Citation:

j.marpolbul.2018.06.024

Name: Seawater carbonate chemistry and growth of the dinoflagellate Alexandrium tamarense and its toxicity to zebrafish (Danio rerio) embryos
Published: 2018
License: https://creativecommons.org/licenses/by/4.0/
Keywords: Alexandrium tamarense; Animalia; Behaviour; Bottles or small containers/Aquaria (<20 L); Chromista; Danio rerio; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; Nekton; Not applicable; Other metabolic rates; Other studied parameter or process; Phytoplankton; Single species; Species interaction

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

The effects and interactive effects of different nitrogen (N) sources (ammonium, nitrate, and urea) and carbon dioxide (CO2) concentrations were investigated on Alexandrium tamarense, a harmful marine dinoflagellate, by measuring its growth (μ), extracellular carbonic anhydrase (CA), and its toxicity to zebrafish (Danio rerio) embryo. The μ and CA were influenced more strongly by CO2 concentrations rather than by N sources; significant effects of CO2 on μ and CA were observed under low CO2 concentration (LC) conditions compared to high CO2 concentration (HC) conditions. The ammonium and nitrate media under LC conditions had the maximum μ and CA, which was inhibited under HC conditions. The embryotoxic effects were influenced more strongly by the N sources than by CO2 concentrations, thus excluding the lower deformation in urea under HC conditions. Moreover, the antioxidant enzymes superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione S-transferase (GST), and catalase (CAT) were detected in normal (untreated) zebrafish embryos, and among them, the level of SOD was the highest. In summary, this study provides a clear insight for understanding the effects and interactive effects of N sources and CO2 concentrations on the growth and toxicity of harmful dinoflagellates.

Keyword(s):

Alexandrium tamarense; Animalia; Behaviour; Bottles or small containers/Aquaria (<20 L); Chromista; Danio rerio; Growth/Morphology; Laboratory experiment; Laboratory strains; Macro-nutrients; Nekton; Not applicable; Other metabolic rates; Other studied parameter or process; Phytoplankton; Single species; Species interaction

