Nardi, Alessandro; Benedetti, Maura; d'Errico, Giuseppe; Fattorini, Daniele; Regoli, Francesco (2018): Seawater carbonate chemistry and accumulation and cellular responsiveness to cadmium in mussels Mytilus galloprovincialis [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.908836, Supplement to: Nardi, A et al. (2018): Effects of ocean warming and acidification on accumulation and cellular responsiveness to cadmium in mussels Mytilus galloprovincialis: Importance of the seasonal status. Aquatic Toxicology, 204, 171-179, https://doi.org/10.1016/j.aquatox.2018.09.009
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Abstract:
Ocean warming and acidification could represent an additional threat to marine organisms already coping with other anthropogenic impacts, such as chemical contamination in coastal areas. In this study, interactions between such multiple stressors and their synergistic effects in terms of accumulation, detoxification and biological effects of metals were investigated in the Mediterranean mussel Mytilus galloprovincialis. Organisms sampled during the winter period were exposed for 28 days to different combinations of two temperatures (10 °C and 15 °C), two pH/pCO2 (8.20/ 400µatm and 7.4/3000µatm) and two cadmium concentrations (0 and 20 µg/L). Cadmium concentrations increased in digestive glands and gills of metal-exposed mussels and were further enhanced by co-exposure at higher temperature. Interactive effects of temperature and/or pH were observed on Cd-mediated metallothionein induction, responsiveness of antioxidant system and onset of oxidative damages in lipids, with tissue-specific effects. Immunological effects showed a generalized sensitivity of lysosomal membrane stability toward the investigated stressors with major effects in co-exposed organisms. Cadmium and temperature affected phagocytosis efficiency and composition of haemocyte populations probably influencing the micronucleus frequency through varied mitotic rate. Several differences were highlighted between these results and those previously obtained from mussels exposed in summer, supporting the importance of season when addressing the tolerance of temperate organisms to variations of environmental factors. The elaboration of the whole biomarker results through weighted criteria allowed to summarize specific hazard indices, highlighting tissue-specific sensitivity toward multiple stressors and the need of improving the knowledge on interactions between multiple stressors.
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Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb
Project(s):
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-11-20.
Parameter(s):
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
712 data points
Data
