Citation:

fsz028

Name: Seawater carbonate chemistry and photosynthesis and dark respiration of Thalassiosira weissflogii (diatom)
Published: 2019-10-29
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Thalassiosira weissflogii

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Abstract:

Increasing atmospheric pCO2 leads to seawater acidification, which has attracted considerable attention due to its potential impact on the marine biological carbon pump and function of marine ecosystems. Alternatively, phytoplankton cells living in coastal waters might experience increased pH/decreased pCO2 (seawater alkalization) caused by metabolic activities of other photoautotrophs, or after microalgal blooms. Here we grew Thalassiosira weissflogii (diatom) at seven pCO2 levels, including habitat-related lowered levels (25, 50, 100, and 200 µatm) as well as present-day (400 µatm) and elevated (800 and 1600 µatm) levels. Effects of seawater acidification and alkalization on growth, photosynthesis, dark respiration, cell geometry, and biogenic silica content of T. weissflogii were investigated. Elevated pCO2 and associated seawater acidification had no detectable effects. However, the lowered pCO2 levels (25-100 µatm), which might be experienced by coastal diatoms in post-bloom scenarios, significantly limited growth and photosynthesis of this species. In addition, seawater alkalization resulted in more silicified cells with higher dark respiration rates. Thus, a negative correlation of biogenic silica content and growth rate was evident over the pCO2 range tested here. Taken together, seawater alkalization, rather than acidification, could have stronger effects on the ballasting efficiency and carbon export of T. weissflogii.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Growth/Morphology; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Thalassiosira weissflogii

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2019) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-10-24.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Xu, Juntian		study
2	Species	Species		Xu, Juntian
3	Registration number of species	Reg spec no		Xu, Juntian
4	Uniform resource locator/link to reference	URL ref		Xu, Juntian		WoRMS Aphia ID
5	Treatment	Treat		Xu, Juntian		pCO2
6	Replicate	Repl		Xu, Juntian
7	Growth rate	µ	1/day	Xu, Juntian		specific
8	Chlorophyll a per cell	Chl a/cell	pg/#	Xu, Juntian
9	Biogenic silica, per cell	bSiO2/cell	fmol/#	Xu, Juntian
10	Change	Change	%	Xu, Juntian		Fv/Fm
11	Change	Change	%	Xu, Juntian		alpha
12	Change	Change	%	Xu, Juntian		rETR
13	Change	Change	%	Xu, Juntian		Ik
14	Net oxygen evolution, per cell	O2 ev/cell	pmol/#/h	Xu, Juntian
15	Respiration rate, oxygen, dark per cell	Resp O2 d/cell	pmol/#/h	Xu, Juntian		dark
16	Cell biovolume	Cell biovol	µm³	Xu, Juntian
17	Surface area	SA	µm²	Xu, Juntian
18	Cell surface area/cell volume ratio	Cell SA/cell vol	1/µm	Xu, Juntian
19	Biogenic silica per surface area	bSiO2/SA	fmol/µm*2	Xu, Juntian
20	Temperature, water	Temp	°C	Xu, Juntian
21	Salinity	Sal		Xu, Juntian
22	pH	pH		Xu, Juntian		NBS scale
23	Alkalinity, total	AT	µmol/kg	Xu, Juntian
24	Carbon, inorganic, dissolved	DIC	µmol/kg	Xu, Juntian
25	Bicarbonate ion	[HCO3]-	µmol/kg	Xu, Juntian
26	Carbon dioxide	CO2	µmol/kg	Xu, Juntian
27	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
29	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

