Citation:

j.marenvres.2017.07.016

Name: Seawater carbonate chemistry and sperm-egg collisions, gamete fusion, and generation of Ca2+ oscillations of a broadcast spawning bivalve, Tegillarca granosa
Published: 2017
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Pacific; Other metabolic rates; Reproduction; Single species; Tegillarca granosa; Temperate

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Abstract:

Although the effect of ocean acidification on fertilization success of marine organisms is increasingly well documented, the underlying mechanisms are not completely understood. The fertilization success of broadcast spawning invertebrates depends on successful sperm-egg collisions, gamete fusion, and standard generation of Ca2+oscillations. Therefore, the realistic effects of future ocean pCO2 levels on these specific aspects of fertilization of Tegillarca granosa were investigated in the present study through sperm velocity trials, fertilization kinetics model analysis, and intracellular Ca2+assays, respectively. Results obtained indicated that ocean acidification significantly reduced the fertilization success of T. granosa, which could be accountable by (i) decreased sperm velocity hence reducing the probability for sperm-egg collisions; (ii) lowered probability of gamete fusion for each gamete collision event; and (iii) disrupted intracellular Ca2+ oscillations.

Keyword(s):

Animalia; Benthic animals; Benthos; Bottles or small containers/Aquaria (<20 L); Coast and continental shelf; Laboratory experiment; Mollusca; North Pacific; Other metabolic rates; Reproduction; Single species; Tegillarca granosa; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: 28.466670 * Longitude: 121.183330

Date/Time Start: 2015-06-01T00:00:00 * Date/Time End: 2015-06-30T00:00:00

Event(s):

