Citation:

s11120-016-0330-2

Name: The effects of pH and pCO2 on photosynthesis and respiration in the diatom Thalassiosira weissflogii
Published: 2017-05-08
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Bottles or small containers/Aquaria (<20 L); Chromista; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Thalassiosira weissflogii

Always quote citation above when using data! You can download the citation in several formats below.

RIS Citation BibTeX Citation Text Citation Facebook Twitter

Abstract:

The response of marine phytoplankton to the ongoing increase in atmospheric pCO2 reflects the consequences of both increased CO2 concentration and decreased pH in surface seawater. In the model diatom Thalassiosira weissflogii, we explored the effects of varying pCO2 and pH, independently and in concert, on photosynthesis and respiration by incubating samples in water enriched in H218O. In long-term experiments (6-h) at saturating light intensity, we observed no effects of pH or pCO2 on growth rate, photosynthesis or respiration. This absence of a measurable response reflects the very small change in energy used by the carbon concentrating mechanism (CCM) compared to the energy used in carbon fixation. In short-term experiments (3 min), we also observed no effects of pCO2 or pH, even under limiting light intensity. We surmise that in T. weissflogii, it is the photosynthetic production of NADPH and ATP, rather than the CO2-saturation of Rubisco that controls the rate of photosynthesis at low irradiance. In short-term experiments, we observed a slightly higher respiration rate at low pH at the onset of the dark period, possibly reflecting the energy used for exporting H+ and maintaining pH homeostasis. Based on what is known of the biochemistry of marine phytoplankton, our results are likely generalizable to other diatoms and a number of other eukaryotic species. The direct effects of ocean acidification on growth, photosynthesis and respiration in these organisms should be small over the range of atmospheric pCO2 predicted for the twenty-first century.

Keyword(s):

Bottles or small containers/Aquaria (<20 L); Chromista; Laboratory experiment; Laboratory strains; Light; Not applicable; Ochrophyta; Pelagos; Phytoplankton; Primary production/Photosynthesis; Respiration; Single species; Thalassiosira weissflogii

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-05-12.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Goldman, Johanna A L		study
2	Species	Species		Goldman, Johanna A L
3	Registration number of species	Reg spec no		Goldman, Johanna A L
4	Uniform resource locator/link to reference	URL ref		Goldman, Johanna A L		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Goldman, Johanna A L
6	Experiment	Exp		Goldman, Johanna A L
7	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	ppmv	Goldman, Johanna A L
8	pH	pH		Goldman, Johanna A L		total scale
9	Gross photosynthesis rate, oxygen, per chlorophyll a	PG O2/Chl a	µmol/µg/day	Goldman, Johanna A L
10	Gross photosynthesis rate, standard deviation	PG std dev	±	Goldman, Johanna A L
11	Net photosynthesis rate, oxygen, per chlorophyll a	PN O2/Chl a	µmol/µg/day	Goldman, Johanna A L
12	Net photosynthesis rate, standard deviation	PN std dev	±	Goldman, Johanna A L
13	Time in days	Time	days	Goldman, Johanna A L
14	Cell density	Cells	#/ml	Goldman, Johanna A L
15	Respiration rate, oxygen, per chlorophyll a	Resp O2/Chl a	µmol/µg/day	Goldman, Johanna A L
16	Time in days	Time	days	Goldman, Johanna A L
17	Cell density	Cells	#/ml	Goldman, Johanna A L
18	Replicate	Repl		Goldman, Johanna A L
19	Irradiance	E	µmol/m²/s	Goldman, Johanna A L
20	Gross photosynthesis rate, oxygen, per chlorophyll a	PG O2/Chl a	µmol/µg/day	Goldman, Johanna A L
21	Error	Error		Goldman, Johanna A L		technical error of gross photosynthesis normalized to Chla
22	Net photosynthesis rate, oxygen, per chlorophyll a	PN O2/Chl a	µmol/µg/day	Goldman, Johanna A L
23	Error	Error		Goldman, Johanna A L		technical error of net photosynthesis normalized to Chla
24	Gross photosynthesis rate, oxygen, per cell	PG O2/cell	pmol/#/day	Goldman, Johanna A L
25	Error	Error		Goldman, Johanna A L		technical error of gross photosynthesis normalized to cell numbers
26	Net photosynthesis rate, oxygen, per cell	PN O2/cell	pmol/#/day	Goldman, Johanna A L
27	Error	Error		Goldman, Johanna A L		technical error of net photosynthesis normalized to cell numbers
28	Temperature, water	Temp	°C	Goldman, Johanna A L
29	Salinity	Sal		Goldman, Johanna A L
30	Carbon, inorganic, dissolved	DIC	µmol/l	Goldman, Johanna A L
31	Alkalinity, total	AT	µmol/kg	Goldman, Johanna A L
32	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Alkalinity, total	AT	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
39	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
40	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