862 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat (pCO2)	6 Repl	7 µ [1/day] (specific)	8 Chl a/cell [pg/#]	9 bSiO2/cell [fmol/#]	10 Change [%] (Fv/Fm)	11 Change [%] (alpha)	12 Change [%] (rETR)	13 Change [%] (Ik)	14 O2 ev/cell [pmol/#/h]	15 Resp O2 d/cell [pmol/#/h] (dark)	16 Cell biovol [µm³]	17 SA [µm²]	18 Cell SA/cell vol [1/µm]	19 bSiO2/SA [fmol/µm*2]	20 Temp [°C]	21 Sal	22 pH (NBS scale)	23 AT [µmol/kg]	24 DIC [µmol/kg]	25 [HCO3]- [µmol/kg]	26 CO2 [µmol/kg]	27 CSC flag	28 pH (total scale)	29 CO2 [µmol/kg]	30 fCO2water_SST_wet [µatm]	31 pCO2water_SST_wet [µatm]	32 [HCO3]- [µmol/kg]	33 [CO3]2- [µmol/kg]	34 DIC [µmol/kg]	35 Omega Arg	36 Omega Cal
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	25	1	1.19	2.58	4350	10.58	4.16	-43.25	-45.60	0.80	3.22	1200.51	634.55	0.53	6.85	20	35	9.02	2382.58	1404	795	0.8	15	8.90	0.86	26.44	26.53	865.58	611.61	1478.05	9.51	14.63
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	50	1	1.22	4.07	4110	3.00	24.01	-12.78	-29.78	1.21	2.42	1423.58	704.14	0.49	5.83	20	35	8.84	2403.85	1570	1041	1.6	15	8.72	1.68	51.70	51.87	1118.06	521.95	1641.68	8.12	12.49
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	100	1	1.23	5.01	4770	2.47	17.78	17.56	-0.34	1.57	1.57	1751.16	811.95	0.46	5.87	20	35	8.63	2375.64	1727	1314	3.3	15	8.51	3.38	104.30	104.65	1390.68	400.27	1794.33	6.22	9.58
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	200	1	1.36	5.49	2380	0.18	-4.11	-3.39	0.60	2.75	1.83	1867.83	845.02	0.45	2.81	20	35	8.45	2411.07	1902	1572	6.0	15	8.33	6.05	186.73	187.37	1645.01	312.81	1963.87	4.86	7.48
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	400	1	1.56	4.75	840	2.47	10.47	10.28	-0.32	2.71	1.36	2174.66	930.77	0.43	0.90	20	35	8.16	2406.25	2093	1880	13.9	15	8.04	13.94	430.08	431.54	1942.87	189.46	2146.26	2.95	4.53
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	400*	1	1.50	4.45	900	2.86	4.71	0.00	0.08	3.07	1.02	2269.32	964.70	0.43	0.93	20	35	8.16	2371.84	2060	1851	13.7	15	8.04	13.73	423.72	425.17	1914.09	186.64	2114.46	2.90	4.47
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	800	1	1.59	4.62	870	-1.43	2.22	-16.44	-14.34	2.54	0.85	1827.09	832.00	0.46	1.04	20	35	7.93	2330.34	2137	1988	24.9	15	7.81	24.88	767.87	770.49	2042.09	117.23	2184.20	1.82	2.80
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	1600	1	1.54	4.23	840	1.43	-9.62	-7.94	6.74	2.19	0.73	1766.63	813.49	0.46	1.03	20	35	7.63	2337.64	2257	2137	53.5	15	7.51	53.11	1638.85	1644.44	2183.45	62.79	2299.35	0.98	1.50
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	25	2	0.93	3.16	6580	0.00	-6.17	-47.76	-44.41			1128.62	609.19	0.54	10.80	20	35	9.01	2344.98	1386	792	0.8	15	8.89	0.87	26.97	27.06	862.71	595.70	1459.29	9.26	14.25
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	50	2	1.27	3.88	5060	3.00	13.92	5.61	-7.43	1.49	2.99	1670.43	787.69	0.47	6.43	20	35	8.83	2360.03	1546	1033	1.6	15	8.71	1.70	52.48	52.65	1109.05	505.96	1616.71	7.87	12.10
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	100	2	1.31	4.62	3850	0.88	-5.96	-6.87	-1.12	1.78	1.78	1794.66	823.77	0.46	4.68	20	35	8.62	2384.24	1743	1333	3.4	15	8.50	3.51	108.17	108.53	1409.49	396.46	1809.46	6.16	9.48
