Citation:

s41467-018-04463-7

Name: Seawater carbonate chemistry and carbon, oxygen and boron isotopes within Ochrosphaera neapolitana
Published: 2018
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Ochrosphaera neapolitana; Other studied parameter or process; Pelagos; Phytoplankton; Single species

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Abstract:

Ocean acidification will potentially inhibit calcification by marine organisms; however, the response of the most prolific ocean calcifiers, coccolithophores, to this perturbation remains under characterized. Here we report novel chemical constraints on the response of the widespread coccolithophore species Ochrosphaera neapolitana (O. neapolitana) to changing-CO2 conditions. We cultured this algae under three pCO2-controlled seawater pH conditions (8.05, 8.22, and 8.33). Boron isotopes within the algae's extracellular calcite plates show that this species maintains a constant pH at the calcification site, regardless of CO2-induced changes in pH of the surrounding seawater. Carbon and oxygen isotopes in the algae's calcite plates and carbon isotopes in the algae's organic matter suggest that O. neapolitana utilize carbon from a single internal dissolved inorganic carbon (DIC) pool for both calcification and photosynthesis, and that a greater proportion of dissolved CO2 relative to HCO3− enters the internal DIC pool under acidified conditions. These two observations may explain how O. neapolitana continues calcifying and photosynthesizing at a constant rate under different atmospheric-pCO2 conditions.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Ochrosphaera neapolitana; Other studied parameter or process; Pelagos; Phytoplankton; Single species

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-11-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Yiwei		study
2	Species	Species		Liu, Yiwei
3	Registration number of species	Reg spec no		Liu, Yiwei
4	Uniform resource locator/link to reference	URL ref		Liu, Yiwei		WoRMS Aphia ID
5	Identification	ID		Liu, Yiwei		tank
6	Temperature, water	Temp	°C	Liu, Yiwei
7	Temperature, water, standard deviation	Temp std dev	±	Liu, Yiwei
8	Salinity	Sal		Liu, Yiwei
9	Salinity, standard deviation	Sal std dev	±	Liu, Yiwei
10	Alkalinity, total	AT	µmol/kg	Liu, Yiwei	Potentiometric titration
11	Alkalinity, total, standard deviation	AT std dev	±	Liu, Yiwei	Potentiometric titration
12	Carbon, inorganic, dissolved	DIC	µmol/kg	Liu, Yiwei
13	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Liu, Yiwei
14	pH	pH		Liu, Yiwei	Potentiometric	NBS scale
15	pH, standard deviation	pH std dev	±	Liu, Yiwei	Potentiometric	NBS scale
16	pH	pH		Liu, Yiwei	Calculated using CO2SYS
17	pH, standard deviation	pH std dev	±	Liu, Yiwei	Calculated using CO2SYS
18	Bicarbonate ion	[HCO3]-	µmol/kg	Liu, Yiwei	Calculated using CO2SYS
19	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Liu, Yiwei	Calculated using CO2SYS
20	Carbonate ion	[CO3]2-	µmol/kg	Liu, Yiwei	Calculated using CO2SYS
21	Carbonate ion, standard deviation	[CO3]2- std dev	±	Liu, Yiwei	Calculated using CO2SYS
22	Carbon dioxide	CO2	µmol/kg	Liu, Yiwei	Calculated using CO2SYS
