Citation:

EN17130

Name: Seawater carbonate chemistry and trace gas emissions in two microalgae Phaeocystis globosa and Nitzschia closterium
Published: 2017
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Haptophyta; Laboratory experiment; Laboratory strains; Nitzschia closterium; Not applicable; Ochrophyta; Other metabolic rates; Pelagos; Phaeocystis globosa; Phytoplankton; Single species

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Abstract:

The potential impacts of seawater acidification on the concentrations of dimethylsulfide (DMS), dimethylsulfoniopropionate (DMSP), dissolved acrylic acid (AAd) and various volatile halocarbons, including CH3Cl, CHBr3, CH2Br2, CHBr2Cl, CHBrCl2 and CH3I, were examined during a laboratory CO2 perturbation experiment for the microalgae Phaeocystis globosa and Nitzschia closterium. The microalgae were exposed to ambient CO2 conditions (390–540 µatm; 1 µatm = 0.1 Pa) and to projected concentrations for the end of the century (760–1000 µatm, high carbon (HC)). The growth rate of the two species remained unaffected by elevated CO2. Results showed a 48 and 37 % decline in the DMS concentration normalised to cell density in P. globosa and N. closterium cultures in the HC treatment compared with the ambient treatment. No significant difference was observed for DMSPp and DMSPd in the two microalgae cultures between the two CO2 levels. The mean AAd concentrations in the P. globosa culture showed a 28 % decline in the HC treatment. By contrast, the cell-normalised concentrations of AAd in the HC treatment were 45 % lower than in the ambient treatment in N. closterium cultures. No CO2-induced effects were observed for CH3Cl, CHBr3, CHBr2Cl, CHBrCl2 and CH3I, but cell-normalised concentrations of CH2Br2 in N. closterium cultures showed a 32 % decline in the HC treatment relative to the ambient level. These results show that the metabolism processes responsible for the production of climate-active gases in phytoplankton may be affected by high CO2 levels. There may be a potential delay in the responses of trace gas emissions to elevated pCO2.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Haptophyta; Laboratory experiment; Laboratory strains; Nitzschia closterium; Not applicable; Ochrophyta; Other metabolic rates; Pelagos; Phaeocystis globosa; Phytoplankton; Single species

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-07-01.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Yang, Guipeng		study
2	Species	Species		Yang, Guipeng
3	Registration number of species	Reg spec no		Yang, Guipeng
4	Uniform resource locator/link to reference	URL ref		Yang, Guipeng		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Yang, Guipeng
6	Treatment	Treat		Yang, Guipeng
7	Cell density	Cells	#/ml	Yang, Guipeng
8	Chlorophyll a	Chl a	µg/l	Yang, Guipeng
9	Dimethyl sulfide	DMS	nmol/l	Yang, Guipeng
10	Indium	In	mg/kg	Yang, Guipeng
11	Dimethylsulfoniopropionate, particulate	DMSP part	nmol/l	Yang, Guipeng
12	Chloromethane	CH3Cl	pmol/l	Yang, Guipeng
13	Iodomethane	CH3I	pmol/l	Yang, Guipeng
14	Dibromochloromethane	CHBr2Cl	pmol/l	Yang, Guipeng
15	Tribromomethane	CHBr3	pmol/l	Yang, Guipeng
16	Bromodichloromethane	CHBrCl2	pmol/l	Yang, Guipeng
17	Dibromomethane	CH2Br2	pmol/l	Yang, Guipeng
18	Acrylic acid, dissolved	AAd	nmol/l	Yang, Guipeng
19	pH	pH		Yang, Guipeng		NBS scale
20	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Guipeng
21	Salinity	Sal		Yang, Guipeng
22	Temperature, water	Temp	°C	Yang, Guipeng
23	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
24	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
25	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
