Citation:

rsbl.2016.0798

Name: Seawater carbonate chemistry and larvae survival, metabolic rate of oyster Saccostrea glomerata in laboratory experiment
Published: 2017-05-19
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Growth/Morphology; Laboratory experiment; Mollusca; Mortality/Survival; Other; Reproduction; Saccostrea glomerata; Salinity; Single species; South Pacific; Temperature

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Abstract:

Parental effects passed from adults to their offspring have been identified as a source of rapid acclimation that may allow marine populations to persist as our surface oceans continue to decrease in pH. Little is known, however, whether parental effects are beneficial for offspring in the presence of multiple stressors. We exposed adults of the oyster Saccostrea glomerata to elevated CO2 and examined the impacts of elevated CO2 (control = 392; 856 µatm) combined with elevated temperature (control = 24; 28°C), reduced salinity (control = 35; 25) and reduced food concentration (control = full; half diet) on their larvae. Adult exposure to elevated CO2 had a positive impact on larvae reared at elevated CO2 as a sole stressor, which were 8% larger and developed faster at elevated CO2 compared with larvae from adults exposed to ambient CO2 These larvae, however, had significantly reduced survival in all multistressor treatments. This was particularly evident for larvae reared at elevated CO2 combined with elevated temperature or reduced food concentration, with no larvae surviving in some treatment combinations. Larvae from CO2-exposed adults had a higher standard metabolic rate. Our results provide evidence that parental exposure to ocean acidification may be maladaptive when larvae experience multiple stressors.

Keyword(s):

Animalia; Benthic animals; Benthos; Coast and continental shelf; Containers and aquaria (20-1000 L or < 1 m**2); Growth/Morphology; Laboratory experiment; Mollusca; Mortality/Survival; Other; Reproduction; Saccostrea glomerata; Salinity; Single species; South Pacific; Temperature

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Coverage:

Latitude: -31.400000 * Longitude: 152.816670

Date/Time Start: 2015-06-01T00:00:00 * Date/Time End: 2015-06-30T00:00:00

Event(s):

