Bienhold, Christina; Boetius, Antje (2018): Extracellular enzymatic activity from the Arctic Ocean during POLARSTERN cruise ARK-XXVII/3 (IceArc) from August-September 2012 [dataset]. Alfred Wegener Institute, Helmholtz Centre for Polar and Marine Research, Bremerhaven, PANGAEA, https://doi.org/10.1594/PANGAEA.892284
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Abstract:
Sampling was conducted with a TV-guided multiple corer (MUC), in order to retrieve undisturbed sediment cores. Upon retrieval of the MUC, the overlying water was carefully removed from each core and the core was cut into the following sediment horizons using a steel plate and a custom-made plastic ring: 0-1 cm, 1-5 cm, 5-10 cm. At each station, sediment samples from three replicate cores were pooled and transferred to a cooling container (0°C), where subsamples for extracellular enzymatic activity analyses were taken. The potential extracellular enzymatic activities of the hydrolases beta-glucosidase, chitobiase, aminopeptidase and extracellular enzymes acting as esterases were determined in alignment with Boetius and Lochte, 1994 (https://doi.org/10.3354/MEPS104299) and Boetius & Damm 1998 (https://doi.org/10.1016/S0967-0637(97)00052-6). The method uses small substrate proxies linked to a fluorophore. When the fluorophore is cleaved from the substrate, enzymatic hydrolysis is measured as an increase in fluorescence with time. Determinations of extracellular enzymatic activities were performed in triplicate for all samples with a Hitachi F-2000 spectrofluorometer.
Related to:
Bienhold, Christina (2021): Benthic bacterial diversity along the Laptev Sea continental slope in the Arctic Ocean between two contrasting years (1993 and 2012). insdc:PRJEB43433
Bienhold, Christina; Schourup-Kristensen, Vibe; Krumpen, Thomas; Nöthig, Eva-Maria; Wenzhöfer, Frank; Korhonen, Meri; Vredenborg, Myriel; Hehemann, Laura; Boetius, Antje (2022): Effects of sea ice retreat and ocean warming on the Laptev Sea continental slope ecosystem (1993 vs 2012). Frontiers in Marine Science, 9, 1004959, https://doi.org/10.3389/fmars.2022.1004959
Project(s):
Funding:
Seventh Framework Programme (FP7), grant/award no. 294757: Assessment of bacterial life and matter cycling in deep-sea surface sediments
Coverage:
Median Latitude: 78.147256 * Median Longitude: 124.209741 * South-bound Latitude: 77.169170 * West-bound Longitude: 114.916000 * North-bound Latitude: 79.649820 * East-bound Longitude: 136.967680
Date/Time Start: 2012-08-28T21:22:00 * Date/Time End: 2012-09-02T20:39:00
Minimum DEPTH, sediment/rock: 0.005 m * Maximum DEPTH, sediment/rock: 0.075 m
Event(s):
PS80/292-1 * Latitude: 79.649820 * Longitude: 130.597470 * Date/Time: 2012-08-28T21:22:00 * Elevation: -3387.4 m * Location: Arctic Ocean * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: Multicorer with television (TVMUC) * Comment: Position sensor: Ship's GPS
PS80/296-1 * Latitude: 78.388620 * Longitude: 133.201500 * Date/Time: 2012-08-29T16:32:00 * Elevation: -1986.4 m * Location: Laptev Sea * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: Multicorer with television (TVMUC) * Comment: 1963 m, GE 52.2, Bodensicht; Position sensor: USBL Ixsea Posidonia
PS80/299-1 * Latitude: 78.136150 * Longitude: 133.332780 * Date/Time: 2012-08-29T23:16:00 * Elevation: -786.6 m * Location: Laptev Sea * Campaign: ARK-XXVII/3 (PS80 IceArc) * Basis: Polarstern * Method/Device: Multicorer with television (TVMUC) * Comment: abgesetzt, SL=775m; Position sensor: USBL Ixsea Posidonia
Comment:
The substrates and final concentrations used in the assays were the following:
100 µM 4-Methylumbelliferyl ß-D-glucopyranoside to measure the activity of beta-glucosidases.
100 µM 4-Methylumbelliferyl N-acetyl-ß-D-glucosaminide to measure the activity of chitobiases .
500 µM L-Leucine-7-amino-4- methylcoumarin hydrochloride to measure the activity of aminopeptidases.
100 µM Fluorescein diacetate to measure the activity of esterases.
