Citation:

bg-15-581-2018

Name: Seawater carbonate chemistry and elemental composition of a southern hemisphere strain of coccolithophore Emiliania huxleyi
Published: 2018-01-23
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species

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Abstract:

A series of semi-continuous incubation experiments were conducted with the coccolithophore Emiliania huxleyi strain NIWA1108 (Southern Ocean isolate) to examine the effects of five environmental drivers (nitrate concentration, phosphate concentration, irradiance, temperature and pCO2) on both the physiological rates and elemental composition. Here, we report the alteration of the elemental composition of E. huxleyi in response to the changes in these environmental drivers. A series of dose response curves for the cellular elemental composition of E. huxleyi were fitted for each of the five drivers across an environmentally-representative gradient. The importance of each driver in regulating the elemental composition of E. huxleyi was ranked using a semi-quantitative approach. The percentage variation in elemental composition arising from the change in each driver between present day and model-projected conditions for the year 2100 were calculated. Temperature was the most important driver controlling both cellular particulate organic and inorganic carbon content, whereas nutrient concentrations were the most important regulator of cellular particulate nitrogen and phosphorus of E. huxleyi. In contrast, elevated pCO2 had the greatest influence on cellular particulate inorganic carbon to organic carbon ratio, resulting in a decrease in the ratio. Our results indicate that the different environmental drivers each play specific roles in regulating the cellular elemental composition of E. huxleyi with wide-reaching implications for coccolithophore biogeochemistry, as a consequence of the regulation of E. huxleyi physiological processes.

Keyword(s):

Biomass/Abundance/Elemental composition; Bottles or small containers/Aquaria (<20 L); Chromista; Emiliania huxleyi; Growth/Morphology; Haptophyta; Laboratory experiment; Laboratory strains; Not applicable; Pelagos; Phytoplankton; Single species

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2018-01-23.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Feng, Yuanyuan		study
2	Species	Species		Feng, Yuanyuan
3	Registration number of species	Reg spec no		Feng, Yuanyuan
4	Uniform resource locator/link to reference	URL ref		Feng, Yuanyuan		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Feng, Yuanyuan
6	Phosphate	[PO4]3-	µmol/l	Feng, Yuanyuan
7	Nitrate	[NO3]-	µmol/l	Feng, Yuanyuan
8	Irradiance	E	µmol/m²/s	Feng, Yuanyuan
9	Temperature, water	Temp	°C	Feng, Yuanyuan
10	Carbon dioxide, partial pressure	pCO2	Pa	Feng, Yuanyuan
11	Growth rate	µ	1/day	Feng, Yuanyuan
12	Growth rate	µ	1/h	Feng, Yuanyuan
13	Carbon, organic, particulate, per cell	POC/cell	pg/#	Feng, Yuanyuan
14	Carbon, organic, particulate, standard deviation	POC std dev	±	Feng, Yuanyuan
15	Carbon, inorganic, particulate, per cell	PIC/cell	pg/#	Feng, Yuanyuan
16	Particulate inorganic carbon per cell, standard deviation	PIC/cell std dev	±	Feng, Yuanyuan
17	Particulate inorganic carbon/particulate organic carbon ratio	PIC/POC		Feng, Yuanyuan
18	Particulate inorganic carbon/particulate organic carbon ratio, standard deviation	PIC/POC ratio std dev	±	Feng, Yuanyuan
19	Particulate organic nitrogen per cell	PON/cell	pg/#	Feng, Yuanyuan
20	Nitrogen, organic, particulate, per cell, standard deviation	PON/cell std dev	±	Feng, Yuanyuan
21	Phosphorus, organic, particulate, per cell	POP/cell	pg/#	Feng, Yuanyuan
22	Phosphorus, organic, particulate, per cell, standard deviation	POP/cell std dev	±	Feng, Yuanyuan
23	Carbon, organic, particulate/chlorophyll a ratio	POC/Chl a	g/g	Feng, Yuanyuan
24	Carbon, organic, particulate/chlorophyll a ratio, standard deviation	POC/Chl a std dev	±	Feng, Yuanyuan
25	Salinity	Sal		Feng, Yuanyuan
26	pH	pH		Feng, Yuanyuan		total scale
27	pH, standard deviation	pH std dev	±	Feng, Yuanyuan		total scale
28	Carbon, inorganic, dissolved	DIC	µmol/kg	Feng, Yuanyuan
29	Carbon, inorganic, dissolved, standard deviation	DIC std dev	±	Feng, Yuanyuan
30	Alkalinity, total	AT	µmol/kg	Feng, Yuanyuan
31	Alkalinity, total, standard deviation	AT std dev	±	Feng, Yuanyuan
32	Carbon dioxide, partial pressure	pCO2	Pa	Feng, Yuanyuan
33	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Feng, Yuanyuan
34	Bicarbonate ion	[HCO3]-	µmol/kg	Feng, Yuanyuan
35	Bicarbonate ion, standard deviation	[HCO3]- std dev	±	Feng, Yuanyuan
36	Carbonate ion	[CO3]2-	µmol/kg	Feng, Yuanyuan
37	Carbonate ion, standard deviation	[CO3]2- std dev	±	Feng, Yuanyuan
38	Carbon dioxide	CO2	µmol/kg	Feng, Yuanyuan
39	Carbon dioxide, standard deviation	CO2 std dev	±	Feng, Yuanyuan
40	Calcite saturation state	Omega Cal		Feng, Yuanyuan
41	Calcite saturation state, standard deviation	Omega Cal std dev	±	Feng, Yuanyuan
42	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
44	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