Hastings_river * Latitude: -31.400000 * Longitude: 152.816670 * Date/Time Start: 2015-06-01T00:00:00 * Date/Time End: 2015-06-30T00:00:00 * Method/Device: Experiment (EXP)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-05-17.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Parker, Laura M		study
2	Species	Species		Parker, Laura M
3	Registration number of species	Reg spec no		Parker, Laura M
4	Uniform resource locator/link to reference	URL ref		Parker, Laura M		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Parker, Laura M
6	Treatment	Treat		Parker, Laura M		parents
7	Treatment	Treat		Parker, Laura M		F1 larvae
8	Replicate	Repl		Parker, Laura M
9	Identification	ID		Parker, Laura M
10	Egg size	Egg size	µm	Parker, Laura M		per female
11	Variance	Var		Parker, Laura M		mean egg size per female
12	Egg size, standard error	Egg size std e	±	Parker, Laura M
13	Lipids	Lipids	µg	Parker, Laura M		per egg
14	Shell length	Shell l	mm	Parker, Laura M		day 15, 0=dead
15	Metabolic rate of oxygen per individual	MR O2/ind	ng/#/h	Parker, Laura M		day 15, 0=dead
16	Survival	Survival	%	Parker, Laura M		day 15, 0=dead
17	Larval stages	Larva	%	Parker, Laura M		at the umbonate stage
18	Salinity	Sal		Parker, Laura M		parents
19	Range	Range		Parker, Laura M		salinity, parents
20	Temperature, water	Temp	°C	Parker, Laura M		parents
21	Range	Range		Parker, Laura M		Temperature, water, parents
22	pH	pH		Parker, Laura M		parents, NBS scale
23	Range	Range		Parker, Laura M		pH, NBS scale, parents
24	Alkalinity, total	AT	µmol/kg	Parker, Laura M		parents
25	Range	Range		Parker, Laura M		Alkalinity, total, parents
26	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Parker, Laura M		parents
27	Range	Range		Parker, Laura M		Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), parents
28	Carbon, inorganic, dissolved	DIC	µmol/kg	Parker, Laura M		parents
29	Range	Range		Parker, Laura M		Carbon, inorganic, dissolved, parents
30	Calcite saturation state	Omega Cal		Parker, Laura M		parents
31	Range	Range		Parker, Laura M		Calcite saturation state, parents
32	Aragonite saturation state	Omega Arg		Parker, Laura M		parents
33	Range	Range		Parker, Laura M		Aragonite saturation state, parents
34	Salinity	Sal		Parker, Laura M		F1 larvae
35	Range	Range		Parker, Laura M		salinity, F1 larvae
36	Temperature, water	Temp	°C	Parker, Laura M		F1 larvae
37	Range	Range		Parker, Laura M		Temperature, water, F1 larvae
38	pH	pH		Parker, Laura M		F1 larvae, NBS scale
39	Range	Range		Parker, Laura M		pH, NBS scale, F1 larvae
40	Alkalinity, total	AT	µmol/kg	Parker, Laura M		F1 larvae
41	Range	Range		Parker, Laura M		Alkalinity, total, F1 larvae
42	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Parker, Laura M		F1 larvae
43	Range	Range		Parker, Laura M		Partial pressure of carbon dioxide (water) at sea surface temperature (wet air), F1 larvae
44	Carbon, inorganic, dissolved	DIC	µmol/kg	Parker, Laura M		F1 larvae
45	Range	Range		Parker, Laura M		Carbon, inorganic, dissolved, F1 larvae
46	Calcite saturation state	Omega Cal		Parker, Laura M		F1 larvae
47	Range	Range		Parker, Laura M		Calcite saturation state, F1 larvae
48	Aragonite saturation state	Omega Arg		Parker, Laura M		F1 larvae
49	Range	Range		Parker, Laura M		Aragonite saturation state, F1 larvae
50	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	parents, total scale
52	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	parents
53	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	parents
54	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	parents
55	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	parents
56	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	parents
57	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	parents
58	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	parents
59	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	parents
60	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	F1 larvae, total scale
61	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	F1 larvae
62	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	F1 larvae
63	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	F1 larvae
64	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	F1 larvae
65	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	F1 larvae
66	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	F1 larvae
67	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	F1 larvae
68	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	F1 larvae

