/* DATA DESCRIPTION: Citation: Engel, Friederike G; Lewandowska, Aleksandra M; Eggers, Sarah Lena; Matthiessen, Birte (2017): Manipulation experiment on phytoplankton diversity, species composition and biomass. PANGAEA, https://doi.org/10.1594/PANGAEA.881071, Supplement to: Engel, FG et al. (2017): Manipulation of Non-random Species Loss in Natural Phytoplankton: Qualitative and Quantitative Evaluation of Different Approaches. Frontiers in Marine Science, 4, https://doi.org/10.3389/fmars.2017.00317 Abstract: Ecological research in recent decades revealed that species loss has a predominantly negative effect on ecosystem functioning and stability. Most of these studies were based on random species loss scenarios, but extinctions in nature are not random. Recent experimental studies using macroscopic communities largely advanced knowledge about the effects of non-random species loss. However, in microscopic communities like the phytoplankton, implementing realistic species loss scenarios is challenging and experimental data are scarce. Creating more realistic experiments to study the role of phytoplankton diversity for ecosystem functioning is particularly important, as they provide up to 50% of global primary productivity, form the basis of all pelagic food webs, and are important for biogeochemical cycling. In this study, we experimentally tested and evaluated three methods for non-random species loss in a natural marine phytoplankton community. Dilution, filtration, and heat stress removed the targeted rare, large, and sensitive species, respectively. All these species groups are extremely vulnerable to extinction in future climate scenarios and play important roles in the communities. Dilution and filtration with a fine mesh additionally decreased initial biomass, which increased the variability of species left in the respective replicates. The methods tested in this study can be used to non-randomly manipulate phytoplankton species diversity in communities used for experiments. However, in studies where species identities are more important than species richness, the dilution and filtration methods should be modified to eliminate the effect of decreasing initial biomass. Parameter(s): Sampling (Sampling) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Treatment (Treat) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Replicate (Repl) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Identification (ID) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Treatment (Treat) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) * COMMENT: Dilution: 0=no dilution, 1=weak dilution, 2=strong dilution Treatment (Treat) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) * COMMENT: Filtration: 0=no filtration, 1=weak filtration, 2=strong filtration Treatment (Treat) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) * COMMENT: Stress: yes=heat stress applied, no=no heat stress applied Cell [#/ml] (Cell) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Biovolume [mm**3/l] (Biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Species richness (S) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Shannon Diversity Index (H') * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Pielou evenness index (J') * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Dissimilarity index (DISS) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) * COMMENT: within treatment variation calculated from dissimilarites based on Bray-Curtis dissimilarity matrix Skeletonema costatum, biovolume [µm**3/ml] (S. costatum biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Detonula confervacea, biovolume [µm**3/ml] (D. confervacea biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Chaetoceros spp., biovolume [µm**3/ml] (Chaetoceros spp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Thalassiosira spp., biovolume [µm**3/ml] (Thalassiosira spp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Heterocapsa rotundata, biovolume [µm**3/ml] (H. rotundata biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Plagioselmis sp., biovolume [µm**3/ml] (Plagioselmis sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Thalassionema nitzschioides, biovolume [µm**3/ml] (T. nitzschioides biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Thalassiosira rotula, biovolume [µm**3/ml] (T. rotula biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Nitzschia microcephala, biovolume [µm**3/ml] (N. microcephala biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Fragilaria sp., biovolume [µm**3/ml] (Fragilaria sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Asterionella formosa, biovolume [µm**3/ml] (A. formosa biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Ceratium fusus, biovolume [µm**3/ml] (C. fusus biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Eutreptiella sp., biovolume [µm**3/ml] (Eutreptiella sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Pseudo-nitzschia pungens, biovolume [µm**3/ml] (P-n pungens biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Ditylum brightwellii, biovolume [µm**3/ml] (D. brightwellii biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Navicula spp., biovolume [µm**3/ml] (Navicula spp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Cylindrotheca closterium, biovolume [µm**3/ml] (C. closterium biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Gymnodinium sp., biovolume [µm**3/ml] (Gymnodinium sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Dictyocha speculum, biovolume [µm**3/ml] (D. speculum biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Scenedesmus sp., biovolume [µm**3/ml] (Scenedesmus sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Teleaulax sp., biovolume [µm**3/ml] (Teleaulax sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Dinobryon divergens, biovolume [µm**3/ml] (D. divergens biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Gyrodinium sp., biovolume [µm**3/ml] (Gyrodinium sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Tetraedron minimum, biovolume [µm**3/ml] (T. minimum biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Snowella sp., biovolume [µm**3/ml] (Snowella sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Attheya decora, biovolume [µm**3/ml] (A. decora biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Dinobryon faculiferum, biovolume [µm**3/ml] (D. faculiferum biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Apedinella radians, biovolume [µm**3/ml] (A. radians biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Pseudopedinella sp., biovolume [µm**3/ml] (Pseudopedinella sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Licmophora sp., biovolume [µm**3/ml] (Licmophora sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Ebria tripartita, biovolume [µm**3/ml] (E. tripartita biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Diatomaceae centric, biovolume [µm**3/ml] (Diatoma centric biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Coelastrum sp., biovolume [µm**3/ml] (Coelastrum sp. biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) Brockmanniella brockmannii, biovolume [µm**3/ml] (B. brockmannii biovol) * PI: Engel, Friederike G (https://orcid.org/0000-0002-6566-8592, fg.engel@ufl.edu) License: Creative Commons Attribution 3.0 Unported (CC-BY-3.0) (URI: https://creativecommons.org/licenses/by/3.0/) Size: 1858 data points */ Sampling Treat Repl ID Treat (Dilution: 0=no dilution, 1=we...) Treat (Filtration: 0=no filtration, ...) Treat (Stress: yes=heat stress appli...) Cell [#/ml] Biovol [mm**3/l] S H' J' DISS (within treatment variation ca...) S. costatum biovol [µm**3/ml] D. confervacea biovol [µm**3/ml] Chaetoceros spp. biovol [µm**3/ml] Thalassiosira spp. biovol [µm**3/ml] H. rotundata biovol [µm**3/ml] Plagioselmis sp. biovol [µm**3/ml] T. nitzschioides biovol [µm**3/ml] T. rotula biovol [µm**3/ml] N. microcephala biovol [µm**3/ml] Fragilaria sp. biovol [µm**3/ml] A. formosa biovol [µm**3/ml] C. fusus biovol [µm**3/ml] Eutreptiella sp. biovol [µm**3/ml] P-n