Citation:

j.scitotenv.2018.10.399

Name: Seawater carbonate chemistry and fitness of the edible mussel Mytilus chilensis
Published: 2019-11-26
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus chilensis; Organic toxins; Other metabolic rates; Respiration; Single species; South Pacific; Temperate

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Abstract:

High latitudes are considered particularly vulnerable to ocean acidification, since they are naturally low in carbonate ions. The edible mussel Mytilus chilensis is a common calcifier inhabiting marine ecosystems of the southern Chile, where culturing of this species is concentrated and where algal blooms produced by the toxic dinoflagellate A. catenella are becoming more frequent. Juvenile Mytilus chilensis were exposed to experimental conditions simulating two environmental phenomena: pCO2 increase and the presence of paralytic shellfish toxins (PST) produced by the toxic dinoflagellate Alexandrium catenella. Individuals were exposed to two levels of pCO2: 380 μatm (control condition) and 1000 μatm (future conditions) over a period of 39 days (acclimation), followed by another period of 40 days exposure to a combination of pCO2 and PST. Both factors significantly affected most of the physiological variables measured (feeding, metabolism and scope for growth). However, these effects greatly varied over time, which can be explained by the high individual variability described for mussels exposed to different environmental conditions. Absorption efficiency was not affected by the independent effect of the toxic diet; however, the diet and pCO2 interaction affected it significantly. The inhibition of the physiological processes related with energy acquisition by diets containing PST, may negatively impact mussel fitness, which could have important consequences for both wild and cultured mussel populations, and thus, for socioeconomic development in southern Chile.

Keyword(s):

Animalia; Behaviour; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Growth/Morphology; Laboratory experiment; Mollusca; Mytilus chilensis; Organic toxins; Other metabolic rates; Respiration; Single species; South Pacific; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -41.666670 * Longitude: -73.033330

Date/Time Start: 2012-05-01T00:00:00 * Date/Time End: 2012-05-31T00:00:00

Event(s):