26	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
27	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
29	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1554 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Treat	7 Cells [#/ml]	8 Chl a [µg/l]	9 DMS [nmol/l]	10 In [mg/kg]	11 DMSP part [nmol/l]	12 CH3Cl [pmol/l]	13 CH3I [pmol/l]	14 CHBr2Cl [pmol/l]	15 CHBr3 [pmol/l]	16 CHBrCl2 [pmol/l]	17 CH2Br2 [pmol/l]	18 AAd [nmol/l]	19 pH (NBS scale)	20 DIC [µmol/kg]	21 Sal	22 Temp [°C]	23 CSC flag	24 pH (total scale)	25 CO2 [µmol/kg]	26 fCO2water_SST_wet [µatm]	27 pCO2water_SST_wet [µatm]	28 [HCO3]- [µmol/kg]	29 [CO3]2- [µmol/kg]	30 AT [µmol/kg]	31 Omega Arg	32 Omega Cal
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	6	High CO2	1922	0.25	7.34	6.94	9.80	55.26	4.28	7.70	49.62	2.14	5.47	48.24	7.78	1922	32	25	27	7.64	29.95	1038.42	1041.74	1812.27	79.79	2010.72	1.29	1.97
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	7	High CO2	1907	0.25	6.99	6.12	7.30	107.42	9.07	7.20	111.83	3.99	6.23	33.18	7.79	1907	32	25	27	7.65	29.02	1006.27	1009.49	1797.02	80.96	1998.64	1.31	2.00
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	8	High CO2	1948	0.25	7.42	5.12	9.42	66.49	5.06	4.54	89.26	1.86	5.53	22.43	7.69	1948	32	25	27	7.55	37.50	1300.32	1304.49	1844.50	66.00	2008.63	1.07	1.63
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	9	High CO2	1943	0.50	7.15	3.75	7.02	97.30	5.91	2.80	42.25	7.57	5.90	29.34	7.75	1943	32	25	27	7.61	32.49	1126.75	1130.36	1835.11	75.40	2022.40	1.22	1.86
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	10	High CO2	2005	0.50	7.47	4.90	11.84	65.56	8.97	2.78	42.19	11.97	6.23	22.15	7.76	2005	32	25	27	7.62	32.75	1135.63	1139.27	1892.68	79.57	2089.10	1.29	1.96
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	11	High CO2	2130	2.00	6.34	6.60	11.91	43.00	7.27	3.64	44.94	3.08	6.21		7.78	2130	32	25	27	7.64	33.19	1150.82	1154.51	2008.39	88.42	2224.11	1.43	2.18
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	12	High CO2	1934	2.75	7.81	4.75	8.40	38.51	13.15	5.18	79.36	10.07	6.58		7.77	1934	32	25	27	7.63	30.85	1069.86	1073.29	1824.64	78.50	2019.72	1.27	1.94
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	13	High CO2	2118	2.00	10.28	4.91	11.91	48.32	4.32	4.54	42.07	1.52	6.28		7.78	2118	32	25	27	7.64	33.00	1144.33	1148.00	1997.08	87.92	2211.80	1.42	2.17
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	14	High CO2	2115	2.50	8.02			38.88	8.28	5.73	85.30	2.66	6.93	21.65	7.84	2115	32	25	27	7.70	28.58	991.09	994.27	1986.02	100.40	2230.88	1.62	2.48
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	15	High CO2	2113	2.00	6.48	5.58	12.46	44.92	5.39	4.45	58.93	2.93	5.69	21.57	7.82	2113	32	25	27	7.68	29.95	1038.43	1041.76	1987.13	95.93	2221.25	1.55	2.37
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	16	High CO2	1992	2.00	9.56	9.30	5.18	67.05	3.10	5.26	111.76	8.07	6.03	24.55	7.79	1992	32	25	27	7.65	30.31	1051.09	1054.45	1877.12	84.57	2085.96	1.37	2.09
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	17	High CO2	1879	1.75	6.98	7.63	14.48	37.03	6.28		59.84	5.59	4.68	52.08	7.84	1879	32	25	27	7.70	25.39	880.54	883.36	1764.42	89.19	1986.86	1.44	2.20
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	18	High CO2	2005	2.50	13.10	7.19	19.56							32.70	7.83	2005	32	25	27	7.69	27.75	962.18	965.27	1884.17	93.08	2113.49	1.50	2.30
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	19	High CO2	2155	2.75	15.73	4.70	11.35							30.87	7.90	2155	32	25	27	7.76	25.23	874.88	877.68	2012.93	116.84	2296.46	1.89	2.88
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	6	Ambient	2048	0.25	11.50	9.50	8.16	57.99	4.47	8.25	57.86	2.02	6.70	41.94	8.11	2048	32	25	27	7.97	14.38	498.73	500.33	1858.84	174.78	2283.85	2.82	4.31
