Not logged in
Data Publisher for Earth & Environmental Science

Ramesh, Kirti; Melzner, Frank; Griffith, Andrew W; Gobler, Christopher J; Rouger, Caroline; Tasdemir, Deniz; Nehrke, Gernot (2018): Raman and FTIR spectra of larval bivalve shells. PANGAEA,, Supplement to: Ramesh, K et al. (2018): In vivo characterization of bivalve larval shells: a confocal Raman microscopy study. Journal of The Royal Society Interface, 15(141), 20170723,

Always quote above citation when using data! You can download the citation in several formats below.

RIS CitationBibTeX Citation

In vivo confocal Raman microscopy (CRM), polarized light microscopy and Fourier transform infrared spectroscopy (FTIR) were used to determine if a significant amount of amorphous calcium carbonate (ACC) exists within larval shells of Baltic mytilid mussels (Mytilus edulis-like) and whether the amount of ACC varies during larval development. No evidence for ACC was found from the onset of shell deposition at 21 h post-fertilization (hpf) until 48 hpf. Larval Mytilus shells were crystalline from 21 hpf onwards and exhibited CRM and FTIR peaks characteristic of aragonite. Prior to shell deposition at 21 hpf, no evidence for carbonates was observed through in vivo CRM. We further analysed the composition of larval shells in three other bivalve species, Mercenaria mercenaria, Crassostrea gigas and Crassostrea virginica and observed no evidence for ACC, which is in contrast to previous work on the same species. Our findings indicate that larval bivalve shells are composed of crystalline aragonite and we demonstrate that conflicting results are related to sub-optimal measurements and misinterpretation of CRM spectra. Our results demonstrate that the common perception that ACC generally occurs as a stable and abundant precursor during larval bivalve calcification needs to be critically reviewed.
#NameShort NameUnitPrincipal InvestigatorMethod/DeviceComment
1Sample code/labelSample labelRamesh, Kirti
2SpeciesSpeciesRamesh, Kirti
3Wave numberW no1/cmRamesh, KirtiRaman shift
4Transmission of lightTransmission%Ramesh, Kirti
5Analytical methodMethodRamesh, KirtiConfocal Raman microscopy (CRM); Fourier Transform infrared spectroscopy (FTIR)
143020 data points

Download Data

Download dataset as tab-delimited text (use the following character encoding: )

View dataset as HTML (shows only first 2000 rows)