Citation:

s12864-018-4519-y

Name: Seawater carbonate chemistry and gene expression, shell formation in larval Pacific oysters (Crassostrea gigas)
Published: 2018-09-05
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Crassostrea gigas; Gene expression (incl. proteomics); Laboratory experiment; Mollusca; North Pacific; Pelagos; Single species; Temperate; Zooplankton

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Abstract:

Background: Despite recent work to characterize gene expression changes associated with larval development in oysters, the mechanism by which the larval shell is first formed is still largely unknown. In Crassostrea gigas, this shell forms within the first 24 h post fertilization, and it has been demonstrated that changes in water chemistry can cause delays in shell formation, shell deformations and higher mortality rates. In this study, we use the delay in shell formation associated with exposure to CO2-acidified seawater to identify genes correlated with initial shell deposition.

Results: By fitting linear models to gene expression data in ambient and low aragonite saturation treatments, we are able to isolate 37 annotated genes correlated with initial larval shell formation, which can be categorized into 1) ion transporters, 2) shell matrix proteins and 3) protease inhibitors. Clustering of the gene expression data into co-expression networks further supports the result of the linear models, and also implies an important role of dynein motor proteins as transporters of cellular components during the initial shell formation process.

Conclusions: Using an RNA-Seq approach with high temporal resolution allows us to identify a conceptual model for how oyster larval calcification is initiated. This work provides a foundation for further studies on how genetic variation in these identified genes could affect fitness of oyster populations subjected to future environmental changes, such as ocean acidification.

Keyword(s):

Animalia; Bottles or small containers/Aquaria (<20 L); Calcification/Dissolution; Coast and continental shelf; Crassostrea gigas; Gene expression (incl. proteomics); Laboratory experiment; Mollusca; North Pacific; Pelagos; Single species; Temperate; Zooplankton

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-09-04.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		de Wit, Pierre		study
2	Species	Species		de Wit, Pierre
3	Registration number of species	Reg spec no		de Wit, Pierre
4	Uniform resource locator/link to reference	URL ref		de Wit, Pierre		WoRMS Aphia ID
5	Experiment	Exp		de Wit, Pierre
6	Treatment	Treat		de Wit, Pierre
7	Time in hours	Time	h	de Wit, Pierre		time post fertilization
8	Individuals	Ind	#	de Wit, Pierre		count, replicate 1
9	Individuals	Ind	#	de Wit, Pierre		partially calcified, replicate 1
10	Individuals	Ind	#	de Wit, Pierre		fully calcified, replicate 1
11	Individuals	Ind	#	de Wit, Pierre		count, replicate 2
12	Individuals	Ind	#	de Wit, Pierre		partially calcified, replicate 2
13	Individuals	Ind	#	de Wit, Pierre		fully calcified, replicate 2
14	Calcification index	Calc I		de Wit, Pierre
15	Calcification index, standard deviation	Calc I std dev	±	de Wit, Pierre
16	mRNA gene expression, relative	mRNA express		de Wit, Pierre
17	mRNA gene expression, relative, standard deviation	mRNA express std dev	±	de Wit, Pierre
18	mRNA gene expression, relative, standard error	mRNA express std e	±	de Wit, Pierre
19	mRNA gene expression, relative	mRNA express		de Wit, Pierre		Tyrosinase (contig CGI_10007793) expression
20	Temperature, water	Temp	°C	de Wit, Pierre
21	Temperature, water, standard deviation	Temp std dev	±	de Wit, Pierre
22	Salinity	Sal		de Wit, Pierre
23	Salinity, standard deviation	Sal std dev	±	de Wit, Pierre
24	Alkalinity, total	AT	µmol/kg	de Wit, Pierre
25	Alkalinity, total, standard deviation	AT std dev	±	de Wit, Pierre
26	Carbon, inorganic, dissolved	DIC	µmol/kg	de Wit, Pierre
27	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	de Wit, Pierre
28	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	de Wit, Pierre
29	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	de Wit, Pierre
30	Carbon, inorganic, dissolved	DIC	µmol/kg	de Wit, Pierre
31	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	de Wit, Pierre
32	Bicarbonate ion	[HCO3]-	µmol/kg	de Wit, Pierre
33	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	de Wit, Pierre
34	pH	pH		de Wit, Pierre		total scale
35	pH, standard deviation	pH std dev	±	de Wit, Pierre		total scale
36	Aragonite saturation state	Omega Arg		de Wit, Pierre
37	Aragonite saturation state, standard deviation	Omega Arg std dev	±	de Wit, Pierre
38	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
40	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

