Citation:

PANGAEA.903579

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Abstract:

Spring phytoplankton blooms contribute significantly to global marine primary production. A large fraction of the bloom derived organic matter is available to heterotrophic bacteria in the form of polysaccharides. We analyzed changes in the modes of polysaccharide utilization (selfish uptake and extracellular hydrolysis) during a spring phytoplankton bloom using fluorescently labelled polysaccharide incubations coupled with 16s rRNA sequencing and fluorescence in situ hybridization. We found that in the early bloom phases there was high selfish activity of simple polysaccharides (laminarin) and low extracellular hydrolysis rates of a limited range of polysaccharides. During the course of the bloom both the selfish uptake and extracellular hydrolysis rates increased but only for a limited range of substrates. At the late bloom phase a wide range of substrate was extracellularly hydrolyzed and the level of selfish uptake decreased. We found that during a spring phytoplankton bloom the mode of substrate utilization depended on both the substrates structural complexity and the composition of the heterotrophic community related to the bloom phase.

Supplement to:

Reintjes, Greta; Fuchs, Bernhard M; Scharfe, Mirco; Wiltshire, Karen Helen; Amann, Rudolf; Arnosti, Carol (2020): Short‐term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom. Environmental Microbiology, 22(5), 1884-1900, https://doi.org/10.1111/1462-2920.14971

Coverage:

Latitude: 54.188330 * Longitude: 7.900000

Date/Time Start: 2016-03-22T12:00:00 * Date/Time End: 2016-05-03T12:00:00

Minimum DEPTH, water: 5 m * Maximum DEPTH, water: 5 m

Event(s):

HelgolandRoads_site (Kabeltonne) * Latitude: 54.188330 * Longitude: 7.900000 * Elevation: -10.0 m * DEIMS ID: deims.org * Location: German Bight, North Sea * Comment: Samples and Measurements were taken at HelgolandRoads site but independently of Helgoland Roads LTER project

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Device type	Device		Reintjes, Greta
2	Sample code/label	Sample label		Reintjes, Greta
3	DEPTH, water	Depth water	m	Reintjes, Greta		Geocode
4	Substrate type	Substrate		Reintjes, Greta
5	DATE/TIME	Date/Time		Reintjes, Greta		Geocode
6	Experiment day	Exp day	day	Reintjes, Greta