758 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 [PO4]3- [µmol/l]	7 [NO3]- [µmol/l]	8 E [µmol/m²/s]	9 Temp [°C]	10 pCO2 [Pa]	11 µ [1/day]	12 µ [1/h]	13 POC/cell [pg/#]	14 POC std dev [±]	15 PIC/cell [pg/#]	16 PIC/cell std dev [±]	17 PIC/POC	18 PIC/POC ratio std dev [±]	19 PON/cell [pg/#]	20 PON/cell std dev [±]	21 POP/cell [pg/#]	22 POP/cell std dev [±]	23 POC/Chl a [g/g]	24 POC/Chl a std dev [±]	25 Sal	26 pH (total scale)	27 pH std dev [±] (total scale)	28 DIC [µmol/kg]	29 DIC std dev [±]	30 AT [µmol/kg]	31 AT std dev [±]	32 pCO2 [Pa]	33 pCO2 std dev [±]	34 [HCO3]- [µmol/kg]	35 [HCO3]- std dev [±]	36 [CO3]2- [µmol/kg]	37 [CO3]2- std dev [±]	38 CO2 [µmol/kg]	39 CO2 std dev [±]	40 Omega Cal	41 Omega Cal std dev [±]	42 CSC flag	43 CO2 [µmol/kg]	44 fCO2water_SST_wet [µatm]	45 pCO2water_SST_wet [µatm]	46 [HCO3]- [µmol/kg]	47 [CO3]2- [µmol/kg]	48 DIC [µmol/kg]	49 Omega Arg	50 Omega Cal
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20	0.4					0.547	0.009	9.913	0.969	6.620	1.069	0.673	0.131	1.829	0.074	0.098	0.011	75.265	4.390	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20	2.0					0.644	0.007	11.235	0.558	10.481	3.642	0.926	0.287	1.690	0.774	0.212	0.029	75.627	2.847	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20	6.0					0.647	0.003	10.382	0.468	11.886	3.695	1.138	0.305	1.999	0.314	0.307	0.027	59.844	0.839	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20	10.0					0.644	0.007	11.449	0.049	10.250	1.207	0.895	0.102	2.550	0.017	0.336	0.027	68.584	4.464	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20	20.0					0.645	0.008	10.578	0.111	9.198	1.805	0.869	0.169	2.362	0.033	0.374	0.020	60.325	3.675	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20		3.7				0.100	0.012	11.578	0.802	11.241	0.027	0.955	0.077	0.889	0.235	0.217	0.016	422.364	74.284	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20		6.0				0.257	0.017	9.853	2.443	12.356		1.067		0.886	0.293	0.207	0.027	218.133	62.578	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20		12.0				0.586	0.006	12.792	2.089	7.861	2.085	0.609	0.064	1.659	0.284	0.227	0.015	95.306	6.342	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20		48.0				0.781	0.002	12.571	1.860	10.929	2.214	0.871	0.122	1.894	0.476	0.302	0.043	55.512	4.282	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20		96.0				0.784	0.013	12.117	0.189	9.790	0.940	0.808	0.071	2.190	0.312	0.303	0.023	54.814	5.172	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20		200.0				0.781	0.018	13.284	2.755	11.700	1.683	0.924	0.329	2.105	0.335	0.325	0.044	55.144	4.160	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20			650			0.637	0.028	12.507	1.511	11.571	1.297	0.927	0.047	2.372	0.283	0.326	0.026	114.212	5.253	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20			350			0.820	0.008	13.820	0.443	11.962	1.689	0.865	0.107	2.507	0.256	0.323	0.020	80.457	5.882	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20			190			0.829	0.029	15.779	3.566	12.150	2.137	0.715	0.105	3.358	0.752	0.403	0.122	75.969	