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3516 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Treat (parents)	7 Treat (F1 larvae)	8 Repl	9 ID	10 Egg size [µm] (per female)	11 Var (mean egg size per female)	12 Egg size std e [±]	13 Lipids [µg] (per egg)	14 Shell l [mm] (day 15, 0=dead)	15 MR O2/ind [ng/#/h] (day 15, 0=dead)	16 Survival [%] (day 15, 0=dead)	17 Larva [%] (at the umbonate stage)	18 Sal (parents)	19 Range (salinity, parents)	20 Temp [°C] (parents)	21 Range (Temperature, water, parents)	22 pH (parents, NBS scale)	23 Range (pH, NBS scale, parents)	24 AT [µmol/kg] (parents)	25 Range (Alkalinity, total, parents)	26 pCO2water_SST_wet [µatm] (parents)	27 Range (Partial pressure of carbon di...)	28 DIC [µmol/kg] (parents)	29 Range (Carbon, inorganic, dissolved,...)	30 Omega Cal (parents)	31 Range (Calcite saturation state, par...)	32 Omega Arg (parents)	33 Range (Aragonite saturation state, p...)	34 Sal (F1 larvae)	35 Range (salinity, F1 larvae)	36 Temp [°C] (F1 larvae)	37 Range (Temperature, water, F1 larvae)	38 pH (F1 larvae, NBS scale)	39 Range (pH, NBS scale, F1 larvae)	40 AT [µmol/kg] (F1 larvae)	41 Range (Alkalinity, total, F1 larvae)	42 pCO2water_SST_wet [µatm] (F1 larvae)	43 Range (Partial pressure of carbon di...)	44 DIC [µmol/kg] (F1 larvae)	45 Range (Carbon, inorganic, dissolved,...)	46 Omega Cal (F1 larvae)	47 Range (Calcite saturation state, F1 ...)	48 Omega Arg (F1 larvae)	49 Range (Aragonite saturation state, F...)	50 CSC flag	51 pH (parents, total scale)	52 CO2 [µmol/kg] (parents)	53 fCO2water_SST_wet [µatm] (parents)	54 pCO2water_SST_wet [µatm] (parents)	55 [HCO3]- [µmol/kg] (parents)	56 [CO3]2- [µmol/kg] (parents)	57 DIC [µmol/kg] (parents)	58 Omega Arg (parents)	59 Omega Cal (parents)	60 pH (F1 larvae, total scale)	61 CO2 [µmol/kg] (F1 larvae)	62 fCO2water_SST_wet [µatm] (F1 larvae)	63 pCO2water_SST_wet [µatm] (F1 larvae)	64 [HCO3]- [µmol/kg] (F1 larvae)	65 [CO3]2- [µmol/kg] (F1 larvae)	66 DIC [µmol/kg] (F1 larvae)	67 Omega Arg (F1 larvae)	68 Omega Cal (F1 larvae)
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		1	F1	51.03	5.59	0.44						34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		1	F2	48.05	4.22	0.38						34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		1	F3	49.62	1.96	0.26						34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		2	F1	50.03	2.80	0.31						34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		2	F2	50.03	1.96	0.26						34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		2	F3	49.86	1.46	0.22						34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		3	F1	49.83	9.79	0.58						34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		3	F2	50.34	14.16	0.70						34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		3	F3	52.59	13.61	0.69						34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		1	F1	50.17	11.58	0.63						34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		1	F2	49.66	8.81	0.55						34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		1	F3	51.21	15.46	0.73						34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		2	F1	52.41	38.61	1.15						34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		2	F2	46.38	26.60	0.96						34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		2	F3	48.45	10.90	0.61						34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		3	F1	49.28	18.14	0.79						34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		3	F2	50.55	13.83	0.69						34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		3	F3	49.69	7.79	0.52						34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		1					0.00611					34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		2					0.00420					34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Control		3					0.00876					34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08																	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		1					0.00390					34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		2					0.00724					34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	56	Elevated CO2		3					0.00780					34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01																	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	Control	1						0.14200000	0.84	58.99	20	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.2	24	0.5	8.19	0.01	2339	23	392	10	2040	21	5.18	0.08	3.40	0.06	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	8.06	11.52	394.47	395.75	1817.90	212.92	2042.34	3.37	5.14
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2	1						0.12650000	1.48	38.50	10	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.2	24	0.5	7.91	0.02	2339	23	854	27	2185	23	2.99	0.06	1.97	0.04	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+T	1						0.17650000	1.53	0.00	50	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.3	28	0.5	7.92	0.01	2339	23	855	21	2160	28	3.42	0.07	2.27	0.05	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.78	22.30	843.45	846.06	1993.54	141.84	2157.69	2.29	3.45