7	Sample ID	Sample ID		Reintjes, Greta
8	Sample ID	Sample ID		Reintjes, Greta		ENA
9	Size	Size	µm	Reintjes, Greta
10	Volume	Vol	ml	Reintjes, Greta
11	Bacterioplankton	Bacterioplankton	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
12	Bacteroidetes, targeted with CF319a oligonucliotide FISH-Probe	CF319a	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
13	Alteromonadales, targeted with ALT1413 oligonucleiotide FISH-Probe	ALT1413	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
14	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)	of FLA-substrate stained cells
15	Bacteroidetes, substrate stained	Bacteroidetes sub stain	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
16	Alteromonadales, substrate stained	Alteromonadales sub stain	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

1448 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Device	2 Sample label	3 Depth water [m]	4 Substrate	5 Date/Time	6 Exp day [day]	7 Sample ID	8 Sample ID (ENA)	9 Size [µm]	10 Vol [ml]	11 Bacterioplankton [#/ml]	12 CF319a [#/ml]	13 ALT1413 [#/ml]	14 Microbial abund [#/ml] (of FLA-substrate stained cells)	15 Bacteroidetes sub stain [#/ml]	16 Alteromonadales sub stain [#/ml]
Incubation	HEL2016_FLA_substrate_1	5	Laminarin	2016-03-22T12:00	0	HEL_1_La_0	HEL_1_T0_La	0.2	20	6.83E5	1.30E5	3.37E2	1.00E5	4.48E4	3.37E2
Incubation	HEL2016_FLA_substrate_2	5	Laminarin	2016-03-22T12:00	1	HEL_1_La_24		0.2	20	8.33E5	2.16E5		4.57E4	4.59E4
Incubation	HEL2016_FLA_substrate_3	5	Laminarin	2016-03-22T12:00	3	HEL_1_La_72	Hel_1_T72_La	0.2	20	1.02E6	1.94E5	0.00E0	6.32E4	2.82E3	0.00E0
Incubation	HEL2016_FLA_substrate_4	5	Laminarin	2016-03-22T12:00	6	HEL_1_La_144	HEL_1_T144_La	0.2	20	1.11E6	3.64E5	4.29E3	9.09E4	2.92E3	1.79E3
Incubation	HEL2016_FLA_substrate_5	5	Laminarin	2016-03-22T12:00	7	HEL_1_La_168		0.2	20	8.99E5	1.86E5	3.18E4	2.04E4	3.11E3	4.92E3
Incubation	HEL2016_FLA_substrate_6	5	Xylan	2016-03-22T12:00	0	HEL_1_Xy_0	Hel_1_T0_Xy	0.2	20	6.58E5		8.80E2	2.37E4		8.80E2
Incubation	HEL2016_FLA_substrate_7	5	Xylan	2016-03-22T12:00	1	HEL_1_Xy_24		0.2	20	8.02E5	2.23E5	1.21E3	3.10E4	1.27E4	1.21E3
Incubation	HEL2016_FLA_substrate_8	5	Xylan	2016-03-22T12:00	3	HEL_1_Xy_72	HEL_1_T72_Xy	0.2	20	1.31E6	4.28E5	1.55E4	5.49E4	2.98E4	1.57E3
Incubation	HEL2016_FLA_substrate_9	5	Xylan	2016-03-22T12:00	6	HEL_1_Xy_144	HEL_1_T144_Xy	0.2	20	1.06E6	3.26E5	7.68E4	1.12E5	1.21E5	1.10E4
