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Wu, Yu-Chen; García-Altares, María; Ribes, Marta; Hentschel, Ute; Pita, Lucía (2019): Microscopic images and 2D-MALDI-images of the sponge Aplysina aerophoba in response to grazing and video recording of grazing behavior. PANGAEA,

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We aimed to investigate the cellular and chemical response of the chemically defended sponge Aplysina aerophoba (Phylum Porifera: Class Demospongiae) to grazing by its specialist Tylodina perversa (Phylum Mollusca: Class Opistobranchia). Three treatments were applied: control, grazing, and mechanical damage. Samples were collected 3 hours, 1 day, 3 days, and 6 days after treatment. The behavior of sea slugs after directly contact with sponge specimen was recorded by using a GoPro Hero 4 camera with the program time lapse (1 picture every 5 sec) for 1.5 to 2 hours. Our results showed that spherulous cells were recruited to the wounded site in a time-dependent manner. MALDI-imaging MS showed that both brominated compounds (aerophobin-2 and aeroplysinin-1) localized usually at the sponge surface and accumulated at the damaged surface upon wounding. As spherulous cells are common in many members of the class Demospongiae, the recruitment of defensive cells may also occur in other sponges for protecting these filter-feeders. Our study contributes to understanding the evolutionary mechanisms in sponges for facing grazing and wounding.
grazing; MALDI-imaging mass spectrometry; microscopy; Porifera; response to wounding; spherulous cells; sponge; transmission electron microscopy
Supplement to:
Wu, Yu-Chen; García-Altares, María; Pintó, Berta; Ribes, Marta; Hentschel, Ute; Pita, Lucía (2020): Opisthobranch grazing results in mobilisation of spherulous cells and re-allocation of secondary metabolites in the sponge Aplysina aerophoba. Scientific Reports, 10(1),
Related to:
Wu, Yu-Chen (2019): The cellular, chemical, and molecular response of the sponge Aplysina aerophoba to grazing. urn:nbn:de:gbv:8-mods-2019-00088-1
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1ExperimentExpWu, Yu-ChenTime point-year
2TreatmentTreatWu, Yu-ChenControl/grazing/mechanical damage
3Tissue, samplingTissueWu, Yu-ChenInvestigated tissue area
4TypeTypeWu, Yu-ChenLight microscopy/TEM/2D-MALDI-image
5MagnificationMagXWu, Yu-ChenMagnification of light microscopy
6Image resolutionImage resµmWu, Yu-ChenRaster size in MALDI-imaging MS
7CompoundsCompWu, Yu-ChenMeasured brominated compounds
8ReplicateReplicateWu, Yu-ChenSponge individual, biological replicate
9CommentCommentWu, Yu-Chen
10File nameFile nameWu, Yu-Chen
11File formatFile formatWu, Yu-Chen
12File sizeFile sizekByteWu, Yu-Chen
13Uniform resource locator/link to imageURL imageWu, Yu-Chen
14Uniform resource locator/link to movieURL movieWu, Yu-Chen
994 data points

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