pungens biovol [µm**3/ml] D. brightwellii biovol [µm**3/ml] Navicula spp. biovol [µm**3/ml] C. closterium biovol [µm**3/ml] Gymnodinium sp. biovol [µm**3/ml] D. speculum biovol [µm**3/ml] Scenedesmus sp. biovol [µm**3/ml] Teleaulax sp. biovol [µm**3/ml] D. divergens biovol [µm**3/ml] Gyrodinium sp. biovol [µm**3/ml] T. minimum biovol [µm**3/ml] Snowella sp. biovol [µm**3/ml] A. decora biovol [µm**3/ml] D. faculiferum biovol [µm**3/ml] A. radians biovol [µm**3/ml] Pseudopedinella sp. biovol [µm**3/ml] Licmophora sp. biovol [µm**3/ml] E. tripartita biovol [µm**3/ml] Diatoma centric biovol [µm**3/ml] Coelastrum sp. biovol [µm**3/ml] B. brockmannii biovol [µm**3/ml] peak sensitive 2 S.2 0 0 yes 106631.20 15.87 13 0.31 0.12 0.25 14956973.16 17518.11 391965.16 259971.36 2201.44 0.00 14616.97 0.00 27647.36 0.00 0.00 0.00 0.00 0.00 0.00 215.89 280.86 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 4990.07 114175.21 72219.15 0.00 10894.31 0.00 0.00 0.00 peak sensitive 10 S.10 0 0 yes 95000.85 10.09 11 0.60 0.25 0.18 8541711.15 16423.23 882693.20 449950.44 9631.31 0.00 97446.48 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2806.58 561.72 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 74527.55 9642.45 0.00 5447.15 0.00 0.00 0.00 peak sensitive 22 S.22 0 0 yes 77708.24 8.61 13 0.45 0.17 0.20 7792475.11 45620.07 481469.02 40495.54 8943.36 918.62 18271.22 0.00 1382.94 0.00 0.00 0.00 0.00 0.00 0.00 179.91 702.15 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 88078.02 102138.51 0.00 27235.77 0.00 0.00 0.00 peak sensitive 30 S.30 0 0 yes 92366.39 11.57 13 0.28 0.11 0.18 11028980.42 31477.85 41974.22 184479.68 11832.75 0.00 51159.40 0.00 4528.27 0.00 0.00 0.00 0.00 0.00 0.00 647.67 561.72 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 296.11 94853.25 95353.09 0.00 27235.77 0.00 0.00 0.00 peak weak filtration 4 F1.4 0 1 no 79537.57 11.11 12 1.00 0.40 0.24 8126182.93 1002487.61 731462.55 670426.15 12383.11 2755.85 15226.01 424787.84 5742.14 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 42383.62 0.00 0.00 5356.92 0.00 68089.43 0.00 0.00 0.00 peak weak filtration 7 F1.7 0 1 no 70566.17 9.05 12 1.18 0.47 0.17 5710766.76 1122187.62 1243794.96 544440.03 10089.94 459.31 24361.62 318590.88 1435.54 0.00 0.00 0.00 0.00 0.00 0.00 134.93 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 54201.86 0.00 0.00 18157.18 0.00 0.00 0.00 peak weak filtration 15 F1.15 0 1 no 73543.34 8.11 13 1.25 0.49 0.17 4718706.24 837900.09 1536997.25 494945.48 11007.21 1102.34 10962.73 467266.62 862.67 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 296.11 6775.23 3214.15 0.00 21788.62 0.00 0.00 0.00 peak weak filtration 26 F1.26 0 1 no 48448.42 5.87 12 0.87 0.35 0.23 4498538.19 613462.57 80244.84 238473.73 11557.57 826.76 12180.81 382309.05 14540.64 0.00 0.00 0.00 0.00 0.00 0.00 215.89 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 740.28 0.00 0.00 0.00 21788.62 0.00 0.00 0.00 peak strong filtration 8 F2.8 0 2 no 91204.30 9.13 13 0.41 0.16 0.36 8301856.43 289005.36 6172.68 454449.94 6053.97 3582.61 7308.49 0.00 5742.14 0.00 0.00 0.00 0.00 0.00 0.00 1079.45 4212.89 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 13550.46 33212.87 0.00 5447.15 0.00 0.00 0.00 peak strong filtration 19 F2.19 0 2 no 26575.88 1.94 11 1.12 0.47 0.59 1244245.56 233552.48 0.00 328463.82 39901.14 3031.44 0.00 0.00 189.35 0.00 0.00 0.00 0.00 0.00 0.00 215.89 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 148.06 44039.01 41783.94 0.00 5447.15 0.00 0.00 0.00 peak strong filtration 24 F2.24 0 2 no 54396.51 7.17 10 0.61 0.27 0.29 6140861.05 204093.53 0.00 431005.15 31645.73 2755.85 0.00 0.00 1640.61 0.00 0.00 0.00 0.00 0.00 0.00 0.00 200.61 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 337.67 139070.55 215028.45 0.00 0.00 0.00 0.00 0.00 peak strong filtration 32 F2.32 0 2 no 70740.77 5.64 12 0.63 0.25 0.31 4809870.70 105552.10 22633.16 440951.43 7796.77 0.00 6090.41 0.00 7848.43 0.00 0.00 0.00 0.00 0.00 0.00 0.00 468.10 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 