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Verse, Leander; Laudien, Jürgen; Häussermann, Verena (2019): Percentage of Primnoella chilensis polyps activity states under sediment exposure of 15 days, observation period 21 h, Treatment 2, Punta Gruesa [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.907720, In supplement to: Verse, Leander (2019): Response to sedimentation stress of the gorgonian coral Primnoella chilensis from the Chilean fjord region. Bachelor Thesis, Freie Universiät zu Berlin, 35 pp

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Abstract:
To determine behavioural stress, polyp activity states of Primnoella chilensis colonies were observed for 21 hours after eleven days under turbid seawater exposure in an experimental setup (Treatment: equals sedimentation rate of 450 mm/y; control group: ambient seawater). Image analysis was conducted by drawing a segmented selection line along the centre of the colonies' internal axis using the image-processing program ImageJ. This line was moved to the side by 175 % of the internal axis' width measured in pixels and 250 % respectively, resulting in two lines that were named selection A and B. These distances were chosen because fully expanded polyps showed a horizontal spread of about two times the internal axis' diameter and fully closed polyps less than one time respectively. For both lines, a plot profile was created showing grey values of all pixels under the segmented selection line. Lighter pixels of the extended polyps in comparison to the black background resulted in countable peaks. Therefore, the plot was divided by the highest and lowest grey values and bisected horizontally using the vector graphics editor Adobe Illustrator. The number of peaks in the upper part of the divided plot-image was then counted in ImageJ using the function 'analyze particles'. Lastly, the number of polyps of a category was calculated: category 0 (closed): = total polyps observed minus counted peaks for selection line A; category 1 (semi-open): = counted peaks for selection line A minus counted peaks for selection line B; category 2: = counted peaks for selection B.
Project(s):
Coverage:
Latitude: -42.409833 * Longitude: -72.424333
Date/Time Start: 2019-06-09T00:20:00 * Date/Time End: 2019-06-09T23:20:00
Minimum Elevation: -20.0 m * Maximum Elevation: -20.0 m
Event(s):
Punta_Gruesa_Diver * Latitude: -42.409833 * Longitude: -72.424333 * Elevation: -20.0 m * Location: Comau Fjord, Patagonia, Chile * Method/Device: Sampling by diver (DIVER)
Comment:
This dataset was generated in the frame of the co-operation between the Huinay Scientific Field Station (http://www.huinay.cl) and the Alfred Wegener Institute, Helmholtz Center for Polar and Marine Research (http://www.awi.de).
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Colony number/IDColony no/IDVerse, Leander
2TreatmentTreatVerse, Leander
3DATE/TIMEDate/TimeVerse, LeanderGeocode
4Coral polypPolyp#Verse, Leandertotal polyps measured
5Coral polypPolyp#Verse, Leanderpolyps category 0
6Coral polypPolyp%Verse, Leanderpolyps category 0
7Coral polypPolyp#Verse, Leanderpolyps category 1
8Coral polypPolyp%Verse, Leanderpolyps category 1
9Coral polypPolyp#Verse, Leanderpolyps category 2
10Coral polypPolyp%Verse, Leanderpolyps category 2
Size:
567 data points

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