LeKieffre, Charlotte (2018): Inorganic carbon and nitrogen assimilation in cellular compartments of a benthic kleptoplastic foraminifer [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.891407,

---

Supplement to: LeKieffre, Charlotte; Jauffrais, Thierry; Geslin, Emmanuelle; Jesus, Bruno; Bernhard, Joan M; Giovani, Maria-Evangelia; Meibom, Anders (2018): Inorganic carbon and nitrogen assimilation in cellular compartments of a benthic kleptoplastic foraminifer. Scientific Reports, 8(1), https://doi.org/10.1038/s41598-018-28455-1

Haynesina germanica, an ubiquitous benthic foraminifer in intertidal mudflats, has the remarkable ability to isolate, sequester, and use chloroplasts from microalgae. The photosynthetic functionality of these kleptoplasts has been demonstrated by measuring photosystem II quantum efficiency and O2 production rates, but the precise role of the kleptoplasts in foraminiferal metabolism is poorly understood. Thus, the mechanism and dynamics of C and N assimilation and translocation from the kleptoplasts to the foraminiferal host requires study. The objective of this study was to investigate, using correlated TEM and NanoSIMS imaging, the assimilation of inorganic C and N (here ammonium, NH4+) in individuals of a kleptoplastic benthic foraminiferal species. H. germanica specimens were incubated for 20 h in artificial seawater enriched with H13CO3- and 15NH4+ during a light/dark cycle.

Data: The dataset contains one Excel Workbook (.xlsx).

The software Look@NanoSIMS was used to determine the isotopic enrichment of specific organelles that were identified morphologically from TEM images. Regions of interest (ROIs) to quantify the average isotopic enrichment of the organelles were defined from the TEM images previously aligned with the NanoSIMS images (based on the 12C14N image). For the average isotopic enrichment of the cytoplasm, three circles of ca. 2 µm in diameter were drawn per image, avoiding highly 15N-enriched organelles. For each specimen, between one and three NanoSIMS images were analyzed.