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Hagens, Mathilde; Hofmann, Andreas; Mueller, Jens-Daniel; Proye, Aurélien; Rae, James; Soetaert, Karline (2019): seacarb: seawater carbonate chemistry with R. R package version 3.2.12. https://CRAN.R-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2019-05-10.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Guan, WanChun		study
2	Species	Species		Guan, WanChun
3	Registration number of species	Reg spec no		Guan, WanChun
4	Uniform resource locator/link to reference	URL ref		Guan, WanChun		WoRMS Aphia ID
5	Treatment	Treat		Guan, WanChun
6	Treatment	Treat		Guan, WanChun
7	Experiment duration	Exp duration	days	Guan, WanChun
8	Cell density	Cells	#/ml	Guan, WanChun
9	Cell density, standard deviation	Cells std dev	±	Guan, WanChun
10	pH	pH		Guan, WanChun		NBS scale
11	pH, standard deviation	pH std dev	±	Guan, WanChun		NBS scale
12	Growth rate	µ	1/day	Guan, WanChun
13	Growth rate, standard deviation	µ std dev	±	Guan, WanChun
14	Extracellular carbonic anhydrase activity, per cell	eCA act/cell	U/#	Guan, WanChun
15	Extracellular carbonic anhydrase activity, standard deviation	eCA act std dev	±	Guan, WanChun
16	Coagulation rate	Coagulation	%	Guan, WanChun
17	Coagulation rate, standard deviation	Coagulation std dev	±	Guan, WanChun
18	Percentage	Perc	%	Guan, WanChun		Rates of tail separation
19	Percentage, standard deviation	Perc std dev	±	Guan, WanChun		Rates of tail separation
20	Deformation rate	Deformation	%	Guan, WanChun
21	Deformation rate, standard deviation	Deformation std dev	±	Guan, WanChun
22	Movement rate	Movement	%	Guan, WanChun
23	Movement rate , standard deviation	Movement std dev	±	Guan, WanChun
24	Retardation rate	Retardation	%	Guan, WanChun
25	Retardation rate, standard deviation	Retardation std dev	±	Guan, WanChun
26	Percentage	Perc	%	Guan, WanChun		Normal development rate of the circulatory system
27	Percentage, standard deviation	Perc std dev	±	Guan, WanChun		Normal development rate of the circulatory system
28	Percentage	Perc	%	Guan, WanChun		Deformation rate of the circulatory system
29	Percentage, standard deviation	Perc std dev	±	Guan, WanChun		Deformation rate of the circulatory system
30	pH	pH		Guan, WanChun		NBS scale
31	pH, standard deviation	pH std dev	±	Guan, WanChun		NBS scale
32	Carbon, inorganic, dissolved	DIC	µmol/kg	Guan, WanChun
33	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Guan, WanChun
34	Carbon, inorganic, dissolved	DIC	µmol/kg	Guan, WanChun
35	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Guan, WanChun
36	Bicarbonate ion	[HCO3]-	µmol/kg	Guan, WanChun
37	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Guan, WanChun
38	Carbon dioxide	CO2	µmol/kg	Guan, WanChun
39	Carbon dioxide, standard deviation	CO2 std dev	±	Guan, WanChun
40	Temperature, water	Temp	°C	Guan, WanChun
41	Salinity	Sal		Guan, WanChun
42	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
44	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 4.0 International (CC-BY-4.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3696 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 Treat	7 Exp duration [days]	8 Cells [#/ml]	9 Cells std dev [±]	10 pH (NBS scale)	11 pH std dev [±] (NBS scale)	12 µ [1/day]	13 µ std dev [±]	14 eCA act/cell [U/#]	15 eCA act std dev [±]	16 Coagulation [%]	17 Coagulation std dev [±]	18 Perc [%] (Rates of tail separation)	19 Perc std dev [±] (Rates of tail separation)	20 Deformation [%]	21 Deformation std dev [±]	22 Movement [%]	23 Movement std dev [±]	24 Retardation [%]	25 Retardation std dev [±]	26 Perc [%] (Normal development rate of th...)	27 Perc std dev [±] (Normal development rate of th...)	28 Perc [%] (Deformation rate of the circu...)	29 Perc std dev [±] (Deformation rate of the circu...)	30 pH (NBS scale)	31 pH std dev [±] (NBS scale)	32 DIC [µmol/kg]	33 DIC std dev [±]	34 DIC [µmol/kg]	35 DIC std dev [±]	36 [HCO3]- [µmol/kg]	37 [HCO3]- std dev [±]	38 CO2 [µmol/kg]	39 CO2 std dev [±]	40 Temp [°C]	41 Sal	42 CSC flag	43 pH (total scale)	44 CO2 [µmol/kg]	45 fCO2water_SST_wet [µatm]	46 pCO2water_SST_wet [µatm]	47 [HCO3]- [µmol/kg]	48 [CO3]2- [µmol/kg]	49 AT [µmol/kg]	50 Omega Arg	51 Omega Cal
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Nitrate	0	4200.00	81.65	8.20000	0.00000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Nitrate	1	4512.50	198.04	8.09000	0.01000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Nitrate	2	6050.00	111.80	8.10500	0.03500																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Nitrate	3	7437.50	102.32	8.14000	0.00000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Nitrate	4	8400.00	196.85	8.13000	0.00000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Nitrate	5	9825.00	529.74	8.21000	0.02000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Nitrate	6	10062.50	238.16	8.09500	0.01500																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Nitrate	7	9025.00	167.71	8.15000	0.05000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Nitrate	8	8837.50	1892.87	8.11000	0.03000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Ammonia	0	4066.67	84.98	8.20000	0.00000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Ammonia	1	4962.50	768.42	8.09500	0.00500																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Ammonia	2	7162.50	974.28	8.09500	0.00500																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Ammonia	3	8637.50	1543.69	8.19000	0.05000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Ammonia	4	10412.50	1345.07	8.11500	0.00500																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Ammonia	5	10337.50	276.98	8.16500	0.00500																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Ammonia	6	9325.00	341.87	8.11000	0.02000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Ammonia	7	8875.00	952.30	8.11000	0.00000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Ammonia	8	8575.00	838.53	8.09000	0.00000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Urea	0	4150.00	40.82	8.20000	0.00000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Urea	