Parameter(s):
| # | Name | Short Name | Unit | Principal Investigator | Method/Device | Comment |
|---|---|---|---|---|---|---|
| 1 | Event label | Event | Bienhold, Christina | |||
| 2 | DEPTH, sediment/rock | Depth sed | m | Bienhold, Christina | Geocode | |
| 3 | beta-glucosidase activity | MUF-bglu | µmol/l/h | Bienhold, Christina | Fluorescence spectrometer (Hitachi F2000) | |
| 4 | Chitobiase activity | Chitobiase activity | µmol/l/h | Bienhold, Christina | Fluorescence spectrometer (Hitachi F2000) | |
| 5 | Aminopeptidase activity | MCA-Aminopept | µmol/l/h | Bienhold, Christina | Fluorescence spectrometer (Hitachi F2000) | |
| 6 | Esterase activity per sediment volume | FDA | nmol/ml/h | Bienhold, Christina | Fluorescence spectrometer (Hitachi F2000) | |
| 7 | Sample comment | Sample comment | Bienhold, Christina |
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Size:
147 data points
Data
| 1 Event | 2 Depth sed [m] | 3 MUF-bglu [µmol/l/h] | 4 Chitobiase activity [µmol/l/h] | 5 MCA-Aminopept [µmol/l/h] | 6 FDA [nmol/ml/h] | 7 Sample comment |
|---|---|---|---|---|---|---|
| PS80/292-1 | 0.005 | 0.18 | 0.40 | 160.49 | 8700 | corresponds to PS2455-1 from 1993 |
| PS80/292-1 | 0.030 | 0.03 | 0.03 | 133.32 | 5240 | corresponds to PS2455-1 from 1993 |
| PS80/292-1 | 0.075 | 0.01 | 0.01 | 241.17 | 10640 | corresponds to PS2455-1 from 1993 |
| PS80/296-1 | 0.005 | 0.22 | 0.68 | 83.60 | 4890 | corresponds to PS2457-1 from 1993 |
| PS80/296-1 | 0.030 | 0.09 | 0.30 | 91.90 | 4860 | corresponds to PS2457-1 from 1993 |
| PS80/296-1 | 0.075 | 0.00 | 0.00 | 39.90 | 380 | corresponds to PS2457-1 from 1993 |
| PS80/299-1 | 0.005 | 0.56 | 1.78 | 73.80 | 8640 | corresponds to PS2458-1 from 1993 |
| PS80/299-1 | 0.030 | 0.14 | 0.30 | 73.00 | 4560 | corresponds to PS2458-1 from 1993 |
| PS80/299-1 | 0.075 | 0.10 | 0.13 | 48.00 | 2320 | corresponds to PS2458-1 from 1993 |
| PS80/302-1 | 0.005 | 1.25 | 1.56 | 64.22 | 13990 | extra station shallow, similar depth as PS2462-1 from 1993 |
| PS80/302-1 | 0.030 | 0.42 | 1.78 | 55.11 | 10130 | extra station shallow, similar depth as PS2462-1 from 1993 |
| PS80/302-1 | 0.075 | 0.32 | 0.29 | 17.14 | 19550 | extra station shallow, similar depth as PS2462-1 from 1993 |
| PS80/309-1 | 0.005 | 1.34 | 2.18 | 64.77 | 12340 | close to PS2479-1 from 1993 |
| PS80/309-1 | 0.030 | 0.57 | 0.98 | 27.97 | 29440 | close to PS2479-1 from 1993 |
| PS80/309-1 | 0.075 | 0.23 | 0.15 | 1.72 | 30140 | close to PS2479-1 from 1993 |
| PS80/310-1 | 0.005 | 3.34 | 131.38 | 18270 | corresponds to PS2477-1 from 1993 | |
| PS80/310-1 | 0.030 | 1.16 | 105.44 | 13770 | corresponds to PS2477-1 from 1993 | |
| PS80/310-1 | 0.075 | 0.10 | 10.24 | 6310 | corresponds to PS2477-1 from 1993 | |
| PS80/312-1 | 0.005 | 0.79 | 2.25 | 67.55 | 14230 | corresponds to PS2476-1 from 1993 |
| PS80/312-1 | 0.030 | 0.18 | 0.32 | 54.26 | 6030 | corresponds to PS2476-1 from 1993 |
| PS80/312-1 | 0.075 | 0.27 | 0.27 | 56.24 | 4680 | corresponds to PS2476-1 from 1993 |
| PS80/313-1 | 0.005 | 0.29 | 0.91 | 62.96 | 6300 | corresponds to PS2474-1 from 1993 |
| PS80/313-1 | 0.030 | 0.04 | 0.14 | 92.44 | 5580 | corresponds to PS2474-1 from 1993 |
| PS80/313-1 | 0.075 | 0.06 | 0.14 | 79.66 | 3640 | corresponds to PS2474-1 from 1993 |
| PS80/318-1 | 0.005 | 0.21 | 0.56 | 143.86 | 14580 | corresponds to PS2472-1 from 1993 |
| PS80/318-1 | 0.030 | 0.02 | 0.06 | 297.89 | 16270 | corresponds to PS2472-1 from 1993 |
| PS80/318-1 | 0.075 | 0.00 | 0.01 | 267.93 | 14200 | corresponds to PS2472-1 from 1993 |
| PS80/320-1 | 0.005 | 0.15 | 0.33 | 180.91 | 14220 | corresponds to PS2471-1 from 1993 |
| PS80/320-1 | 0.030 | 0.01 | 0.01 | 320.60 | 16630 | corresponds to PS2471-1 from 1993 |
| PS80/320-1 | 0.075 | 0.00 | 0.00 | 218.78 | 12750 | corresponds to PS2471-1 from 1993 |