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	200	2	1.34	5.49	2090	0.18	5.87	3.11	-2.76	2.50	1.67	1767.62	816.37	0.46	2.56	20	35	8.42	2376.30	1894	1582	6.4	15	8.30	6.52	201.32	202.01	1655.12	293.72	1955.36	4.57	7.03
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	400	2	1.61	5.83	1110	-0.71	-3.30	-5.91	-2.85	2.45	1.22	2146.13	922.74	0.43	1.20	20	35	8.19	2361.43	2034	1816	12.5	15	8.07	12.58	388.27	389.59	1879.36	196.36	2088.31	3.05	4.70
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	400*	2	1.53	5.47	1170	0.00	-2.21	-11.42	-5.07	2.50	1.25	1982.58	879.01	0.44	1.33	20	35	8.16	2359.41	2049	1841	13.6	15	8.04	13.66	421.42	422.86	1903.68	185.63	2102.97	2.89	4.44
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	800	2	1.58	6.14	570	0.00	2.62	-11.00	-9.12	2.86	1.43	1912.52	857.21	0.45	0.67	20	35	7.93	2373.16	2178	2026	25.4	15	7.81	25.35	782.30	784.96	2080.49	119.43	2225.28	1.86	2.86
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	1600	2	1.49	4.42	810	0.00	2.16	-6.47	-4.06	2.45	1.22	1929.20	861.49	0.45	0.94	20	35	7.62	2393.02	2317	2193	56.2	15	7.50	55.73	1719.81	1725.68	2239.16	62.93	2357.83	0.98	1.51
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	25	3	0.99	4.11	3900	3.35	-1.91	-40.28	-39.21	1.61	3.21	1305.00	671.08	0.51	5.81	20	35	9.03	2364.15	1384	775	0.8	15	8.91	0.82	25.23	25.32	845.22	611.13	1457.16	9.50	14.62
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	50	3	1.26	3.03	5370	1.41	7.86	-22.03	-27.82	1.21	2.42	1567.43	755.94	0.48	7.11	20	35	8.83	2364.15	1549	1036	1.6	15	8.71	1.70	52.58	52.76	1111.21	506.94	1619.85	7.88	12.13
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	100	3	1.28	4.83	4000	3.70	0.06	11.40	11.15	1.56	1.56	1657.11	782.02	0.47	5.11	20	35	8.63	2446.91	1785	1358	3.4	15	8.51	3.49	107.68	108.05	1435.89	413.30	1852.68	6.43	9.89
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	200	3	1.32	4.30	2280	-1.59	-10.74	-30.47	-22.23	2.08	1.39	1816.39	830.66	0.46	2.74	20	35	8.44	2335.42	1844	1530	5.9	15	8.32	6.03	186.08	186.72	1601.90	297.67	1905.60	4.63	7.12
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	400	3	1.56	5.88	1110	-1.76	-6.72	-3.57	3.21	2.55	1.27	2102.83	911.08	0.43	1.22	20	35	8.17	2376.53	2059	1846	13.3	15	8.05	13.39	413.06	414.47	1909.41	190.53	2113.32	2.96	4.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	400*	3	1.57	5.78	1130	-1.43	-2.05	-6.44	0.10	2.29	1.53	1971.54	877.14	0.44	1.28	20	35	8.17	2362.29	2046	1835	13.2	15	8.05	13.30	410.49	411.89	1897.53	189.34	2100.18	2.94	4.53
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	800	3	1.44	5.06	750	-1.43	-6.72	-5.23	6.47	2.89	1.44	1766.63	813.49	0.46	0.92	20	35	7.92	2394.99	2202	2051	26.3	15	7.80	26.26	810.33	813.09	2105.98	118.15	2250.39	1.84	2.83
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	1600	3	1.50	4.78	660	-1.43	3.54	-12.56	-11.50	2.01	1.34	1840.75	835.39	0.45	0.79	20	35	7.63	2366.23	2286	2165	54.2	15	7.51	53.77	1659.14	1664.80	2210.48	63.57	2327.82	0.99	1.52