Incubation	HEL2016_FLA_substrate_10	5	Xylan	2016-03-22T12:00	7	HEL_1_Xy_168		0.2	20	8.55E5	1.42E5	9.80E4	3.35E4	7.91E3	7.09E3
Incubation	HEL2016_FLA_substrate_11	5	Chondroitin sulfate	2016-03-22T12:00	0	HEL_1_Ch_0	HEL_1_T0_Ch	0.2	20	7.76E5	1.73E5	2.09E3	1.19E4	2.38E3	2.09E3
Incubation	HEL2016_FLA_substrate_12	5	Chondroitin sulfate	2016-03-22T12:00	1	HEL_1_Ch_24		0.2	20	8.38E5	2.11E5	9.00E2	1.31E4	3.47E3	9.00E2
Incubation	HEL2016_FLA_substrate_13	5	Chondroitin sulfate	2016-03-22T12:00	3	HEL_1_Ch_72	HEL_1_T72_Ch	0.2	20	1.02E6	2.99E5	6.02E3	2.70E4	1.72E4	1.14E3
Incubation	HEL2016_FLA_substrate_14	5	Chondroitin sulfate	2016-03-22T12:00	6	HEL_1_Ch_144	HEL_1_T144_Ch	0.2	20	1.06E6	2.24E5	4.07E4	2.74E4	2.09E4	3.15E3
Incubation	HEL2016_FLA_substrate_15	5	Chondroitin sulfate	2016-03-22T12:00	7	HEL_1_Ch_168		0.2	20	8.39E5	1.50E5	3.26E4	2.78E4	7.44E3	2.08E3
Incubation	HEL2016_FLA_substrate_16	5	Arabinogalactan	2016-03-22T12:00	0	HEL_1_Ar_0	HEL_1_T0_Ar	0.2	20	7.55E5	1.72E5	0.00E0	9.50E3	2.25E3	0.00E0
Incubation	HEL2016_FLA_substrate_17	5	Arabinogalactan	2016-03-22T12:00	1	HEL_1_Ar_24		0.2	20	7.41E5			1.61E4
Incubation	HEL2016_FLA_substrate_18	5	Arabinogalactan	2016-03-22T12:00	3	HEL_1_Ar_72	HEL_1_T72_Ar	0.2	20	1.08E6	4.34E5	7.24E3	1.70E4	3.63E3	9.82E2
Incubation	HEL2016_FLA_substrate_19	5	Arabinogalactan	2016-03-22T12:00	6	HEL_1_Ar_144	HEL_1_T144_Ar	0.2	20	9.74E5	1.16E5	5.14E4	3.40E4	3.70E3	2.65E3
Incubation	HEL2016_FLA_substrate_20	5	Arabinogalactan	2016-03-22T12:00	7	HEL_1_Ar_168		0.2	20	9.20E5	1.37E5	2.74E4	3.50E4	1.28E4	2.47E3
Incubation	HEL2016_FLA_substrate_21	5	Carrageenan	2016-03-22T12:00	0	HEL_1_Ca_0	Hel_1_T0_Ca	0.2	20	8.15E5
Incubation	HEL2016_FLA_substrate_22	5	Carrageenan	2016-03-22T12:00	1	HEL_1_Ca_24		0.2	20	8.53E5
Incubation	HEL2016_FLA_substrate_23	5	Carrageenan	2016-03-22T12:00	3	HEL_1_Ca_72	HEL_1_T72_Ca	0.2	20	1.10E6
Incubation	HEL2016_FLA_substrate_24	5	Carrageenan	2016-03-22T12:00	6	HEL_1_Ca_144		0.2	20	1.81E6
Incubation	HEL2016_FLA_substrate_25	5	Carrageenan	2016-03-22T12:00	7	HEL_1_Ca_168		0.2	20	1.48E6
Incubation	HEL2016_FLA_substrate_26	5	No substrate	2016-03-22T12:00	0	HEL_1_TC_0	Hel_1_T0_N	0.2	20	7.30E5		9.00E2	7.19E3		9.00E2
Incubation	HEL2016_FLA_substrate_27	5	No substrate	2016-03-22T12:00	1	HEL_1_TC_24		0.2	20	7.98E5	2.12E5	1.83E3	1.05E4	2.89E3	1.57E3
