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Bechmann, Renée Katrin; Lyng, Emily; Westerlund, Stig; Bamber, Shaw D; Berry, Mark; Arnberg, Maj; Kringstad, Alfhild; Calosi, Piero; Seear, Paul J (2018): Seawater carbonate chemistry and survival, swimming activity, feeding rate of Northern shrimp (Pandalus borealis) [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.903546, Supplement to: Bechmann, RK et al. (2018): Early life stages of Northern shrimp (Pandalus borealis) are sensitive to fish feed containing the anti-parasitic drug diflubenzuron. Aquatic Toxicology, 198, 82-91, https://doi.org/10.1016/j.aquatox.2018.02.021

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Abstract:
Increasing use of fish feed containing the chitin synthesis inhibiting anti-parasitic drug diflubenzuron (DFB) in salmon aquaculture has raised concerns over its impact on coastal ecosystems. Larvae of Northern shrimp (Pandalus borealis) were exposed to DFB medicated feed under Control conditions (7.0 °C, pH 8.0) and under Ocean Acidification and Warming conditions (OAW, 9.5 °C and pH 7.6). Two weeks' exposure to DFB medicated feed caused significantly increased mortality. The effect of OAW and DFB on mortality of shrimp larvae was additive; 10% mortality in Control, 35% in OAW, 66% in DFB and 92% in OAW + DFB. In OAW + DFB feeding and swimming activity were reduced for stage II larvae and none of the surviving larvae developed to stage IV. Two genes involved in feeding (GAPDH and PRLP) and one gene involved in moulting (DD9B) were significantly downregulated in larvae exposed to OAW + DFB relative to the Control. Due to a shorter intermoult period under OAW conditions, the OAW + DFB larvae were exposed throughout two instead of one critical pre-moult period. This may explain the more serious sub-lethal effects for OAW + DFB than DFB larvae. A single day exposure at 4 days after hatching did not affect DFB larvae, but high mortality was observed for OAW + DFB larvae, possibly because they were exposed closer to moulting. High mortality of shrimp larvae exposed to DFB medicated feed, indicates that the use of DFB in salmon aquaculture is a threat to crustacean zooplankton.
Keyword(s):
Animalia; Arthropoda; Behaviour; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Gene expression (incl. proteomics); Laboratory experiment; Mortality/Survival; North Atlantic; Other; Pandalus borealis; Pelagos; Single species; Temperate; Temperature; Zooplankton
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb
Coverage:
Latitude: 59.066670 * Longitude: 5.750000
Date/Time Start: 2014-02-10T00:00:00 * Date/Time End: 2015-03-13T00:00:00
Event(s):
Hillefjord * Latitude: 59.066670 * Longitude: 5.750000 * Date/Time Start: 2014-02-10T00:00:00 * Date/Time End: 2015-03-13T00:00:00 * Method/Device: Experiment (EXP)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-07-01.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
TypeTypeBechmann, Renée Katrinstudy
SpeciesSpeciesBechmann, Renée Katrin
Registration number of speciesReg spec noBechmann, Renée Katrin
Uniform resource locator/link to referenceURL refBechmann, Renée KatrinWoRMS Aphia ID
ExperimentExpBechmann, Renée Katrin
TreatmentTreatBechmann, Renée Katrin
TreatmentTreatBechmann, Renée KatrinDFB exposure
Time in daysTimedaysBechmann, Renée Katrinage of larvae
PercentagePerc%Bechmann, Renée Katrin% survivors
10 Percentage, standard deviationPerc std dev±Bechmann, Renée Katrin% survivors
11 PercentagePerc%Bechmann, Renée Katrin% stage I larvae of the survivors
12 Percentage, standard deviationPerc std dev±Bechmann, Renée Katrin% stage I larvae of the survivors
13 PercentagePerc%Bechmann, Renée Katrin% stage II larvae of the survivors
14 Percentage, standard deviationPerc std dev±Bechmann, Renée Katrin% stage II larvae of the survivors
15 PercentagePerc%Bechmann, Renée Katrin% stage III larvae of the survivors
16 Percentage, standard deviationPerc std dev±Bechmann, Renée Katrin% stage III larvae of the survivors
17 PercentagePerc%Bechmann, Renée Katrin% stage IV larvae of the survivors
18 Percentage, standard deviationPerc std dev±Bechmann, Renée Katrin% stage IV larvae of the survivors
19 Numbern#Bechmann, Renée Katrinaquaria
20 StageStageBechmann, Renée Katrin
21 Swimming activity, beam breaks per hourSwim act#/hBechmann, Renée Katrin
22 Swimming activity, standard deviationSwim act std dev±Bechmann, Renée Katrin
23 IndividualsInd#Bechmann, Renée KatrinArtemia eaten
24 Feeding rate per individualFeed rate/ind#/#/hBechmann, Renée KatrinArtemia eaten pr shrimp larvae pr hour
25 Gene nameGeneBechmann, Renée Katrin
26 Gene expression, fold change, relativeGene expressionBechmann, Renée Katrin
27 Gene expression, fold change, relative, standard errorGene expression std e±Bechmann, Renée Katrin
28 SalinitySalBechmann, Renée Katrin
29 Temperature, waterTemp°CBechmann, Renée Katrin
30 pHpHBechmann, Renée KatrinNBS scale
31 Alkalinity, totalATµmol/kgBechmann, Renée Katrin
32 Carbon, inorganic, dissolvedDICµmol/kgBechmann, Renée Katrin
33 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmBechmann, Renée Katrin
34 Calcite saturation stateOmega CalBechmann, Renée Katrin
35 Aragonite saturation stateOmega ArgBechmann, Renée Katrin
36 Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
37 pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
38 Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
39 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
40 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
41 Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
42 Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
43 Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
44 Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
45 Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
3888 data points