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+S	1						0.12400000	0.73	41.61	20	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	25.0	0.1	24	0.5	7.82	0.01	1683	11	855	17	1622	12	1.51	0.04	0.96	0.03	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.68	25.86	842.65	845.38	1536.22	58.83	1620.91	0.97	1.53
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+F	1						0.11900000	0.19	33.00	20	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.2	24	0.5	7.91	0.01	2339	23	854	15	2185	19	2.99	0.09	1.97	0.06	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+T+S	1						0.15400000	1.20	26.14	40	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	25.0	0.1	28	0.5	7.83	0.02	1683	11	855	12	1607	9	1.76	0.06	1.14	0.04	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+T+S+F	1						0.14000000	0.62	27.20	30	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	25.0	0.1	28	0.5	7.83	0.02	1683	11	856	14	1607	8	1.76	0.05	1.13	0.03	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	Control	1						0.14525000	2.08	60.33	50	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.2	24	0.5	8.19	0.01	2339	23	392	10	2040	21	5.18	0.08	3.40	0.06	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	8.06	11.52	394.47	395.75	1817.90	212.92	2042.34	3.37	5.14
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2	1						0.13700000	2.39	46.04	20	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.2	24	0.5	7.91	0.02	2339	23	854	27	2185	23	2.99	0.06	1.97	0.04	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+T	1						0.00000000	0.00	0.00	50	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.3	28	0.5	7.92	0.01	2339	23	855	21	2160	28	3.42	0.07	2.27	0.05	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.78	22.30	843.45	846.06	1993.54	141.84	2157.69	2.29	3.45
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+S	1						0.13775000	1.04	38.13	20	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	25.0	0.1	24	0.5	7.82	0.01	1683	11	855	17	1622	12	1.51	0.04	0.96	0.03	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.68	25.86	842.65	845.38	1536.22	58.83	1620.91	0.97	1.53
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+F	1						0.12687500	0.72	5.85	10	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.2	24	0.5	7.91	0.01	2339	23	854	15	2185	19	2.99	0.09	1.97	0.06	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+T+S	1						0.15450000	1.01	12.64	50	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	25.0	0.1	28	0.5	7.83	0.02	1683	11	855	12	1607	9	1.76	0.06	1.14	0.04	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+T+S+F	1						0.00000000	0.00	0.00	60	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	25.0	0.1	28	0.5	7.83	0.02	1683	11	856	14	1607	8	1.76	0.05	1.13	0.03	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	Control	2						0.13625000	0.90	64.04	20	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.2	24	0.5	8.19	0.01	2339	23	392	10	2040	21	5.18	0.08	3.40	0.06	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	8.06	11.52	394.47	395.75	1817.90	212.92	2042.34	3.37	5.14
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2	2						0.12350000	1.62	33.98	20	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.2	24	0.5	7.91	0.02	2339	23	854	27	2185	23	2.99	0.06	1.97	0.04	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+T	2						0.17150000	1.72	7.58	50	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.3	28	0.5	7.92	0.01	2339	23	855	21	2160	28	3.42	0.07	2.27	0.05	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.78	22.30	843.45	846.06	1993.54	141.84	2157.69	2.29	3.45
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+S	2						0.12850000	0.76	60.91	10	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	25.0	0.1	24	0.5	7.82	0.01	1683	11	855	17	1622	12	1.51	0.04	0.96	0.03	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.68	25.86	842.65	845.38	1536.22	58.83	1620.91	0.97	1.53
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+F	2						0.11775000	0.12	30.15	0	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.2	24	0.5	7.91	0.01	2339	23	854	15	2185	19	2.99	0.09	1.97	0.06	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+T+S	2						0.14928570	1.44	17.89	40	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	25.0	0.1	28	0.5	7.83	0.02	1683	11	855	12	1607	9	1.76	0.06	1.14	0.04	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+T+S+F	2						0.13785710	0.63	4.73	40	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	25.0	0.1	28	0.5	7.83	0.02	1683	11	856	14	1607	8	1.76	0.05	1.13	0.03	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	Control	2						0.14950000	1.57	58.46	40	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.2	24	0.5	8.19	0.01	2339	23	392	10	2040	21	5.18	0.08	3.40	0.06	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	8.06	11.52	394.47	395.75	1817.90	212.92	2042.34	3.37	5.14
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2	2						0.13700000	2.10	42.95	20	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.2	24	0.5	7.91	0.02	2339	23	854	27	2185	23	2.99	0.06	1.97	0.04	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+T	2						0.00000000	0.00	0.00	40	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.3	28	0.5	7.92	0.01	2339	23	855	21	2160	28	3.42	0.07	2.27	0.05	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.78	22.30	843.45	846.06	1993.54	141.84	2157.69	2.29	3.45