296.11 81302.79 134994.26 0.00 18157.18 0.00 0.00 0.00 peak weak dilution 5 D1.5 1 0 no 59066.41 10.79 11 1.34 0.56 0.37 4271879.13 504031.51 1348730.52 733419.21 0.00 344.48 5329.10 3909725.02 0.00 0.00 0.00 0.00 0.00 0.00 0.00 4212.89 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2220.85 0.00 5356.92 0.00 6808.94 0.00 0.00 0.00 peak weak dilution 9 D1.9 1 0 no 76892.80 12.31 13 0.78 0.30 0.32 10024002.68 413686.24 682081.11 688424.17 2476.62 1653.51 1218.08 264818.17 0.00 0.00 0.00 0.00 0.00 0.00 0.00 842.58 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1036.40 138892.26 86782.02 0.00 5447.15 0.00 0.00 0.00 peak weak dilution 20 D1.20 1 0 no 67783.82 9.05 11 1.41 0.59 0.34 4240038.23 637171.91 1981430.19 611932.59 825.54 551.17 0.00 1486757.43 21507.86 0.00 0.00 0.00 0.00 0.00 0.00 4317.81 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1184.46 0.00 62140.22 0.00 0.00 0.00 0.00 0.00 peak weak dilution 23 D1.23 1 0 no 97878.20 10.07 13 0.55 0.22 0.35 8855939.41 353229.63 35801.54 403884.08 550.36 275.59 15835.05 233633.31 2296.86 0.00 0.00 0.00 0.00 0.00 0.00 2158.90 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2749.63 0.00 152544.54 0.00 10894.31 0.00 0.00 0.00 peak strong dilution 1 D2.1 2 0 no 52739.58 4.72 10 1.19 0.52 0.67 2048781.55 20321.92 1253053.98 1295857.26 0.00 0.00 8526.57 0.00 6890.57 0.00 0.00 0.00 0.00 0.00 0.00 431.78 280.86 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1332.51 0.00 88924.79 0.00 0.00 0.00 0.00 0.00 peak strong dilution 14 D2.14 2 0 no 111020.57 18.25 9 0.34 0.15 0.57 16913822.60 791666.22 138885.29 364459.85 275.18 0.00 26797.78 0.00 6675.22 0.00 0.00 0.00 0.00 0.00 0.00 0.00 280.86 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3997.54 0.00 0.00 0.00 0.00 0.00 0.00 0.00 peak strong dilution 27 D2.27 2 0 no 62747.32 12.69 9 1.24 0.56 0.55 4752039.89 1535329.39 30863.40 1268860.23 171.99 0.00 0.00 5097454.05 0.00 0.00 0.00 0.00 0.00 0.00 0.00 175.54 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2961.14 0.00 6428.30 0.00 0.00 0.00 0.00 0.00 peak strong dilution 29 D2.29 2 0 no 163441.42 10.30 8 0.60 0.29 0.46 8512524.50 1040205.82 1928.96 714921.25 0.00 0.00 0.00 0.00 15442.68 0.00 0.00 0.00 0.00 0.00 0.00 0.00 175.54 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1069.30 0.00 15475.53 0.00 0.00 0.00 0.00 0.00 peak weak dilution of heat stressed 3 SxD1.3 1 0 yes 91999.89 12.64 11 0.54 0.22 0.12 10722871.80 10891.49 1549171.15 129985.68 332.57 0.00 0.00 0.00 2296.86 0.00 0.00 0.00 0.00 0.00 0.00 215.89 280.86 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 3207.90 0.00 123804.26 0.00 98351.40 0.00 0.00 0.00 peak weak dilution of heat stressed 6 SxD1.6 1 0 yes 121678.25 14.11 9 0.66 0.30 0.15 10584629.52 15965.73 3159640.43 292467.78 230.95 0.00 8120.54 0.00 0.00 0.00 0.00 0.00 0.00 0.00 359.82 7606.61 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 40623.27 0.00 0.00 0.00 0.00 0.00 peak weak dilution of heat stressed 13 SxD1.13 1 0 yes 111770.33 10.86 10 0.76 0.33 0.14 8318464.18 11317.23 1727174.57 467948.45 55.43 275.59 8526.57 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 733.23 318758.54 10611.79 0.00 0.00 0.00 0.00 0.00 peak weak dilution of heat stressed 17 SxD1.17 1 0 yes 90128.91 9.52 12 0.41 0.16 0.15 8591708.34 12529.09 668706.97 159527.88 886.86 551.17 3654.24 0.00 2208.64 0.00 0.00 0.00 0.00 0.00 0.00 0.00 5532.08 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2916.27 0.00 67529.60 0.00 5447.15 0.00 0.00 0.00 peak strong dilution of heat stressed 12 SxD2.12 2 0 yes 136207.80 23.33 9 0.24 0.11 0.60 22202715.90 64291.70 745709.59 307238.88 0.00 0.00 0.00 0.00 3756.73 0.00 0.00 0.00 0.00 0.00 0.00 0.00 351.07 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1555.35 0.00 5627.47 0.00 3404.47 0.00 0.00 0.00 peak strong dilution of heat stressed 21 SxD2.21 2 0 yes 83373.05 8.96 6 0.89 0.50 0.56 2893056.28 0.00 5499857.63 386957.38 0.00 0.00 0.00 0.00 56886.90 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2072.80 0.00 