Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 Puga-Freitas Ruben 0000-0002-8565-2475 Ellison Aaron M 0000-0003-4151-6081 Blouin Manuel 0000-0002-3928-9070 Scheu Stefan sscheu@gwdg.de 0000-0003-4350-9520 Girin Thomas 0000-0002-5738-7731 Freschet Grégoire T Rose Laura 0000-0003-4523-4145 Scherer-Lorenzen Michael 0000-0001-9566-590X Barot Sebastien Lata Jean-Christophe Cesarz Simone 0000-0003-2334-5119 Eisenhauer Nico nico.eisenhauer@uni-jena.de 0000-0002-0371-6720 Gigon Agnès Weigelt Alexandra alexandra.weigelt@uni-leipzig.de 0000-0001-6242-603X Hansart Amandine Greiner Anna Pando Anne Gessler Arthur arthur.gessler@wsl.ch 0000-0002-1910-9589 Grignani Carlo Assandri Davide 0000-0002-3369-5254 Gleixner Gerd 0000-0002-4616-0953 LeGalliard Jean-Francois Urban-Mead Katherine Zavattaro Laura 0000-0001-8199-7399 Müller Marina E H Lange Markus 0000-0002-2802-9177 Lukac Martin 0000-0002-8535-6334 Bonkowski Michael Mannerheim Neringa Buchmann Nina nina.buchmann@usys.ethz.ch 0000-0003-0826-2980 Butenschoen Olaf 0000-0003-1866-9805 Rotter Paula Seyhun Rahme Devidal Sébastien Kayler Zachary 0000-0003-0090-7537 Roy Jacques 0000-0003-2275-9870 2017 Data from 14 labortories testing the impact of introduced variability on the reproducibility of a microcosm ecological experiment dataset https://doi.org/10.1594/PANGAEA.880980 2017-09-26T07:22:14 Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 Puga-Freitas Ruben 0000-0002-8565-2475 Ellison Aaron M 0000-0003-4151-6081 Blouin Manuel 0000-0002-3928-9070 Scheu Stefan sscheu@gwdg.de 0000-0003-4350-9520 Girin Thomas 0000-0002-5738-7731 Freschet Grégoire T Rose Laura 0000-0003-4523-4145 Scherer-Lorenzen Michael 0000-0001-9566-590X Barot Sebastien Lata Jean-Christophe Cesarz Simone 0000-0003-2334-5119 Eisenhauer Nico nico.eisenhauer@uni-jena.de 0000-0002-0371-6720 Gigon Agnès Weigelt Alexandra alexandra.weigelt@uni-leipzig.de 0000-0001-6242-603X Hansart Amandine Greiner Anna Pando Anne Gessler Arthur arthur.gessler@wsl.ch 0000-0002-1910-9589 Grignani Carlo Assandri Davide 0000-0002-3369-5254 Gleixner Gerd 0000-0002-4616-0953 LeGalliard Jean-Francois Urban-Mead Katherine Zavattaro Laura 0000-0001-8199-7399 Müller Marina E H Lange Markus 0000-0002-2802-9177 Lukac Martin 0000-0002-8535-6334 Bonkowski Michael Mannerheim Neringa Buchmann Nina nina.buchmann@usys.ethz.ch 0000-0003-0826-2980 Butenschoen Olaf 0000-0003-1866-9805 Rotter Paula Seyhun Rahme Devidal Sébastien Kayler Zachary 0000-0003-0090-7537 Roy Jacques 0000-0003-2275-9870 2018 Genotypic variability enhances the reproducibility of an ecological study journal article Nature Ecology & Evolution 2 https://doi.org/10.1038/s41559-017-0434-x 279-287 Although microcosm experiments are a frequent tool used to address fundamental ecological questions, there has been no quantitative assessment of the reproducibility of any microcosm experiment. This dataset contains the response variables measured in a multi-laboratory microcosm study in which the same microcosm experiment was repeated in 14 laboratories across Europe. All laboratories simultaneously run a simple microcosm experiment using grass (Brachypodium distachyon L.) monocultures and grass and legume (Medicago truncatula Gaertn.) mixtures. All twelve variables were then used to calculate the effect of the presence of nitrogen-fixing legume on the grass-legume mixtures (i.e. the net legume effect). The project tested a controversial hypotheses postulating that stringent levels of environmental and biotic standardization in experimental studies reduces reproducibility by amplifying impacts of lab-specific environmental factors not accounted for in the experimental design. This implies that the deliberate introduction of controlled systematic variability (CSV) in experimental designs can increase reproducibility. To test this hypothesis, each laboratory followed the same experimental protocol and introduced environmental and genotypic controlled systematic variability (CSV) within and among replicated microcosms established in either growth chambers (with stringent control of environmental conditions) or glasshouses (with more variable environmental conditions). Data were used to test the extent to which the effect size of the net