of this polar ocean. In this study, we studied the genetic variation within Cnemidocarpa verrucosa sensu lato, one of the most widely distributed and abundant ascidians in Antarctica. Using a mitochondrial and a nuclear gene (mtCOI and 18S), the phylogeography of fifteen populations distributed along the Antarctic Peninsula and South America (Burdwood Bank/MPA Namuncurá) was characterized, where the bimodal distribution of the genetic distance suggested the existence of two species within the nominal C. verrucosa. When re-evaluating morphological traits to distinguish between genetically defined species, the presence of basal disc in one of the genotypes allowed to differentiate the species. Further details: 18S nuclear gene sequences (GenBank accession numbers: MN700311 - MN700622) (URI: https://store.pangaea.de/Publications/Ruiz-etal_2019/18SGenBank-AN.txt) mtCOI gene sequences (GenBank accession numbers: MN714370 - MN714622) (URI: https://store.pangaea.de/Publications/Ruiz-etal_2019/COIGenBank-AN.txt) Statistical analysis of morphology data used in Ruiz et al., 2019 (URI: https://store.pangaea.de/Publications/Ruiz-etal_2019/Statistical_analysis.docx) Coverage: LATITUDE: -62.238570 * LONGITUDE: -58.668300 MINIMUM DEPTH, water: 20 m * MAXIMUM DEPTH, water: 30 m Event(s): Carlini_Base (URI: https://en.wikipedia.org/wiki/Carlini_Base) * LATITUDE: -62.238570 * LONGITUDE: -58.668300 * ELEVATION: 5.0 m * LOCATION: Potter Peninsula, King George Island, Western Antarctica * METHOD/DEVICE: Research station (RS) * COMMENT: Ex Jubany Base Comment: (1) Both siphons are terminals, on the same line on the distal part (1) or one of them, oriented towards the one side (2). (2) Counts are made from the endostyle on the right side of the body (RE) to the dorsal lamina (DL) and from the DL to the endostyle on the left side (EL). (3) In general there are two gonadas to each side, but there are specimens with one gonad in each side, or two gonads in one side and one in other side of the body. Parameter(s): DEPTH, water [m] (Depth water) * GEOCODE * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: Scuba diving depth minimum DEPTH, water [m] (Depth water) * GEOCODE * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: Scuba diving depth maximum Sample ID (Sample ID) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: Cnemidocarpa verrucosa specimen Species, genetic (Species gen) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: Genetic determined species Color description (Color desc) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: Tunic colour Siphon, position (Siphon pos) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: Position of the siphons (1) Presence/absence (Presence/absence) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: Presence of basal disc Shape (Shape) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: Shape of warts Cnemidocarpa verrucosa, height [cm] (C. verrucosa height) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) Cnemidocarpa verrucosa, width [cm] (C. verrucosa width) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) Number (No) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: of oral tentacles Branchial formula (Branchial form) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: (2) Cnemidocarpa verrucosa, number of gonads [#] (C. verrucosa gonads) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) * COMMENT: (3) Cnemidocarpa verrucosa, number of stomach folds [#] (C. verrucosa stomach folds) * PI: Ruiz, Micaela Belen (https://orcid.org/0000-0003-1278-8248, micaruiz90@gmail.com) License: Creative Commons Attribution 4.0 International (CC-BY-4.0) (URI: https://creativecommons.org/licenses/by/4.0/) Size: 288 data points */ Depth water [m] (Scuba diving depth minimum) Depth water [m] (Scuba diving depth maximum) Sample ID (Cnemidocarpa verrucosa specimen) Species gen (Genetic determined