1 Type (study) | 2 Species | 3 Reg spec no | 4 URL ref (WoRMS Aphia ID) | 5 Treat | 6 Sample label | 7 Exp duration [days] | 8 Sal | 9 Sal std dev [±] | 10 Temp [°C] | 11 Temp std dev [±] | 12 pH (NBS scale) | 13 pH std dev [±] (NBS scale) | 14 AT [µmol/kg] | 15 AT std dev [±] | 16 pCO2water_SST_wet [µatm] | 17 pCO2 std dev [±] | 18 Omega Cal | 19 Omega Cal std dev [±] | 20 Omega Arg | 21 Omega Arg std dev [±] | 22 Cd [µg/g] (in digestive gland) | 23 Cd std dev [±] (in digestive gland) | 24 MT/prot [nmol/mg] (in digestive gland) | 25 MT std dev [±] (in digestive gland) | 26 TGSH [µmol/g] (in digestive gland) | 27 TGSH std dev [±] (in digestive gland) | 28 CAT/prot [µmol/min/mg] (in digestive gland) | 29 CAT std dev [±] (in digestive gland) | 30 GST/prot [nmol/min/mg] (in digestive gland) | 31 GST std dev [±] (in digestive gland) | 32 GR/prot [nmol/min/mg] (in digestive gland) | 33 GR std dev [±] (in digestive gland) | 34 GPxSe-Dep/prot [nmol/min/mg] (in digestive gland) | 35 GPxSe-Dep std dev [±] (in digestive gland) | 36 GPxTOT/prot [nmol/min/mg] (in digestive gland) | 37 GPxTOT std dev [±] (in digestive gland) | 38 TOSC-ROO [U/mg] (in digestive gland) | 39 TOSC-ROO std dev [±] (in digestive gland) | 40 TOSC-HO [U/mg] (in digestive gland) | 41 TOSC-HO std dev [±] (in digestive gland) | 42 MDA [nmol/g] (in digestive gland) | 43 MDA std dev [±] (in digestive gland) | 44 Lipo [intensity/µm2] (in digestive gland) | 45 Lipo std dev [±] (in digestive gland) | 46 NL [intensity/µm2] (in digestive gland) | 47 NL std dev [±] (in digestive gland) | 48 Cd [µg/g] (in gills) | 49 Cd std dev [±] (in gills) | 50 MT/prot [nmol/mg] (in gills) | 51 MT std dev [±] (in gills) | 52 TGSH [µmol/g] (in gills) | 53 TGSH std dev [±] (in gills) | 54 CAT/prot [µmol/min/mg] (in gills) | 55 CAT std dev [±] (in gills) | 56 GST/prot [nmol/min/mg] (in gills) | 57 GST std dev [±] (in gills) | 58 GR/prot [nmol/min/mg] (in gills) | 59 GR std dev [±] (in gills) | 60 GPxSe-Dep/prot [nmol/min/mg] (in gills) | 61 GPxSe-Dep std dev [±] (in gills) | 62 GPxTOT/prot [nmol/min/mg] (in gills) | 63 GPxTOT std dev [±] (in gills) | 64 TOSC-ROO [U/mg] (in gills) | 65 TOSC-ROO std dev [±] (in gills) | 66 TOSC-HO [U/mg] (in gills) | 67 TOSC-HO std dev [±] (in gills) | 68 MDA [nmol/g] (in gills) | 69 MDA std dev [±] (in gills) | 70 LMS [min] (in gills) | 71 LMS std dev [±] (in gills) | 72 Phagocytosis rate [%] (Haemocytes) | 73 Phagocytosis rate std e [±] (Haemocytes) | 74 G/H | 75 G/H std dev [±] | 76 DNA damage [%] | 77 DNA damage std dev [±] | 78 Hem MN [‰] | 79 Hem MN std dev [±] | 80 CSC flag | 81 pH (total scale) | 82 CO2 [µmol/kg] | 83 fCO2water_SST_wet [µatm] | 84 pCO2water_SST_wet [µatm] | 85 [HCO3]- [µmol/kg] | 86 [CO3]2- [µmol/kg] | 87 DIC [µmol/kg] | 88 Omega Arg | 89 Omega Cal |