23	Carbon dioxide, standard deviation	CO2 std dev	±	Liu, Yiwei	Calculated using CO2SYS
24	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Liu, Yiwei	Calculated using CO2SYS
25	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Liu, Yiwei	Calculated using CO2SYS
26	δ13C, dissolved inorganic carbon	δ13C DIC	‰ PDB	Liu, Yiwei
27	δ11B	δ11B	‰ SRM	Liu, Yiwei		coccolith
28	δ11B, standard deviation	δ11B std dev	±	Liu, Yiwei		coccolith
29	δ13C, particulate inorganic carbon	δ13C PIC	‰	Liu, Yiwei
30	Difference	Diff		Liu, Yiwei		d13C_PIC-d13C_DIC
31	Difference, standard deviation	Diff std dev	±	Liu, Yiwei		d13C_PIC-d13C_DIC
32	δ18O	δ18O	‰	Liu, Yiwei		PIC
33	Difference	Diff		Liu, Yiwei		d18O_PIC-d18O_seawater
34	Difference, standard deviation	Diff std dev	±	Liu, Yiwei		d18O_PIC-d18O_seawater
35	δ13C, particulate organic carbon	δ13C POC	‰ PDB	Liu, Yiwei
36	Difference	Diff		Liu, Yiwei		d13C_POC-d13C_DIC
37	Difference, standard deviation	Diff std dev	±	Liu, Yiwei		d13C_POC-d13C_DIC
38	Carbon, organic, particulate	POC	%	Liu, Yiwei
39	Carbon, organic, particulate, standard deviation	POC std dev	±	Liu, Yiwei
40	Carbon	C	µg	Liu, Yiwei		total
41	Carbon, total, standard deviation	TC std dev	±	Liu, Yiwei
42	Carbon, inorganic, particulate	PIC	%	Liu, Yiwei
43	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Liu, Yiwei
44	pH	pH		Liu, Yiwei		coccolith vesicle
45	pH, standard deviation	pH std dev	±	Liu, Yiwei		coccolith vesicle
46	Difference	Diff		Liu, Yiwei		coccolith vesicle pH-seawater pH
47	Difference, standard deviation	Diff std dev	±	Liu, Yiwei		coccolith vesicle pH-seawater pH
48	δ11B	δ11B	‰ SRM	Liu, Yiwei		seawater
49	δ11B, standard deviation	δ11B std dev	±	Liu, Yiwei		seawater
50	δ18O	δ18O	‰	Liu, Yiwei		seawater, VSMOW
51	δ18O	δ18O	‰	Liu, Yiwei	Mass spectrometry	seawater, VPDB
52	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
53	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
54	pH, standard deviation	pH std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)	total scale
55	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
56	Carbon dioxide, standard deviation	CO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
57	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
58	Fugacity of carbon dioxide in seawater, standard deviation	fCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
59	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
60	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
61	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
62	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
63	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
64	Carbonate ion, standard deviation	[CO3]2- std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
65	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
66	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)
67	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
68	Calcite saturation state, standard deviation	Omega Cal std dev	±	Yang, Yan	Calculated using seacarb after Orr et al. (2018)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

