Haider, Fouzia; Sokolov, Eugene; Timm, Stefan; Hagemann, Martin; Blanco-Rayon, Esther; Marigomez, Ionan; Izagirre, Urtzi; Sokolova, Inna M (2018): Effect of salinity and burial on proteasomal activity in Mya arenaria [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.895670, In supplement to: Haider, F et al. (2019): Interactive effects of osmotic stress and burrowing activity on protein metabolism and muscle capacity in the soft shell clam Mya arenaria. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 228, 81-93, https://doi.org/10.1016/j.cbpa.2018.10.022
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Abstract:
Bioturbators (such as bivalves, worms, polychaetes), living in a coastal area, experiences frequent changes in salinity. They are often exposed to mechanical disturbances (like wave, currents, storms) forcing them to bury deeper into the sediment to get a better foothold. In nature, these stressors often occurs simultaneously. Osmotic stress negatively affects the burial activity and the physiological performances of soft shell clam, Mya arenaria; however, the mechanism behind this is still unknown. In this dataset we present the combined effect of osmotic stress and repeated burrowing on the trypsin- and chymotrypsin-like proteasomal and lysosomal activity in gill tissue and digestive gland of Mya arenaria.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Regime | Regime | Haider, Fouzia | Acclimated Salinity Regime | ||
| 2 | Salinity | Sal | Haider, Fouzia | while exercising, in PSU | ||
| 3 | Group | Group | Haider, Fouzia | |||
| 4 | Sample ID | Sample ID | Haider, Fouzia | |||
| 5 | Mya arenaria, shell length | M. arenaria shell l | mm | Haider, Fouzia | ||
| 6 | Number | n | # | Haider, Fouzia | of times the animal was disturbed | |
| 7 | Tissues | Tissues | Haider, Fouzia | |||
| 8 | Trypsin-like proteasomal activity, unit per tissue mass | Trypsin-like proteasomal act/tis | U/mg | Haider, Fouzia | ||
| 9 | Trypsin-like lysosomal activity, unit per tissue mass | Trypsin-like lysosomal act/tis | U/mg | Haider, Fouzia | ||
| 10 | Chymotrypsin-like proteasomal activity, unit per tissue mass | Chymotrypsin-like proteasomal act/tis | U/mg | Haider, Fouzia | ||
| 11 | Chymotrypsin-like lysosomal activity, unit per tissue mass | Chymotrypsin-like lysosomal act/tis | U/mg | Haider, Fouzia | ||
| 12 | Comment | Comment | Haider, Fouzia | Outlier |
License:
Creative Commons Attribution 4.0 International (CC-BY-4.0)
Size:
982 data points
Data
| 1 Regime | 2 Sal | 3 Group | 4 Sample ID | 5 M. arenaria shell l [mm] | 6 n [#] | 7 Tissues | 8 Trypsin-like proteasomal act/tis [U/mg] | 9 Trypsin-like lysosomal act/tis [U/mg] | 10 Chymotrypsin-like proteasomal act/tis [U/mg] | 11 Chymotrypsin-like lysosomal act/tis [U/mg] | 12 Comment |