Huelmo_Bay * Latitude: -41.666670 * Longitude: -73.033330 * Date/Time Start: 2012-05-01T00:00:00 * Date/Time End: 2012-05-31T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-11-20.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Navarro, Jorge M		study
2	Species	Species		Navarro, Jorge M
3	Registration number of species	Reg spec no		Navarro, Jorge M
4	Uniform resource locator/link to reference	URL ref		Navarro, Jorge M		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Navarro, Jorge M
6	Diet	Diet		Navarro, Jorge M
7	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Navarro, Jorge M
8	Clearance rate	CR	ml/h	Navarro, Jorge M
9	Absorption efficiency	Absorp eff	%	Navarro, Jorge M
10	Absorption rate	Absorp	mg/h	Navarro, Jorge M
11	Oxygen uptake rate	O2 upt rate	ml/h	Navarro, Jorge M
12	Ammonia excretion	NH3/[NH4]+ exc	µg/h	Navarro, Jorge M
13	Scope for growth	SFG	J/h	Navarro, Jorge M
14	Temperature, water	Temp	°C	Navarro, Jorge M
15	pH	pH		Navarro, Jorge M	Potentiometric	total scale
16	pH, standard deviation	pH std dev	±	Navarro, Jorge M	Potentiometric	total scale
17	Salinity	Sal		Navarro, Jorge M
18	Salinity, standard deviation	Sal std dev	±	Navarro, Jorge M
19	Alkalinity, total	AT	µmol/kg	Navarro, Jorge M	Potentiometric titration
20	Alkalinity, total, standard deviation	AT std dev	±	Navarro, Jorge M	Potentiometric titration
21	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Navarro, Jorge M	Calculated using CO2SYS
22	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Navarro, Jorge M	Calculated using CO2SYS
23	Carbonate ion	[CO3]2-	µmol/kg	Navarro, Jorge M	Calculated using CO2SYS
24	Carbonate ion, standard deviation	[CO3]2- std dev	±	Navarro, Jorge M	Calculated using CO2SYS
25	Calcite saturation state	Omega Cal		Navarro, Jorge M	Calculated using CO2SYS
26	Calcite saturation state, standard deviation	Omega Cal std dev	±	Navarro, Jorge M	Calculated using CO2SYS
27	Aragonite saturation state	Omega Arg		Navarro, Jorge M	Calculated using CO2SYS
28	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Navarro, Jorge M	Calculated using CO2SYS
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3700 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Diet	7 pCO2water_SST_wet [µatm]	8 CR [ml/h]	9 Absorp eff [%]	10 Absorp [mg/h]	11 O2 upt rate [ml/h]	12 NH3/[NH4]+ exc [µg/h]	13 SFG [J/h]	14 Temp [°C]	15 pH (total scale, Potentiometric)	16 pH std dev [±] (total scale, Potentiometric)	17 Sal	18 Sal std dev [±]	19 AT [µmol/kg] (Potentiometric titration)	20 AT std dev [±] (Potentiometric titration)	21 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	22 pCO2 std dev [±] (Calculated using CO2SYS)	23 [CO3]2- [µmol/kg] (Calculated using CO2SYS)	24 [CO3]2- std dev [±] (Calculated using CO2SYS)	25 Omega Cal (Calculated using CO2SYS)	26 Omega Cal std dev [±] (Calculated using CO2SYS)	27 Omega Arg (Calculated using CO2SYS)	28 Omega Arg std dev [±] (Calculated using CO2SYS)	29 CSC flag (Calculated using seacarb afte...)	30 CO2 [µmol/kg] (Calculated using seacarb afte...)	31 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	32 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	33 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	34 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	35 DIC [µmol/kg] (Calculated using seacarb afte...)	36 Omega Arg (Calculated using seacarb afte...)	37 Omega Cal (Calculated using seacarb afte...)
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Toxic	380	450.00	74.57	0.39	0.10	33.29	5.40	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Toxic	380	200.00	82.90	0.19	0.09	26.22	1.50	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Toxic	380	240.00	75.85	0.22	0.09	40.99	1.69	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Toxic	380	550.00	87.41	0.56	0.09	19.48	9.57	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Toxic	380	360.00	52.12	0.22	0.09	45.60	1.61	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Control	380	960.00	78.22	0.03	0.06	13.70	4.61	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Control	380	1000.00	85.58	0.05	0.05	14.56	4.61	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Control	380	720.00	89.38	0.28	0.06	13.81	4.39	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Control	380	1070.00	86.60	0.26	0.09	14.13	3.41	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Control	380	1000.00	79.07	0.37	0.09	0.00	6.03	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Toxic	380	390.00	71.63	0.82	0.11	27.29	14.24	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Toxic	380	70.00	75.67	0.62	0.06	25.80	11.27	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Toxic	380	670.00	84.85	1.04	0.08	22.58	19.59	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Toxic	380	290.00	75.61	0.87	0.11	21.62	15.60	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Toxic	380	520.00	78.78	0.87	0.09	24.32	15.83	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Control	380	810.00	78.70	0.37	0.09	14.77	5.51	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Control	380	770.00	78.74	0.89	0.07	14.13	17.12	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Control	380	610.00	82.40	1.02	0.09	15.95	19.23	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Control	380	820.00	75.11	0.69	0.10	0.00	12.62	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Control	380	860.00	72.32	0.71	0.09	11.21	12.92	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Toxic	380	420.00	87.45	0.39	0.10	22.48	5.82	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Toxic	380	690.00	80.61	0.06	0.12	23.87	7.29	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Toxic	380	620.00	85.75	0.67	0.10	15.31	11.67	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Toxic	380	650.00	82.67	0.28	0.07	34.68	3.70	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Toxic	380	470.00	79.89	0.48	0.11	0.00	7.95	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Control	380	670.00	80.01	0.20	0.07	20.02	2.25	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Control	380	580.00	74.65	0.36	0.07	14.34	5.93	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Control	380	630.00	88.38	0.72	0.10	17.13	12.72	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Control	380	750.00	79.21	0.84	0.11	24.62	14.95	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Control	380	740.00	73.79	0.39	0.10	24.62	5.55	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Toxic	380	650.00	81.62	0.81	0.10	24.94	14.41	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Toxic	380	170.00	83.26	0.66	0.09	18.30	11.56	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Toxic	380	730.00	83.75	0.89	0.11	23.55	15.95	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Toxic	380	470.00	69.46	0.78	0.11	33.18	13.43	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Toxic	380	660.00	81.18	0.85	0.10	