Data

Download dataset as tab-delimited text — use the following character encoding:


Type
(study)

Species

Reg spec no

URL ref
(WoRMS Aphia ID)

Exp

Treat

Treat
(DFB exposure)

Time [days]
(age of larvae)

Perc [%]
(% survivors)
10 
Perc std dev [±]
(% survivors)
11 
Perc [%]
(% stage I larvae of the survi...)
12 
Perc std dev [±]
(% stage I larvae of the survi...)
13 
Perc [%]
(% stage II larvae of the surv...)
14 
Perc std dev [±]
(% stage II larvae of the surv...)
15 
Perc [%]
(% stage III larvae of the sur...)
16 
Perc std dev [±]
(% stage III larvae of the sur...)
17 
Perc [%]
(% stage IV larvae of the surv...)
18 
Perc std dev [±]
(% stage IV larvae of the surv...)
19 
n [#]
(aquaria)
20 
Stage
21 
Swim act [#/h]
22 
Swim act std dev [±]
23 
Ind [#]
(Artemia eaten)
24 
Feed rate/ind [#/#/h]
(Artemia eaten pr shrimp larva...)
25 
Gene
26 
Gene expression
27 
Gene expression std e [±]
28 
Sal
29 
Temp [°C]
30 
pH
(NBS scale)
31 
AT [µmol/kg]
32 
DIC [µmol/kg]
33 
pCO2water_SST_wet [µatm]
34 
Omega Cal
35 
Omega Arg
36 
CSC flag
37 
pH
(total scale)
38 
CO2 [µmol/kg]
39 
fCO2water_SST_wet [µatm]
40 
pCO2water_SST_wet [µatm]
41 
[HCO3]- [µmol/kg]
42 
[CO3]2- [µmol/kg]
43 
DIC [µmol/kg]
44 
Omega Arg
45 
Omega Cal
laboratoryPandalus borealis (crustacean)107649marinespecies.org1ControlNo exposure69721000000000532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFBDFB exposure 1-14 dph. Depuration 15-27 dph.69391000000000632.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAWNo exposure6869192081200000632.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW+DFBDFB exposure day 1-14. Depuration day 15-19.66221602640260000632.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1ControlNo exposure9963101890180000532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFBDFB exposure 1-14 dph. Depuration 15-27 dph.98811141686160000632.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAWNo exposure981100010000000632.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW+DFBDFB exposure 1-14 dph. Depuration 15-19 dph.939190010000000632.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1ControlNo exposure139340010000000532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFBDFB exposure 1-14 dph. Depuration 15-27 dph.1361240010000000632.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAWNo exposure137115001715831500632.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW+DFBDFB exposure 1-14 dph. Depuration 15-19 dph.132019009921200632.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1ControlNo exposure17915004424562400532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFBDFB exposure 1-14 dph. Depuration 15-27 dph.173923009743400632.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAWNo exposure176818005493726632.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW+DFBDFB exposure 1-14 dph. Depuration 15-19 dph.179160065502500632.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1ControlNo exposure19906001412861200532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFBDFB exposure 1-14 dph. Depuration 15-27 dph.193422003621642100632.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAWNo exposure196518002627187022632.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW+DFBDFB exposure 1-14 dph. Depuration 15-19 dph.19815003943111500632.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1ControlNo exposure278311009069067513532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFBDFB exposure 1-14 dph. Depuration 15-27 dph.27282100101343264821632.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org2ControlNo exposure11982432.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org2DFBDFB exposure for two days only: 4 dph and 16 dph11964432.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org2OAWNo exposure117925432.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org2OAW+DFBDFB exposure for one day only: 4 dph112626432.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org2ControlNo exposure22918432.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org2DFBDFB exposure for two days only: 4 dph and 16 dph22934432.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org2OAWNo exposure226627332.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org2OAW+DFBDFB exposure for one day only: 4 dph221914432.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org2ControlNo exposure338112432.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org2DFBDFB exposure for two days only: 4 dph and 16 dph33895432.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org2OAWNo exposure336420432.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org2OAW+DFBDFB exposure for one day only: 4 dph331812432.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control1137035832.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB1215257732.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW11804101032.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW+DFB1102068432.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control267336532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB272166532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW2995101832.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW+DFB28616732.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control474253432.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB469263632.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW454970432.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1OAW+DFB4032.89.507.582315.92334.01697.20.930.59267.4775.931680.511687.062222.5737.672336.170.580.91
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control2662.632.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control2542.232.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control2732.932.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control2883.532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control2411.632.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control2251.032.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control2130.532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control2190.832.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control2632.532.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1Control2692.832.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB2582.332.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB2542.732.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB2281.132.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB2351.432.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB2301.232.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB2160.632.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB2190.832.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB2341.432.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
laboratoryPandalus borealis (crustacean)107649marinespecies.org1DFB2512.032.86.958.002315.92207.9648.02.191.40267.9028.92586.76589.122103.0985.632217.641.302.07
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