Citation:

PANGAEA.882015

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Abstract:

Fluorescence in situ hybridization (FISH) with 16S rRNA-targeted oligonucleotide probes was used to investigate the phylogenetic composition of the seven most abundant bacterial clades (determined by 16S rRNA tag sequencing) of the South Pacific Gyre (SPG). Seawater samples were collected aboard the RV Sonne SO-245 "UltraPac"cruise from Antofagasta, Chile (17.12.2015) to Wellington, New Zealand (28.01.2016). A total of 15 stations were sampled at multiple depths from surface (20 m) to ~5000 m. Total cell counts (TCC, by DAPI staining) and FISH were carried out as described in Bennke et al. (2016). DAPI and FISH stained cells were visualised and counted automatically using a fully automated image acquisition and cell enumeration system (Bennke et al., 2016).

The cellular abundance of SAR11 clade, Prochlorococcus, AEGEAN-169 marine group, SAR86, SAR202, SAR324 and SAR406 were enumerated and are shown here in total (cell ml-1) and relative abundance (% TCC). For this study, a new probe specific for the AEGEAN-169 clade was designed and tested, based on the latest SILVA 16S rRNA database (refnr 128). We found that the microbial community within the SPG was highly similar to that of other oceanic gyres and showed a pronounced vertical distribution pattern. Two major differences which we observed, in comparison to previous studies of both the SPG and other oceanic Gyres, was a high abundance of the AEGEAN-169 marine group, a sister group of the SAR11 clade, and a low abundance of Prochlorococcus specifically in the surface waters of the central gyre.

Supplement to:

Reintjes, Greta; Tegetmeyer, Halina E; Bürgisser, Miriam; Orlic, Sandi; Tews, Ivo; Zubkov, Mikhail V; Voß, Daniela; Zielinski, Oliver; Quast, Christian; Glöckner, Frank Oliver; Amann, Rudolf; Ferdelman, Timothy G; Fuchs, Bernhard M (2019): On-Site Analysis of Bacterial Communities of the Ultraoligotrophic South Pacific Gyre. Applied and Environmental Microbiology, 85(14), https://doi.org/10.1128/AEM.00184-19

Related to:

Reintjes, Greta; Amann, Rudolf I; Arnosti, Carol; Fuchs, Bernhard M (2020): Microbial community composition and polysaccharide processing potential in the South Pacific Gyre. European Nucleotide Archive (ENA), insdc:PRJEB40503

Further details:

Oligonucleotide probes and formamide concentration used in Reintjes etal., 2017

Coverage:

Median Latitude: -30.101353 * Median Longitude: -121.254259 * South-bound Latitude: -39.310400 * West-bound Longitude: -170.000180 * North-bound Latitude: -23.491900 * East-bound Longitude: -84.562420

Date/Time Start: 2015-12-25T18:03:00 * Date/Time End: 2016-01-24T04:30:00

Minimum DEPTH, water: 5 m * Maximum DEPTH, water: 5125 m

Event(s):

SO245_1-1 * Latitude: -23.503170 * Longitude: -84.562420 * Date/Time: 2015-12-25T18:03:00 * Elevation: -2764.4 m * Location: South Pacific Ocean * Campaign: (UltraPac, GEOTRACES) * Basis: * Method/Device: (CTD-RO)

SO245_2-2 * Latitude: -23.491900 * Longitude: -90.029850 * Date/Time: 2015-12-27T07:25:00 * Elevation: -3890.9 m * Location: South Pacific Ocean * Campaign: (UltraPac, GEOTRACES) * Basis: * Method/Device: (CTD-RO)

SO245_3-1 * Latitude: -23.499400 * Longitude: -95.295300 * Date/Time: 2015-12-29T18:03:00 * Elevation: -3772.3 m * Location: South Pacific Ocean * Campaign: (UltraPac, GEOTRACES) * Basis: * Method/Device: (CTD-RO)

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Event label	Event		Reintjes, Greta
2	Number	No		Reintjes, Greta
3	Station label	Station		Reintjes, Greta
4	DEPTH, water	Depth water	m	Reintjes, Greta		Geocode
5	SAR11 mix oligonucleotide FISH-probes targeting SAR11 clade	SAR11	%	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
6	Prochlorococcus, targeted with PRO405 oligonucleotide FISH-probe	PRO405	%	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
7	AEGEAN-169 marine group, targeted with the AEGEAN-169 mix oligonucleotide FISH-probes	AEGEAN-169	%	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
8	SAR86 clade, targeted with SAR86-1245 oligonucleotide FISH-probe	SAR86-1245	%	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
9	SAR202 clade, targeted with SAR202-312R oligonucleotide FISH-probe	SAR202-312R	%	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
10	SAR324 clade, targeted with SAR324-1412 oligonucleotide FISH-probe	SAR324-1412	%	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
11	SAR406 clade, targeted with SAR406-97 oligonucleotide FISH-probe	SAR406-97	%	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
12	Microbial abundance, cells	Microbial abund	#/ml	Reintjes, Greta	Flow cytometry	Total cell counts (enumerated by DAPI) counted by flow cyctometry (FCM is flow cytometry)
13	SAR11 mix oligonucleotide FISH-probes targeting SAR11 clade	SAR11	#/ml	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
14	Prochlorococcus, targeted with PRO405 oligonucleotide FISH-probe	PRO405	#/ml	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
15	AEGEAN-169 marine group, targeted with the AEGEAN-169 mix oligonucleotide FISH-probes	AEGEAN-169	#/ml	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
16	SAR86 clade, targeted with SAR86-1245 oligonucleotide FISH-probe	SAR86	#/ml	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
17	SAR202 clade, targeted with SAR202-312R oligonucleotide FISH-probe	SAR202-312R	#/ml	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
18	SAR324 clade, targeted with SAR324-1412 oligonucleotide FISH-probe	SAR324	#/ml	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
19	SAR406 clade, targeted with SAR406-97 oligonucleotide FISH-probe	SAR406	#/ml	Reintjes, Greta	Catalysed reporter deposition-fluorescence in situ hybridization (CARD-FISH)
20	Accession number, genetics	Access no gen		Reintjes, Greta