1	4450.00	379.14	8.06000	0.00000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Urea	2	5275.00	918.22	8.05500	0.00500																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Urea	3	6012.50	648.44	8.10000	0.02000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Urea	4	6375.00	1602.54	8.11000	0.02000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Urea	5	6150.00	1078.77	8.08500	0.00500																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Urea	6	5550.00	1477.75	8.09500	0.00500																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Urea	7	5575.00	1278.92	8.08000	0.00000																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Urea	8	5237.50	1322.58	8.07500	0.00500																			8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Nitrate	0	4050.00	50.00	7.80000	0.00000																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Nitrate	1	4460.00	201.00	7.50000	0.09416																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Nitrate	2	4670.00	385.49	7.52667	0.02055																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Nitrate	3	6525.00	453.46	7.58667	0.06342																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Nitrate	4	7141.67	292.14	7.66667	0.02055																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Nitrate	5	7758.33	330.93	7.86000	0.00816																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Nitrate	6	7575.00	688.45	7.76333	0.00471																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Nitrate	7	6116.67	379.33	7.55000	0.00000																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Nitrate	8	5550.00	40.82	7.61000	0.00000																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Ammonia	0	4000.00	100.00	7.80000	0.00000																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Ammonia	1	4641.67	233.48	7.49000	0.02944																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Ammonia	2	5150.00	298.61	7.54333	0.03300																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Ammonia	3	5641.67	376.85	7.73000	0.02944																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Ammonia	4	6558.33	354.04	7.71667	0.00943																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Ammonia	5	6933.33	405.86	7.85333	0.01700																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Ammonia	6	7133.33	364.77	7.77667	0.01247																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Ammonia	7	6525.00	394.49	7.65000	0.00000																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Ammonia	8	6283.33	94.28	7.59000	0.00000																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Urea	0	4316.67	131.23	7.80000	0.00000																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Urea	1	4741.67	462.26	7.52667	0.02494																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Urea	2	5333.33	778.17	7.60333	0.01886																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Urea	3	5950.00	647.43	7.76000	0.02160																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Urea	4	7308.33	870.07	7.76333	0.00471																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Urea	5	7600.00	774.60	7.88333	0.01247																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Urea	6	7175.00	1098.77	7.76667	0.01247																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Urea	7	5433.33	249.44	7.62000	0.00000																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Urea	8	6125.00	125.00	7.62000	0.00000																			7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	nitrate						0.19475	0.01074	209958	24353															8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Ammonia						0.25107	0.05193	209036	19236															8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	Low CO2	Urea						0.12104	0.02841	116310	63569															8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	nitrate						0.12709	0.00649	91072	14103															7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Ammonia						0.10966	0.01181	85553	57912															7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Alexandrium tamarense (phytoplankton)	109714	marinespecies.org	High CO2	Urea						0.11208	0.02063	76867	11797															7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			Low CO2	Nitrate		0.00								0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Nitrate		5000.00								8.33333	7.21688	91.67000	7.21688	8.33333	7.21688	91.66667	7.21688	33.33333	7.21688	91.66667	7.21688	8.33333	5.89256	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Nitrate		10000.00								8.33333	7.21688	87.50000	12.50000	16.66667	19.09407	87.50000	12.50000	33.33333	7.21688	87.50000	12.50000	12.50000	10.20621	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Nitrate		20000.00								12.50000	12.50000	75.00000	12.50000	20.83333	7.21688	83.33333	7.21688	37.50000	12.50000	83.33333	7.21688	20.83333	5.89256	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Nitrate		40000.00								20.83333	7.21688	54.17000	14.43376	45.83333	31.45764	66.66667	19.09407	25.00000	0.00000	66.66667	19.09407	37.50000	10.20621	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Nitrate		80000.00								83.33333	14.43376	4.17000	7.21688	91.66667	14.43376	8.33333	14.43376	4.16667	7.21688	8.33333	14.43376	91.66667	11.78511	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Ammonia		0.00								0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Ammonia		5000.00								0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Ammonia		10000.00								0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	96.66667	0.57735	0.00000	0.00000	96.66667	0.57735	3.33333	4.71405	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Ammonia		20000.00								3.33333	5.77350	90.00000	10.00000	3.33333	5.77350	90.00000	0.00000	6.66667	5.77350	96.66667	5.77350	6.66667	9.42809	