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	7	Ambient	2028	0.25	13.67	6.00	7.06	73.97	10.61	7.26	107.42	5.08	6.35	26.91	8.10	2028	32	25	27	7.96	14.56	504.82	506.44	1843.60	169.85	2257.48	2.74	4.19
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	8	Ambient	2054	0.25	13.27	5.30	10.32	84.22	6.57	4.12	66.49	3.08	4.91	34.63	8.02	2054	32	25	27	7.88	17.96	622.87	624.86	1891.18	144.86	2244.38	2.34	3.57
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	9	Ambient	2234	0.50	15.89	4.43	9.91	37.71	13.57	2.91	60.30	8.79	7.28	38.17	8.01	2234	32	25	27	7.87	20.06	695.60	697.83	2060.04	153.90	2429.99	2.49	3.80
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	10	Ambient	2051	0.75	17.61	4.53	8.87	103.31	9.84	2.79	38.91	7.98	6.45	35.33	8.10	2051	32	25	27	7.96	14.75	511.43	513.07	1864.73	171.52	2281.86	2.77	4.23
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	11	Ambient	2238	3.25	17.46	6.92	11.12	42.27	7.31	3.67	36.47	3.07	6.54		8.08	2238	32	25	27	7.94	16.92	586.66	588.54	2041.81	179.27	2471.62	2.90	4.42
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	12	Ambient	2028	3.25	22.08	6.01	9.65	41.73	17.08	8.01	86.43	11.48	8.18		8.06	2028	32	25	27	7.92	16.07	557.20	558.99	1855.98	155.95	2236.57	2.52	3.85
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	13	Ambient	2231	2.25	14.00	4.09	14.39	38.13	5.41	5.19	36.58	3.03	7.28		8.06	2231	32	25	27	7.92	17.67	612.82	614.78	2041.72	171.60	2453.63	2.77	4.23
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	14	Ambient	2182	2.25	19.03			38.20	4.32	5.29	75.88	2.96	7.36	30.79	8.07	2182	32	25	27	7.93	16.89	585.78	587.65	1993.90	171.21	2406.27	2.77	4.22
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	15	Ambient	2020	1.75	20.27	5.47	8.63	40.85	6.20	4.92	76.39	8.32	6.61	44.43	8.08	2020	32	25	27	7.94	15.24	528.58	530.27	1842.70	162.06	2238.18	2.62	4.00
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	16	Ambient	2031	1.25	23.74	5.46	11.24	82.06	3.03	5.72	107.05	8.17	7.66	67.76	8.11	2031	32	25	27	7.97	14.26	494.57	496.15	1843.41	173.33	2265.53	2.80	4.28
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	17	Ambient	2011	2.00	22.21	5.46	17.40	41.00	14.64		59.68	13.03	8.13	65.14	8.13	2011	32	25	27	7.99	13.45	466.44	467.94	1818.67	178.88	2254.75	2.89	4.41
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	18	Ambient	2215	3.00	31.39	6.89	14.99							55.60	8.09	2215	32	25	27	7.95	16.31	565.52	567.34	2017.18	181.51	2452.95	2.93	4.48
laboratory	Phaeocystis globosa (phytoplankton)	160538	marinespecies.org	19	Ambient	2210	3.00	23.59	10.22	11.39							30.42	8.16	2210	32	25	27	8.02	13.71	475.38	476.90	1986.82	209.48	2488.66	3.38	5.17
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	6	High CO2	10000	0.63	6.04	4.81	5.17	58.44	4.45	6.93	94.11	9.75	5.23	26.91	7.71	2346	32	25	27	7.57	43.02	1491.64	1496.43	2219.66	83.32	2419.89	1.35	2.06
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	7	High CO2	12500	1.26	4.70	5.38	4.73	63.27	7.65	6.90	51.71	3.86	4.83	31.84	7.69	2330	32	25	27	7.55	44.82	1554.28	1559.26	2206.18	79.00	2396.32	1.28	1.95
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	8	High CO2	20000	2.30	6.51	4.71	5.92	66.67	3.92	4.56	93.22	4.00	4.68	33.14	7.56	2354	32	25	27	7.42	61.10	2118.70	2125.50	2233.50	59.40	2376.61	0.96	1.47
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	9	High CO2	40000	3.21	8.10	4.38	5.33	38.93	4.56	2.84	54.88	8.65	4.54	34.00	7.61	2341	32	25	27	7.47	54.12	1876.68	1882.70	2220.60	66.28	2380.28	1.07	1.63