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Size:

2922 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Event	2 No	3 Station	4 Depth water [m]	5 SAR11 [%]	6 PRO405 [%]	7 AEGEAN-169 [%]	8 SAR86-1245 [%]	9 SAR202-312R [%]	10 SAR324-1412 [%]	11 SAR406-97 [%]	12 Microbial abund [#/ml]	13 SAR11 [#/ml]	14 PRO405 [#/ml]	15 AEGEAN-169 [#/ml]	16 SAR86 [#/ml]	17 SAR202-312R [#/ml]	18 SAR324 [#/ml]	19 SAR406 [#/ml]	20 Access no gen
SO245_1-1	1	SO245-01-01	20	17.0	23.3	3.7	2.1		0.0		593523	100602	138232	21960	12286		59		insdc:ERS1883698
SO245_1-1	1	SO245-01-01	50	14.2	25.0	4.4	1.8	0.1	0.0	0.9	685406	97533	171283	30089	12132	411	206	5826	insdc:ERS1883697
SO245_1-1	1	SO245-01-01	75	9.0	29.2	2.8	1.7		0.1	1.0	659309	59008	192782	18527	11274		462	6329	insdc:ERS1883696
SO245_1-1	1	SO245-01-01	100	19.3	18.8	1.2	1.7	0.6	0.6	1.5	697977	134779	131150	8027	12075	4118	3839	10539	insdc:ERS1883695
SO245_1-1	1	SO245-01-01	125	15.9	8.5	1.1	1.1		1.2	2.5	464972	73698	39430	5208	5254		5626	11531	insdc:ERS1883694
SO245_1-1	1	SO245-01-01	150	25.7	2.3	0.5	0.5	1.4	2.8	2.6	275775	70847	6233	1406	1434	3723	7694	7032	insdc:ERS1883693
SO245_1-1	1	SO245-01-01	400	19.1		0.7		1.5	5.4	2.3	109339	20895		733		1629	5872	2471	insdc:ERS1883692
SO245_1-1	1	SO245-01-01	500	18.1		0.8		1.8	4.9	1.5	108471	19612		900		1974	5261	1660	insdc:ERS1883691
SO245_2-2	2	SO245-02-12	20	27.0	18.3	3.4	1.8		0.0		546193	147199	99789	18516	9995		109		insdc:ERS1883690
SO245_2-2	2	SO245-02-12	50	28.7	19.0	3.6	1.6	0.0	0.0	0.6	792141	227028	150824	28359	12516	238	317	4832	insdc:ERS1883689
SO245_2-2	2	SO245-02-12	100	14.8	23.3	1.0	1.1	0.3	0.5	0.8	1109587	163997	258534	11096	12649	3551	5548	9099	insdc:ERS1883688
SO245_2-2	2	SO245-02-10	125	13.3	10.9	1.5	1.3		0.9	1.2	693089	92250	75547	10258	8664		6446	8248	insdc:ERS1883687
SO245_2-2	2	SO245-02-10	150	17.2	7.0	0.8	0.3		1.7	1.6	396704	68114	27769	3015	1031		6784	6228	insdc:ERS1883686
SO245_2-2	2	SO245-02-10	175	25.1	2.9	0.4	0.3	0.6	3.1	2.0	301403	75682	8831	1236	784	1658	9193	5877	insdc:ERS1883685
SO245_2-2	2	SO245-02-10	200	18.9	2.7	0.6	0.3	2.7	3.7	2.5	156120	29507	4200	859	390	4246	5745	3965	insdc:ERS1883684
SO245_2-2	2	SO245-02-10	250		0.4	0.6	0.2	2.7	4.5		121831		439	743	231	3314	5482		insdc:ERS1883683
SO245_2-2	2	SO245-02-10	300	18.3	0.5	0.9	0.4	4.0	5.3	2.1	122474	22400	661	1041	490	4838	6454	2511	insdc:ERS1883682
SO245_2-2	2	SO245-02-05	500	18.0	0.3	0.5		2.5	4.5	2.8	103280	18570	310	485		2603	4679	2882	insdc:ERS1883681
SO245_2-2	2	SO245-02-05	1000	14.8	0.7	0.4		3.3	4.6	3.4	54836	8094	384	236		1793	2539	1886	insdc:ERS1883680
SO245_2-2	2	SO245-02-02	1500	18.4	1.1	0.6		3.9	5.2	2.4	41561	7635	470	266		1600	2149	1002	insdc:ERS1883679
SO245_2-2	2	SO245-02-02	2200	16.6		0.5		6.1	4.5	4.2	43108	7160		198		2643	1953	1802	insdc:ERS1883678
SO245_2-2	2	SO245-02-02	3500	13.0		0.8		7.2	5.4	3.3	31707	4125		263		2280	1699	1040	insdc:ERS1883677
SO245_3-1	3	SO245-03-01	50	13.3	11.8	2.5	1.1	0.1	0.1	1.2	496592	66047	58548	12564	5422	447	447	5810	insdc:ERS1883676
SO245_3-1	3	SO245-03-01	100	7.8	14.2	1.9	0.8	0.4	0.3	1.2	660263	51633	94021	12347	5245	2905	2245	8121	insdc:ERS1883675
SO245_3-1	3	SO245-03-01	125	3.7	25.7	1.5	0.6		0.6	1.3	531764	19409	136823	7711	3122		3244	6966	insdc:ERS1883674
