Citation:

PANGAEA.903579

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Abstract:

Spring phytoplankton blooms contribute significantly to global marine primary production. A large fraction of the bloom derived organic matter is available to heterotrophic bacteria in the form of polysaccharides. We analyzed changes in the modes of polysaccharide utilization (selfish uptake and extracellular hydrolysis) during a spring phytoplankton bloom using fluorescently labelled polysaccharide incubations coupled with 16s rRNA sequencing and fluorescence in situ hybridization. We found that in the early bloom phases there was high selfish activity of simple polysaccharides (laminarin) and low extracellular hydrolysis rates of a limited range of polysaccharides. During the course of the bloom both the selfish uptake and extracellular hydrolysis rates increased but only for a limited range of substrates. At the late bloom phase a wide range of substrate was extracellularly hydrolyzed and the level of selfish uptake decreased. We found that during a spring phytoplankton bloom the mode of substrate utilization depended on both the substrates structural complexity and the composition of the heterotrophic community related to the bloom phase.

Supplement to:

Reintjes, Greta; Fuchs, Bernhard M; Scharfe, Mirco; Wiltshire, Karen Helen; Amann, Rudolf; Arnosti, Carol (2020): Short‐term changes in polysaccharide utilization mechanisms of marine bacterioplankton during a spring phytoplankton bloom. Environmental Microbiology, 22(5), 1884-1900, https://doi.org/10.1111/1462-2920.14971

Coverage:

Latitude: 54.188330 * Longitude: 7.900000

Date/Time Start: 2016-03-22T12:00:00 * Date/Time End: 2016-05-03T12:00:00

Minimum DEPTH, water: 5 m * Maximum DEPTH, water: 5 m

Event(s):

HelgolandRoads_site (Kabeltonne) * Latitude: 54.188330 * Longitude: 7.900000 * Elevation: -10.0 m * DEIMS ID: deims.org * Location: German Bight, North Sea * Comment: Samples and Measurements were taken at HelgolandRoads site but independently of Helgoland Roads LTER project

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Device type	Device		Reintjes, Greta
2	Sample code/label	Sample label		Reintjes, Greta
3	DEPTH, water	Depth water	m	Reintjes, Greta		Geocode
4	Substrate type	Substrate		Reintjes, Greta
5	DATE/TIME	Date/Time		Reintjes, Greta		Geocode
6	Experiment day	Exp day	day	Reintjes, Greta
7	Sample ID	Sample ID		Reintjes, Greta
8	Sample ID	Sample ID		Reintjes, Greta		ENA
9	Size	Size	µm	Reintjes, Greta
10	Volume	Vol	ml	Reintjes, Greta
11	Bacterioplankton	Bacterioplankton	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
12	Bacteroidetes, targeted with CF319a oligonucliotide FISH-Probe	CF319a	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
13	Alteromonadales, targeted with ALT1413 oligonucleiotide FISH-Probe	ALT1413	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
14	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)	of FLA-substrate stained cells
15	Bacteroidetes, substrate stained	Bacteroidetes sub stain	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
16	Alteromonadales, substrate stained	Alteromonadales sub stain	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Size:

1448 data points

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