Citation:

j.scitotenv.2018.01.056

Name: Seawater carbonate chemistry and immune function of the Pacific oyster against Vibrio splendidus challenge
Published: 2018-05-02
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Immunology/Self-protection; Laboratory experiment; Mollusca; North Pacific; Other; Single species; Temperate

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Abstract:

Ocean acidification (OA) and pathogenic diseases pose a considerable threat to key species of marine ecosystem. However, few studies have investigated the combined impact of reduced seawater pH and pathogen challenge on the immune responses of marine invertebrates. In this study, Pacific oysters, Crassostrea gigas, were exposed to OA (~2000 ppm) for 28 days and then challenged with Vibrio splendidus for another 72 h. Hemocyte parameters showed that V. splendidus infection exacerbated the impaired oyster immune responses under OA exposure. An iTRAQ-based quantitative proteomic analysis revealed that C. gigas responded differently to OA stress and V. splendidus challenge, alone or in combination. Generally, OA appears to act via a generalized stress response by causing oxidative stress, which could lead to cellular injury and cause disruption to the cytoskeleton, protein turnover, immune responses and energy metabolism. V. splendidus challenge in oysters could suppress the immune system directly and lead to a disturbed cytoskeleton structure, increased protein turnover and energy metabolism suppression, without causing oxidative stress. The combined OA- and V. splendidus-treated oysters ultimately presented a similar, but stronger proteomic response pattern compared with OA treatment alone. Overall, the impaired oyster immune functions caused by OA exposure may have increased the risk of V. splendidus infection. These results have important implications for the impact of OA on disease outbreaks in marine invertebrates, which would have significant economic and ecological repercussions.

Keyword(s):

Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Crassostrea gigas; Immunology/Self-protection; Laboratory experiment; Mollusca; North Pacific; Other; Single species; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-05-02.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Zhao, Jianmin		study
2	Species	Species		Zhao, Jianmin
3	Registration number of species	Reg spec no		Zhao, Jianmin
4	Uniform resource locator/link to reference	URL ref		Zhao, Jianmin		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Zhao, Jianmin
6	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Zhao, Jianmin		treatment
7	Treatment	Treat		Zhao, Jianmin
8	Phagocytosis rate	Phagocytosis rate	%	Zhao, Jianmin
9	Phagocytosis rate, standard deviation	Phago std dev	±	Zhao, Jianmin
10	Reactive oxygen species production	ROS prod		Zhao, Jianmin
11	Reactive oxygen species production, standard deviation	ROS prod std dev	±	Zhao, Jianmin
12	Percentage	Perc	%	Zhao, Jianmin		dead hemocytes
13	Percentage, standard deviation	Perc std dev	±	Zhao, Jianmin		dead hemocytes
14	Hemocyte count	Hemocyte	#	Zhao, Jianmin
15	Hemocyte count, standard deviation	Hemocyte std dev	±	Zhao, Jianmin
16	Number of expressed proteins	Express prot	#	Zhao, Jianmin		up-regulated
17	Number of expressed proteins	Express prot	#	Zhao, Jianmin		down-regulated
18	Name	Name		Zhao, Jianmin
19	Group	Group		Zhao, Jianmin
20	Fold change	Fold change		Zhao, Jianmin		Log2 Fold change
21	Gene expression	Gene expression		Zhao, Jianmin
22	Gene expression, standard deviation	Gene expression std dev	±	Zhao, Jianmin
23	Temperature, water	Temp	°C	Zhao, Jianmin
24	Temperature, water, standard deviation	Temp std dev	±	Zhao, Jianmin
25	Salinity	Sal		Zhao, Jianmin
26	Salinity, standard deviation	Sal std dev	±	Zhao, Jianmin
27	pH	pH		Zhao, Jianmin	Potentiometric	NBS scale
28	pH, standard deviation	pH std dev	±	Zhao, Jianmin	Potentiometric	NBS scale
29	Alkalinity, total	AT	µmol/kg	Zhao, Jianmin	Potentiometric titration
30	Alkalinity, total, standard deviation	AT std dev	±	Zhao, Jianmin	Potentiometric titration
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Zhao, Jianmin	Calculated using CO2SYS
32	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Zhao, Jianmin	Calculated using CO2SYS
33	Carbon, inorganic, dissolved	DIC	µmol/kg	Zhao, Jianmin	Calculated using CO2SYS
34	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Zhao, Jianmin	Calculated using CO2SYS
35	Calcite saturation state	Omega Cal		Zhao, Jianmin	Calculated using CO2SYS
36	Calcite saturation state, standard deviation	Omega Cal std dev	±	Zhao, Jianmin	Calculated using CO2SYS
37	Aragonite saturation state	Omega Arg		Zhao, Jianmin	Calculated using CO2SYS
38	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Zhao, Jianmin	Calculated using CO2SYS
39	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
41	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1458 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 pCO2water_SST_wet [ppmv] (treatment)	7 Treat	8 Phagocytosis rate [%]	9 Phago std dev [±]	10 ROS prod	11 ROS prod std dev [±]	12 Perc [%] (dead hemocytes)	13 Perc std dev [±] (dead hemocytes)	14 Hemocyte [#]	15 Hemocyte std dev [±]	16 Express prot [#] (up-regulated)	17 Express prot [#] (down-regulated)	18 Name	19 Group	20 Fold change (Log2 Fold change)	21 Gene expression	22 Gene expression std dev [±]	23 Temp [°C]	24 Temp std dev [±]	25 Sal	26 Sal std dev [±]	27 pH (NBS scale, Potentiometric)	28 pH std dev [±] (NBS scale, Potentiometric)	29 AT [µmol/kg] (Potentiometric titration)	30 AT std dev [±] (Potentiometric titration)	31 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	32 pCO2 std dev [±] (Calculated using CO2SYS)	33 DIC [µmol/kg] (Calculated using CO2SYS)	34 DIC std dev [±] (Calculated using CO2SYS)	35 Omega Cal (Calculated using CO2SYS)	36 Omega Cal std dev [±] (Calculated using CO2SYS)	37 Omega Arg (Calculated using CO2SYS)	38 Omega Arg std dev [±] (Calculated using CO2SYS)	39 CSC flag (Calculated using seacarb afte...)	40 pH (total scale, Calculated using...)	41 CO2 [µmol/kg] (Calculated using seacarb afte...)	42 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	43 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	44 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	45 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	46 DIC [µmol/kg] (Calculated using seacarb afte...)	47 Omega Arg (Calculated using seacarb afte...)	48 Omega Cal (Calculated using seacarb afte...)
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	non-inj	33.480	2.54	11.11	2.18	3.27	1.36	1110003	202103								13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	fssw-inj	31.380	2.92	7.26	2.48	3.60	1.22	1241440	398688								13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	vibrio-inj	28.900	5.80	5.88	1.40	7.96	1.81	1475970	192501								13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	non-inj	30.440	5.70	15.36	4.71	6.92	1.74	1028489	167150								12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	fssw-inj	27.325	4.09	14.69	2.43	7.52	1.31	1134528	152767								12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	vibrio-inj	18.950	2.99	10.74	3.65	10.38	2.85	1410377	182564								12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Vibrio									47	44
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA									44	44
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Vibrio-OA									94	89
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											calmodulin	gene	0.999
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											calmodulin	protein	0.490
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											defh2	gene	-3.344
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											defh2	protein	-2.519
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											integrin alpha-4	gene	0.653
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											integrin alpha-4	protein	0.654
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											Caspase-3	gene	3.013
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											Caspase-3	protein	0.192
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											GST	gene	1.078
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											GST	protein	-1.147
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											SOD	gene	1.618
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA											SOD	protein	0.132
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											calmodulin	gene	0.435
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											calmodulin	protein	1.146
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											defh2	gene	-5.227
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											defh2	protein	-2.837
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											integrin alpha-4	gene	0.062
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											integrin alpha-4	protein	0.884
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											Caspase-3	gene	-0.287
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											Caspase-3	protein	-0.148
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											GST	gene	-0.649
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											GST	protein	-0.160
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											SOD	gene	-0.969
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		Jun											SOD	protein	-0.654
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											calmodulin	gene	0.614
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											calmodulin	protein	-0.031
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											defh2	gene	-7.039
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											defh2	protein	-3.019
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											integrin alpha-4	gene	0.281
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											integrin alpha-4	protein	1.091
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											Caspase-3	gene	1.961
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											Caspase-3	protein	-1.120
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											GST	gene	1.261
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											GST	protein	-2.408
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											SOD	gene	0.264
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28		OA&jun											SOD	protein	1.063
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	fssw-inj											calmudulin			1.025	0.2772	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	fssw-inj											calmudulin			2.027	0.9760	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	fssw-inj											casp 3			1.103	0.6376	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	fssw-inj											casp 3			10.807	2.4511	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	fssw-inj											Defensin			1.163	0.3116	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	fssw-inj											Defensin			0.147	0.0986	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	fssw-inj											GST			1.139	0.6686	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	fssw-inj											GST			2.405	0.4886	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	fssw-inj											integrin			1.185	0.3036	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	fssw-inj											integrin			1.492	0.8889	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	fssw-inj											SOD			1.103	0.6376	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	fssw-inj											SOD			4.176	2.3278	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	vibrio-inj											calmudulin			1.418	0.6627	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	vibrio-inj											calmudulin			1.552	0.5620	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	vibrio-inj											casp 3			0.904	0.6126	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	vibrio-inj											casp 3			7.205	3.2144	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	vibrio-inj											Defensin			0.040	0.0088	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	vibrio-inj											Defensin			0.011	0.0097	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	vibrio-inj											GST			0.533	0.3100	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	vibrio-inj											GST			2.730	0.1592	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	vibrio-inj											integrin			1.078	0.4112	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	vibrio-inj											integrin			1.320	0.4243	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	400	vibrio-inj											SOD			0.232	0.0683	13.2	0.5	31.2	0.5	8.11	0.02	2317.13	45.18	482.39	9.65	2155.70	43.07	3.05	0.06	1.93	0.04	26	7.99	19.17	473.89	475.65	2010.06	125.52	2154.75	1.94	3.06
laboratory	Crassostrea gigas (mollusk)	140656	marinespecies.org	28	2000	vibrio-inj											SOD			1.542	0.7827	12.7	0.7	31.2	0.5	7.57	0.05	2349.39	55.19	1862.41	44.08	2368.28	56.06	0.97	0.02	0.61	0.01	26	7.45	75.27	1831.69	1838.54	2252.02	39.84	2367.13	0.62	0.97