Citation:

j.fsi.2017.10.034

Name: Seawater carbonate chemistry and microbial community composition and host-pathogen interactions between the blood clam and Vibrio harveyi
Published: 2017
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Bacteria; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Coast and continental shelf; Community composition and diversity; Containers and aquaria (20-1000 L or < 1 m**2); Entire community; Heterotrophic prokaryotes; Laboratory experiment; Mollusca; Mortality/Survival; North Pacific; Pelagos; Proteobacteria; Species interaction; Temperate; Vibrio harveyi

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Abstract:

It has been suggested that climate change may promote the outbreaks of diseases in the sea through altering the host susceptibility, the pathogen virulence, and the host-pathogen interaction. However, the impacts of ocean acidification (OA) on the pathogen components of bacterial community and the host-pathogen interaction of marine bivalves are still poorly understood. Therefore, 16S rRNA high-throughput sequencing and host-pathogen interaction analysis between blood clam (Tegillarca granosa) and Vibrio harveyi were conducted in the present study to gain a better understanding of the ecological impacts of ocean acidification. The results obtained revealed a significant impact of ocean acidification on the composition of microbial community at laboratory scale. Notably, the abundance of Vibrio, a major group of pathogens to many marine organisms, was significantly increased under ocean acidification condition. In addition, the survival rate and haemolytic activity of V. harveyi were significantly higher in the presence of haemolymph of OA treated T. granosa, indicating a compromised immunity of the clam and enhanced virulence of V. harveyi under future ocean acidification scenarios. Conclusively, the results obtained in this study suggest that future ocean acidification may increase the risk of Vibrio pathogen infection for marine bivalve species, such as blood clams.

Keyword(s):

Animalia; Bacteria; Benthic animals; Benthos; Biomass/Abundance/Elemental composition; Coast and continental shelf; Community composition and diversity; Containers and aquaria (20-1000 L or < 1 m**2); Entire community; Heterotrophic prokaryotes; Laboratory experiment; Mollusca; Mortality/Survival; North Pacific; Pelagos; Proteobacteria; Species interaction; Temperate; Vibrio harveyi

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-04-09.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Guangxu		study
2	Species	Species		Liu, Guangxu
3	Registration number of species	Reg spec no		Liu, Guangxu
4	Uniform resource locator/link to reference	URL ref		Liu, Guangxu		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Liu, Guangxu
6	Figure	Fig		Liu, Guangxu
7	Treatment	Treat		Liu, Guangxu
8	Replicate	Repl		Liu, Guangxu
9	Group	Group		Liu, Guangxu
10	Abundance	Abund		Liu, Guangxu		relative
11	Bacteria, abundance in colony forming units	Bacteria CFU	CFU/ml	Liu, Guangxu		yellow
12	Survival	Survival	%	Liu, Guangxu
13	pH	pH		Liu, Guangxu	Potentiometric	NBS scale
14	pH, standard error	pH std e	±	Liu, Guangxu	Potentiometric	NBS scale
15	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Liu, Guangxu	Calculated using CO2SYS
16	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Liu, Guangxu	Calculated using CO2SYS
17	Salinity	Sal		Liu, Guangxu
18	Salinity, standard error	Sal std e	±	Liu, Guangxu
19	Temperature, water	Temp	°C	Liu, Guangxu
20	Temperature, water, standard error	T std e	±	Liu, Guangxu
21	Alkalinity, total	AT	µmol/kg	Liu, Guangxu	Potentiometric titration
22	Alkalinity, total, standard error	AT std e	±	Liu, Guangxu	Potentiometric titration
23	Aragonite saturation state	Omega Arg		Liu, Guangxu	Calculated using CO2SYS
24	Aragonite saturation state, standard error	Omega Arg std e	±	Liu, Guangxu	Calculated using CO2SYS
25	Calcite saturation state	Omega Cal		Liu, Guangxu	Calculated using CO2SYS
26	Calcite saturation state, standard error	Omega Cal std e	±	Liu, Guangxu	Calculated using CO2SYS
27	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
28	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
29	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
31	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

7517 data points

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