Citation:

s41396-018-0326-3

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Abstract:

A large fraction of the organic matter fixed in the oceans is transformed and remineralised by marine heterotrophic microorganisms. They, therefore, play a critical role in the marine carbon cycle.

In this study, we set out to identify the roles played by individual heterotrophic bacteria in the degradation of high molecular weight polysaccharides. At five sites in the Atlantic Ocean, we investigated the processing of organic matter in microbial communities by tracking the changes in community composition (fluorescence in situ hybridisation (FISH), 16S rRNA tag sequencing) in substrate incubation using a defined concentration of a known fluorescently labelled polysaccharide (FLA-laminarin, FLA-xylan, and FLA-chondroitin sulfate). Additionally, we tracked the dynamics of substrate processing (selfish uptake and extracellular hydrolysis) within the microbial communities between sites.

We found that the same substrate was processed in different ways by different members of a pelagic microbial community which points to significant follow-on effects for carbon cycling.

Coverage:

Median Latitude: 5.614180 * Median Longitude: -27.350300 * South-bound Latitude: -26.956000 * West-bound Longitude: -39.797700 * North-bound Latitude: 41.654200 * East-bound Longitude: -21.264900

Date/Time Start: 2012-10-15T13:03:00 * Date/Time End: 2012-11-09T13:59:00

Minimum DEPTH, water: 20 m * Maximum DEPTH, water: 20 m

Event(s):

JC079-04 (ATM22_JC079_CTD-8) * Latitude: 41.654200 * Longitude: -21.264900 * Date/Time: 2012-10-15T13:03:00 * Campaign: (ATM22) * Basis: * Method/Device: (CTD-RO) * Comment: PE Curves

JC079-12 (ATM22_JC079_CTD-24) * Latitude: 22.075000 * Longitude: -39.797700 * Date/Time: 2012-10-23T14:01:00 * Campaign: (ATM22) * Basis: * Method/Device: (CTD-RO) * Comment: PE Curves

JC079-19 (ATM22_JC079_CTD-38) * Latitude: 2.919700 * Longitude: -25.661700 * Date/Time: 2012-10-30T14:01:00 * Campaign: (ATM22) * Basis: * Method/Device: (CTD-RO) * Comment: PE Curves

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Reintjes, Greta
2	Optional event label	Event 2		Reintjes, Greta
3	Latitude of event	Latitude		Reintjes, Greta
4	Longitude of event	Longitude		Reintjes, Greta
5	Date/Time of event	Date/Time		Reintjes, Greta
6	Sample ID	Sample ID		Reintjes, Greta
7	DEPTH, water	Depth water	m	Reintjes, Greta		Geocode
8	Substrate type	Substrate		Reintjes, Greta
9	Experiment day	Exp day	day	Reintjes, Greta		Sampling timepoint
10	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
11	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)	Of substrate stained cells
12	Abundance	Abund	%	Reintjes, Greta	Fluorescence in situ hybridization (FISH)	Of substrate stained cells
13	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)	Average
14	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)	Of substrate stained cells
15	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)	CF319a filter piece
16	Bacteroidetes, targeted with CF319a oligonucliotide FISH-Probe	CF319a	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
17	Bacteroidetes, substrate stained	Bacteroidetes sub stain	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
18	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)	ALT1413 filter piece
19	Alteromonadales, targeted with ALT1413 oligonucleiotide FISH-Probe	ALT1413	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
20	Alteromonadales, substrate stained	Alteromonadales sub stain	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
21	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)	PLA46 filter piece
22	Planctomycetes, targeted with PLA46 oligonucliotide FISH-Probe	PLA46	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
23	Planctomycetes, substrate stained	Planctomycetes sub stain	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
24	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)	CAT653 filter piece
25	Catenovulum, targets with CAT653 oligonucleotide FISH-Probe	CAT653	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)
26	Catenovulum, substrate stained	Catenovulum sub stain	#/ml	Reintjes, Greta	Fluorescence in situ hybridization (FISH)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

3490 data points

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