SO245_3-1	3	SO245-03-01	150	20.2	15.3	1.9	0.4	0.7	1.7	2.2	313088	63275	47777	6074	1101	2098	5385	6982	insdc:ERS1883673
SO245_3-1	3	SO245-03-01	170	16.2	10.2	0.6	0.3	0.7	2.2	1.4	348515	56390	35653	2161	912	2440	7598	4879	insdc:ERS1883672
SO245_3-1	3	SO245-03-01	200	21.1	3.6	0.7	0.1	1.8	3.8	2.0	156752	33137	5627	1050	166	2853	5910	3151	insdc:ERS1883671
SO245_3-1	3	SO245-03-01	300	13.9	0.5	0.8	0.1	3.6	4.7	2.0	107233	14937	504	804	97	3817	5061	2177	insdc:ERS1883670
SO245_3-1	3	SO245-03-01	500	17.0	0.4	0.3		3.2	5.2	1.8	60929	10370	238	195		1931	3168	1115	insdc:ERS1883669
SO245_4-1	4	SO245-04-13	60	46.8	14.0	4.5	1.2	0.0	0.1	0.2	470283	220092	65981	21210	5596	141	329	847
SO245_4-1	4	SO245-04-13	100	35.2	19.4	2.5	1.0	0.4	0.6	1.0	457259	161001	88525	11203	4481	1738	2652	4618	insdc:ERS1883667
SO245_4-1	4	SO245-04-11	150	17.9	31.4	1.1	1.0	0.8	1.0	1.2	435860	78019	136773	4707	4533	3530	4489	5100	insdc:ERS1883666
SO245_4-1	4	SO245-04-11	175	20.4	21.6	1.4	0.3	0.2	1.7	1.3	351659	71633	75888	4888	1160	774	5943	4677	insdc:ERS1883665
SO245_4-1	4	SO245-04-11	200	29.6	12.8	0.7	0.1	0.8	2.2	1.7	262111	77559	33419	1809	367	2123	5662	4535	insdc:ERS1883664
SO245_4-1	4	SO245-04-11	225	32.3	4.4	0.6	0.1	1.5	3.4	2.7	177267	57328	7764	1135	106	2677	5938	4751
SO245_4-1	4	SO245-04-11	250	36.1	1.4	0.7	0.0	1.5	4.8	1.9	149845	54094	2023	1079	0	2263	7163	2907	insdc:ERS1883663
SO245_4-1	4	SO245-04-11	300	29.8	0.0	1.0	0.0	5.2	4.9	2.1	141077	42041	0	1439	28	7350	6927	2892	insdc:ERS1883662
SO245_4-1	4	SO245-04-07	500	19.8	0.0	0.6		2.9	4.8	1.6	257703	50896	0	1495		7525	12447	4123	insdc:ERS1883661
SO245_4-1	4	SO245-04-07	1000	21.8		0.4		3.8	4.1	1.5	57754	12608		214		2183	2339	861	insdc:ERS1883660
SO245_4-1	4	SO245-04-01	2250	14.3		0.2		7.9	4.7	1.6	30929	4414		74		2440	1454	504
SO245_4-1	4	SO245-04-01	3500	12.5		0.2		6.6	5.8	0.7	30721	3840		61		2037	1785	215	insdc:ERS1883659
SO245_4-1	4	SO245-04-01	3900			0.5		7.5	5.7	2.1	32210			151		2422	1830	673	insdc:ERS1883658
SO245_5-1	5	SO245-05-01	5								444464
SO245_5-1	5	SO245-05-01	25	45.4	8.4	5.8	1.7				458832	208114	38312	26520	7892
SO245_5-1	5	SO245-05-01	50	34.0	7.2	4.0	1.5	0.0	0.0	1.1	490292	166842	35399	19612	7550	98	49	5197	insdc:ERS1883657
SO245_5-1	5	SO245-05-01	100	39.3	6.1	1.9	1.5	0.1	0.3	1.1	514007	202116	31303	9766	7505	360	1388	5397	insdc:ERS1883656
SO245_5-1	5	SO245-05-01	150	26.3	15.9	0.9	1.2	0.4	0.9	1.0	480223	126172	76163	4514	5859	1921	4418	4898	insdc:ERS1883655
SO245_5-1	5	SO245-05-01	175	30.6	3.3	0.7	0.3	1.2	3.3	3.0	474804	145495	15479	3181	1519	5698	15431	14244	insdc:ERS1883654
SO245_5-1	5	SO245-05-01	200	24.3	8.3	0.5	0.3	0.6	2.0	2.1	357728	86772	29620	1717	930	2111	7047	7477	insdc:ERS1883653
SO245_5-1	5	SO245-05-01	225	19.0	19.4	1.0	0.8	0.3	1.6	1.0	201711	38295	39112	1916	1513	524	3308	2037	insdc:ERS1883652
SO245_5-1	5	SO245-05-01	250	29.3	3.6	0.4	0.1	4.3	4.1	2.3	153455	44951	5478	675	77	6599	6338	3468	insdc:ERS1883651
SO245_5-1	5	SO245-05-01	300	24.7	0.4	0.4	0.2	5.2	5.0	2.2	136190	33619	558	586	313	7123	6850	2969	insdc:ERS1883650
SO245_5-1	5	SO245-05-01	400								79700
SO245_5-1	5	SO245-05-01	500	16.7		0.6		3.1	4.8	1.7	65586	10931		361		2059	3122	1141	insdc:ERS1883649