11.360	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20			80			0.780	0.004	14.074	1.169	11.028	0.771	0.790	0.115	3.146	0.221	0.441	0.061	56.294	7.282	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20			40			0.622	0.014	9.971		10.168		1.020		3.366		0.487	0.091	53.820		34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20			14			0.381	0.009	8.199	2.391	9.682	2.216	1.196	0.090	2.989	0.991	0.319	0.153	47.448	12.582	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				4		0.070	0.024	28.853	6.983	13.235	3.997	0.455	0.028	4.074	0.004	1.080	0.146	131.261	42.962	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				7		0.237	0.022	20.116	2.355	16.335	5.630	0.812	0.281	3.152	0.638	0.668	0.078	70.052	8.846	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				11		0.665	0.008	12.642	0.621	14.812	2.944	1.181	0.282	2.398	0.031	0.381	0.043	59.224	2.918	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				15		0.932	0.028	11.085	0.142	9.531	0.749	0.860	0.062	1.934	0.097	0.303	0.016	60.190	6.118	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				20		1.156	0.005	7.982	0.657	7.103	0.581	0.890	0.011	1.557	0.137	0.222	0.006	46.179	1.265	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				25		1.214	0.008	7.773	0.382	7.329	0.737	0.946	0.132	1.312	0.243	0.214	0.017	47.775	1.530	34.500
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				14	8.00	0.856	0.018	9.629	1.668	13.850	0.310	1.457	0.220	1.785	0.424	0.203	0.037	50.163	4.430	34.500	8.498	0.020	1690	12	2160	25	10	1	1378	13	308	12	3.9	0.2	7.4	0.3	9	3.92	101.30	101.67	1379.24	306.84	2150.70	4.70	7.34
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				14	15.20	0.979	0.053	12.932	1.843	14.290	1.078	1.163	0.125	2.604	0.266	0.249	0.051	53.177	8.346	34.500	8.301	0.025	1778	23	2113	12	18	1	1550	30	221	9	6.9	0.5	5.3	0.2	9	6.95	179.38	180.04	1551.73	219.32	2105.53	3.36	5.24
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				14	38.71	0.958	0.050	15.601	3.815	12.894	2.333	0.896	0.147	2.023	0.757	0.259	0.044	80.769	12.773	34.500	8.042	0.020	1914	48	2116	42	37	3	1762	49	138	3	14.0	1.0	3.3	0.1	9	14.33	369.90	371.26	1762.46	137.21	2108.76	2.10	3.28
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				14	57.55	0.958	0.044	15.399	2.676	11.532	2.710	0.770	0.251	2.767	0.485	0.341	0.046	72.298	3.727	34.500	7.849	0.031	2031	19	2158	30	63	4	1910	14	96	8	24.0	2.0	2.3	0.2	9	24.23	625.62	627.92	1911.36	95.41	2151.15	1.46	2.28
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				14	74.27	0.934	0.032	15.323	3.185	13.106	2.235	0.860	0.039	2.774	0.471	0.337	0.032	66.560	6.884	34.500	7.748	0.046	2086	5	2180	16	83	9	1976	7	79	8	32.0	3.0	1.9	0.2	9	31.61	816.21	819.21	1976.21	78.18	2172.29	1.20	1.87
laboratory	Emiliania huxleyi (phytoplankton)	115104	marinespecies.org	20				14	109.43	0.892	0.010	14.776	2.979	11.928	1.433	0.821	0.129	2.341	0.406	0.384	0.019	75.057	10.097	34.500	7.624	0.023	2122	8	2176	15	113	6	2019	7	60	3	43.0	2.0	1.4	0.1	9	42.97	1109.44	1113.52	2019.00	60.04	2169.60	0.92	1.44