legume effect varied with the CSV treatment and to estimate the number of laboratories that produced results that can be considered reproducible. not specified 18824 CC-BY-NC-ND-3.0 Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported https://creativecommons.org/licenses/by-nc-nd/3.0/ Laboratory Lab Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 Lab Method comment Method comm Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 type of setup Method comm (type of setup) Identification ID Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 Microcosm ID ID (Microcosm ID) Block Block Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 Block Treatment Treat treatment Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 legume Treat (legume) Treatment Treat treatment Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 controlled systematic variabiliy Treat (controlled systematic variabiliy) Genotype Genotype genotype Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 Genotype Biomass, dry mass Biom dm g biomass dry mass https://en.wikipedia.org/wiki/Dry_matter Mass M http://dbpedia.org/resource/Mass Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 seed Biom dm [g] (seed) Biomass, dry mass Biom dm g biomass dry mass https://en.wikipedia.org/wiki/Dry_matter Mass M http://dbpedia.org/resource/Mass Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 shoot Biom dm [g] (shoot) Biomass, dry mass Biom dm g biomass dry mass https://en.wikipedia.org/wiki/Dry_matter Mass M http://dbpedia.org/resource/Mass Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 root Biom dm [g] (root) Biomass, dry mass Biom dm g biomass dry mass https://en.wikipedia.org/wiki/Dry_matter Mass M http://dbpedia.org/resource/Mass Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 total Biom dm [g] (total) Root/shoot ratio Root/shoot shoot ratio Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 biomass Root/shoot (biomass) Brachypodium distachyon, height B. distachyon height cm https://en.wikipedia.org/wiki/Brachypodium_distachyon Brachypodium distachyon https://www.itis.gov/servlet/SingleRpt/SingleRpt?search_topic=TSN&search_value=41330 height http://purl.obolibrary.org/obo/PATO_0000119 Length L http://dbpedia.org/resource/Length Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 B. distachyon height [cm] Nitrogen, shoot N shoot % nitrogen N https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:25555 shoot Dimensionless Ratio ψ Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 N shoot [%] Carbon, shoot C shoot % carbon C https://www.ebi.ac.uk/chebi/searchId.do?chebiId=CHEBI:27594 shoot Dimensionless Ratio ψ Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 C shoot [%] δ15N δ15N ‰ air δ15N http://en.wikipedia.org/wiki/List_of_elements_by_stability_of_isotopes Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 δ15N [‰ air] δ13C δ13C ‰ PDB δ13C http://en.wikipedia.org/wiki/List_of_elements_by_stability_of_isotopes Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 δ13C [‰ PDB] Evapotranspiration ET ml/day evapotranspiration ET https://en.wikipedia.org/wiki/Evapotranspiration Volume per Unit Time Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 ET [ml/day] Litter mass Litter m g Mass M http://dbpedia.org/resource/Mass Milcu Alexandru alex.milcu@cnrs.fr 0000-0002-2889-1234 mass remaining Litter m [g] (mass remaining) supplement citable author47035 author53265 author53271 author53990 author55387 author55396 author55397 author55402 author58448 author62348 author62353 author63071 author63072 author63073 author63074 author63075 author63076 author63077 author63078 author63079 author63080 author63081 author63082 author63083 author63084 author63085 author63086 author63087 author63088 author63089 author63091 author63092 author63093 author63094 author63095 author63096 author63097 journal16858 license106 param142457 param144116 param150677 param15427 param159771 param159772 param159773 param159774 param159775 param17863 param20622 param20813 param45621 param7056 param96278 pi62348 ref92926 term1073089 term1073092 term1073288 term1207639 term2663825 term38477 term38548 term38564 term40438 term40594 term40915 term41013 term41165 term43794 term43863 term43892 term43985 topotype1