SO245_6-1	6	SO245-06-11	5								448238
SO245_6-1	6	SO245-06-11	10								514200
SO245_6-1	6	SO245-06-11	20	50.6	7.8	5.6	2.0				512899	259527	39955	28928	10104				insdc:ERS1883648
SO245_6-1	6	SO245-06-11	40								512008
SO245_6-1	6	SO245-06-11	60	48.0	5.2	4.3	1.2	0.0	0.0	0.6	497332	238471	25662	21186	6117	0	99	2984	insdc:ERS1883647
SO245_6-1	6	SO245-06-11	80								505279
SO245_6-1	6	SO245-06-11	100		9.5			0.2	0.1	0.6	555502		52939			889	778	3277	insdc:ERS1883646
SO245_6-1	6	SO245-06-11	125	43.0	11.0	2.2	0.9		0.6	0.4	566335	243297	62467	12289	4927		3568	2435	insdc:ERS1883645
SO245_6-1	6	SO245-06-08	150	29.5	22.8	1.0	0.9	0.4	0.9	0.9	476912	140689	108831	4912	4054	1717	4149	4292	insdc:ERS1883644
SO245_6-1	6	SO245-06-08	175	28.6	22.3	2.5	1.0	0.8	1.4	1.3	366095	104813	81639	9006	3624	3075	4942	4832	insdc:ERS1883643
SO245_6-1	6	SO245-06-08	200	33.9	12.5	1.3	0.2	0.9	2.2	1.4	303226	102733	37994	3851	637	2638	6641	4154	insdc:ERS1883642
SO245_6-1	6	SO245-06-08	225	42.0	2.7	1.1	0.3	1.0	4.2	1.1	185460	77838	5045	2040	464	1929	7715	2114	insdc:ERS1883641
SO245_6-1	6	SO245-06-08	250	43.2	2.2	0.5	0.6	1.5	3.9	1.3	151205	65305	3311	817	937	2329	5912	2011	insdc:ERS1883640
SO245_6-1	6	SO245-06-08	275
SO245_6-1	6	SO245-06-08	300	28.4	0.5	0.8	0.3	5.6	4.3	1.8	120777	34301	628	906	362	6727	5205	2126	insdc:ERS1883639
SO245_6-1	6	SO245-06-03	500	23.4		0.4		4.0	4.1	1.9									insdc:ERS1883638
SO245_6-1	6	SO245-06-03	1000	19.8				3.0	5.6	2.5									insdc:ERS1883637
SO245_6-1	6	SO245-06-01	1500	12.7				4.2	5.5	2.0									insdc:ERS1883636
SO245_6-1	6	SO245-06-01	2500	12.5				6.6	4.7	1.1
SO245_6-1	6	SO245-06-01	3600	14.3				6.7	4.5
SO245_7-4	7	SO245-07-04	5								391755
SO245_7-4	7	SO245-07-04	20	34.2	8.5	6.0	1.3				479769	164033	40780	28786	6189
SO245_7-4	7	SO245-07-04	50	32.3	7.2	3.3	1.3	0.0	0.1	0.5	470075	151646	33798	15654	6111	188	423	2397
SO245_7-4	7	SO245-07-04	100	34.1	9.0	2.1	1.1	0.1	0.3	0.9
SO245_7-4	7	SO245-07-04	125								501012
SO245_7-4	7	SO245-07-04	150	24.1	22.5	0.8	0.6	0.6	0.9	1.1	437221	105326	98287	3410	2623	2536	3935	4678
SO245_7-4	7	SO245-07-04	175	16.1	27.4	3.9	1.0	0.7	1.2	1.7	427690	68687	116973	16851	4405	2823	4918	7142
SO245_7-4	7	SO245-07-04	200	20.7	14.1	1.0	0.5	1.1	1.6	1.9	323139	66793	45498	3361	1713	3522	5106	6140
SO245_7-4	7	SO245-07-04	225	35.5	8.7	0.5	0.1	1.6	3.5	1.7	221599	78668	19346	1042	310	3612	7845	3745
SO245_7-4	7	SO245-07-04	250	29.5	2.6	0.5	0.1	3.0	4.3	1.9	162105	47870	4231	827	211	4912	6938	2999
SO245_7-4	7	SO245-07-04	300	28.0	0.4	0.9	0.4	6.4	4.5	2.1	142512	39960	570	1226	627	9164	6442	3036
SO245_7-4	7	SO245-07-04	400								76865
SO245_7-4	7	SO245-07-04	500	18.4		0.6		5.2	4.9		55412	10196		338		2887	2737
SO245_7-4	7	SO245-07-04	600								2590
SO245_8-1	8	SO245-08-10	10								402312
SO245_8-1	8	SO245-08-10	20	38.8	7.1	6.0	1.6				397670	154177	28036	23980	6164
SO245_8-1	8	SO245-08-10	60	27.7	5.1	2.0	1.4	0.0	0.1	0.9	416101	115260	21055	8364	5992	166	291	3745
SO245_8-1	8	SO245-08-10	80								556361
SO245_8-1	8	SO245-08-10	100	23.2	8.9	1.4	0.9	0.2	0.5	0.8	495311	114863	43835	7083	4408	1139	2328	4012
SO245_8-1	8	SO245-08-10	125	15.4	13.2	1.2	0.8		0.7	0.5	506246	78063	66571	5822	4252		3341	2582