122137.66 0.00 0.00 0.00 0.00 0.00 peak strong dilution of heat stressed 25 SxD2.25 2 0 yes 132568.44 15.94 7 0.77 0.39 0.41 8178487.63 0.00 7510635.12 246288.66 0.00 0.00 0.00 0.00 2511.09 0.00 0.00 0.00 0.00 0.00 0.00 0.00 351.07 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1013.02 0.00 4887.01 0.00 0.00 0.00 0.00 0.00 peak strong dilution of heat stressed 31 SxD2.31 2 0 yes 109682.57 8.40 8 0.57 0.27 0.50 7218058.61 5736.46 69442.65 253472.08 137.59 0.00 0.00 0.00 648945.33 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2566.32 0.00 204277.03 0.00 0.00 0.00 0.00 0.00 peak control 11 Co.11 0 0 no 57964.99 8.47 14 0.97 0.37 0.24 6259045.66 666760.58 543195.82 418453.91 7705.05 275.59 24361.62 509745.41 4554.01 0.00 0.00 0.00 0.00 0.00 0.00 215.89 280.86 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 30488.54 2142.77 0.00 5447.15 0.00 0.00 0.00 peak control 16 Co.16 0 0 no 61010.48 6.83 12 1.29 0.52 0.22 3963036.82 712740.31 1259226.66 503944.49 21739.24 551.17 25579.70 254872.70 1148.43 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 888.34 0.00 51426.39 0.00 38130.08 0.00 0.00 0.00 peak control 18 Co.18 0 0 no 40415.09 4.76 16 1.34 0.48 0.31 2436846.09 1109709.07 506159.74 179980.17 3095.78 1377.93 10658.21 477886.32 3588.84 0.00 0.00 0.00 0.00 0.00 0.00 539.73 175.54 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 2119.18 444.17 10162.85 2142.77 0.00 10213.42 0.00 0.00 0.00 peak control 28 Co.28 0 0 no 50165.80 6.80 11 1.04 0.43 0.19 4804113.58 763451.28 419742.22 472447.96 4402.88 551.17 23143.54 276112.09 1157.08 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 22499.04 0.00 16341.46 0.00 0.00 0.00 initial control Co 0 0 no 14005.00 2.63 22 1.72 0.56 339905.51 471482.27 56339.76 1255233.66 47449.26 51269.65 41226.16 14469.96 2256.90 4045.38 65208.00 0.00 0.00 0.00 0.00 1838.50 1103.89 1480.94 4186.00 2034.52 339.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 106650.00 19030.70 2363.32 875.50 141295.09 initial sensitive S 0 0 yes 14548.00 2.24 20 1.63 0.54 782693.43 201827.50 44883.11 828634.99 63384.15 44349.45 39896.28 166404.50 1504.60 2696.92 32604.00 0.00 5012.61 0.00 0.00 1602.79 919.91 3317.30 2093.00 5425.38 5254.50 0.00 296.19 0.00 0.00 0.00 0.00 0.00 0.00 0.00 4757.67 0.00 0.00 0.00 initial weak filtration F1 0 1 no 12275.00 2.22 20 1.53 0.51 288183.20 448085.40 48117.93 1145980.38 50351.90 31291.34 13564.74 65114.80 3510.73 6742.31 42042.00 0.00 0.00 0.00 0.00 188.56 797.26 1362.46 2093.00 678.17 339.00 0.00 59.24 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1189.42 0.00 0.00 69753.27 initial strong filtration F2 0 2 no 4979.00 0.85 20 1.50 0.50 74772.46 56010.68 943.49 488299.25 48219.35 39896.45 2393.78 7234.98 3009.19 1348.46 6864.00 0.00 0.00 0.00 0.00 565.69 1042.57 1895.60 4186.00 0.00 169.50 0.00 118.48 0.00 0.00 0.00 0.00 0.00 0.00 106650.00 2378.84 0.00 1167.33 0.00 initial weak dilution D1 1 0 no 1472.00 0.33 16 1.56 0.56 30246.31 62155.31 2021.76 164076.41 177.71 1323.86 3989.63 0.00 1504.60 0.00 24882.00 0.00 0.00 0.00 0.00 94.28 122.65 59.24 2093.00 678.17 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 0.00 26662.50 8325.93 0.00 0.00 0.00 initial strong dilution D2 2 0 no 229.00 0.10 16 1.67 0.60 2136.36 17252.23 269.57 20435.86 533.14 120.35 2127.80 7234.98 0.00 0.00 0.00 39000.00 0.00 4532.00 0.00 94.28 61.33 0.00 0.00 0.00 0.00 209.00 59.24 0.00 0.00 0.00 0.00 0.00 0.00 0.00 1189.42 0.00 291.83 0.00 initial weak dilution of heat stressed SxD1 1 0 yes 2658.00 0.45 19 1.75 0.59 116600.07 31432.15 12804.49 182350.79 11906.74 12095.30 5851.45 7234.98 1755.36 0.00 12012.00 0.00 0.00 0.00 46603.25 424.27 61.33 59.24 0.00 0.00 169.50 0.00 59.24 1105.50 0.00 0.00 0.00 0.00 0.00 0.00 3568.26 0.00 291.83 0.00 initial strong dilution of heat stressed SxD2 2 0 yes 215.00 0.06 13 1.54 0.60 13267.90 6380.96 1482.63 8056.45 355.43 0.00 1329.88 0.00 250.77 0.00 0.00 0.00 0.00 0.00 0.00 47.14 61.33 0.00 0.00 0.00 0.00 0.00 59.24 0.00 10.43 0.00 0.00 0.00 0.00 26662.50 1189.42 0.00 0.00 0.00