|---|---|---|---|---|---|---|---|---|---|---|---|
| Low | 5 | Control | LGC1 | 45 | 0 | Gill | 5.78 | 13.86 | 10.58 | 5.07 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin, Proteasome-Chymotrypsin |
| Low | 5 | Control | LGC2 | 50 | 0 | Gill | 20.18 | 12.61 | 3.14 | 9.27 | |
| Low | 5 | Control | LGC3 | 52 | 0 | Gill | 10.33 | 11.84 | 4.86 | 3.99 | |
| Low | 5 | Control | LGC4 | 50 | 0 | Gill | 6.27 | 13.17 | 5.46 | 5.50 | |
| Low | 5 | Control | LGC5 | 54 | 0 | Gill | 14.19 | 11.62 | 4.68 | 6.99 | |
| Low | 5 | Control | LGC6 | 53 | 0 | Gill | 30.77 | 11.56 | 4.71 | 9.37 | |
| Low | 5 | Control | LGC7 | 42 | 0 | Gill | 16.00 | 14.18 | 4.06 | 5.54 | |
| Low | 5 | Exhausted | LGE1 | 25 | 4 | Gill | 9.24 | 6.25 | 2.99 | 2.05 | |
| Low | 5 | Exhausted | LGE2 | 32 | 4 | Gill | 21.28 | 20.26 | 4.35 | 2.47 | |
| Low | 5 | Exhausted | LGE3 | 27 | 5 | Gill | 7.92 | 8.46 | 2.13 | 2.07 | |
| Low | 5 | Exhausted | LGE4 | 27 | 5 | Gill | 8.93 | 7.74 | 2.74 | 1.99 | |
| Low | 5 | Exhausted | LGE5 | 28 | 5 | Gill | 21.20 | 11.66 | 3.48 | 2.83 | |
| Low | 5 | Exhausted | LGE10 | 29 | 5 | Gill | 9.65 | 7.36 | 2.79 | 2.03 | |
| Low | 5 | Exhausted | LGE11 | 40 | 5 | Gill | 17.64 | 8.02 | 6.21 | 4.43 | |
| Normal | 15 | Control | NGC1 | 48 | 0 | Gill | 14.60 | 18.22 | 3.72 | 4.24 | |
| Normal | 15 | Control | NGC2 | 50 | 0 | Gill | 24.91 | 20.85 | 5.04 | 5.20 | |
| Normal | 15 | Control | NGC3 | 58 | 0 | Gill | 22.08 | 13.00 | 3.68 | 10.10 | |
| Normal | 15 | Control | NGC4 | 50 | 0 | Gill | 0.90 | 8.50 | 1.67 | 2.59 | |
| Normal | 15 | Control | NGC5 | 35 | 0 | Gill | 17.64 | 19.12 | 3.25 | 5.31 | |
| Normal | 15 | Control | NGC6 | 43 | 0 | Gill | 14.66 | 15.56 | 1.68 | 4.99 | |
| Normal | 15 | Control | NGC8 | 38 | 0 | Gill | 23.28 | 22.91 | 2.87 | 7.42 | |
| Normal | 15 | Control | NGC10 | 34 | 0 | Gill | 18.47 | 12.49 | 5.88 | 8.06 | |
| Normal | 15 | Exhausted | NGE1 | 32 | 6 | Gill | 17.79 | 14.23 | 2.96 | 6.56 | |
| Normal | 15 | Exhausted | NGE2 | 25 | 7 | Gill | 7.05 | 11.87 | 1.37 | 2.32 | |
| Normal | 15 | Exhausted | NGE4 | 25 | 9 | Gill | 12.58 | 16.34 | 2.18 | 4.87 | |
| Normal | 15 | Exhausted | NGE6 | 29 | 10 | Gill | 13.42 | 20.24 | 2.91 | 5.38 | |
| Normal | 15 | Exhausted | NGE8 | 27 | 11 | Gill | 22.57 | 22.17 | 1.48 | 7.93 | |
| Normal | 15 | Exhausted | NGE10 | 38 | 6 | Gill | 37.26 | 30.80 | 3.95 | 8.75 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin, Proteasome-Chymotrypsin |
| Normal | 15 | Exhausted | NGE12 | 29 | 11 | Gill | 27.15 | 22.50 | 3.36 | 6.06 | |
| Fluctuating | 15 | Control | FGC1 | 35 | 0 | Gill | 10.69 | 38.97 | 2.54 | 10.81 | |
| Fluctuating | 15 | Control | FGC2 | 43 | 0 | Gill | 49.36 | 32.07 | 5.81 | 6.70 | |