Incubation	HEL2016_FLA_substrate_28	5	No substrate	2016-03-22T12:00	3	HEL_1_TC_72	HEL_1_T72_N	0.2	20	8.02E5	2.03E5	1.80E3	8.70E3	6.07E3	4.50E2
Incubation	HEL2016_FLA_substrate_29	5	No substrate	2016-03-22T12:00	6	HEL_1_TC_144	HEL_1_T144_N	0.2	20	1.07E6	2.68E5	4.50E4	1.45E4	9.45E3	4.58E3
Incubation	HEL2016_FLA_substrate_30	5	No substrate	2016-03-22T12:00	7	HEL_1_TC_168		0.2	20	9.48E5	2.14E5	4.33E4	1.47E4	5.15E3	3.30E3
Incubation	HEL2016_FLA_substrate_31	5	Laminarin	2016-04-05T12:00	0	HEL_2_La_0	HEL_2_T0_La	0.2	20	5.84E5	1.33E5	2.95E4	9.00E4	1.66E3	7.94E3
Incubation	HEL2016_FLA_substrate_32	5	Laminarin	2016-04-05T12:00	1	HEL_2_La_24		0.2	20	6.43E5	2.75E5	3.11E2	1.95E5	3.18E4	0.00E0
Incubation	HEL2016_FLA_substrate_33	5	Laminarin	2016-04-05T12:00	3	HEL_2_La_72	HEL_2_T72_La	0.2	20	9.78E5	3.77E5	2.62E4	2.81E5	1.28E5	1.58E3
Incubation	HEL2016_FLA_substrate_34	5	Laminarin	2016-04-05T12:00	6	HEL_2_La_144	Hel_2_T144_La	0.2	20	1.52E6	1.44E5	5.22E5	3.17E5	5.45E4	4.60E4
Incubation	HEL2016_FLA_substrate_35	5	Laminarin	2016-04-05T12:00	9	HEL_2_La_216		0.2	20	4.32E5	2.09E4	9.51E4	8.65E4	5.17E3	1.50E4
Incubation	HEL2016_FLA_substrate_36	5	Xylan	2016-04-05T12:00	0	HEL_2_Xy_0		0.2	20	4.78E5	1.74E5	5.78E2	7.94E4	6.61E4	1.93E2
Incubation	HEL2016_FLA_substrate_37	5	Xylan	2016-04-05T12:00	1	HEL_2_Xy_24		0.2	20	7.11E5	2.62E5	1.98E3	7.22E4	4.66E3	1.98E2
Incubation	HEL2016_FLA_substrate_38	5	Xylan	2016-04-05T12:00	3	HEL_2_Xy_72	HEL_2_T72_Xy	0.2	20	1.92E6	1.26E6	1.02E5	1.19E5	5.47E4	1.07E3
Incubation	HEL2016_FLA_substrate_39	5	Xylan	2016-04-05T12:00	6	HEL_2_Xy_144		0.2	20	1.84E6	3.57E5	4.33E5	1.64E5	4.58E4	7.76E3
Incubation	HEL2016_FLA_substrate_40	5	Xylan	2016-04-05T12:00	9	HEL_2_Xy_216		0.2	20	4.76E5	4.58E4	3.99E4	4.35E4	5.54E3	1.47E3
Incubation	HEL2016_FLA_substrate_41	5	Chondroitin sulfate	2016-04-05T12:00	0	HEL_2_Ch_0	HEL_2_T0_Ch	0.2	20	4.15E5	1.50E5	8.31E2	4.98E4	7.12E4	3.11E2
Incubation	HEL2016_FLA_substrate_42	5	Chondroitin sulfate	2016-04-05T12:00	1	HEL_2_Ch_24		0.2	20	7.94E5	3.40E5	9.22E3	9.78E4	2.83E4	4.50E2
Incubation	HEL2016_FLA_substrate_43	5	Chondroitin sulfate	2016-04-05T12:00	3	HEL_2_Ch_72	HEL_2_T72_Ch	0.2	20	1.27E6	4.69E5	4.26E4	1.65E5	8.83E3	4.37E3
Incubation	HEL2016_FLA_substrate_44	5	Chondroitin sulfate	2016-04-05T12:00	6	HEL_2_Ch_144	Hel_2_T144_Ch	0.2	20	1.39E6	1.62E2	5.88E5	6.83E4	0.00E0	1.09E4