3916 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Exp	7 pCO2water_SST_wet [ppmv]	8 pH (total scale)	9 PG O2/Chl a [µmol/µg/day]	10 PG std dev [±]	11 PN O2/Chl a [µmol/µg/day]	12 PN std dev [±]	13 Time [days]	14 Cells [#/ml]	15 Resp O2/Chl a [µmol/µg/day]	16 Time [days]	17 Cells [#/ml]	18 Repl	19 E [µmol/m²/s]	20 PG O2/Chl a [µmol/µg/day]	21 Error (technical error of gross phot...)	22 PN O2/Chl a [µmol/µg/day]	23 Error (technical error of net photos...)	24 PG O2/cell [pmol/#/day]	25 Error (technical error of gross phot...)	26 PN O2/cell [pmol/#/day]	27 Error (technical error of net photos...)	28 Temp [°C]	29 Sal	30 DIC [µmol/l]	31 AT [µmol/kg]	32 CSC flag	33 CO2 [µmol/kg]	34 fCO2water_SST_wet [µatm]	35 [HCO3]- [µmol/kg]	36 [CO3]2- [µmol/kg]	37 DIC [µmol/kg]	38 AT [µmol/kg]	39 Omega Arg	40 Omega Cal
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	constant alkalinity	180	8.4	31.5	1.4	28.7	1.8	0.29	6463														20	33	2271	2822	15	5.70	174.14	1809.01	389.52	2204.24	2734.77	6.12	9.46
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	constant alkalinity	400	8.1	32.7	1.4	29.5	1.3	0.29	6183														20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	constant alkalinity	800	7.8	31.6	3.4	28.8	3.7	0.32	4794														20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	constant pH	180	7.8	26.8	0.1	20.1	0.4	0.24	13288														20	33	500	571	15	5.70	174.14	454.40	24.58	484.68	552.69	0.39	0.60
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	constant pH	400	7.8	27.3	0.6	21.9	0.5	0.25	22308														20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	constant pH	800	7.8	28.1	1.0	22.5	0.2	0.25	20940														20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	varying pH and pCO2	400	8.2	22.3		17.8		0.25	35219	2.8	0.29	34741											20	33	2981	3435	15	12.68	386.98	2536.46	344.60	2893.74	3331.13	5.42	8.37
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	varying pH and pCO2	400	7.8	22.9		17.7		0.25	20515	2.8	0.31	20294											20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	varying pH and pCO2	800	8.2	21.5		17.2		0.25	24968	3.0	0.28	24763											20	33	5962	6757	15	25.35	773.96	5072.91	689.21	5787.48	6556.79	10.83	16.73
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	varying pH and pCO2	800	7.8	19.2		15.7		0.25	40220	2.4	0.28	39921											20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	varying pH and pCO2	800	7.6	22.0		18.1		0.25	24968	2.7	0.31	24337											20	33	1384	1437	15	25.35	773.96	1274.26	43.49	1343.10	1393.57	0.68	1.06
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4	varying pH and pCO2	1360	7.6	21.1		16.7		0.25	20390	2.7	0.27	20294											20	33	2352	2419	15	43.10	1315.73	2166.23	73.93	2283.26	2346.42	1.16	1.79
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	0			-0.81	0.20			-3.08	0.77	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	1	0.29	0.04	-0.43	0.25	1.09	0.14	-1.61	0.95	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	16	0.47	0.03	-0.18	0.12	1.80	0.12	-0.69	0.46	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	28	0.89	0.03	0.35	0.08	3.36	0.11	1.32	0.29	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	47	1.25	0.04	0.81	0.10	4.76	0.13	3.09	0.37	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	68	1.86	0.04	1.43	0.11	7.08	0.16	5.43	0.40	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	100	2.37	0.07	1.88	0.12	8.98	0.25	7.12	0.44	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	145	2.72	0.08	2.53	0.13	10.34	0.29	9.62	0.49	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	227	3.30	0.08	3.42	0.14	12.52	0.30	12.99	0.52	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	330	3.28	0.09	3.91	0.16	12.46	0.36	14.85	0.61	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										A	370	3.17	