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Nardi, Alessandro; Benedetti, Maura; Fattorini, Daniele; Regoli, Francesco (2018): Seawater carbonate chemistry and cadmium uptake of the smooth scallop Flexopecten glaber [dataset]. PANGAEA, https://doi.org/10.1594/PANGAEA.887768, Supplement to: Nardi, A et al. (2018): Oxidative and interactive challenge of cadmium and ocean acidification on the smooth scallop Flexopecten glaber. Aquatic Toxicology, 196, 53-60, https://doi.org/10.1016/j.aquatox.2018.01.008

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Abstract:
Ocean acidification (OA) may affect sensitivity of marine organisms to metal pollution modulating chemical bioavailability, bioaccumulation and biological responsiveness of several cellular pathways. In this study, the smooth scallop Flexopecten glaber was exposed to various combinations of reduced pH (pH/pCO2 7.4/3000 µatm) and Cd (20 µg/L). The analyses on cadmium uptake were integrated with those of a wide battery of biomarkers including metallothioneins, single antioxidant defenses and total oxyradical scavenging capacity in digestive gland and gills, lysosomal membrane stability and onset of genotoxic damage in haemocytes. Reduced pH slightly increased concentration of Cd in scallop tissues, but no effects were measured in terms of metallothioneins. Induction of some antioxidants by Cd and/or low pH in the digestive gland was not reflected in variations of the total oxyradical scavenging capacity, while the investigated stressors caused a certain inhibition of antioxidants and reduction of the scavenging capacity toward peroxyl radical in the gills. Lysosomal membrane stability and onset of genotoxic damages showed high sensitivity with possible synergistic effects of the investigated factors. The overall results suggest that indirect effects of ocean acidification on metal accumulation and toxicity are tissue-specific and modulate oxidative balance through different mechanisms.
Keyword(s):
Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Flexopecten glaber; Immunology/Self-protection; Inorganic toxins; Laboratory experiment; Mediterranean Sea; Mollusca; Single species; Temperate
Further details:
Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb
Project(s):
Ocean Acidification International Coordination Centre (OA-ICC)
Comment:
In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-03-28.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1 TypeTypeRegoli, Francescostudy
2 SpeciesSpeciesRegoli, Francesco
3 Registration number of speciesReg spec noRegoli, Francesco
4 Uniform resource locator/link to referenceURL refRegoli, FrancescoWoRMS Aphia ID
5 TreatmentTreatRegoli, Francesco
6 IdentificationIDRegoli, Francescolabel
7 Experiment durationExp durationdaysRegoli, Francesco
8 Temperature, waterTemp°CRegoli, Francesco
9 Salinity, standard deviationSal std dev±Regoli, Francesco
10 CadmiumCdµg/gRegoli, Francescoin digestive gland, per dry mass
11 Cadmium, standard deviationCd std dev±Regoli, Francescoin digestive gland, per dry mass
12 Metallothionein, per protein massMT/protnmol/mgRegoli, Francescoin digestive gland
13 Metallothionein per protein, standard deviationMT std dev±Regoli, Francescoin digestive gland
14 Glutathione, total, per unit wet massTGSHµmol/gRegoli, Francescoin digestive gland
15 Glutathione, total, per unit wet mass, standard deviationTGSH std dev±Regoli, Francescoin digestive gland
16 Catalase activity, per protein massCAT/protµmol/min/mgRegoli, Francescoin digestive gland
17 Catalase activity, standard deviationCAT std dev±Regoli, Francescoin digestive gland
