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Bi, Rong; Ismar, Stefanie M; Sommer, Ulrich; Zhao, Meixun (2018): Experiment on the influence of temperature, N:P supply ratio and pCO2 on elemental composition and growth rates of Emiliania huxleyi. PANGAEA, https://doi.org/10.1594/PANGAEA.887513, Supplement to: Bi, R et al. (2018): Simultaneous shifts in elemental stoichiometry and fatty acids of Emiliania huxleyi in response to environmental changes. Biogeosciences, 15(4), 1029-1045, https://doi.org/10.5194/bg-15-1029-2018

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Abstract:
Climate-driven changes in environmental conditions have significant and complex effects on marine ecosystems. Variability in phytoplankton elements and biochemicals can be important for global ocean biogeochemistry and ecological functions, while there is currently limited understanding on how elements and biochemicals respond to the changing environments in key coccolithophore species such as Emiliania huxleyi. We investigated responses of elemental stoichiometry and fatty acids (FAs) in a strain of E. huxleyi under three temperatures (12, 18 and 24 oC), three N:P supply ratios (molar ratios 10:1, 24:1 and 63:1) and two pCO2 levels (560 and 2400 µatm). Overall, C:N:P stoichiometry showed the most pronounced response to N:P supply ratios, with high ratios of particulate organic carbon vs. particulate organic nitrogen (POC:PON) and low ratios of PON vs. particulate organic phosphorus (PON:POP) in low N-media, and high POC:POP and PON:POP in low P-media. The ratio of particulate inorganic carbon vs. POC (PIC:POC) and polyunsaturated fatty acid proportions strongly responded to temperature and pCO2, both being lower under high pCO2 and higher with warming. We observed synergistic interactions between warming and nutrient deficiency (and high pCO2) on elemental cellular contents and docosahexaenoic acid (DHA) proportion in most cases, indicating the enhanced effect of warming under nutrient deficiency (and high pCO2). Our results suggest differential sensitivity of elements and FAs to the changes in temperature, nutrient availability and pCO2 in E. huxleyi, which is to some extent unique compared to non-calcifying algal classes. Thus, simultaneous changes of elements and FAs should be considered when predicting future roles of E. huxleyi in the biotic-mediated connection between biogeochemical cycles, ecological functions and climate change.
Parameter(s):
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1FigureFigBi, Rong
2SpeciesSpeciesBi, Rong
3Treatment: temperatureTreat temp°CBi, Rong
4Treatment: partial pressure of carbon dioxideTreat pCO2µatmBi, Rong
5Treatment: nutrientsT:nutrientsBi, RongN : P supply ratio
6Growth rateµ#/dayBi, Rongmax
7Carbon, organic, particulate, per cellPOCpg/#Bi, Rong
8Nitrogen, organic, particulate, per cellPONpg/#Bi, Rong
9Phosphorus, organic, particulate, per cellPOPpg/#Bi, Rong
10Carbon, inorganic, particulate, per cellPIC/cellpg/#Bi, Rong
11Carbon, organic, particulate/Nitrogen, organic, particulate ratioPOC/PONBi, Rong
12Particulate organic carbon/particulate organic phosphorus ratioPOC/POPBi, Rong
13Particulate organic nitrogen/particulate organic phosphorus ratioPON/POPBi, Rong
14Particulate inorganic carbon/particulate organic carbon ratioPIC/POCBi, Rong
15Carbon, inorganic, particulate, population yieldPIC pop yieldµg/mlBi, Rong
16Carbon, organic, particulate, population yieldPOC pop yieldµg/mlBi, Rong
17Particulate inorganic carbon production per cellPIC prodpg/#/dayBi, Rong
18Particulate organic carbon production per cellPOC prodpg/#/dayBi, Rong
Size:
1025 data points

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