SO245_8-1	8	SO245-08-19	150	19.7	25.7	1.0	0.8	0.4	0.8	0.8	474507	93620	122138	4555	3938	1661	3701	3654
SO245_8-1	8	SO245-08-19	175	23.2	23.9	4.1	1.3	0.5	1.4	1.1	384750	89108	91955	15852	4809	2001	5271	4194
SO245_8-1	8	SO245-08-19	200	23.7	8.5	1.0	0.2	0.8	2.2	1.1	290739	68934	24713	2762	523	2239	6251	3198
SO245_8-1	8	SO245-08-19	225	32.9	3.3	0.3	0.2		3.6	0.6	175013	57562	5828	525	350		6353	998
SO245_8-1	8	SO245-08-19	250	29.2	2.2	0.4	0.2	3.2	4.3	1.7	136148	39741	2982	477	313	4343	5868	2328
SO245_8-1	8	SO245-08-10	275								120818
SO245_8-1	8	SO245-08-19	300	25.3	0.4	0.5	0.4		4.9	1.2	111986	28276	392	571	403		5431	1288
SO245_8-1	8	SO245-08-10	350								75907
SO245_8-1	8	SO245-08-10	400								66453
SO245_8-1	8	SO245-08-10	450								52501
SO245_8-1	8	SO245-08-05	500	21.0		0.6		5.2	4.9	1.7	46839	9850		290		2422	2281	792	insdc:ERS1883590
SO245_8-1	8	SO245-08-19	600								39137
SO245_8-1	8	SO245-08-19	750								71975
SO245_8-1	8	SO245-08-05	1000	19.0				5.1	5.5	1.4	71160	13485				3608	3907	1018
SO245_8-1	8	SO245-08-19	1250								15908
SO245_8-1	8	SO245-08-01	1500	14.0				5.9	4.5	1.2	13559	1894				800	613	159	insdc:ERS1883589
SO245_8-1	8	SO245-08-19	1750								11104
SO245_8-1	8	SO245-08-19	2000								10420
SO245_8-1	8	SO245-08-01	2250	14.6				9.9	3.6	1.5	9258	1351				916	335	142	insdc:ERS1883588
SO245_8-1	8	SO245-08-19	2800								8252
SO245_8-1	8	SO245-08-19	3000								8564
SO245_8-1	8	SO245-08-01	3660	14.3				5.6	6.4	1.4	9318	1328				522	600	130	insdc:ERS1883587
SO245_9-1	9	SO245-09-01	5								385825
SO245_9-1	9	SO245-09-01	25	34.7	8.3	4.4	1.7				451282	156595	37592	20037	7717				insdc:ERS1883586
SO245_9-1	9	SO245-09-01	50	34.7	8.0	2.3	1.3	0.1	0.1	1.0	477814	165658	38177	10942	6020	287	526	4778
SO245_9-1	9	SO245-09-01	100	28.9	19.9	1.1	1.3	0.5	0.6	1.2	500644	144536	99428	5607	6508	2453	2804	5908
SO245_9-1	9	SO245-09-01	150	26.9	24.9	2.2	0.7	0.3	0.8	1.6	486344	130729	120905	10748	3550	1410	4037	7927	insdc:ERS1883585
SO245_9-1	9	SO245-09-01	175	29.1	11.8	1.0	0.3	0.2	2.1	1.8	232125	67455	27460	2252	720	418	4944	4085	insdc:ERS1883584
SO245_9-1	9	SO245-09-01	200	32.1	2.9	1.1	0.4	1.2	3.6	0.7	164641	52899	4692	1762	593	2042	5845	1136	insdc:ERS1883583
SO245_9-1	9	SO245-09-01	225	28.3	1.3	1.0	0.4	2.5	3.9	2.2	133934	37863	1714	1299	576	3335	5237	2987	insdc:ERS1883582
SO245_9-1	9	SO245-09-01	250	24.4	0.3	0.8	0.6	2.5	4.1	1.0	81368	19862	269	635	480	2042	3312	830	insdc:ERS1883581
SO245_9-1	9	SO245-09-01	300	25.0	0.5	1.0	0.8	3.1	4.0	2.6	91479	22906	421	915	714	2854	3650	2360	insdc:ERS1883580
SO245_9-1	9	SO245-09-01	400								77316
SO245_9-1	9	SO245-09-01	500	21.5		1.3		1.6	4.1	1.6	76566	16485		1003		1248	3116	1202	insdc:ERS1883579
SO245_10-1	10	SO245-10-10	5								452347
SO245_10-1	10	SO245-10-10	10								461297
SO245_10-1	10	SO245-10-10	20	37.6	7.1	5.1	2.8				469331	176375	33369	23701	13235				insdc:ERS1883578
SO245_10-1	10	SO245-10-10	40								512628
SO245_10-1	10	SO245-10-10	60	29.9	12.6	2.0	1.4	0.1	0.1	1.1	515908	154411	64798	10215	7120	258	516	5727	insdc:ERS1883577
SO245_10-1	10	SO245-10-10	80								546740
SO245_10-1	10	SO245-10-10	100	24.1	26.2	1.0	1.1	0.2	0.3	1.2	551645	132726	144255	5627	5792	883	1600	6840	insdc:ERS1883576