18 Glutathione S-transferase, activity per protein massGST/protnmol/min/mgRegoli, Francescoin digestive gland
19 Glutathione S-transferase, activity per protein mass, standard deviationGST std dev±Regoli, Francescoin digestive gland
20 Glutathione reductase, per protein massGR/protnmol/min/mgRegoli, Francescoin digestive gland
21 Glutathione reductase, unit per protein mass, standard deviationGR std dev±Regoli, Francescoin digestive gland
22 Selenium dependent glutathione peroxidases activity, per protein massGPxSe-Dep/protnmol/min/mgRegoli, Francescoin digestive gland
23 Selenium dependent glutathione peroxidases activity, unit per protein mass, standard deviationGPxSe-Dep std dev±Regoli, Francescoin digestive gland
24 Total glutathione peroxidases activity, per protein massGPxTOT/protnmol/min/mgRegoli, Francescoin digestive gland
25 Total glutathione peroxidases activity, unit per protein mass, standard deviationGPxTOT std dev±Regoli, Francescoin digestive gland
26 Total oxyradical scavenging capacity against peroxyl radicals, unit per protein massTOSC-ROOU/mgRegoli, Francescoin digestive gland
27 Total oxyradical scavenging capacity against peroxyl radicals, unit per protein mass, standard deviationTOSC-ROO std dev±Regoli, Francescoin digestive gland
28 Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein massTOSC-HOU/mgRegoli, Francescoin digestive gland
29 Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein mass, standard deviationTOSC-HO std dev±Regoli, Francescoin digestive gland
30 CadmiumCdµg/gRegoli, Francescoin gills, per dry mass
31 Cadmium, standard deviationCd std dev±Regoli, Francescoin gills, per dry mass
32 Metallothionein, per protein massMT/protnmol/mgRegoli, Francescoin gills
33 Metallothionein per protein, standard deviationMT std dev±Regoli, Francescoin gills
34 Glutathione, total, per unit wet massTGSHµmol/gRegoli, Francescoin gills
35 Glutathione, total, per unit wet mass, standard deviationTGSH std dev±Regoli, Francescoin gills
36 Catalase activity, per protein massCAT/protµmol/min/mgRegoli, Francescoin gills
37 Catalase activity, standard deviationCAT std dev±Regoli, Francescoin gills
38 Glutathione S-transferase, activity per protein massGST/protnmol/min/mgRegoli, Francescoin gills
39 Glutathione S-transferase, activity per protein mass, standard deviationGST std dev±Regoli, Francescoin gills
40 Glutathione reductase, per protein massGR/protnmol/min/mgRegoli, Francescoin gills
41 Glutathione reductase, unit per protein mass, standard deviationGR std dev±Regoli, Francescoin gills
42 Selenium dependent glutathione peroxidases activity, per protein massGPxSe-Dep/protnmol/min/mgRegoli, Francescoin gills
43 Selenium dependent glutathione peroxidases activity, unit per protein mass, standard deviationGPxSe-Dep std dev±Regoli, Francescoin gills
44 Total glutathione peroxidases activity, per protein massGPxTOT/protnmol/min/mgRegoli, Francescoin gills
45 Total glutathione peroxidases activity, unit per protein mass, standard deviationGPxTOT std dev±Regoli, Francescoin gills
46 Total oxyradical scavenging capacity against peroxyl radicals, unit per protein massTOSC-ROOU/mgRegoli, Francescoin gills
47 Total oxyradical scavenging capacity against peroxyl radicals, unit per protein mass, standard deviationTOSC-ROO std dev±Regoli, Francescoin gills
48 Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein massTOSC-HOU/mgRegoli, Francescoin gills
49 Total oxyradical scavenging capacity against hydroxyl radicals, unit per protein mass, standard deviationTOSC-HO std dev±Regoli, Francescoin gills
50 Hemocytes lysosomal membranes stabilityLMSminRegoli, Francesco
