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Rebelein, Anja; Bock, Christian (2018): Metabolite profiles of two Antarctic sister species (Notothenia rossii and Notothenia coriiceps) at different temperatures. PANGAEA, https://doi.org/10.1594/PANGAEA.896853, Supplement to: Rebelein, Anja; Pörtner, Hans-Otto; Bock, Christian (2018): Untargeted metabolic profiling reveals distinct patterns of thermal sensitivity in two related notothenioids. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 217, 43-54, https://doi.org/10.1016/j.cbpa.2017.12.012

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Abstract:
Antarctic marine ectothermal animals may be affected more than temperate species by rising ocean water temperatures due to ongoing climate change. Their specialisation on stable cold temperatures make them vulnerable to even small degrees of warming. Thus, addressing the impacts of warming on Antarctic organisms and identifying their potentially limited capacities to respond is of particular interest.
The objective of the study was to determine changes in metabolite profiles related to temperature exposure and acclimation. In a long-term experiment adult fish of two Antarctic sister species Notothenia rossii and Notothenia coriiceps were acclimated to 0 °C and 5 °C for three months. Impacts and indicators of acclimation at the cellular level were determined from metabolite profiles quantified in gill tissue extracts by using nuclear magnetic resonance (NMR) spectroscopy. Furthermore, the metabolite profiles of the two con-generic species were compared.
NMR spectroscopy identified over 40 metabolites that were present in each sample, but varied in their absolute concentration between species and between treatments. Variations in the concentration of phospholipid compounds, amino acids and osmolytes suggested that warming caused changes in the cellular membrane structure. It also increased the catabolism of amino acids and initiated shifts in osmoregulation. Some differences in the metabolite profile between the two notothenioid species were related to their divergent lifestyles, especially their different rates of motor activity. Increased levels of the Krebs cycle intermediate succinate and falling amino acid levels in warm-acclimated N. rossii suggested that N. rossii is more sensitive to warming than N. coriiceps.
Data are obtained from dried polar gill extracts, which were re-suspended in deuterated water (D2O) to a final concentration of 1 g frozen gill tissue/ml solvent. The D2O contained 0.05 wt.% of 3-(trimethylsilyl) propionic-2,2,3,3-d4 acid, sodium salt (TSP) (Sigma Aldrich, St. Louis, USA). TSP was used as chemical shift and quantification standard. For each sample 50 µl of the resuspended gill extracts were analysed.
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