SO245_10-1	10	SO245-10-10	125	35.5	27.2	3.4	1.2		0.4	1.6	604198	214611	164523	20301	7371		2417	9848	insdc:ERS1883575
SO245_10-1	10	SO245-10-06	150	27.3	16.5	1.6	0.9	0.4	1.5	2.4	345314	94374	56839	5421	3108	1347	5076	8288	insdc:ERS1883574
SO245_10-1	10	SO245-10-06	175	28.9	6.6	1.2	0.5	1.5	3.2	4.5	180380	52148	11905	2165	830	2778	5700	8117	insdc:ERS1883573
SO245_10-1	10	SO245-10-06	200	28.7	2.6	1.5	0.5	3.4	3.0	2.6	158830	45584	4066	2414	794	5416	4701	4098	insdc:ERS1883572
SO245_10-1	10	SO245-10-06	225	30.5	1.1	0.5	0.2	2.0	4.4	3.0	148211	45249	1571	726	326	3023	6551	4432
SO245_10-1	10	SO245-10-06	250	31.9	0.4	0.6	0.3	3.5	4.8	2.6	131739	42077	487	738	343	4637	6271	3478	insdc:ERS1883571
SO245_10-1	10	SO245-10-10	275								113016
SO245_10-1	10	SO245-10-06	300	23.9	0.4	1.0	1.0	2.8	4.6	3.9	92916	22225	400	957	929	2620	4237	3633	insdc:ERS1883570
SO245_10-1	10	SO245-10-10	350								62738
SO245_10-1	10	SO245-10-10	400								69819
SO245_10-1	10	SO245-10-03	500	24.9		0.7		3.8	4.9	2.9	65301	16253		444		2449	3200	1881	insdc:ERS1883569
SO245_10-1	10	SO245-10-06	600								52136
SO245_10-1	10	SO245-10-06	750								48855
SO245_10-1	10	SO245-10-03	1000	24.8				4.2	6.1	3.3	35125	8707				1458	2139	1163	insdc:ERS1883568
SO245_10-1	10	SO245-10-06	1250								33841
SO245_10-1	10	SO245-10-01	1500	21.6				2.1	5.8	3.2	28154	6070				586	1633	890	insdc:ERS1883567
SO245_10-1	10	SO245-10-06	1750								21867
SO245_10-1	10	SO245-10-01	2000	15.4				2.0	5.4	4.2	16910	2604				345	911	710	insdc:ERS1883566
SO245_10-1	10	SO245-10-03	2400								14168
SO245_10-1	10	SO245-10-03	2800	14.6				3.9	5.4	5.6	12366	1805				476	671	694
SO245_10-1	10	SO245-10-01	3200								12073
SO245_10-1	10	SO245-10-01	3600								11885
SO245_10-1	10	SO245-10-01	3930	15.3				4.9	5.1	4.1	13619	2086				671	700	557
SO245_11-1	11	SO245-11-01	5								700924
SO245_11-1	11	SO245-11-01	20		5.4	6.2	3.0				670255		35926	41690	19840
SO245_11-1	11	SO245-11-01	50	21.0	21.2	2.7	3.4	0.1	0.0	0.7	721548	151742	153185	19265	24388	866	72	5123
SO245_11-1	11	SO245-11-01	75	22.7	22.0	2.0	3.5		0.1	0.9	833200	189053	183554	16247	29079		417	7332
SO245_11-1	11	SO245-11-01	100	19.5	21.2	1.7	1.8	0.5	0.1	1.7	715056	139507	151234	12299	12585	3432	715	12442
SO245_11-1	11	SO245-11-01	125								374190
SO245_11-1	11	SO245-11-01	150	26.3	5.0	0.8	0.3	2.1	1.5	1.9	251205	65992	12560	1884	779	5200	3743	4698
SO245_11-1	11	SO245-11-01	175								222475
SO245_11-1	11	SO245-11-01	200	31.2	0.7	0.5	0.2	2.9	4.2	2.6	222046	69234	1643	1177	355	6484	9237	5818
SO245_11-1	11	SO245-11-01	300	33.5	0.1	0.9	0.4	2.4	4.9	2.8	141614	47469	142	1303	566	3370	6939	4008
SO245_11-1	11	SO245-11-01	400								113076
SO245_11-1	11	SO245-11-01	500	25.7		0.9		3.9	3.9	3.1	90287	23168		813		3503	3503	2835
SO245_12-2	12	SO245-12-14	5								742726
SO245_12-2	12	SO245-12-14	10								826145
SO245_12-2	12	SO245-12-14	20	42.1	1.2	4.8	5.1				746104	313811	9252	36111	38051				insdc:ERS1883563
SO245_12-2	12	SO245-12-14	40	35.6	6.4	3.6	5.0				915345	325497	58308	33044	45401				insdc:ERS1883562
SO245_12-2	12	SO245-12-14	60	43.5	5.3	1.3	3.1	0.1	0.0	0.5	777152	337906	41034	9714	24014	466	78	4197	insdc:ERS1883561
SO245_12-2	12	SO245-12-14	80	27.6	9.9	1.4	2.0		0.1	1.5	562666	155240	55479	7652	10972		619	8440	insdc:ERS1883560