Yueqing_Bay * Latitude: 28.466670 * Longitude: 121.183330 * Date/Time Start: 2015-06-01T00:00:00 * Date/Time End: 2015-06-30T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-04-09.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Guangxu		study
2	Species	Species		Liu, Guangxu
3	Registration number of species	Reg spec no		Liu, Guangxu
4	Uniform resource locator/link to reference	URL ref		Liu, Guangxu		WoRMS Aphia ID
5	Treatment	Treat		Liu, Guangxu
6	Replicate	Repl		Liu, Guangxu
7	Fertilization success rate	Fert success	%	Liu, Guangxu
8	pH	pH		Liu, Guangxu	Potentiometric	NBS scale
9	pH, standard error	pH std e	±	Liu, Guangxu	Potentiometric	NBS scale
10	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Liu, Guangxu	Calculated using CO2SYS
11	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), standard error	pCO2water_SST_wet std e	±	Liu, Guangxu	Calculated using CO2SYS
12	Salinity	Sal		Liu, Guangxu
13	Salinity, standard error	Sal std e	±	Liu, Guangxu
14	Temperature, water	Temp	°C	Liu, Guangxu
15	Temperature, water, standard error	T std e	±	Liu, Guangxu
16	Alkalinity, total	AT	µmol/kg	Liu, Guangxu	Potentiometric titration
17	Alkalinity, total, standard error	AT std e	±	Liu, Guangxu	Potentiometric titration
18	Carbon, inorganic, dissolved	DIC	µmol/kg	Liu, Guangxu	Calculated using CO2SYS
19	Carbon, inorganic, dissolved, standard error	DIC std e	±	Liu, Guangxu	Calculated using CO2SYS
20	Aragonite saturation state	Omega Arg		Liu, Guangxu	Calculated using CO2SYS
21	Aragonite saturation state, standard error	Omega Arg std e	±	Liu, Guangxu	Calculated using CO2SYS
22	Calcite saturation state	Omega Cal		Liu, Guangxu	Calculated using CO2SYS
23	Calcite saturation state, standard error	Omega Cal std e	±	Liu, Guangxu	Calculated using CO2SYS
24	Fertilization success rate	Fert success	%	Liu, Guangxu		sperm velocity induced
25	Probability	Prob		Liu, Guangxu		gamete fusion per collision
26	Fluorescence intensity	Fluorescence		Liu, Guangxu		Intracellular Ca2+
27	Amplitude	Amplitude		Liu, Guangxu
28	Curvilinear velocity	VCL	µm/s	Liu, Guangxu
29	Curvilinear velocity, standard error	VCL std e	±	Liu, Guangxu
30	Average path velocity	VAP	µm/s	Liu, Guangxu
31	Average path velocity, standard error	VAP std e	±	Liu, Guangxu
32	Straight line velocity	VSL	µm/s	Liu, Guangxu
33	Straight line velocity, standard error	VSL std e	±	Liu, Guangxu
34	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
36	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
41	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
42	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1080 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Treat	6 Repl	7 Fert success [%]	8 pH (NBS scale, Potentiometric)	9 pH std e [±] (NBS scale, Potentiometric)	10 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	11 pCO2water_SST_wet std e [±] (Calculated using CO2SYS)	12 Sal	13 Sal std e [±]	14 Temp [°C]	15 T std e [±]	16 AT [µmol/kg] (Potentiometric titration)	17 AT std e [±] (Potentiometric titration)	18 DIC [µmol/kg] (Calculated using CO2SYS)	19 DIC std e [±] (Calculated using CO2SYS)	20 Omega Arg (Calculated using CO2SYS)	21 Omega Arg std e [±] (Calculated using CO2SYS)	22 Omega Cal (Calculated using CO2SYS)	23 Omega Cal std e [±] (Calculated using CO2SYS)	24 Fert success [%] (sperm velocity induced)	25 Prob (gamete fusion per collision)	26 Fluorescence (Intracellular Ca2+)	27 Amplitude	28 VCL [µm/s]	29 VCL std e [±]	30 VAP [µm/s]	31 VAP std e [±]	32 VSL [µm/s]	33 VSL std e [±]	34 CSC flag (Calculated using seacarb afte...)	35 pH (total scale, Calculated using...)	36 CO2 [µmol/kg] (Calculated using seacarb afte...)	37 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	38 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	39 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	40 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	41 DIC [µmol/kg] (Calculated using seacarb afte...)	42 Omega Arg (Calculated using seacarb afte...)