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+S	2						0.13625000	1.61	36.89	40	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	25.0	0.1	24	0.5	7.82	0.01	1683	11	855	17	1622	12	1.51	0.04	0.96	0.03	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.68	25.86	842.65	845.38	1536.22	58.83	1620.91	0.97	1.53
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+F	2						0.13083300	0.62	24.29	20	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.2	24	0.5	7.91	0.01	2339	23	854	15	2185	19	2.99	0.09	1.97	0.06	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+T+S	2						0.17000000	1.64	4.57	60	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	25.0	0.1	28	0.5	7.83	0.02	1683	11	855	12	1607	9	1.76	0.06	1.14	0.04	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+T+S+F	2						0.00000000	0.00	0.00	70	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	25.0	0.1	28	0.5	7.83	0.02	1683	11	856	14	1607	8	1.76	0.05	1.13	0.03	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	Control	3						0.13625000	0.82	61.45	30	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.2	24	0.5	8.19	0.01	2339	23	392	10	2040	21	5.18	0.08	3.40	0.06	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	8.06	11.52	394.47	395.75	1817.90	212.92	2042.34	3.37	5.14
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2	3						0.12775000	1.71	51.64	0	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.2	24	0.5	7.91	0.02	2339	23	854	27	2185	23	2.99	0.06	1.97	0.04	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+T	3						0.18150000	1.41	2.26	30	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.3	28	0.5	7.92	0.01	2339	23	855	21	2160	28	3.42	0.07	2.27	0.05	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.78	22.30	843.45	846.06	1993.54	141.84	2157.69	2.29	3.45
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+S	3						0.13050000	0.76	44.47	10	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	25.0	0.1	24	0.5	7.82	0.01	1683	11	855	17	1622	12	1.51	0.04	0.96	0.03	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.68	25.86	842.65	845.38	1536.22	58.83	1620.91	0.97	1.53
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+F	3						0.11800000	0.36	22.71	0	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	34.5	0.2	24	0.5	7.91	0.01	2339	23	854	15	2185	19	2.99	0.09	1.97	0.06	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+T+S	3						0.15525000	1.32	6.72	70	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	25.0	0.1	28	0.5	7.83	0.02	1683	11	855	12	1607	9	1.76	0.06	1.14	0.04	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Control	CO2+T+S+F	3						0.13922222	0.66	0.00	30	34.5	0.3	24	0.5	8.20	0.01	2348	18	392	9	2048	25	5.21	0.12	3.42	0.08	25.0	0.1	28	0.5	7.83	0.02	1683	11	856	14	1607	8	1.76	0.05	1.13	0.03	26	8.07	11.24	385.05	386.30	1815.75	217.62	2044.61	3.45	5.25	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	Control	3						0.15650000	1.61	63.80	50	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.2	24	0.5	8.19	0.01	2339	23	392	10	2040	21	5.18	0.08	3.40	0.06	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	8.06	11.52	394.47	395.75	1817.90	212.92	2042.34	3.37	5.14
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2	3						0.14600000	2.01	38.33	20	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.2	24	0.5	7.91	0.02	2339	23	854	27	2185	23	2.99	0.06	1.97	0.04	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+T	3						0.00000000	0.00	0.00	60	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.3	28	0.5	7.92	0.01	2339	23	855	21	2160	28	3.42	0.07	2.27	0.05	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.78	22.30	843.45	846.06	1993.54	141.84	2157.69	2.29	3.45
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+S	3						0.14225000	1.27	53.85	20	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	25.0	0.1	24	0.5	7.82	0.01	1683	11	855	17	1622	12	1.51	0.04	0.96	0.03	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.68	25.86	842.65	845.38	1536.22	58.83	1620.91	0.97	1.53
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+F	3						0.11800000	0.80	7.33	0	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	34.5	0.2	24	0.5	7.91	0.01	2339	23	854	15	2185	19	2.99	0.09	1.97	0.06	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.78	24.56	840.94	843.67	2033.66	124.99	2183.21	1.98	3.02
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+T+S	3						0.16250000	1.55	1.76	70	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	25.0	0.1	28	0.5	7.83	0.02	1683	11	855	12	1607	9	1.76	0.06	1.14	0.04	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78
laboratory	Saccostrea glomerata (mollusk)	397183	marinespecies.org	15	Elevated CO2	CO2+T+S+F	3						0.00000000	0.00	0.00	30	34.5	0.3	24	0.5	7.91	0.01	2348	18	856	21	2194	22	3.01	0.02	1.98	0.01	25.0	0.1	28	0.5	7.83	0.02	1683	11	856	14	1607	8	1.76	0.05	1.13	0.03	26	7.78	24.65	844.28	847.02	2041.68	125.48	2191.82	1.99	3.03	7.68	23.52	847.80	850.42	1515.23	67.49	1606.25	1.14	1.78