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Laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | CONTROL | CTRL | 28 | 37 | 0.5 | 9.95 | 0.11 | 8.18 | 0.03 | 3283.2 | 8.8 | 368.9 | 26.2 | 6.2 | 0.5 | 3.9 | 0.3 | 0.52 | 0.07 | 5.78 | 1.72 | 0.96 | 0.06 | 20.46 | 7.25 | 120.06 | 19.35 | 20.69 | 4.94 | 2.09 | 0.65 | 5.04 | 1.27 | 296.92 | 36.66 | 236.89 | 29.57 | 55.98 | 8.43 | 30.29 | 5.95 | 82.61 | 24.76 | 0.22 | 0.09 | 5.00 | 0.62 | 0.16 | 0.02 | 18.05 | 3.98 | 448.99 | 22.09 | 11.03 | 5.82 | 0.56 | 0.62 | 9.89 | 4.56 | 525.48 | 57.27 | 455.66 | 97.32 | 49.26 | 21.54 | 84.02 | 2.57 | 10.06 | 1.64 | 63.67 | 7.36 | 28.03 | 4.62 | 0.62 | 0.62 | 26 | 8.08 | 22.42 | 515.96 | 517.95 | 2779.37 | 213.70 | 3015.49 | 3.21 | 5.03 |
Laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | CONTROL + Cd | Cd | 28 | 37 | 0.5 | 9.97 | 0.06 | 8.16 | 0.03 | 3334.2 | 102.6 | 411.8 | 35.8 | 6.1 | 0.5 | 3.9 | 0.2 | 16.40 | 3.32 | 7.05 | 1.23 | 0.80 | 0.27 | 28.10 | 10.56 | 156.42 | 8.40 | 19.13 | 2.12 | 3.35 | 0.75 | 16.36 | 1.47 | 410.47 | 108.82 | 254.77 | 54.89 | 65.20 | 20.44 | 60.38 | 15.13 | 113.67 | 10.54 | 6.79 | 1.93 | 6.77 | 0.78 | 0.19 | 0.04 | 12.80 | 9.61 | 289.25 | 43.77 | 25.32 | 9.95 | 4.62 | 2.87 | 11.10 | 5.72 | 496.67 | 46.73 | 627.61 | 110.88 | 37.77 | 12.89 | 67.24 | 5.53 | 14.87 | 3.30 | 71.33 | 10.91 | 21.69 | 5.91 | 4.24 | 1.26 | 26 | 8.06 | 24.00 | 552.74 | 554.88 | 2842.85 | 208.89 | 3075.74 | 3.13 | 4.91 |
Laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | REDUCED pH | A | 28 | 37 | 0.5 | 10.54 | 0.08 | 7.40 | 0.05 | 3364.1 | 112.9 | 2882.2 | 363.8 | 1.3 | 0.2 | 0.8 | 0.1 | 0.52 | 0.07 | 5.87 | 1.29 | 0.91 | 0.11 | 25.93 | 11.60 | 129.48 | 2.47 | 11.78 | 3.17 | 2.22 | 0.50 | 12.09 | 0.65 | 233.14 | 36.38 | 233.74 | 55.62 | 42.42 | 6.69 | 30.22 | 11.95 | 74.33 | 16.78 | 0.22 | 0.09 | 4.21 | 0.13 | 0.14 | 0.03 | 16.52 | 3.65 | 429.80 | 17.66 | 12.57 | 15.55 | 0.38 | 0.06 | 20.27 | 5.93 | 554.02 | 140.13 | 412.93 | 98.26 | 40.72 | 16.40 | 58.38 | 2.91 | 8.26 | 0.39 | 71.33 | 5.36 | 36.01 | 10.64 | 1.63 | 0.52 | 26 | 7.30 | 156.76 | 3678.59 | 3692.76 | 3264.23 | 42.49 | 3463.49 | 0.64 | 1.00 |
Laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | HIGHER TEMPERATURE | W | 28 | 37 | 0.5 | 14.95 | 0.12 | 8.17 | 0.03 | 3378.4 | 121.2 | 416.8 | 34.5 | 7.2 | 0.5 | 4.7 | 0.4 | 0.52 | 0.04 | 6.76 | 1.89 | 0.73 | 0.09 | 11.14 | 1.95 | 122.41 | 2.16 | 9.59 | 1.24 | 3.26 | 0.45 | 4.86 | 1.22 | 288.18 | 29.79 | 184.59 | 19.47 | 21.35 | 2.81 | 21.71 | 4.11 | 64.25 | 17.33 | 0.22 | 0.09 | 5.89 | 1.39 | 0.15 | 0.06 | 15.45 | 2.21 | 348.00 | 73.97 | 35.70 | 7.91 | 4.24 | 0.60 | 20.89 | 5.49 | 645.31 | 25.65 | 715.86 | 35.06 | 37.48 | 17.88 | 49.19 | 6.76 | 6.80 | 0.20 | 29.04 | 4.94 | 43.89 | 12.01 | 1.00 | 0.20 | 26 | 8.06 | 20.91 | 563.77 | 565.81 | 2796.54 | 249.10 | 3066.55 | 3.77 | 5.86 |
Laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | REDUCED pH + Cd | A-Cd | 28 | 37 | 0.5 | 10.04 | 0.15 | 7.39 | 0.02 | 3360.6 | 36.8 | 2860.9 | 207.2 | 1.2 | 0.1 | 0.8 | 0.1 | 20.67 | 5.47 | 11.35 | 2.19 | 0.63 | 0.10 | 21.71 | 8.58 | 151.80 | 14.30 | 16.82 | 3.68 | 1.81 | 0.19 | 11.48 | 1.49 | 391.16 | 69.08 | 204.42 | 32.40 | 48.08 | 15.64 | 47.82 | 16.05 | 47.10 | 14.41 | 5.21 | 1.53 | 6.72 | 2.16 | 0.17 | 0.02 | 15.19 | 3.27 | 394.49 | 35.26 | 29.25 | 20.85 | 6.87 | 2.80 | 15.53 | 7.36 | 502.78 | 61.92 | 455.66 | 16.06 | 45.37 | 7.66 | 32.58 | 2.42 | 12.05 | 1.26 | 57.00 | 3.46 | 25.44 | 7.10 | 2.00 | 0.46 | 26 | 7.29 | 162.09 | 3741.74 | 3756.25 | 3264.58 | 40.83 | 3467.50 | 0.61 | 0.96 |
Laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | HIGHER TEMPERATURE + Cd | W-Cd | 28 | 37 | 0.5 | 15.02 | 0.11 | 8.17 | 0.03 | 3350.5 | 164.9 | 403.5 | 53.1 | 7.3 | 0.5 | 4.7 | 0.3 | 30.49 | 5.32 | 10.33 | 0.71 | 1.04 | 0.17 | 22.43 | 9.86 | 135.45 | 12.20 | 17.18 | 4.63 | 2.44 | 1.01 | 15.03 | 3.90 | 270.38 | 27.06 | 238.67 | 46.35 | 66.18 | 12.63 | 34.02 | 5.43 | 43.52 | 16.95 | 16.17 | 7.44 | 9.93 | 0.87 | 0.13 | 0.03 | 14.50 | 4.87 | 296.40 | 38.45 | 11.29 | 8.08 | 1.78 | 1.01 | 23.01 | 3.54 | 602.10 | 108.35 | 473.96 | 71.07 | 38.35 | 13.02 | 39.39 | 2.53 | 5.51 | 0.42 | 30.67 | 7.86 | 24.53 | 3.96 | 3.74 | 1.30 | 26 | 8.06 | 20.70 | 559.26 | 561.29 | 2771.78 | 247.46 | 3039.95 | 3.75 | 5.82 |
Laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | REDUCED pH + HIGHER TEMPERATURE | A-W | 28 | 37 | 0.5 | 14.98 | 0.05 | 7.39 | 0.04 | 3354.5 | 80.1 | 2916.3 | 288.7 | 1.5 | 0.2 | 1.0 | 0.1 | 0.52 | 0.07 | 7.00 | 1.05 | 0.93 | 0.09 | 17.57 | 5.38 | 114.66 | 7.19 | 12.70 | 1.64 | 1.16 | 1.50 | 5.96 | 2.11 | 371.90 | 42.95 | 194.84 | 63.77 | 32.15 | 10.91 | 18.04 | 13.80 | 59.12 | 21.82 | 0.22 | 0.09 | 4.64 | 0.27 | 0.18 | 0.03 | 15.82 | 3.50 | 247.96 | 52.45 | 28.28 | 21.65 | 8.82 | 0.39 | 14.60 | 3.78 | 434.27 | 52.19 | 482.52 | 57.99 | 33.18 | 9.15 | 38.90 | 5.18 | 10.49 | 1.97 | 49.67 | 10.09 | 18.98 | 4.76 | 2.87 | 0.94 | 26 | 7.28 | 146.23 | 3945.13 | 3959.49 | 3242.43 | 47.92 | 3436.57 | 0.73 | 1.13 |
Laboratory | Mytilus galloprovincialis (mollusk) | 140481 | marinespecies.org | REDUCED pH + HIGHER TEMPERATURE + Cd | A-W-Cd | 28 | 37 | 0.5 | 14.92 | 0.06 | 7.39 | 0.02 | 3326.5 | 67.1 | 2886.4 | 174.1 | 1.5 | 0.1 | 1.0 | 0.1 | 33.40 | 7.57 | 9.76 | 2.26 | 0.94 | 0.22 | 12.34 | 2.01 | 120.79 | 8.37 | 16.03 | 2.93 | 2.46 | 1.41 | 6.28 | 1.47 | 462.13 | 73.06 | 167.03 | 29.80 | 66.10 | 19.96 | 67.26 | 15.84 | 29.68 | 11.78 | 19.38 | 2.18 | 6.52 | 1.11 | 0.13 | 0.01 | 16.82 | 1.54 | 339.37 | 38.21 | 21.99 | 11.87 | 2.66 | 0.82 | 8.69 | 0.47 | 502.91 | 19.67 | 516.47 | 59.24 | 32.84 | 5.04 | 26.30 | 2.93 | 4.45 | 0.52 | 33.33 | 6.84 | 27.28 | 12.48 | 3.98 | 1.99 | 26 | 7.28 | 145.17 | 3909.50 | 3923.74 | 3215.44 | 47.42 | 3408.03 | 0.72 | 1.11 |