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	1	0.89	8.11	0.09	589.35	10.15	21.13	0.05	25.28	0.12	2068.83	12.29	1898.93	2.76	2.17	0.11	3.43	0.03	0.62	0.16	3333601.00	263511.51							26	7.96	16.06	530.10	531.79	1779.91	122.82	1918.79	2.06
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	2	0.70	8.11	0.10	589.35	10.16	21.13	0.06	25.28	0.13	2068.83	12.30	1898.93	2.77	2.17	0.12	3.43	0.04	0.63	0.16	3602085.00	287366.41							26	7.96	16.06	530.10	531.79	1779.91	122.82	1918.79	2.06
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	3	0.90	8.11	0.11	589.35	10.17	21.13	0.07	25.28	0.14	2068.83	12.31	1898.93	2.78	2.17	0.13	3.43	0.05	0.71	0.26	3468949.00	217059.11							26	7.96	16.06	530.10	531.79	1779.91	122.82	1918.79	2.06
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	4	0.94	8.11	0.12	589.35	10.18	21.13	0.08	25.28	0.15	2068.83	12.32	1898.93	2.79	2.17	0.14	3.43	0.06	0.57	0.16	3622007.00	255799.98							26	7.96	16.06	530.10	531.79	1779.91	122.82	1918.79	2.06
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	5	0.92	8.11	0.13	589.35	10.19	21.13	0.09	25.28	0.16	2068.83	12.33	1898.93	2.80	2.17	0.15	3.43	0.07	0.72	0.19	3558669.00	217321.65							26	7.96	16.06	530.10	531.79	1779.91	122.82	1918.79	2.06
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	6	0.95	8.11	0.14	589.35	10.20	21.13	0.10	25.28	0.17	2068.83	12.34	1898.93	2.81	2.17	0.16	3.43	0.08	0.73	0.20	3551389.00	254686.94							26	7.96	16.06	530.10	531.79	1779.91	122.82	1918.79	2.06
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	7	0.74	8.11	0.15	589.35	10.21	21.13	0.11	25.28	0.18	2068.83	12.35	1898.93	2.82	2.17	0.17	3.43	0.09	0.66	0.21	3662724.00	244229.74							26	7.96	16.06	530.10	531.79	1779.91	122.82	1918.79	2.06
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	8	0.81	8.11	0.16	589.35	10.22	21.13	0.12	25.28	0.19	2068.83	12.36	1898.93	2.83	2.17	0.18	3.43	0.10	0.65	0.24	3533561.00	192303.11							26	7.96	16.06	530.10	531.79	1779.91	122.82	1918.79	2.06
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1	9	0.79	8.11	0.17	589.35	10.23	21.13	0.13	25.28	0.20	2068.83	12.37	1898.93	2.84	2.17	0.19	3.43	0.11	0.67	0.24	3626451.00	247172.10							26	7.96	16.06	530.10	531.79	1779.91	122.82	1918.79	2.06
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	1	0.82	7.78	0.11	1347.24	9.41	21.21	0.09	25.42	0.23	2071.49	9.26	1984.46	0.59	1.11	0.09	1.76	0.07	0.61	0.14	3542728.00	269900.86							26	7.63	36.99	1225.83	1229.74	1924.32	62.55	2023.86	1.05
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	2	0.66	7.78	0.11	1347.24	9.41	21.21	0.09	25.42	0.23	2071.49	9.26	1984.46	0.59	1.11	0.09	1.76	0.07	0.67	0.16	3568758.00	233905.25							26	7.63	36.99	1225.83	1229.74	1924.32	62.55	2023.86	1.05
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	3	0.76	7.78	0.11	1347.24	9.41	21.21	0.09	25.42	0.23	2071.49	9.26	1984.46	0.59	1.11	0.09	1.76	0.07	0.63	0.18	3677271.00	235090.86							26	7.63	36.99	1225.83	1229.74	1924.32	62.55	2023.86	1.05
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	4	0.78	7.78	0.11	1347.24	9.41	21.21	0.09	25.42	0.23	2071.49	9.26	1984.46	0.59	1.11	0.09	1.76	0.07	0.55	0.22	3702192.00	245466.41							26	7.63	36.99	1225.83	1229.74	1924.32	62.55	2023.86	1.05
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	5	0.83	7.78	0.11	1347.24	9.41	21.21	0.09	25.42	0.23	2071.49	9.26	1984.46	0.59	1.11	0.09	1.76	0.07	0.61	0.22	3749179.00	246638.94							26	7.63	36.99	1225.83	1229.74	1924.32	62.55	2023.86	1.05
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	6	0.75	7.78	0.11	1347.24	9.41	21.21	0.09	25.42	0.23	2071.49	9.26	1984.46	0.59	1.11	0.09	1.76	0.07	0.63	0.13	3297991.00	222900.30							26	7.63	36.99	1225.83	1229.74	1924.32	62.55	2023.86	1.05
