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Franco-Santos, Rita Melo; Auel, Holger; Boersma, Maarten; De Troch, Marleen; Graeve, Martin; Meunier, Cédric Léo; Niehoff, Barbara (2019): Fatty acid contents for the larvae of the polychaete Lanice conchilega [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.900109, In supplement to: Franco-Santos, RM et al. (2019): You are not always what you eat—Fatty acid bioconversion and lipid homeostasis in the larvae of the sand mason worm Lanice conchilega. PLoS ONE, 14(6), e0218015, https://doi.org/10.1371/journal.pone.0218015

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Abstract:
The larvae of the reef-building polychaete Lanice conchilega can make up to 15% of the summer zooplankton biomass in the North Sea. Despite their importance for reef maintenance (which positively affects the benthic community), little is known about the trophic ecology of this meroplanktonic larva. Qualitative and quantitative estimates of carbon (C) transfer between trophic levels and of fatty acid (FA)-specific assimilation, biosynthesis, and bioconversion can be obtained by compound-specific stable isotope analysis of FA. The present work tested the hypothesis that the concept of fatty acid trophic markers (FATM), widely used for studies on holoplankton with intermediate to high lipid contents, is also applicable to lipid-poor organisms such as meroplanktonic larvae. The incorporation of isotopically-enriched dietary C by L. conchilega larvae was traced, and lipid assimilation did not follow FA-specific relative availabilities in the diet. Furthermore, FAs that were unavailable in the diet, such as 22:5(n-3), were recorded in L. conchilega, suggesting their bioconversion by the larvae. The results indicate that L. conchilega larvae preferentially assimilate certain FAs and regulate their FA composition (lipid homeostasis) independently of that of their diet. Their quasi-homeostatic response to dietary FA availability could imply that the concept of FATM has limited application in lipid-poor organisms such as L. conchilega larvae.
Keyword(s):
assimilation; bioconversion; compound-specific stable isotope analysis; fatty acid trophic markers; lipid-poor species; meroplanktonic larvae; polychaete
Coverage:
Latitude: 54.183333 * Longitude: 7.900000
Date/Time Start: 2016-06-06T17:30:00 * Date/Time End: 2016-06-11T21:00:00
Minimum Elevation: -10.0 m * Maximum Elevation: -10.0 m
Event(s):
HelgolandRoads_larvae_2016 (Kabeltonne) * Latitude: 54.188330 * Longitude: 7.900000 * Date/Time Start: 2016-06-06T17:10:00 * Date/Time End: 2016-06-11T21:00:00 * Elevation: -10.0 m * DEIMS ID: deims.org * Location: German Bight, North Sea
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1SpeciesSpeciesFranco-Santos, Rita Melo
2LATITUDELatitudeFranco-Santos, Rita MeloGeocode – Sampling
3LONGITUDELongitudeFranco-Santos, Rita MeloGeocode – Sampling
4DATE/TIMEDate/TimeFranco-Santos, Rita MeloGeocode – Sampling
5CommentCommentFranco-Santos, Rita MeloSampling time within experiment
6Day of experimentDOEdayFranco-Santos, Rita Melot
7SpeciesSpeciesFranco-Santos, Rita MeloFood item
8ReplicateReplFranco-Santos, Rita Melo
9Fatty acid, common nameFA commonFranco-Santos, Rita Melo
10Fatty acid, n-x nomenclatureFA A:B(n-x)Franco-Santos, Rita MeloA: number of carbon atoms: B: number of double bonds; (n-X): position of the double bond closest to the terminal methyl group
11Fatty acid, IUPAC nomenclatureFA IUPACFranco-Santos, Rita Melo
12Fatty acids, δ13CFA δ13C‰ PDBFranco-Santos, Rita MeloRelative to VPDBδ 13C (‰) = [((13Csample/12Csample)/(13CVPDBstandard/12CVPDBstandard)) -1] * 1000
13Atom percentAt perc%Franco-Santos, Rita MeloAtom% = ((13Csample/12Csample)/((13Csample/12Csample)+1)) * 100
14Atom percent excessAPE%Franco-Santos, Rita MeloAPE= atom% experimental polychaete - (average atom% in situ polychaetes)
15Fatty acid contentFA conµgFranco-Santos, Rita MeloIn sample
16Polychaeta, larvaePolychaeta larv#Franco-Santos, Rita MeloNumber of polychaete larvae in the sample
17Fatty acid per larvaeFA larvµg/#Franco-Santos, Rita MeloFAmass = Mass FA sample / #
18Fatty acid as percentage of total fatty acidsFA%Franco-Santos, Rita Melo%TFA = (mass FA sample * 100) / ∑(mass all FAs sample)
19Carbon, number of atomsC atoms#Franco-Santos, Rita MeloNumber of atoms in the FA molecule (CFA)
20Number of insaturationsN insatur#Franco-Santos, Rita MeloNumber of insaturations in the FA molecule (M)
21Hydrogen, number of atomsH atoms#Franco-Santos, Rita MeloNumber of atoms in the FA molecule (HFA,=2CFA-2M)
22Carbon, number of atomsC atoms#Franco-Santos, Rita MeloNumber of atoms in the FAME molecule (CFAME)
23Number of insaturationsN insatur#Franco-Santos, Rita MeloNumber of insaturations in the FAME molecule (M)
24Hydrogen, number of atomsH atoms#Franco-Santos, Rita MeloNumber of atoms in the FAME molecule (HFAME, =2CFAME-2M)
25Oxygen, number of atomsO atoms#Franco-Santos, Rita MeloNumber of atoms in the FAME molecule
26Atomic weightAtomic weightuFranco-Santos, Rita Melo13C (A13C)
27Atomic weightAtomic weightuFranco-Santos, Rita Melo12C (A12C)
28Atomic weightAtomic weightuFranco-Santos, Rita MeloH (AH)
29Atomic weightAtomic weightuFranco-Santos, Rita MeloO (AO)
30Fatty acid as carbon per larvaeFA C larvµg/#Franco-Santos, Rita MeloCmass = (((atom% / 100*A13C) + ((100-atom%) / 100* A12C)) * CFA * FAmass) / (((atom% / 100*A13C) + ((100-atom%) / 100* A12C)) * CFA) + A12C + (HFAME * AH) + (OFAME * AO)
31Carbon, proportion of assimilatedC prop assimFranco-Santos, Rita MeloPA = APE / average atom% in algae FA (L) (use L = 3.62% for all FAs but 18:0, and L = 1.87% for FA 18:0)
32Carbon assimilationC assimµg/#Franco-Santos, Rita MeloCassim = Cmass * PA
33Turnover rate, carbonCt%/dayFranco-Santos, Rita MeloCT = (Cassim / Cmass) / Δt
Change history:
2023-07-06T08:26:19 – Event label was replaced. New event contains more information.
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
2441 data points

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