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Ammonia		40000.00								3.33333	5.77350	43.33333	5.77350	3.33333	5.77350	83.33333	5.77350	23.33333	5.77350	96.66667	5.77350	16.66667	9.42809	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Ammonia		80000.00								10.00000	1.00000	13.33333	1.15470	10.00000	1.00000	73.33333	0.57735	43.33333	0.57735	90.00000	1.00000	20.00000	8.16497	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Urea		0.00								0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Urea		5000.00								6.66667	5.77350	93.33333	5.77350	6.66667	5.77350	93.33333	5.77350	0.00000	0.00000	93.33333	5.77350	6.66667	4.71405	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Urea		10000.00								6.66667	5.77350	90.00000	0.00000	10.00000	0.00000	90.00000	0.00000	6.66667	11.54701	90.00000	0.00000	10.00000	0.00000	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Urea		20000.00								13.33333	5.77350	70.00000	0.00000	13.33333	5.77350	86.66667	5.77350	23.33333	5.77350	86.66667	5.77350	20.00000	8.16497	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Urea		40000.00								20.00000	0.00000	60.00000	10.00000	20.00000	0.00000	80.00000	0.00000	33.33333	5.77350	80.00000	0.00000	30.00000	8.16497	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Urea		80000.00								26.66667	5.77350	50.00000	0.00000	26.66667	5.77350	73.33333	5.77350	46.66667	5.77350	66.66667	11.54701	60.00000	0.00000	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			High CO2	Nitrate		0.00								0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Nitrate		5000.00								12.50000	12.50000	83.33333	7.21688	12.50000	12.50000	87.50000	12.50000	37.50000	0.00000	87.50000	12.50000	12.50000	10.20621	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Nitrate		10000.00								16.66667	7.21688	83.33333	7.21688	16.66667	7.21688	83.33333	7.21688	29.16667	7.21688	83.33333	7.21688	16.66667	5.89256	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Nitrate		20000.00								20.83333	7.21688	79.16667	7.21688	20.83333	7.21688	79.16667	7.21688	29.16667	7.21688	79.16667	7.21688	20.83333	5.89256	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Nitrate		40000.00								20.83333	7.21688	62.50000	12.50000	25.00000	12.50000	75.00000	12.50000	20.83333	7.21688	70.83333	7.21688	29.16667	5.89256	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Nitrate		80000.00								54.16667	19.09407	8.33333	7.21688	66.66667	19.09407	33.33333	19.09407	33.33333	19.09407	33.33333	19.09407	73.33333	12.47219	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Ammonia		0.00								0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Ammonia		5000.00								0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Ammonia		10000.00								3.33333	0.57735	96.66667	0.57735	3.33333	0.57735	96.66667	0.57735	0.00000	0.00000	96.66667	0.57735	3.33333	4.71405	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Ammonia		20000.00								6.66667	11.54701	83.33333	11.54701	6.66667	11.54701	86.66667	5.77350	6.66667	5.77350	93.33333	11.54701	6.66667	9.42809	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Ammonia		40000.00								6.66667	11.54701	46.66667	5.77350	6.66667	11.54701	76.66667	5.77350	26.66667	5.77350	93.33333	11.54701	6.66667	9.42809	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Ammonia		80000.00								10.00000	1.00000	6.66667	0.57735	10.00000	1.00000	66.66667	0.57735	40.00000	1.00000	90.00000	1.00000	16.66667	4.71405	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Urea		0.00								0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	100.00000	0.00000	0.00000	0.00000	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Urea		5000.00								6.66667	5.77350	90.00000	10.00000	6.66667	5.77350	93.33333	5.77350	0.00000	0.00000	93.33333	5.77350	6.66667	4.71405	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Urea		10000.00								10.00000	10.00000	76.66667	15.27525	10.00000	10.00000	90.00000	10.00000	10.00000	17.32051	90.00000	10.00000	10.00000	8.16497	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Urea		20000.00								13.33333	5.77350	73.33333	5.77350	13.33333	5.77350	86.66667	5.77350	26.66667	5.77350	86.66667	5.77350	13.33333	4.71405	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Urea		40000.00								20.00000	10.00000	66.66667	5.77350	20.00000	10.00000	83.33333	5.77350	40.00000	10.00000	83.33333	5.77350	16.66667	4.71405	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Urea		80000.00								33.33333	5.77350	46.66667	5.77350	33.33333	5.77350	66.66667	5.77350	46.66667	15.27525	63.33333	5.77350	36.66667	4.71405	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			Low CO2	nitrate		80000.00								83.33333	14.43376	4.17000	7.21688	91.66667	14.43376	8.33333	14.43376	4.16667	7.21688	8.33333	14.43376	91.66667	11.78511	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Ammonia		80000.00								10.00000	1.00000	13.33333	1.15470	10.00000	1.00000	73.33333	0.57735	43.33333	0.57735	90.00000	1.00000	20.00000	8.16497	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			Low CO2	Urea		80000.00								26.66667	5.77350	50.00000	0.00000	26.66667	5.77350	73.33333	5.77350	46.66667	5.77350	66.66667	11.54701	60.00000	0.00000	8.12	0.05	2405.60	33.9	2170.0	11.0	222.1	26.4	13.5	1.5	20	22	27	7.98	21.27	611.68	613.77	2247.28	137.05	2562.48	2.22	3.59
laboratory	Danio rerio (fish)			High CO2	nitrate		80000.00								54.16667	19.09407	8.33333	7.21688	66.66667	19.09407	33.33333	19.09407	33.33333	19.09407	33.33333	19.09407	73.33333	12.47219	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Ammonia		80000.00								10.00000	1.00000	6.66667	0.57735	10.00000	1.00000	66.66667	0.57735	40.00000	1.00000	90.00000	1.00000	16.66667	4.71405	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36
laboratory	Danio rerio (fish)			High CO2	Urea		80000.00								33.33333	5.77350	46.66667	5.77350	33.33333	5.77350	66.66667	5.77350	46.66667	15.27525	63.33333	5.77350	36.66667	4.71405	7.69	0.13	2400.03	101.1	2272.5	91.1	94.0	14.7	33.6	4.7	20	22	27	7.55	58.35	1678.29	1684.02	2289.82	51.86	2410.21	0.84	1.36