605 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 ID (tank)	6 Temp [°C]	7 Temp std dev [±]	8 Sal	9 Sal std dev [±]	10 AT [µmol/kg] (Potentiometric titration)	11 AT std dev [±] (Potentiometric titration)	12 DIC [µmol/kg]	13 DIC std dev [±]	14 pH (NBS scale, Potentiometric)	15 pH std dev [±] (NBS scale, Potentiometric)	16 pH (Calculated using CO2SYS)	17 pH std dev [±] (Calculated using CO2SYS)	18 [HCO3]- [µmol/kg] (Calculated using CO2SYS)	19 [HCO3]- std dev [±] (Calculated using CO2SYS)	20 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	21 [CO3]2- std dev [±] (Calculated using CO2SYS)	22 CO2 [µmol/kg] (Calculated using CO2SYS)	23 CO2 std dev [±] (Calculated using CO2SYS)	24 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	25 pCO2 std dev [±] (Calculated using CO2SYS)	26 δ13C DIC [‰ PDB]	27 δ11B [‰ SRM] (coccolith)	28 δ11B std dev [±] (coccolith)	29 δ13C PIC [‰]	30 Diff (d13C_PIC-d13C_DIC)	31 Diff std dev [±] (d13C_PIC-d13C_DIC)	32 δ18O [‰] (PIC)	33 Diff (d18O_PIC-d18O_seawater)	34 Diff std dev [±] (d18O_PIC-d18O_seawater)	35 δ13C POC [‰ PDB]	36 Diff (d13C_POC-d13C_DIC)	37 Diff std dev [±] (d13C_POC-d13C_DIC)	38 POC [%]	39 POC std dev [±]	40 C [µg] (total)	41 TC std dev [±]	42 PIC [%]	43 PIC/POC	44 pH (coccolith vesicle)	45 pH std dev [±] (coccolith vesicle)	46 Diff (coccolith vesicle pH-seawater pH)	47 Diff std dev [±] (coccolith vesicle pH-seawater pH)	48 δ11B [‰ SRM] (seawater)	49 δ11B std dev [±] (seawater)	50 δ18O [‰] (seawater, VSMOW)	51 δ18O [‰] (seawater, VPDB, Mass spectrom...)	52 CSC flag (Calculated using seacarb afte...)	53 pH (total scale, Calculated using...)	54 pH std dev [±] (total scale, Calculated using...)	55 CO2 [µmol/kg] (Calculated using seacarb afte...)	56 CO2 std dev [±] (Calculated using seacarb afte...)	57 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	58 fCO2 std dev [±] (Calculated using seacarb afte...)	59 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	60 pCO2 std dev [±] (Calculated using seacarb afte...)	61 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	62 [HCO3]- std dev [±] (Calculated using seacarb afte...)	63 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	64 [CO3]2- std dev [±] (Calculated using seacarb afte...)	65 Omega Arg (Calculated using seacarb afte...)	66 Omega Arg std dev [±] (Calculated using seacarb afte...)	67 Omega Cal (Calculated using seacarb afte...)	68 Omega Cal std dev [±] (Calculated using seacarb afte...)
laboratory	Ochrosphaera neapolitana (phytoplankton)	614495	marinespecies.org	280A	24.8	0.4	35.6	0.8	3077.2	57.5	2424.9	95.5	8.3	0.2	8.3	0.0	1922.5	117.3	495.9	23.1	6.4	1.0	227.3	33.9	-28.98	-16.95	1.83	-19.07	9.90	0.03	-2.02	31.03	0.05	-44.07	-15.09	0.07	11.69	0.950	546.390	13.946			8.28	0.16	-0.05	0.18	1.12	1.13	-3.16	-33.05	15	8.37	0.11	5.85	1.98	205.59	69.74	206.25	69.96	1931.27	167.90	487.78	81.55	7.70	1.34	11.68	2.03
laboratory	Ochrosphaera neapolitana (phytoplankton)	614495	marinespecies.org	280B	24.9	0.3	35.8	1.4	3085.7	18.2	2410.6	49.7	8.3	0.2	8.3	0.0	1892.3	89.0	512.3	41.2	6.1	0.9	215.4	32.4	-28.98	-18.13	1.83	-17.24	11.75	0.01	-3.46	29.75	0.01	-41.13	-12.15	0.07	25.41	0.950	84.672	4.693			8.22	0.18	-0.12	0.20	1.49	1.13	-3.32	-33.21	15	8.38	0.06	5.51	0.94	194.37	33.60	194.99	33.71	1901.03	85.72	504.06	38.67	7.95	0.72	12.05	1.10