51 Hemocytes lysosomal membranes stability, standard deviationLMS std dev±Regoli, Francesco
52 Hemocytes DNA strand breaksDNA damage%Regoli, Francesco
53 Hemocytes DNA strand breaks, standard deviationDNA damage std dev±Regoli, Francesco
54 Hemocytes micronuclei frequencyHem MN‰Regoli, Francesco
55 Hemocytes micronuclei frequency, standard deviationHem MN std dev±Regoli, Francesco
56 Temperature, waterTemp°CRegoli, Francesco
57 Temperature, water, standard deviationTemp std dev±Regoli, Francesco
58 pHpHRegoli, FrancescoPotentiometricNBS scale
59 pH, standard deviationpH std dev±Regoli, FrancescoPotentiometricNBS scale
60 Alkalinity, totalATµmol/kgRegoli, FrancescoPotentiometric titration
61 Alkalinity, total, standard deviationAT std dev±Regoli, FrancescoPotentiometric titration
62 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmRegoli, FrancescoCalculated using CO2SYS
63 Partial pressure of carbon dioxide, standard deviationpCO2 std dev±Regoli, FrancescoCalculated using CO2SYS
64 Calcite saturation stateOmega CalRegoli, FrancescoCalculated using CO2SYS
65 Calcite saturation state, standard deviationOmega Cal std dev±Regoli, FrancescoCalculated using CO2SYS
66 Aragonite saturation stateOmega ArgRegoli, FrancescoCalculated using CO2SYS
67 Aragonite saturation state, standard deviationOmega Arg std dev±Regoli, FrancescoCalculated using CO2SYS
68 Carbonate system computation flagCSC flagYang, YanCalculated using seacarb after Nisumaa et al. (2010)
69 pHpHYang, YanCalculated using seacarb after Nisumaa et al. (2010)total scale
70 Carbon dioxideCO2µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
71 Fugacity of carbon dioxide (water) at sea surface temperature (wet air)fCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
72 Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)pCO2water_SST_wetµatmYang, YanCalculated using seacarb after Nisumaa et al. (2010)
73 Bicarbonate ion[HCO3]-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
74 Carbonate ion[CO3]2-µmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
75 Carbon, inorganic, dissolvedDICµmol/kgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
76 Aragonite saturation stateOmega ArgYang, YanCalculated using seacarb after Nisumaa et al. (2010)
77 Calcite saturation stateOmega CalYang, YanCalculated using seacarb after Nisumaa et al. (2010)
License:
Creative Commons Attribution 3.0 Unported (CC-BY-3.0)
Status:
Curation Level: Enhanced curation (CurationLevelC)
Size:
308 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 
Type
(study)		2 
Species		3 
Reg spec no		4 
URL ref
(WoRMS Aphia ID)		5 
Treat		6 
ID
(label)		7 
Exp duration [days]		8 
Temp [°C]		9 
Sal std dev [±]		10 
Cd [µg/g]
(in digestive gland, per dry mass)		11 
Cd std dev [±]
(in digestive gland, per dry mass)		12 
MT/prot [nmol/mg]
(in digestive gland)		13 
MT std dev [±]
(in digestive gland)		14 
TGSH [µmol/g]
(in digestive gland)		15 
TGSH std dev [±]
(in digestive gland)		16 
CAT/prot [µmol/min/mg]
(in digestive gland)		17 
CAT std dev [±]
(in digestive gland)		18 
GST/prot [nmol/min/mg]
(in digestive gland)		19 
GST std dev [±]
(in digestive gland)		20 
GR/prot [nmol/min/mg]
(in digestive gland)		21 
GR std dev [±]
(in digestive gland)		22 
GPxSe-Dep/prot [nmol/min/mg]
(in digestive gland)		23 
GPxSe-Dep std dev [±]
(in digestive gland)		24 
GPxTOT/prot [nmol/min/mg]
(in digestive gland)		25 
GPxTOT std dev [±]
(in digestive gland)		26 
TOSC-ROO [U/mg]
(in digestive gland)		27 
TOSC-ROO std dev [±]
(in digestive gland)		28 
TOSC-HO [U/mg]
(in digestive gland)		29 
TOSC-HO std dev [±]
(in digestive gland)		30 