| Fluctuating | 15 | Control | FGC3 | 43 | 0 | Gill | 27.03 | 29.56 | 10.58 | 7.66 | |
| Fluctuating | 15 | Control | FGC5 | 39 | 0 | Gill | 29.84 | 27.43 | 8.65 | 7.47 | |
| Fluctuating | 15 | Control | FGC6 | 44 | 0 | Gill | 42.19 | 38.70 | 7.58 | 13.64 | |
| Fluctuating | 15 | Control | FGC7 | 40 | 0 | Gill | 61.56 | 32.97 | 15.42 | 17.14 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin, Proteasome-Chymotrypsin |
| Fluctuating | 15 | Control | FGC8 | 40 | 0 | Gill | 31.09 | 31.48 | 7.30 | 7.61 | |
| Fluctuating | 15 | Exhausted | FGE1 | 30 | 5 | Gill | 60.14 | 29.17 | 12.49 | 13.55 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin, Proteasome-Chymotrypsin |
| Fluctuating | 15 | Exhausted | FGE3 | 30 | 6 | Gill | 40.62 | 39.98 | 11.14 | 9.97 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin, Proteasome-Chymotrypsin |
| Fluctuating | 15 | Exhausted | FGE5 | 29 | 7 | Gill | 23.70 | 17.98 | 8.17 | 8.97 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin, Proteasome-Chymotrypsin |
| Fluctuating | 15 | Exhausted | FGE6 | 31 | 7 | Gill | 19.03 | 20.16 | 4.20 | 7.67 | |
| Fluctuating | 15 | Exhausted | FGE7 | 34 | 7 | Gill | 28.32 | 20.94 | 5.93 | 7.19 | |
| Fluctuating | 15 | Exhausted | FGE10 | 38 | 6 | Gill | 22.44 | 26.81 | 10.95 | 13.06 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin, Proteasome-Chymotrypsin |
| Fluctuating | 15 | Exhausted | FGE11 | 28 | 5 | Gill | 38.83 | 27.45 | 6.92 | 5.99 | |
| Fluctuating | 15 | Exhausted | FGE12 | 28 | 9 | Gill | 25.98 | 28.40 | 5.33 | 8.24 | |
| Low | 5 | Control | LHC1 | 45 | 0 | HP | 19.47 | 35.47 | 4.36 | 3.48 | |
| Low | 5 | Control | LHC2 | 50 | 0 | HP | 24.37 | 22.44 | 4.05 | 5.05 | |
| Low | 5 | Control | LHC3 | 52 | 0 | HP | 39.99 | 27.69 | 7.10 | 4.44 | |
| Low | 5 | Control | LHC4 | 50 | 0 | HP | 51.14 | 21.99 | 7.64 | 3.93 | |
| Low | 5 | Control | LHC5 | 54 | 0 | HP | 39.37 | 19.00 | 5.31 | 3.06 | |
| Low | 5 | Control | LHC6 | 53 | 0 | HP | 34.92 | 29.89 | 13.02 | 5.52 | |
| Low | 5 | Control | LHC7 | 42 | 0 | HP | 38.15 | 31.33 | 11.98 | 6.62 | |
| Low | 5 | Exhausted | LHE1 | 25 | 4 | HP | 10.72 | 25.58 | 1.09 | 1.01 | |
| Low | 5 | Exhausted | LHE2 | 32 | 4 | HP | 21.20 | 12.03 | 0.00 | 0.00 | |
| Low | 5 | Exhausted | LHE3 | 27 | 5 | HP | 12.49 | 20.37 | 3.02 | 2.21 | |
| Low | 5 | Exhausted | LHE4 | 27 | 5 | HP | 9.46 | 36.11 | 6.60 | 7.87 | |
| Low | 5 | Exhausted | LHE5 | 28 | 5 | HP | 27.73 | 65.79 | 19.54 | 10.98 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin, Proteasome-Chymotrypsin |
| Low | 5 | Exhausted | LHE10 | 29 | 5 | HP | 21.02 | 23.54 | 3.93 | 2.70 | |
| Low | 5 | Exhausted | LHE11 | 40 | 5 | HP | 20.14 | 22.12 | 6.20 | 3.27 | |