Incubation	HEL2016_FLA_substrate_45	5	Chondroitin sulfate	2016-04-05T12:00	9	HEL_2_Ch_216		0.2	20	2.59E5	6.68E4	6.03E4	1.30E4	2.62E3	2.38E2
Incubation	HEL2016_FLA_substrate_46	5	Arabinogalactan	2016-04-05T12:00	0	HEL_2_Ar_0	HEL_2_T0_Ar	0.2	20	4.40E5	1.58E5	4.34E2	3.53E4	9.60E3	2.89E2
Incubation	HEL2016_FLA_substrate_47	5	Arabinogalactan	2016-04-05T12:00	1	HEL_2_Ar_24		0.2	20	7.50E5	1.65E5	3.40E3	7.60E4	1.17E4	4.86E2
Incubation	HEL2016_FLA_substrate_48	5	Arabinogalactan	2016-04-05T12:00	3	HEL_2_Ar_72	HEL_2_T72_Ar	0.2	20	1.66E6	7.38E5	8.87E4	2.07E5	7.92E4	1.20E4
Incubation	HEL2016_FLA_substrate_49	5	Arabinogalactan	2016-04-05T12:00	6	HEL_2_Ar_144	Hel_2_T144_Ar	0.2	20	8.97E5	1.15E5	3.02E5	9.77E4	1.95E4	2.13E4
Incubation	HEL2016_FLA_substrate_50	5	Arabinogalactan	2016-04-05T12:00	9	HEL_2_Ar_216		0.2	20	2.18E5	1.71E4	2.48E4	1.20E4	2.46E3	1.35E3
Incubation	HEL2016_FLA_substrate_51	5	Carrageenan	2016-04-05T12:00	0	HEL_2_Ca_0	HEL_2_T0_Ca	0.2	20	4.12E5
Incubation	HEL2016_FLA_substrate_52	5	Carrageenan	2016-04-05T12:00	1	HEL_2_Ca_24		0.2	20	1.01E6
Incubation	HEL2016_FLA_substrate_53	5	Carrageenan	2016-04-05T12:00	3	HEL_2_Ca_72	HEL_2_T72_Ca	0.2	20	1.43E6
Incubation	HEL2016_FLA_substrate_54	5	Carrageenan	2016-04-05T12:00	6	HEL_2_Ca_144	HEL_2_T144_Ca	0.2	20	1.91E6
Incubation	HEL2016_FLA_substrate_55	5	Carrageenan	2016-04-05T12:00	9	HEL_2_Ca_216		0.2	20	6.81E5
Incubation	HEL2016_FLA_substrate_56	5	No substrate	2016-04-05T12:00	0	HEL_2_TC_0	HEL_2_T0_N	0.2	20	4.03E5	1.35E5	8.10E2	2.65E4	1.35E4	4.86E2
Incubation	HEL2016_FLA_substrate_57	5	No substrate	2016-04-05T12:00	1	HEL_2_TC_24		0.2	20	6.90E5	3.05E5	2.89E3	5.33E4	8.87E4	1.45E2
Incubation	HEL2016_FLA_substrate_58	5	No substrate	2016-04-05T12:00	3	HEL_2_TC_72	Hel_2_T72_N	0.2	20	1.27E6	6.27E5	3.03E4	1.16E4	5.50E3	3.50E3
Incubation	HEL2016_FLA_substrate_59	5	No substrate	2016-04-05T12:00	6	HEL_2_TC_144	HEL_2_T144_N	0.2	20	7.44E5	1.13E5	1.54E5	2.02E4	5.06E3	4.05E3
Incubation	HEL2016_FLA_substrate_60	5	No substrate	2016-04-05T12:00	9	HEL_2_TC_216		0.2	20	2.11E5	1.33E4	4.03E4	1.80E4	4.22E3	1.35E3
Incubation	HEL2016_FLA_substrate_61	5	Laminarin	2016-04-19T12:00	0	HEL_3_La_0	Hel_3_T0_La	0.2	20	7.38E5	2.52E5	2.02E3	1.28E5	2.95E4	1.84E2
Incubation	HEL2016_FLA_substrate_62	5	Laminarin	2016-04-19T12:00	1	HEL_3_La_24		0.2	20	1.44E6	6.63E5	6.24E3	1.52E5	8.74E4	3.37E2
