Bienhold, Christina (2019): Microbial cell numbers measured during POLARSTERN cruise PS94 (TransArcII) to the Barents Sea and central Arctic [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.907884, In: Kiesel, Joshua; Bienhold, Christina; Wenzhöfer, Frank; Link, Heike (2019): Benthic biogeochemical parameters measured during Polarstern expedition PS94 (TransArcII) to the Barents Sea and central Arctic [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.907889
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Abstract:
For the determination of prokaryotic cell numbers, 1 cm³ sediment was fixed with sterile filtered formalin/seawater at a final concentration of 2% and stored at 4°C until further analyses in the home laboratory. Samples were filtered on 0.2 µm polycarbonate filters, stained with acridine orange (Meyer-Reil, 1983, doi:10.1007/bf00395813), and counted using an epifluorescence microscope (Axiophot II Imaging, Zeiss, Jena, Germany). For each sample, 30 random grids from two replicate filters were counted and averaged.
Keyword(s):
Supplement to:
Kiesel, Joshua; Bienhold, Christina; Wenzhöfer, Frank; Link, Heike (2020): Variability in Benthic Ecosystem Functioning in Arctic Shelf and Deep-Sea Sediments: Assessments by Benthic Oxygen Uptake Rates and Environmental Drivers. Frontiers in Marine Science, 7, 426, https://doi.org/10.3389/fmars.2020.00426
Project(s):
Funding:
Federal Ministry of Education and Research (BMBF), grant/award no. 03G0833E: Verbundprojekt: WTZ RUS: System Laptev-See: TRANSDRIFT
Seventh Framework Programme (FP7), grant/award no. 294757: Assessment of bacterial life and matter cycling in deep-sea surface sediments
Coverage:
Median Latitude: 81.896614 * Median Longitude: 90.178723 * South-bound Latitude: 72.734000 * West-bound Longitude: 22.826000 * North-bound Latitude: 89.916170 * East-bound Longitude: -121.750830
Date/Time Start: 2015-08-18T20:46:00 * Date/Time End: 2015-10-08T10:20:00
Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.005 m
Event(s):
PS94/001-2 * Latitude: 74.998500 * Longitude: 30.004000 * Date/Time: 2015-08-18T20:46:00 * Elevation: -372.0 m * Location: Barents Sea * Campaign: PS94 (ARK-XXIX/3) * Basis: Polarstern * Method/Device: MultiCorer (MUC)
PS94/002-2 * Latitude: 76.676330 * Longitude: 30.003830 * Date/Time: 2015-08-19T05:56:00 * Elevation: -265.0 m * Location: Barents Sea * Campaign: PS94 (ARK-XXIX/3) * Basis: Polarstern * Method/Device: MultiCorer (MUC)
PS94/021-2 * Latitude: 80.996500 * Longitude: 28.969500 * Date/Time: 2015-08-21T10:22:00 * Elevation: -389.5 m * Location: Arctic Ocean * Campaign: PS94 (ARK-XXIX/3) * Basis: Polarstern * Method/Device: MultiCorer (MUC)
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Kiesel, Joshua | |||
| 2 | Date/Time of event | Date/Time | Kiesel, Joshua | |||
| 3 | Latitude of event | Latitude | Kiesel, Joshua | |||
| 4 | Longitude of event | Longitude | Kiesel, Joshua | |||
| 5 | Elevation of event | Elevation | m | Kiesel, Joshua | ||
| 6 | DEPTH, sediment/rock | Depth sed | m | Kiesel, Joshua | Geocode | |
| 7 | Depth, top/min | Depth top | m | Kiesel, Joshua | ||
| 8 | Depth, bottom/max | Depth bot | m | Kiesel, Joshua | ||
| 9 | Prokaryotes, abundance as single cells | Prok cell abund | 109 #/cm3 | Kiesel, Joshua | Acridine Orange Direct Counting (AODC) | |
| 10 | Comment | Comment | Kiesel, Joshua |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
48 data points
Data
| 1 Event | 2 Date/Time | 3 Latitude | 4 Longitude | 5 Elevation [m] | 6 Depth sed [m] | 7 Depth top [m] | 8 Depth bot [m] | 9 Prok cell abund [109 #/cm3] | 10 Comment |
|---|---|---|---|---|---|---|---|---|---|
| PS94/001-2 | 2015-08-18T20:46 | 74.9985 | 30.0040 | -372 | 0.005 | 0 | 0.01 | 1.86 | shelf |
| PS94/002-2 | 2015-08-19T05:56 | 76.6763 | 30.0038 | -265 | 0.005 | 0 | 0.01 | 3.84 | shelf |
| PS94/021-2 | 2015-08-21T10:22 | 80.9965 | 28.9695 | -390 | 0.005 | 0 | 0.01 | 2.91 | shelf |
| PS94/032-10 | 2015-08-23T05:12 | 81.8577 | 30.8967 | -3167 | 0.005 | 0 | 0.01 | 2.21 | deep sea |
| PS94/066-2 | 2015-09-01T16:30 | 86.7102 | 61.3170 | -656 | 0.005 | 0 | 0.01 | 0.44 | deep sea |
| PS94/087-2 | 2015-09-08T07:25 | 89.9162 | -121.7508 | -4262 | 0.005 | 0 | 0.01 | 0.71 | deep sea |
| PS94/101-10 | 2015-09-14T17:46 | 87.4950 | -179.8788 | -3995 | 0.005 | 0 | 0.01 | 0.46 | deep sea |
| PS94/105-2 | 2015-09-15T23:54 | 86.9768 | 146.8102 | -1001 | 0.005 | 0 | 0.01 | 0.81 | deep sea |
| PS94/123-2 | 2015-09-21T21:19 | 85.0530 | 137.5513 | -4113 | 0.005 | 0 | 0.01 | 0.73 | deep sea |
| PS94/130-4 | 2015-09-24T01:05 | 85.0263 | 151.5902 | -866 | 0.005 | 0 | 0.01 | 1.23 | deep sea |
| PS94/149-5/6 | 2015-10-07T00:00 | 74.3189 | 23.8057 | -301 | 0.005 | 0 | 0.01 | 2.17 | shelf |
| PS94/161-6 | 2015-10-08T10:20 | 72.7340 | 22.8260 | -395 | 0.005 | 0 | 0.01 | 1.78 | shelf |