SO245_12-2	12	SO245-12-14	100	40.0	11.2	1.5	1.6	0.4	0.5	1.5	377026	150659	42340	5768	5844	1320	1697	5768	insdc:ERS1883559
SO245_12-2	12	SO245-12-14	125								370016
SO245_12-2	12	SO245-12-10	150	35.7	3.8	1.1	0.5	0.5	2.6	1.4	325635	116122	12244	3419	1693	1596	8336	4591	insdc:ERS1883558
SO245_12-2	12	SO245-12-14	175								257210
SO245_12-2	12	SO245-12-10	200	29.0	0.6	1.4	0.4	2.0	4.7	2.7	233029	67625	1352	3332	816	4567	10906	6199	insdc:ERS1883557
SO245_12-2	12	SO245-12-14	225								164715
SO245_12-2	12	SO245-12-14	250								142023
SO245_12-2	12	SO245-12-14	275								74318
SO245_12-2	12	SO245-12-10	300	26.4	0.3	0.7	0.5	3.4	4.3	2.5	276698	73076	858	1882	1383	9297	11898	6890	insdc:ERS1883556
SO245_12-2	12	SO245-12-14	350								116053
SO245_12-2	12	SO245-12-14	400								108980
SO245_12-2	12	SO245-12-06	500	23.3		1.0		3.0	4.6	2.5	89400	20830		930		2691	4148	2208	insdc:ERS1883555
SO245_12-2	12	SO245-12-14	600								81270
SO245_12-2	12	SO245-12-14	750								86228
SO245_12-2	12	SO245-12-14	800								73320
SO245_12-2	12	SO245-12-06	1000	22.3				2.4	5.4	2.1	58758	13091				1422	3155	1234	insdc:ERS1883554
SO245_12-2	12	SO245-12-06	1250								48914
SO245_12-2	12	SO245-12-06	1500	25.2				2.5	6.2	1.4	32086	8073				805	1989	462
SO245_12-2	12	SO245-12-06	2000								24324
SO245_12-2	12	SO245-12-06	2500								20449
SO245_12-2	12	SO245-12-02	3000	17.6				4.6	5.6	2.7
SO245_12-2	12	SO245-12-06	4000								17022
SO245_12-2	12	SO245-12-02	4500								15756
SO245_12-2	12	SO245-12-02	5125	13.7				5.3	7.0	2.9	11108	1516				590	782	317
SO245_13-1	13	SO245-13-01	5								895647
SO245_13-1	13	SO245-13-01	20	30.2	2.9	1.3	4.0				957881	289184	27395	12548	37932
SO245_13-1	13	SO245-13-01	50	26.2	16.5	1.6	4.3	0.1	0.0	0.7	1262671	330189	207709	19824	54674	1768	379	8334
SO245_13-1	13	SO245-13-01	75	9.9	18.3	1.7	4.0		0.1	1.0	768478	75926	140478	12757	30739		384	7454
SO245_13-1	13	SO245-13-01	100	16.5	16.9	1.3	2.8	1.0	0.2	1.1	429963	70944	72836	5633	12039	4300	774	4515
SO245_13-1	13	SO245-13-01	125								327848
SO245_13-1	13	SO245-13-01	150	21.6	1.6	0.8	0.6	3.3	2.0	1.8	572761	123602	9221	4811	3150	18729	11512	10195
SO245_13-1	13	SO245-13-01	175	24.3	0.7	0.3	0.5	2.2	3.3	1.1	122615	29820	871	405	625	2710	3985	1361
SO245_13-1	13	SO245-13-01	200								217531
SO245_13-1	13	SO245-13-01	300	22.9	0.3	0.7	0.0	3.5	3.7	1.4	152379	34849	503	990	30	5318	5623	2149
SO245_13-1	13	SO245-13-01	400								119838
SO245_13-1	13	SO245-13-01	500	23.6		0.5		2.7	4.2	1.6	159404	37635		861		4320	6711	2614
SO245_14-2	14	SO245-14-04	5								1390562
SO245_14-2	14	SO245-14-04	10								1432935
SO245_14-2	14	SO245-14-04	20	38.2	1.7	2.0	5.5				1413753	540336	23892	28416	77191
SO245_14-2	14	SO245-14-04	40	39.3	3.5	2.5	2.5				1444922	568143	51006	35401	36557
SO245_14-2	14	SO245-14-04	60	29.3	11.6	0.5	2.0	0.3	0.1	1.0	866110	253684	100382	4677	17582	2598	779	8921
SO245_14-2	14	SO245-14-04	80	27.5	11.9	0.8	1.5		0.6	1.2	485289	133260	57895	3834	7182		2766	5969
SO245_14-2	14	SO245-14-04	100	41.2	8.2	1.0	1.1	0.7	1.3	0.8	336285	138651	27609	3363	3531	2421	4304	2690
SO245_14-2	14	SO245-14-04	125								282407
SO245_14-2	14	SO245-14-14	150	29.6	0.9	0.4	0.6	2.1	3.3	1.0	253502	74910	2282	938	1597	5197	8416	2459