Incubation	HEL2016_FLA_substrate_63	5	Laminarin	2016-04-19T12:00	3	HEL_3_La_72	Hel_3_T72_La	0.2	20	2.30E6	5.97E5	3.94E5	2.94E5	4.09E4	6.07E3
Incubation	HEL2016_FLA_substrate_64	5	Laminarin	2016-04-19T12:00	6	HEL_3_La_144	Hel_3_T144_La	0.2	20	1.90E6	4.68E5	3.86E5	3.78E5	1.25E5	3.30E4
Incubation	HEL2016_FLA_substrate_65	5	Laminarin	2016-04-19T12:00	9	HEL_3_La_216		0.2	20	8.33E5	9.64E4	2.12E5	1.49E5	3.01E4	3.59E4
Incubation	HEL2016_FLA_substrate_66	5	Xylan	2016-04-19T12:00	0	HEL_3_Xy_0	Hel_3_T0_Xy	0.2	20	7.75E5	2.84E5	1.46E3	2.51E4	6.52E3	4.86E2
Incubation	HEL2016_FLA_substrate_67	5	Xylan	2016-04-19T12:00	1	HEL_3_Xy_24		0.2	20	1.66E6	7.85E5		5.69E4	3.80E4
Incubation	HEL2016_FLA_substrate_68	5	Xylan	2016-04-19T12:00	3	HEL_3_Xy_72	Hel_3_T72_Xy	0.2	20	2.47E6	6.75E5	1.73E5	2.49E5	2.90E4	6.94E3
Incubation	HEL2016_FLA_substrate_69	5	Xylan	2016-04-19T12:00	6	HEL_3_Xy_144	Hel_3_T144_Xy	0.2	20	1.75E6	4.80E5	7.39E4	2.52E5	6.99E4	2.77E3
Incubation	HEL2016_FLA_substrate_70	5	Xylan	2016-04-19T12:00	9	HEL_3_Xy_216		0.2	20	6.75E5	9.12E4	2.66E4	8.99E4	2.18E4	2.34E3
Incubation	HEL2016_FLA_substrate_71	5	Arabinogalactan	2016-04-19T12:00	0	HEL_3_Ar_0	Hel_3_T0_Ar	0.2	20	6.75E5	2.61E5	1.66E3	1.79E4	9.11E3	0.00E0
Incubation	HEL2016_FLA_substrate_72	5	Arabinogalactan	2016-04-19T12:00	1	HEL_3_Ar_24		0.2	20	1.69E6	8.93E5	9.60E3	4.70E4	1.26E4	1.50E2
Incubation	HEL2016_FLA_substrate_73	5	Arabinogalactan	2016-04-19T12:00	3	HEL_3_Ar_72	Hel_3_T72_Ar	0.2	20	2.59E6	1.06E6	2.02E5	2.76E5	1.36E4	2.27E3
Incubation	HEL2016_FLA_substrate_74	5	Arabinogalactan	2016-04-19T12:00	6	HEL_3_Ar_144	Hel_3_T144_Ar	0.2	20	1.32E6	3.99E5	1.08E5	1.99E5	5.81E4	2.89E3
Incubation	HEL2016_FLA_substrate_75	5	Arabinogalactan	2016-04-19T12:00	9	HEL_3_Ar_216		0.2	20	5.87E5	1.02E5	1.98E4	8.30E4	5.03E4	1.30E3
Incubation	HEL2016_FLA_substrate_76	5	Carrageenan	2016-04-19T12:00	0	HEL_3_Ca_0	Hel_3_T0_Ca	0.2	20	5.95E5
Incubation	HEL2016_FLA_substrate_77	5	Carrageenan	2016-04-19T12:00	1	HEL_3_Ca_24		0.2	20	1.67E6
Incubation	HEL2016_FLA_substrate_78	5	Carrageenan	2016-04-19T12:00	3	HEL_3_Ca_72	Hel_3_T72_Ca	0.2	20	3.16E6
Incubation	HEL2016_FLA_substrate_79	5	Carrageenan	2016-04-19T12:00	6	HEL_3_Ca_144	Hel_3_T144_Ca	0.2	20	2.27E6
Incubation	HEL2016_FLA_substrate_80	5	Carrageenan	2016-04-19T12:00	9	HEL_3_Ca_216		0.2	20	1.24E6
