de.pangaea.dataset880980 und dataset PANGAEA

info@pangaea.de

https://www.pangaea.de/ information pointOfContact 2026-04-30T22:38:00Z https://doi.pangaea.de/10.1594/PANGAEA.880980 2017-09-26T07:22:14 publication https://doi.org/10.1594/PANGAEA.880980 Milcu, Alexandru

alex.milcu@cnrs.fr

https://orcid.org/0000-0002-2889-1234 information author Puga-Freitas, Ruben https://orcid.org/0000-0002-8565-2475 information author Ellison, Aaron M https://orcid.org/0000-0003-4151-6081 information author Blouin, Manuel https://orcid.org/0000-0002-3928-9070 information author Scheu, Stefan

sscheu@gwdg.de

https://orcid.org/0000-0003-4350-9520 information author Girin, Thomas https://orcid.org/0000-0002-5738-7731 information author Freschet, Grégoire T author Rose, Laura https://orcid.org/0000-0003-4523-4145 information author Scherer-Lorenzen, Michael https://orcid.org/0000-0001-9566-590X information author Barot, Sebastien author Lata, Jean-Christophe author Cesarz, Simone https://orcid.org/0000-0003-2334-5119 information author Eisenhauer, Nico

nico.eisenhauer@uni-jena.de

https://orcid.org/0000-0002-0371-6720 information author Gigon, Agnès author Weigelt, Alexandra

alexandra.weigelt@uni-leipzig.de

https://orcid.org/0000-0001-6242-603X information author Hansart, Amandine author Greiner, Anna author Pando, Anne author Gessler, Arthur

arthur.gessler@wsl.ch

https://orcid.org/0000-0002-1910-9589 information author Grignani, Carlo author Assandri, Davide https://orcid.org/0000-0002-3369-5254 information author Gleixner, Gerd https://orcid.org/0000-0002-4616-0953 information author LeGalliard, Jean-Francois author Urban-Mead, Katherine author Zavattaro, Laura https://orcid.org/0000-0001-8199-7399 information author Müller, Marina E H author Lange, Markus https://orcid.org/0000-0002-2802-9177 information author Lukac, Martin https://orcid.org/0000-0002-8535-6334 information author Bonkowski, Michael author Mannerheim, Neringa author Buchmann, Nina

nina.buchmann@usys.ethz.ch

https://orcid.org/0000-0003-0826-2980 information author Butenschoen, Olaf https://orcid.org/0000-0003-1866-9805 information author Rotter, Paula author Seyhun, Rahme author Devidal, Sébastien author Kayler, Zachary https://orcid.org/0000-0003-0090-7537 information author Roy, Jacques https://orcid.org/0000-0003-2275-9870 information author PANGAEA

info@pangaea.de

https://www.pangaea.de/ information publisher tableDigital Supplement to: Milcu, Alexandru; Puga-Freitas, Ruben; Ellison, Aaron M; Blouin, Manuel; Scheu, Stefan; Girin, Thomas; Freschet, Grégoire T; Rose, Laura; Scherer-Lorenzen, Michael; Barot, Sebastien; Lata, Jean-Christophe; Cesarz, Simone; Eisenhauer, Nico; Gigon, Agnès; Weigelt, Alexandra; Hansart, Amandine; Greiner, Anna; Pando, Anne; Gessler, Arthur; Grignani, Carlo; Assandri, Davide; Gleixner, Gerd; LeGalliard, Jean-Francois; Urban-Mead, Katherine; Zavattaro, Laura; Müller, Marina E H; Lange, Markus; Lukac, Martin; Bonkowski, Michael; Mannerheim, Neringa; Buchmann, Nina; Butenschoen, Olaf; Rotter, Paula; Seyhun, Rahme; Devidal, Sébastien; Kayler, Zachary; Roy, Jacques (2018): Genotypic variability enhances the reproducibility of an ecological study. Nature Ecology & Evolution, 2, 279-287, https://doi.org/10.1038/s41559-017-0434-x Although microcosm experiments are a frequent tool used to address fundamental ecological questions, there has been no quantitative assessment of the reproducibility of any microcosm experiment. This dataset contains the response variables measured in a multi-laboratory microcosm study in which the same microcosm experiment was repeated in 14 laboratories across Europe. All laboratories simultaneously run a simple microcosm experiment using grass (Brachypodium distachyon L.) monocultures and grass and legume (Medicago truncatula Gaertn.) mixtures. All twelve variables were then used to calculate the effect of the presence of nitrogen-fixing legume on the grass-legume mixtures (i.e. the net legume effect). The project tested a controversial hypotheses postulating that stringent levels of environmental and biotic standardization in experimental studies reduces reproducibility by amplifying impacts of lab-specific environmental factors not accounted for in the experimental design. This implies that the deliberate introduction of controlled systematic variability (CSV) in experimental designs can increase reproducibility. To test this hypothesis, each laboratory followed the same experimental protocol and introduced environmental and genotypic controlled systematic variability (CSV) within and among replicated microcosms established in either growth chambers (with stringent control of environmental conditions) or glasshouses (with more variable environmental conditions). Data were used to test the extent to which the effect size of the net legume effect varied with the CSV treatment and to estimate the number of laboratories that produced results that can be considered reproducible. completed Milcu, Alexandru

alex.milcu@cnrs.fr

https://orcid.org/0000-0002-2889-1234 information principalInvestigator copyright copyright CC-BY-NC-ND-3.0: Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported textTable und geoscientificInformation Laboratory thematicClassification Method comment thematicClassification type of setup Identification thematicClassification Microcosm ID Block thematicClassification Treatment thematicClassification legume Treatment thematicClassification controlled systematic variabiliy Genotype thematicClassification Biomass, dry mass physicalMeasurement seed g g Biomass, dry mass physicalMeasurement shoot g g Biomass, dry mass physicalMeasurement root g g Biomass, dry mass physicalMeasurement total g g Root/shoot ratio thematicClassification biomass Brachypodium distachyon, height physicalMeasurement cm cm Nitrogen, shoot physicalMeasurement % % Carbon, shoot physicalMeasurement % % δ15N physicalMeasurement ‰ air ‰ air δ13C physicalMeasurement ‰ PDB ‰ PDB Evapotranspiration physicalMeasurement ml/day ml/day Litter mass physicalMeasurement mass remaining g g text/tab-separated-values unknown https://doi.pangaea.de/10.1594/PANGAEA.880980?format=textfile download dataset The data set was checked for completeness, correctness, and consistency of metainformation. Validity of used methods was checked and - if applicable - precision and range of data.