Cd [µg/g]
(in gills, per dry mass)		31 
Cd std dev [±]
(in gills, per dry mass)		32 
MT/prot [nmol/mg]
(in gills)		33 
MT std dev [±]
(in gills)		34 
TGSH [µmol/g]
(in gills)		35 
TGSH std dev [±]
(in gills)		36 
CAT/prot [µmol/min/mg]
(in gills)		37 
CAT std dev [±]
(in gills)		38 
GST/prot [nmol/min/mg]
(in gills)		39 
GST std dev [±]
(in gills)		40 
GR/prot [nmol/min/mg]
(in gills)		41 
GR std dev [±]
(in gills)		42 
GPxSe-Dep/prot [nmol/min/mg]
(in gills)		43 
GPxSe-Dep std dev [±]
(in gills)		44 
GPxTOT/prot [nmol/min/mg]
(in gills)		45 
GPxTOT std dev [±]
(in gills)		46 
TOSC-ROO [U/mg]
(in gills)		47 
TOSC-ROO std dev [±]
(in gills)		48 
TOSC-HO [U/mg]
(in gills)		49 
TOSC-HO std dev [±]
(in gills)		50 
LMS [min]		51 
LMS std dev [±]		52 
DNA damage [%]		53 
DNA damage std dev [±]		54 
Hem MN [‰]		55 
Hem MN std dev [±]		56 
Temp [°C]		57 
Temp std dev [±]		58 
pH
(NBS scale, Potentiometric)		59 
pH std dev [±]
(NBS scale, Potentiometric)		60 
AT [µmol/kg]
(Potentiometric titration)		61 
AT std dev [±]
(Potentiometric titration)		62 
pCO2water_SST_wet [µatm]
(Calculated using CO2SYS)		63 
pCO2 std dev [±]
(Calculated using CO2SYS)		64 
Omega Cal
(Calculated using CO2SYS)		65 
Omega Cal std dev [±]
(Calculated using CO2SYS)		66 
Omega Arg
(Calculated using CO2SYS)		67 
Omega Arg std dev [±]
(Calculated using CO2SYS)		68 
CSC flag
(Calculated using seacarb afte...)		69 
pH
(total scale, Calculated using...)		70 
CO2 [µmol/kg]
(Calculated using seacarb afte...)		71 
fCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		72 
pCO2water_SST_wet [µatm]
(Calculated using seacarb afte...)		73 
[HCO3]- [µmol/kg]
(Calculated using seacarb afte...)		74 
[CO3]2- [µmol/kg]
(Calculated using seacarb afte...)		75 
DIC [µmol/kg]
(Calculated using seacarb afte...)		76 
Omega Arg
(Calculated using seacarb afte...)		77 
Omega Cal
(Calculated using seacarb afte...)
laboratoryFlexopecten glaber (mollusk)236720marinespecies.orgCONTROLCTRL10300.514.5441.4028.3601.3300.3310.05519.815.7362673.66304.565643.1711.617409.080.744130.205.318550.11248.357420.43324.1880.160.043.260.120.2760.07646.888.50581.3830.13539.143.462315.063.630034.877.5145236.58416.736216.70327.91348.39716.07629.118.460.620.4820.020.088.210.043464.9138.8407.720.18.60.45.50.2268.0819.34581.15583.122845.86272.083137.284.336.75
laboratoryFlexopecten glaber (mollusk)236720marinespecies.orgCONTROL + CdCd10300.522.8662.0747.6901.0500.5520.09825.626.0293613.45675.902246.965.455908.891.397431.634.713467.75054.619404.60338.6709.351.523.930.430.3950.07933.333.24451.82242.25151.355.51335.333.949542.9418.4109230.77833.685202.43322.92750.5569.26629.4010.102.740.9120.000.108.190.043482.580.1409.322.58.60.35.50.3268.0620.53616.57618.672884.11263.163167.794.196.52
laboratoryFlexopecten glaber (mollusk)236720marinespecies.orgREDUCED pHA10300.518.5001.1579.3252.4070.5410.08247.8510.9986191.72141.477751.635.3658212.690.738041.155.442481.76745.103371.13736.7200.560.112.491.140.3320.08067.9721.41357.2547.10254.4411.619410.022.828528.721.8079154.29735.387202.13847.33141.34012.36224.413.373.640.5719.980.067.410.043431.2179.13144.6160.21.70.11.10.1267.28142.534278.314292.953316.9650.123509.610.801.24
laboratoryFlexopecten glaber (mollusk)236720marinespecies.orgREDUCED pH + CdA-Cd10300.528.6755.6398.9000.6800.4600.07146.8515.4544195.59437.262357.086.7139710.171.755735.933.634481.50045.005383.04231.80919.9813.653.810.270.4420.14070.388.79317.6921.12456.6310.612512.983.071829.586.8751167.38421.654210.88147.65227.64516.18530.676.423.251.2520.920.087.430.043464.9138.83124.3162.31.70.21.10.1267.30134.984158.304172.353340.8854.523530.370.871.35