| Normal | 15 | Control | NHC1 | 48 | 0 | HP | 57.92 | 52.39 | 13.00 | 6.60 | Proteasome-Chymotrypsin |
| Normal | 15 | Control | NHC2 | 50 | 0 | HP | 30.33 | 37.68 | 11.84 | 5.90 | Proteasome-Chymotrypsin |
| Normal | 15 | Control | NHC3 | 58 | 0 | HP | 50.97 | 51.37 | 25.15 | 8.53 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin, Proteasome-Chymotrypsin |
| Normal | 15 | Control | NHC4 | 50 | 0 | HP | 44.13 | 50.95 | 12.12 | 2.37 | |
| Normal | 15 | Control | NHC5 | 35 | 0 | HP | 24.27 | 62.44 | 12.02 | 5.55 | |
| Normal | 15 | Control | NHC6 | 43 | 0 | HP | 45.08 | 46.71 | 10.29 | 4.91 | |
| Normal | 15 | Control | NHC8 | 38 | 0 | HP | 29.26 | 59.92 | 8.95 | 6.19 | |
| Normal | 15 | Control | NHC10 | 34 | 0 | HP | 20.73 | 38.22 | 13.12 | 3.99 | |
| Normal | 15 | Exhausted | NHE1 | 32 | 6 | HP | 53.37 | 31.87 | 5.44 | 4.03 | |
| Normal | 15 | Exhausted | NHE2 | 25 | 7 | HP | 47.02 | 26.71 | 4.45 | 3.57 | |
| Normal | 15 | Exhausted | NHE4 | 25 | 9 | HP | 33.07 | 29.43 | 3.56 | 3.66 | |
| Normal | 15 | Exhausted | NHE6 | 29 | 10 | HP | 17.50 | 99.07 | 5.20 | 4.72 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin |
| Normal | 15 | Exhausted | NHE8 | 27 | 11 | HP | 44.02 | 40.52 | 5.34 | 4.17 | |
| Normal | 15 | Exhausted | NHE10 | 38 | 6 | HP | 35.93 | 38.11 | 6.07 | 5.13 | |
| Normal | 15 | Exhausted | NHE12 | 29 | 11 | HP | 36.00 | 37.03 | 5.30 | 4.25 | |
| Fluctuating | 15 | Control | FHC1 | 35 | 0 | HP | 46.61 | 46.64 | 11.73 | 9.05 | |
| Fluctuating | 15 | Control | FHC2 | 43 | 0 | HP | 65.41 | 33.64 | 11.84 | 9.88 | |
| Fluctuating | 15 | Control | FHC3 | 43 | 0 | HP | 49.53 | 44.36 | 7.72 | 8.86 | |
| Fluctuating | 15 | Control | FHC5 | 39 | 0 | HP | 0.00 | 0.00 | 8.65 | 6.90 | Lysosome-Trypsin, Lysosome-Chymotrypsin, Proteasome-Trypsin |
| Fluctuating | 15 | Control | FHC6 | 44 | 0 | HP | 48.67 | 48.06 | 8.08 | 7.29 | |
| Fluctuating | 15 | Control | FHC7 | 40 | 0 | HP | 70.50 | 32.60 | 9.94 | 9.53 | |
| Fluctuating | 15 | Control | FHC8 | 40 | 0 | HP | 61.43 | 27.92 | 10.08 | 6.34 | |
| Fluctuating | 15 | Exhausted | FHE1 | 30 | 5 | HP | 68.21 | 38.21 | 8.09 | 6.72 | |
| Fluctuating | 15 | Exhausted | FHE3 | 30 | 6 | HP | 63.48 | 50.87 | 12.81 | 8.71 | |
| Fluctuating | 15 | Exhausted | FHE5 | 29 | 7 | HP | 31.88 | 27.02 | 4.85 | 3.47 | Proteasome-Chymotrypsin |
| Fluctuating | 15 | Exhausted | FHE6 | 31 | 7 | HP | 73.31 | 19.39 | 6.47 | 4.45 | |
| Fluctuating | 15 | Exhausted | FHE7 | 34 | 7 | HP | 38.40 | 51.60 | 7.72 | 5.53 | |
| Fluctuating | 15 | Exhausted | FHE10 | 38 | 6 | HP | 50.16 | 44.43 | 10.28 | 8.64 | |
| Fluctuating | 15 | Exhausted | FHE11 | 28 | 5 | HP | 66.29 | 39.52 | 14.50 | 6.23 | |
| Fluctuating | 15 | Exhausted | FHE12 | 28 | 9 | HP | 53.92 | 37.69 | 10.01 | 6.80 |