SO245_14-2	14	SO245-14-04	175								237929
SO245_14-2	14	SO245-14-14	200	25.0	0.4	0.8	0.2	2.3	4.8	1.1	211205	52822	845	1669	317	4921	10053	2344
SO245_14-2	14	SO245-14-04	225								213490
SO245_14-2	14	SO245-14-04	250								184108
SO245_14-2	14	SO245-14-04	275								165366
SO245_14-2	14	SO245-14-14	300	23.5	0.3	0.7	0.0	2.2	4.9	1.8	164405	38668	543	1184	49	3584	8039	2976
SO245_14-2	14	SO245-14-04	350								135753
SO245_14-2	14	SO245-14-04	400								126023
SO245_14-2	14	SO245-14-06	500	22.3		0.7		2.8	3.7	2.0	104070	23218		728		2872	3861	2081
SO245_14-2	14	SO245-14-04	600								108251
SO245_14-2	14	SO245-14-04	750								83021
SO245_14-2	14	SO245-14-04	800								72874
SO245_14-2	14	SO245-14-06	1000	26.7				3.3	5.5	1.2	58461	15580				1923	3204	713
SO245_14-2	14	SO245-14-14	1250								45868
SO245_14-2	14	SO245-14-14	1500								30608
SO245_14-2	14	SO245-14-06	2000								22617
SO245_14-2	14	SO245-14-06	2500								16936
SO245_14-2	14	SO245-14-02	3000	12.6				6.2		0.8	14537	1832				898		122
SO245_14-2	14	SO245-14-02	3500								14406
SO245_14-2	14	SO245-14-02	4000								19214
SO245_14-2	14	SO245-14-02	4500								13391
SO245_14-2	14	SO245-14-02	5000								30894
SO245_15-13	15	SO245-15-11	5								924561
SO245_15-13	15	SO245-15-11	10								924561
SO245_15-13	15	SO245-15-11	20	30.6	8.0	2.3	5.4				917854	281047	73337	21111	49839
SO245_15-13	15	SO245-15-11	40	34.1	13.6	1.2	5.9				981632	334442	133207	11878	57524
SO245_15-13	15	SO245-15-11	60	35.3	5.4	0.9	3.6	0.6	0.1	0.7	1106770	390800	59987	9740	39622	6751	1328	7415
SO245_15-13	15	SO245-15-11	80								915770
SO245_15-13	15	SO245-15-11	100	34.1	0.2	0.7	1.6	0.9	1.9	0.5	467512	159281	1029	3226	7480	4348	8742	2431
SO245_15-13	15	SO245-15-11	125								318042
SO245_15-13	15	SO245-15-09	150	24.3	0.2	0.8	1.7	1.1	2.0	1.2	327990	79603	623	2558	5576	3739	6396	4001
SO245_15-13	15	SO245-15-11	175								268985
SO245_15-13	15	SO245-15-09	200	28.7	0.2	0.8	0.9	1.5	2.4	0.7	266511	76515	453	2079	2265	3971	6396	1946
SO245_15-13	15	SO245-15-11	225								257242
SO245_15-13	15	SO245-15-09	250	26.3	0.2	0.7	0.6	1.8	3.0	1.0	253387	66590	431	1698	1394	4637	7627	2610
SO245_15-13	15	SO245-15-11	275								232663
SO245_15-13	15	SO245-15-09	300	26.6	0.1	0.8	0.2	1.4	3.6	0.8	218219	57937	175	1746	524	3011	7856	1658
SO245_15-13	15	SO245-15-11	350								212456
SO245_15-13	15	SO245-15-09	400								192157
SO245_15-13	15	SO245-15-04	500	24.1		0.4		1.4	4.0	1.6	151083	36441		619		2040	6089	2463
SO245_15-13	15	SO245-15-09	600								126946
SO245_15-13	15	SO245-15-04	750	23.6				1.7	4.7	1.1	91798	21646				1524	4333	1019
SO245_15-13	15	SO245-15-04	910								87909
SO245_15-13	15	SO245-15-04	1000	23.1				1.9	4.7	1.2	73890	17076				1426	3502	916
SO245_15-13	15	SO245-15-04	1250	20.1				2.5	5.5	1.9	53993	10826				1339	2986	1010
SO245_15-13	15	SO245-15-04	1500								39704
SO245_15-13	15	SO245-15-04	2000								30940
SO245_15-13	15	SO245-15-13	2400								24585
SO245_15-13	15	SO245-15-13	2800	14.0				4.5	6.3	0.2
SO245_15-13	15	SO245-15-13	3200								19971
SO245_15-13	15	SO245-15-13	3600								17949
SO245_15-13	15	SO245-15-13	4000								17652
SO245_15-13	15	SO245-15-13	4600								25003
SO245_15-13