Incubation	HEL2016_FLA_substrate_81	5	No substrate	2016-04-19T12:00	0	HEL_3_TC_0	Hel_3_T0_N	0.2	20	7.89E5	2.59E5	2.65E3	1.46E4	4.25E3	4.19E2
Incubation	HEL2016_FLA_substrate_82	5	No substrate	2016-04-19T12:00	1	HEL_3_TC_24		0.2	20	1.99E6	8.64E5	1.51E4	1.20E4	7.11E3	2.70E2
Incubation	HEL2016_FLA_substrate_83	5	No substrate	2016-04-19T12:00	3	HEL_3_TC_72	Hel_3_T72_N	0.2	20	2.69E6	1.06E6	1.48E5	2.05E4	1.37E4	4.50E2
Incubation	HEL2016_FLA_substrate_84	5	No substrate	2016-04-19T12:00	6	HEL_3_TC_144		0.2	20	1.49E6	6.20E5	1.18E5	1.09E4	4.61E3	2.53E2
Incubation	HEL2016_FLA_substrate_85	5	No substrate	2016-04-19T12:00	9	HEL_3_TC_216		0.2	20	7.21E5	1.34E5	9.09E4	4.31E4	9.16E3	1.05E3
Incubation	HEL2016_FLA_substrate_86	5	Laminarin	2016-05-03T12:00	0	HEL_4_La_0	Hel_4_T0_La	0.2	20	1.19E6	3.65E5	1.56E2	3.04E5	1.00E5	1.56E2
Incubation	HEL2016_FLA_substrate_87	5	Laminarin	2016-05-03T12:00	1	HEL_4_La_24		0.2	20	1.97E6	7.48E5	1.70E3	4.65E5	1.35E5	1.31E2
Incubation	HEL2016_FLA_substrate_88	5	Laminarin	2016-05-03T12:00	3	HEL_4_La_72	Hel_4_T72_La	0.2	20	1.99E6	5.31E5	3.44E5	4.64E5	1.74E5	2.22E4
Incubation	HEL2016_FLA_substrate_89	5	Laminarin	2016-05-03T12:00	6	HEL_4_La_144	Hel_4_T144_La	0.2	20	9.10E5	1.08E5	6.85E4	1.29E5	2.64E4	8.88E3
Incubation	HEL2016_FLA_substrate_90	5	Laminarin	2016-05-03T12:00	9	HEL_4_La_216		0.2	20	6.37E5	7.63E4	1.01E4	5.55E4	2.23E4	2.30E3
Incubation	HEL2016_FLA_substrate_91	5	Xylan	2016-05-03T12:00	0	HEL_4_Xy_0	Hel_4_T0_Xy	0.2	20	1.19E6	2.96E5	4.76E2	4.68E4	6.07E3	2.38E2
Incubation	HEL2016_FLA_substrate_92	5	Xylan	2016-05-03T12:00	1	HEL_4_Xy_24		0.2	20	2.26E6	9.95E5	4.86E3	2.05E5	7.56E4	1.62E2
Incubation	HEL2016_FLA_substrate_93	5	Xylan	2016-05-03T12:00	3	HEL_4_Xy_72	Hel_4_T72_Xy	0.2	20	1.51E6	4.00E5	1.74E5	2.40E5	7.35E4	1.37E4
Incubation	HEL2016_FLA_substrate_94	5	Xylan	2016-05-03T12:00	6	HEL_4_Xy_144	Hel_4_T144_Xy	0.2	20	9.44E5	1.20E5	2.52E4	1.08E5	4.50E3	2.34E3
Incubation	HEL2016_FLA_substrate_95	5	Xylan	2016-05-03T12:00	9	HEL_4_Xy_216		0.2	20	6.05E5	6.16E4	2.34E3	1.93E4	1.64E3	3.20E2
Incubation	HEL2016_FLA_substrate_96	5	Chondroitin sulfate	2016-05-03T12:00	0	HEL_4_Ch_0	Hel_4_T0_Ch	0.2	20	1.30E6	3.53E5	4.91E2	1.41E4	4.53E3	3.68E2
Incubation	HEL2016_FLA_substrate_97	5	Chondroitin sulfate	2016-05-03T12:00	1	HEL_4_Ch_24		0.2	20	2.28E6	9.64E5	2.87E3	3.10E4	1.03E4	0.00E0