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	10	High CO2	50000	4.65	6.52	4.12	5.16	49.02	9.57	2.81	56.25	12.06	4.78	17.73	7.69	2350	32	25	27	7.55	45.38	1573.75	1578.80	2225.24	79.37	2416.02	1.28	1.96
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	11	High CO2	57500	7.96	6.38	9.92	5.07	49.15	11.67	3.69	60.11	5.85	3.97		7.73	2155	32	25	27	7.59	37.90	1314.11	1318.33	2037.42	79.69	2231.67	1.29	1.97
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	12	High CO2	70000	12.80	6.77	4.63	9.33	43.10	11.24	6.58	77.06	14.75	4.94		7.70	2338	32	25	27	7.56	43.91	1522.66	1527.54	2212.95	81.13	2408.08	1.31	2.00
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	13	High CO2	105000	21.71	11.49	8.08	7.47	40.17	5.82	4.32	68.58	2.73	4.59		7.74	2345	32	25	27	7.60	40.03	1388.02	1392.47	2215.74	89.23	2430.03	1.44	2.20
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	14	High CO2	112500	25.90	7.87			44.24	6.44	5.57	84.29	2.73	4.76	23.13	7.84	2307	32	25	27	7.70	31.25	1083.45	1086.92	2166.47	109.28	2429.02	1.77	2.70
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	15	High CO2	230000	46.68	7.60	8.23	5.99	47.54	6.49	4.89	76.60	2.77	5.22	25.25	7.86	2320	32	25	27	7.72	29.82	1034.21	1037.52	2174.80	115.37	2451.56	1.86	2.85
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	16	High CO2	212500	58.45	10.50	4.34	5.80	68.63	2.04	4.34	95.43	5.32	5.72	33.44	8.04	2261	32	25	27	7.90	18.82	652.55	654.64	2075.63	166.55	2474.79	2.69	4.11
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	6	Ambient	7500	0.92	6.16	6.22	4.53	40.43	4.43	10.95	78.37	13.17	4.75	36.68	7.91	2244	32	25	27	7.77	25.60	887.70	890.55	2093.81	124.59	2393.95	2.01	3.07
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	7	Ambient	10000	1.14	5.84	3.92	5.37	54.64	4.43	6.96	94.44	9.78	5.24	31.53	7.91	2253	32	25	27	7.77	25.72	891.78	894.64	2102.26	125.02	2403.24	2.02	3.08
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	8	Ambient	12500	2.59	7.44	4.92	4.94	74.92	4.55	4.45	101.13	2.13	5.18	36.47	7.84	2281	32	25	27	7.70	30.87	1070.55	1073.98	2142.01	108.12	2402.25	1.75	2.67
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	9	Ambient	15000	2.29	6.27	3.28	4.72	39.21	7.41	3.28	40.02	11.09	4.35	40.38	7.86	2254	32	25	27	7.72	29.03	1006.80	1010.03	2113.09	111.88	2382.78	1.81	2.76
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	10	Ambient	20000	5.50	6.83	4.05	3.32	51.56	8.56	2.80	49.09	11.97	5.15	38.89	7.93	2259	32	25	27	7.79	24.62	853.75	856.49	2103.62	130.76	2418.10	2.11	3.23
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	11	Ambient	45000	10.59	7.99	4.77	5.16	45.10	6.62	3.45	44.10	5.37	5.06		7.93	2112	32	25	27	7.79	23.05	799.37	801.93	1966.86	122.08	2263.93	1.97	3.01
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	12	Ambient	67500	14.81	7.23	6.92	8.74	40.12	9.82	6.34	70.83	19.83	5.23		7.93	2083	32	25	27	7.79	22.65	785.24	787.76	1939.51	120.85	2234.28	1.95	2.98
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	13	Ambient	92500	20.45	12.01	3.44	9.66	47.36	3.77	4.28	49.57	3.57	4.46		7.97	2231	32	25	27	7.83	22.11	766.71	769.17	2068.16	140.73	2406.95	2.27	3.47
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	14	Ambient	185000	30.37	6.94			42.40	5.14	4.97	73.12	4.04	4.58	24.71	8.15	2049	32	25	27	8.01	12.99	450.31	451.75	1845.26	190.75	2308.34	3.08	4.71
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	15	Ambient	170000	49.26	6.34	8.31	6.79	41.96	5.01	4.42	59.14	3.41	5.24	28.98	7.97	2140	32	25	27	7.83	21.17	733.94	736.29	1983.59	135.24	2311.50	2.18	3.34
laboratory	Nitzschia closterium (phytoplankton)	573506	marinespecies.org	16	Ambient	365000	62.96	8.75	4.91	9.29	55.93	5.76	6.55	109.06	8.34	5.56	35.61	8.14	2242	32	25	27	8.00	14.60	506.21	507.83	2023.38	204.03	2511.36	3.30	5.03