24.99	15.18	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Control	380	800.00	61.29	0.63	0.12	26.22	10.23	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Control	380	530.00	75.31	0.49	0.08	26.33	8.17	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Control	380	50.00	52.37	0.52	0.10	22.80	8.25	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Control	380	670.00	62.34	0.52	0.14	23.55	7.70	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Control	380	580.00	65.36	0.58	0.11	24.72	9.47	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Toxic	380	660.00	81.47	0.40	0.10	51.16	5.19	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Toxic	380	760.00	79.79	0.64	0.09	41.96	10.50	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Toxic	380	460.00	76.36	0.55	0.05	38.21	9.59	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Toxic	380	630.00	79.99	0.61	0.08	39.60	10.17	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Toxic	380	760.00	81.34	0.45	0.07	23.65	7.36	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Control	380	650.00	75.04	0.59	0.11	35.43	9.47	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Control	380	830.00	84.48	0.57	0.09	24.83	9.49	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Control	380	720.00	76.78	0.61	0.09	40.57	10.07	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Control	380	680.00	70.71	0.48	0.11	36.71	7.00	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Control	380	570.00	81.53	0.34	0.11	38.85	4.01	14.00	8.06	0.03	31.79	1.88	2156.00	86.00	371.00	23.00	129.00	14.00	3.10	0.30	2.00	0.20	8	14.49	368.52	369.87	1813.41	137.93	1965.83	2.14	3.36
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Toxic	1000	300.00	82.96	0.78	0.10	30.08	12.43	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Toxic	1000	310.00	80.48	0.77	0.10	31.47	11.10	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Toxic	1000	270.00	86.72	0.76	0.09	27.89	13.48	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Toxic	1000	260.00	87.75	0.76	0.09	29.70	13.50	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Toxic	1000	410.00	76.93	0.79	0.08	30.10	11.65	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Control	1000	930.00	82.45	0.79	0.06	24.30	12.99	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Control	1000	990.00	83.45	0.85	0.09	26.78	15.20	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Control	1000	1080.00	85.53	0.83	0.06	24.62	13.60	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Control	1000	800.00	87.45	0.85	0.10	36.71	14.02	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	40	Control	1000	860.00	88.74	0.83	0.06	25.02	12.18	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Toxic	1000	620.00	63.09	0.45	0.09	22.80	7.13	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Toxic	1000	420.00	63.15	0.12	0.06	23.67	1.03	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Toxic	1000	700.00	63.34	0.54	0.08	29.87	4.60	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Toxic	1000	910.00	69.12	0.38	0.12	21.69	5.16	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Toxic	1000	450.00	63.11	0.35	0.09	22.98	5.11	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Control	1000	790.00	66.94	0.39	0.06	36.38	6.30	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Control	1000	750.00	67.57	0.16	0.04	36.37	1.78	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Control	1000	760.00	61.68	0.46	0.08	38.75	7.29	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Control	1000	690.00	71.53	0.28	0.09	39.32	3.90	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	41	Control	1000	750.00	66.92	0.11	0.06	32.47	0.24	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Toxic	1000	680.00	65.60	0.58	0.07	14.90	10.48	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Toxic	1000	580.00	63.31	0.37	0.09	11.27	5.63	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Toxic	1000	680.00	53.08	0.03	0.07	14.70	7.33	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Toxic	1000	590.00	60.42	0.52	0.10	15.85	8.79	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Toxic	1000	630.00	40.18	0.30	0.08	17.20	4.42	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Control	1000	730.00	77.48	0.66	0.09	19.55	11.50	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Control	1000	760.00	67.57	0.66	0.09	29.05	11.52	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Control	1000	730.00	78.38	0.71	0.08	28.19	12.59	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Control	1000	770.00	74.23	0.53	0.07	34.83	12.12	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	50	Control	1000	660.00	73.43	0.72	0.08	33.65	12.89	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Toxic	1000	620.00	79.42	0.58	0.06	19.34	10.50	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Toxic	1000	200.00	79.76	0.65	0.06	27.20	12.10	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Toxic	1000	680.00	76.08	0.60	0.06	21.12	6.82	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Toxic	1000	680.00	77.78	0.54	0.06	22.80	9.89	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Toxic	1000	550.00	77.74	0.63	0.06	22.50	11.63	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Control	1000	750.00	87.44	0.75	0.06	45.00	12.83	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Control	1000	750.00	87.13	0.92	0.07	32.07	17.16	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Control	1000	700.00	81.26	0.81	0.06	74.46	13.43	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Control	1000	720.00	85.76	0.76	0.07	25.30	12.80	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	60	Control	1000	870.00	87.21	0.81	0.06	48.27	10.95	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Toxic	1000	790.00	68.64	0.48	0.04	18.21	8.99	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Toxic	1000	970.00	75.27	0.76	0.06	14.49	14.29	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Toxic	1000	830.00	61.95	0.58	0.06	17.40	10.72	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Toxic	1000	740.00	68.60	0.52	0.06	18.20	9.51	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Toxic	1000	830.00	68.61	0.58	0.06	18.33	7.13	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Control	1000	650.00	78.12	0.60	0.08	47.90	10.28	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Control	1000	650.00	79.45	0.61	0.06	53.41	9.32	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Control	1000	630.00	79.02	0.57	0.08	46.06	9.21	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Control	1000	680.00	78.96	0.61	0.08	48.75	9.54	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50
laboratory	Mytilus chilensis (mollusk)	397041	marinespecies.org	80	Control	1000	780.00	72.48	0.65	0.08	43.40	11.13	14.00	7.67	0.03	31.87	1.87	2142.00	93.00	977.00	60.00	58.00	7.00	1.40	0.20	0.90	0.10	8	38.97	991.54	995.18	1988.33	61.73	2089.03	0.96	1.50