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	7	0.71	7.78	0.11	1347.24	9.41	21.21	0.09	25.42	0.23	2071.49	9.26	1984.46	0.59	1.11	0.09	1.76	0.07	0.51	0.08	3153258.00	237261.58							26	7.63	36.99	1225.83	1229.74	1924.32	62.55	2023.86	1.05
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	8	0.64	7.78	0.11	1347.24	9.41	21.21	0.09	25.42	0.23	2071.49	9.26	1984.46	0.59	1.11	0.09	1.76	0.07	0.66	0.14	3463122.00	271324.16							26	7.63	36.99	1225.83	1229.74	1924.32	62.55	2023.86	1.05
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8	9	0.70	7.78	0.11	1347.24	9.41	21.21	0.09	25.42	0.23	2071.49	9.26	1984.46	0.59	1.11	0.09	1.76	0.07	0.59	0.19	3405355.00	255984.69							26	7.63	36.99	1225.83	1229.74	1924.32	62.55	2023.86	1.05
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	1	0.53	7.38	0.08	3252.44	15.58	21.19	0.13	25.13	0.17	2059.59	13.28	2020.74	1.79	0.50	0.04	0.79	0.13	0.50	0.09	3096629.00	152552.84							26	7.23	96.98	3189.56	3199.79	1999.31	25.62	2121.91	0.43
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	2	0.52	7.38	0.08	3252.44	15.58	21.19	0.13	25.13	0.17	2059.59	13.28	2020.74	1.79	0.50	0.04	0.79	0.13	0.51	0.10	3230446.00	158811.53							26	7.23	96.98	3189.56	3199.79	1999.31	25.62	2121.91	0.43
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	3	0.57	7.38	0.08	3252.44	15.58	21.19	0.13	25.13	0.17	2059.59	13.28	2020.74	1.79	0.50	0.04	0.79	0.13	0.49	0.07	3045967.00	165602.75							26	7.23	96.98	3189.56	3199.79	1999.31	25.62	2121.91	0.43
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	4	0.50	7.38	0.08	3252.44	15.58	21.19	0.13	25.13	0.17	2059.59	13.28	2020.74	1.79	0.50	0.04	0.79	0.13	0.53	0.03	3219684.00	180819.06							26	7.23	96.98	3189.56	3199.79	1999.31	25.62	2121.91	0.43
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	5	0.60	7.38	0.08	3252.44	15.58	21.19	0.13	25.13	0.17	2059.59	13.28	2020.74	1.79	0.50	0.04	0.79	0.13	0.52	0.07	3034646.00	170389.67							26	7.23	96.98	3189.56	3199.79	1999.31	25.62	2121.91	0.43
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	6	0.64	7.38	0.08	3252.44	15.58	21.19	0.13	25.13	0.17	2059.59	13.28	2020.74	1.79	0.50	0.04	0.79	0.13	0.52	0.13	1974991.00	167016.45							26	7.23	96.98	3189.56	3199.79	1999.31	25.62	2121.91	0.43
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	7	0.73	7.38	0.08	3252.44	15.58	21.19	0.13	25.13	0.17	2059.59	13.28	2020.74	1.79	0.50	0.04	0.79	0.13	0.50	0.11	3360093.00	208461.78							26	7.23	96.98	3189.56	3199.79	1999.31	25.62	2121.91	0.43
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	8	0.54	7.38	0.08	3252.44	15.58	21.19	0.13	25.13	0.17	2059.59	13.28	2020.74	1.79	0.50	0.04	0.79	0.13	0.50	0.12	3045821.00	181305.80							26	7.23	96.98	3189.56	3199.79	1999.31	25.62	2121.91	0.43
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4	9	0.46	7.38	0.08	3252.44	15.58	21.19	0.13	25.13	0.17	2059.59	13.28	2020.74	1.79	0.50	0.04	0.79	0.13	0.51	0.08	3781210.00	202501.31							26	7.23	96.98	3189.56	3199.79	1999.31	25.62	2121.91	0.43
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 8.1			8.11	0.17	589.35	10.23	21.13	0.13	25.28	0.20	2068.83	12.37	1898.93	2.84	2.17	0.19	3.43	0.11					113.78	4.58	114.19	5.16	83.41	4.69	26	7.96	16.06	530.10	531.79	1779.91	122.82	1918.79	2.06
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.8			7.78	0.11	1347.24	9.41	21.21	0.09	25.42	0.23	2071.49	9.26	1984.46	0.59	1.11	0.09	1.76	0.07					99.54	3.98	100.54	4.49	72.67	4.02	26	7.63	36.99	1225.83	1229.74	1924.32	62.55	2023.86	1.05
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	pH 7.4			7.38	0.08	3252.44	15.58	21.19	0.13	25.13	0.17	2059.59	13.28	2020.74	1.79	0.50	0.04	0.79	0.13					83.82	3.32	78.74	2.92	50.81	5.62	26	7.23	96.98	3189.56	3199.79	1999.31	25.62	2121.91	0.43