laboratory	Ochrosphaera neapolitana (phytoplankton)	614495	marinespecies.org	280C	24.7	1.1	35.5	0.7	3037.1	35.4	2407.4	107.1	8.3	0.2	8.3	0.1	1922.9	154.2	477.8	48.7	6.7	1.5	235.4	47.4	-28.98	-17.09	1.83	-16.53	12.45	0.02	-1.73	31.27	0.01	-38.25	-9.26	0.07	22.42	0.950	292.795	8.227	11.394	0.508	8.26	0.18	-0.06	0.21	2.22	1.13	-3.10	-33.00	15	8.35	0.12	6.05	2.20	212.14	77.59	212.82	77.84	1931.47	183.50	469.88	81.30	7.42	1.33	11.26	2.02
laboratory	Ochrosphaera neapolitana (phytoplankton)	614495	marinespecies.org	400A	24.3	0.4	35.2	0.6	3325.8	239.2	2747.5	182.8	8.2	0.2	8.2	0.1	2283.7	150.7	454.0	69.7	9.8	1.5	341.9	47.4	-6.29	-20.65	1.83	0.75	7.04	0.01	-3.23	30.19	0.02	-20.33	-14.04	0.07	26.79	0.950	199.309	4.617	14.222	0.531	8.11	0.21	-0.11	0.24	0.31	1.13	-3.54	-33.43	15	8.26	0.29	8.89	7.19	307.92	249.20	308.91	250.00	2292.85	359.70	445.76	228.15	7.04	3.62	10.70	5.50
laboratory	Ochrosphaera neapolitana (phytoplankton)	614495	marinespecies.org	400B	25.1	0.4	35.5	1.0	3340.5	245.4	2743.2	186.6	8.2	0.2	8.2	0.0	2265.4	145.2	468.3	64.2	9.5	1.0	337.2	32.3	-6.29	-22.26	1.83	2.81	9.09	0.01	-2.43	30.19	0.02	-21.99	-15.71	0.07	27.93	0.950	278.603	6.783	11.092	0.397	7.73	0.38	-0.49	0.39	1.56	1.13	-2.71	-32.62	15	8.27	0.29	8.64	7.04	305.73	249.31	306.70	250.11	2274.62	367.97	459.94	233.76	7.28	3.72	11.03	5.63
laboratory	Ochrosphaera neapolitana (phytoplankton)	614495	marinespecies.org	400C	25.0	0.5	35.7	0.8	3171.5	92.1	2615.2	62.1	8.2	0.2	8.2	0.0	2175.1	50.0	430.6	32.5	9.5	0.7	337.5	24.4	-6.29	-21.75	1.83	4.20	10.48	0.01	-1.16	31.47	0.03	-21.40	-15.11	0.07	29.95	0.950	180.744	4.845	5.491	0.183	8.21	0.18	0.01	0.19		1.13	-2.72	-32.62	15	8.25	0.11	8.67	2.66	306.44	94.35	307.43	94.65	2183.71	125.32	422.82	83.29	6.68	1.35	10.12	2.05
laboratory	Ochrosphaera neapolitana (phytoplankton)	614495	marinespecies.org	750A	24.5	0.7	35.8	1.0	3073.6	14.2	2680.0	52.5	8.0	0.2	8.0	0.0	2355.1	78.8	309.5	29.1	15.5	2.1	541.4	72.9	-14.42	-17.96	1.83	-6.22	8.20	0.07	-0.57	31.88	0.10	-31.90	-17.48	0.07	25.82	0.950	482.034	13.110	9.115	0.353	8.26	0.18	0.22	0.20	1.36	1.13	-2.53	-32.45	15	8.07	0.07	14.06	2.85	491.18	100.76	492.76	101.08	2362.96	87.16	302.98	38.37	4.77	0.65	7.24	0.98
laboratory	Ochrosphaera neapolitana (phytoplankton)	614495	marinespecies.org	750B	25.4	0.4	35.8	1.0	3134.9	12.7	2705.1	41.9	8.0	0.2	8.1	0.0	2352.7	72.4	338.0	33.1	14.4	2.1	514.4	74.4	-14.42	-15.47	1.83	-6.25	8.17	0.01	-2.78	29.73	0.02	-30.10	-15.68	0.07	20.43	0.950	752.519	19.437	16.352	0.800	8.53	0.14	0.47	0.17	-0.11	1.13	-2.60	-32.51	15	8.10	0.06	13.06	2.05	466.42	74.10	467.91	74.33	2361.06	70.29	330.98	31.33	5.23	0.55	7.92	0.84
laboratory	Ochrosphaera neapolitana (phytoplankton)	614495	marinespecies.org	750C	24.9	0.3	35.7	1.1	3090.7	19.2	2672.7	25.4	8.0	0.2	8.1	0.0	2330.5	39.0	327.8	17.7	14.3	1.0	506.3	36.3	-14.42	-19.34	1.83	-5.82	8.60	0.01	-2.80	29.63	0.01	-30.08	-15.66	0.07	33.13	0.950	144.040	3.800			8.26	0.18	0.20	0.18	-0.14	1.13	-2.52	-32.44	15	8.10	0.04	13.07	1.49	460.94	53.38	462.42	53.55	2338.60	44.36	321.02	23.13	5.07	0.44	7.68	0.66