species) Color desc (Tunic colour) Siphon pos (Position of the siphons (1)) Presence/absence (Presence of basal disc) Shape (Shape of warts) C. verrucosa height [cm] C. verrucosa width [cm] No (of oral tentacles) Branchial form ((2)) C. verrucosa gonads [#] ((3)) C. verrucosa stomach folds [#] 20 30 Cv 1 A Brown 1 Absent rounded and smooth 9699 7710 29 RE 4 (10) 3 (12) 3 (15) 3 (17) DL (11) 4 (16) 3 (15) 3 (13) 3 LE 4 28 20 30 Cv 2 A Orange 1 Absent conic with multiple spine-like ends 8741 7247 30 RE 2 (10) 3 (13) 3 (13) 3 (13) DL (13) 3 (14) 3 (10) 3 (9) 4 LE 4 26 20 30 Cv 3 A Orange 1 Absent conic with multiple spine-like ends 8918 6786 33 RE 3 (10) 3 (11) 3 (12) 3 (15) DL (14) 3 (13) 3 (12) 3 (11) 4 LE 4 18 20 30 Cv 4 A Orange/Brown 1 Absent rounded and smooth 9871 8262 31 RE 5 (8) 4 (11) 3 (14)3 (13) DL (14) 3 (13) 4 (12) 3 (9) 3 LE 4 30 20 30 Cv 6 A Brown 1 Absent conic and smoth to ventral, smooth toward the siphons 6502 5400 36 RE 3 (10) 3 (14) 3 (13) 3 (11) DL (14) 2 (14) 2 (14) 3 (12) 2 LE 4 24 20 30 Cv 8 ? Brown 1 Absent conic and smoth in the same proportion 4683 4056 24 RE 4 (10) 3 (13) 2 (16) 3 (12) DL (14) 2 (14) 3 (12) 2 (10) 2 LE 4 25 20 30 Cv 9 B Brown 2 Present rounded and smooth 15637 7221 38 RE 3 (14) 3 (15) 3 (16) 3 (16) DL (21) 4 (18) 3 (15) 3 (11) 3 LE 4 26 20 30 Cv 11 A Orange/Brown 1 Absent conic with multiple spine-like ends 6467 4098 38 RE 3 (13) 3 (13) 2 (14) 3 (12) DL (11) 3 (13) 2 (15) 2 (13) 1 LE 4 26 20 30 Cv 12 B Orange/Brown 1 Present smooth to ventral, conical toward the siphons 8778 6193 27 RE 3 (10) 3 (14) 3 (17) 3 (16) DL(16) 3 (15) 3 (9) 3 (12) 2 LE 3 28 20 30 Cv 13 A Brown 2 Absent rounded and smooth 6816 4758 26 RE 5 (8) 3 (13) 3 (14) 3 (13) DL (14) 3 (12) 3 (12) 3 (7) 3 LE 4 24 20 30 Cv 14 A Orange/Brown 1 Absent rounded and smooth 9919 8142 29 RE 3 (10) 3 (13) 3 (15) 3 (16) DL (17) 3 (14) 3 (12) 3 (11) 3 LE 4 28 20 30 Cv 15 A Brown 1 Absent rounded and smooth 4981 4305 28 RE 3 (10) 4 (13) 3 (13) 2 (17) DL (13) 3 (11) 3 (15) 3 (11) 3 LE 4 25 20 30 Cv 16 B Yellow/Brown 2 Present conic with multiple spine-like ends 16600 9250 33 RE 4 (10) 4 (7) 3 (14) 4 (16) DL (16) 4 (16) 3 (12) 4 (10) 3 LE 3 30 20 30 Cv 19 B Orange/Yellow 1 Present conic with multiple spine-like ends 10885 5027 26 RE 3 (8) 4 (10) 3 (14) 3 (13) DL (13) 3 (14) 3 (14) 3 (8) 2 LE 4 19 20 30 Cv 20 B Orange/Yellow 2 Present smooth to ventral, conical toward the siphons 17617 9584 36 RE 3 (7) 3 (10) 3 (13) 4 (17) DL (16) 4 (15) 3 (12) 3 (11) 3 LE 4 26 20 30 Cv 21 A Orange 1 Absent conic with multiple spine-like ends 6756 5333 28 RE 3 (11) 4 (10) 3 (15) 4 (15) DL (14) 3 (15) 3 (7) 4 (8) 3 LE 4 23 20 30 Cv 22 B Brown 2 Present smooth to ventral, conical toward the siphons 11788 6184 31 RE 3 (10) 3 (11) 3 (10) 3 (18) DL (16) 3 (13) 3 (10) 3 (11) 3 LE 4 24 20 30 Cv 23 A Orange/Brown 1 Absent conic with multiple spine-like ends 5776 4056 30 RE 2 (12) 3 (12) 3 (12) 3 (9) DL (11) 3 (13) 3 (12) 3 (10) 3 LE 2 23 20 30 Cv 25 B Orange/Brown 2 Present smooth to ventral, conical toward the siphons 8343 4574 24 RE 3 (11) 3 (13) 3 (14) 3 (19) DL (17) 3 (15) 3 (13) 3 (9) 3 LE 2 22 20 30 Cv 28 A Brown 2 Absent rounded and smooth 10520 6790 28 RE 3 (9) 3 (12) 3 (13) 3 (13) DL (13) 3 (14) 2 (10) 3 (9) 3 LE 4 23 20 30 Cv 29 B Orange/Brown 1 Present conic to ventral, smooth toward the siphons 10435 5501 28 RE 4 (9) 3 (11) 3 (11) 3 (15) DL (12) 3 (13) 2 (9) 3 (9) 2 LE 4 22 20 30 Cv 30 B Orange/Brown 1 Present smooth to ventral, conical toward the siphons 9347 4746 32 RE 4 (10) 3 (15) 3 (15) 3 (18) DL (17) 3 (15) 3 (16) 3 (14) 2 LE 4 23 20 30 Cv 31 B Yellow/Brown 2 Present smooth to ventral, conical toward the siphons 13615 6451 30 RE 4 (9) 3 (15) 3 (11) 3 (15) DL (15) 3 (12) 3 (15) 3 (10) 3 LE 4 20 20 30 Cv 34 A Orange/Brown 1 Absent smooth to ventral, conical toward the siphons 5914 4431 30 RE 2 (12) 3 (12)3 (13) 3 (16) DL (16) 3 (14) 3 (13) 2 (10) 1 LE 4 23