Incubation	HEL2016_FLA_substrate_98	5	Chondroitin sulfate	2016-05-03T12:00	3	HEL_4_Ch_72	Hel_4_T72_Ch	0.2	20	1.47E6	5.02E5	2.43E3	1.44E5	1.19E4	2.70E2
Incubation	HEL2016_FLA_substrate_99	5	Chondroitin sulfate	2016-05-03T12:00	6	HEL_4_Ch_144	Hel_4_T144_Ch	0.2	20	8.65E5	1.79E5	3.75E4	2.03E4	8.73E3	6.39E2
Incubation	HEL2016_FLA_substrate_100	5	Chondroitin sulfate	2016-05-03T12:00	9	HEL_4_Ch_216		0.2	20	7.33E5	2.55E5	1.63E4	1.47E4	2.13E3	8.38E2
Incubation	HEL2016_FLA_substrate_101	5	Arabinogalactan	2016-05-03T12:00	0	HEL_4_Ar_0	Hel_4_T0_Ar	0.2	20	1.26E6	3.85E5	1.56E2	1.58E4	7.29E3	0.00E0
Incubation	HEL2016_FLA_substrate_102	5	Arabinogalactan	2016-05-03T12:00	1	HEL_4_Ar_24		0.2	20	2.36E6	1.02E6	2.65E3	3.57E4	1.51E4	6.23E2
Incubation	HEL2016_FLA_substrate_103	5	Arabinogalactan	2016-05-03T12:00	3	HEL_4_Ar_72	Hel_4_T72_Ar	0.2	20	1.97E6	5.59E5	1.12E5	2.14E5	4.99E4	3.00E3
Incubation	HEL2016_FLA_substrate_104	5	Arabinogalactan	2016-05-03T12:00	6	HEL_4_Ar_144	Hel_4_T144_Ar	0.2	20	7.52E5	1.47E5	2.11E4	2.59E4	5.74E3	2.75E3
Incubation	HEL2016_FLA_substrate_105	5	Arabinogalactan	2016-05-03T12:00	9	HEL_4_Ar_216		0.2	20	8.80E5	1.63E5	6.46E3	6.82E4	3.61E4	7.66E2
Incubation	HEL2016_FLA_substrate_106	5	Carrageenan	2016-05-03T12:00	0	HEL_4_Ca_0	Hel_4_T0_Ca	0.2	20	1.21E6
Incubation	HEL2016_FLA_substrate_107	5	Carrageenan	2016-05-03T12:00	1	HEL_4_Ca_24		0.2	20	1.56E6
Incubation	HEL2016_FLA_substrate_108	5	Carrageenan	2016-05-03T12:00	3	HEL_4_Ca_72	Hel_4_T72_Ca	0.2	20	1.40E6
Incubation	HEL2016_FLA_substrate_109	5	Carrageenan	2016-05-03T12:00	6	HEL_4_Ca_144	Hel_4_T144_Ca	0.2	20	1.15E6
Incubation	HEL2016_FLA_substrate_110	5	Carrageenan	2016-05-03T12:00	9	HEL_4_Ca_216		0.2	20	1.17E6
Incubation	HEL2016_FLA_substrate_111	5	No substrate	2016-05-03T12:00	0	HEL_4_TC_0	Hel_4_T0_N	0.2	20	1.39E6	3.98E5	7.46E2	1.39E4	7.09E3	2.13E2
Incubation	HEL2016_FLA_substrate_112	5	No substrate	2016-05-03T12:00	1	HEL_4_TC_24		0.2	20	2.31E6	9.27E5	1.12E3	2.07E4	1.21E4	0.00E0
Incubation	HEL2016_FLA_substrate_113	5	No substrate	2016-05-03T12:00	3	HEL_4_TC_72	Hel_4_T72_N	0.2	20	1.70E6	6.07E5	4.60E3	1.32E5	8.50E4	1.09E2
Incubation	HEL2016_FLA_substrate_114	5	No substrate	2016-05-03T12:00	6	HEL_4_TC_144	Hel_4_T144_N	0.2	20	6.35E5	1.02E5	1.99E4	1.86E4	3.71E3	1.77E3
Incubation	HEL2016_FLA_substrate_115	5	No substrate	2016-05-03T12:00	9	HEL_4_TC_216		0.2	20	8.49E5	8.03E4	7.20E3	1.52E4	5.57E3	1.57E3