2422 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp	6 Treat	7 Time [h] (time post fertilization)	8 Ind [#] (count, replicate 1)	9 Ind [#] (partially calcified, replicate 1)	10 Ind [#] (fully calcified, replicate 1)	11 Ind [#] (count, replicate 2)	12 Ind [#] (partially calcified, replicate 2)	13 Ind [#] (fully calcified, replicate 2)	14 Calc I	15 Calc I std dev [±]	16 mRNA express	17 mRNA express std dev [±]	18 mRNA express std e [±]	19 mRNA express (Tyrosinase (contig CGI_100077...)	20 Temp [°C]	21 Temp std dev [±]	22 Sal	23 Sal std dev [±]	24 AT [µmol/kg]	25 AT std dev [±]	26 DIC [µmol/kg]	27 DIC std dev [±]	28 pCO2water_SST_wet [µatm]	29 pCO2 std dev [±]	30 DIC [µmol/kg]	31 DIC std dev [±]	32 [HCO3]- [µmol/kg]	33 [HCO3]- std dev [±]	34 pH (total scale)	35 pH std dev [±] (total scale)	36 Omega Arg	37 Omega Arg std dev [±]	38 CSC flag	39 pH (total scale)	40 CO2 [µmol/kg]	41 fCO2water_SST_wet [µatm]	42 pCO2water_SST_wet [µatm]	43 [HCO3]- [µmol/kg]	44 [CO3]2- [µmol/kg]	45 Omega Arg	46 Omega Cal
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	10	96	0	0	73	0	0	0.00000	0.00000					25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	12	70	0	0	66	0	0	0.00000	0.00000					25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	14	27	7	1	181	27	5	0.13444	0.04558					25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	16	159	27	78	105	39	31	0.52821	0.06684					25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	18	105	8	65	72	7	33	0.58204	0.10621					25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	10	69	0	0	117	0	0	0.00000	0.00000					25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	12	123	0	0	99	0	0	0.00000	0.00000					25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	14	56	0	0	46	0	0	0.00000	0.00000					25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	16	135	20	3	89	25	8	0.16332	0.09478					25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	18	65	4	43	82	17	23	0.53823	0.21790					25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	6	98	0	0	125	0	0	0.00000	0.00000					26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	10	119	12	0	54	2	0	0.03447	0.02256					26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	12	67	11	0	161	0	0	0.04104	0.05805					26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	14	90	27	26	80	26	53	0.63194	0.27302					26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	16	93	11	62	109	13	89	0.80098	0.10631					26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	18	79	6	70	64	3	60	0.94249	0.02608					26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	6	107	0	0	87	0	0	0.00000	0.00000					26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	10	55	5	0	178	7	0	0.03256	0.01824					26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	12	96	9	1	71	4	0	0.04273	0.02059					26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	14	95	25	0	175	91	0	0.19579	0.09081					26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	16	109	48	17	89	48	26	0.46897	0.13128					26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	18	94	14	72	308	20	240	0.82606	0.02032					26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	0									0.041379977	0.059723303	0.008053088		25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	6									0.055337560	0.083635689	0.011277434		25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	10									0.069074203	0.083530304	0.011263224		25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	12									0.093767772	0.108516609	0.014632377		25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	14									0.177920787	0.131681334	0.017755907		25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	16									0.444580762	0.159528604	0.021510833		25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	18									1.000000000	0.000000000	0.000000000		25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	0									0.041379977	0.059723303	0.008053088		25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	6									0.045575541	0.065236849	0.008796535		25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	10									0.062697492	0.072577580	0.009786359		25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	12									0.069892393	0.081228689	0.010952874		25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	14									0.117533260	0.104910539	0.014146134		25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	16									0.263756061	0.131122070	0.017680496		25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	18									0.603224967	0.132487818	0.017864654		25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	0												0.001711144	25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	6												0.007596699	25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	10												0.537466268	25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	12												0.724372795	25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	14												0.539957039	25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	16												0.247116033	25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	Control	18												0.176518859	25.8	0.49	30.4	0.00	2259	11	2053	13	565	65.6	1882	21	154.7	11.4	7.92	0.04	2.53	0.18	15	7.92	16.26	570.59	572	1880.1	156.61	2.56	3.92
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	0												0.001711144	25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	6												0.005642543	25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	10												0.461116830	25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	12												1.000000000	25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	14												0.686006986	25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	16												0.346275155	25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.1	High CO2	18												0.198408667	25.9	0.28	30.4	0.00	2285	14	2225	11	1515	104.1	2109	10	72.6	4.5	7.54	0.03	1.19	0.07	15	7.54	43.64	1535.27	1540	2107.9	73.47	1.20	1.84
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	0												0.004220373	26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	6												0.001994868	26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	10												0.611553502	26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	12												0.943372811	26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	14												0.246986523	26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	16												0.217387912	26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	Control	18												0.082357753	26.1	0.23	30.4	0.04	2251	18	2003	11	449	30.8	1808	15	182.4	10.6	8.01	0.03	2.99	0.17	15	8.00	12.88	455.33	457	1806.6	183.53	3.01	4.60
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	0												0.004220373	26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	6												0.003465501	26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	10												1.000000000	26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	12												0.703934861	26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	14												0.578031157	26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	16												0.221036894	26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	Exp.2	High CO2	18												0.097162137	26.3	0.28	30.4	0.12	2284	14	2194	13	1242	109.2	2071	14	87.5	6.8	7.62	0.04	1.44	0.11	15	7.63	35.12	1247.72	1252	2070.2	88.69	1.45	2.22