0.15	3.55	0.27	12.03	0.58	13.49	1.04	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	0			-1.09	0.34			-3.69	1.15	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	3	0.19	0.07	-0.61	0.50	0.66	0.22	-2.08	1.70	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	17	0.62	0.07	0.34	0.20	2.10	0.22	1.14	0.69	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	30	0.97	0.05	0.06	0.03	3.30	0.18	0.20	0.11	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	43	1.19	0.06	0.39	0.11	4.04	0.20	1.31	0.38	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	70	1.69	0.06	1.02	0.14	5.74	0.19	3.47	0.47	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	100	2.18	0.06	1.44	0.14	7.41	0.21	4.90	0.48	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	145	2.69	0.11	2.05	0.16	9.15	0.36	6.94	0.53	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	234	3.11	0.08	2.53	0.15	10.55	0.26	8.61	0.52	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	350	3.27	0.10	2.71	0.16	11.11	0.33	9.21	0.56	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										A	400	3.09	0.11	2.61	0.16	10.50	0.36	8.87	0.55	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	0			-0.88	0.34			-3.29	1.28	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	4	0.36	0.07	-0.47	0.67	1.35	0.25	-1.75	0.75	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	18	0.72	0.05	-0.11	0.20	2.68	0.19	-0.41	0.38	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	35	1.08	0.07	-0.13	0.13	4.02	0.25	-0.47	0.48	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	52	1.44	0.06	1.16	0.15	5.38	0.21	4.32	0.56	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	81	2.12	0.07	1.32	0.16	7.89	0.26	4.93	0.61	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	113	2.90	0.07	2.16	0.17	10.79	0.27	8.05	0.62	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	147	3.06	0.09	2.28	0.17	11.42	0.33	8.49	0.62	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	241	3.42	0.13	2.80	0.21	12.76	0.48	10.46	0.77	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	355	3.44	0.20	3.00	0.24	12.81	0.73	11.17	0.88	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										A	400	3.21	0.12	2.88	0.18	11.95	0.44	10.74	0.68	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	0			-0.85	0.28			-3.86	1.29	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	4	0.16	0.06	-0.62	0.32	0.73	0.29	-2.80	1.45	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	15	0.43	0.04	-0.20	0.20	1.94	0.18	-0.92	0.74	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	27	0.78	0.05	0.22	0.08	3.54	0.22	1.00	0.34	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	35	1.20	0.05	0.92	0.12	5.42	0.21	4.15	0.56	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	60	1.67	0.05	1.24	0.13	7.59	0.24	5.63	0.60	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	95	1.98	0.07	1.69	0.15	8.96	0.30	7.64	0.67	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	143	2.79	0.07	2.58	0.15	12.63	0.30	11.71	0.66	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	193	2.86	0.09	2.45	0.15	12.97	0.39	11.12	0.68	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	320	3.29	0.10	2.91	0.15	14.90	0.44	13.17	0.69	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										A	360	3.28	0.10	2.74	0.14	14.87	0.44	12.41	0.66	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	0			-0.97	0.22			-3.48	0.79	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	5	0.05	0.01	-1.19	0.19	0.16	-0.04	-4.28	0.70	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	16	0.57	0.04	-0.27	0.20	2.05	0.14	-0.98	0.52	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	30	1.01	0.04	0.34	0.09	3.61	0.14	1.22	0.31	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	47	1.29	0.05	0.68	0.11	4.63	0.18	2.46	0.39	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	75	1.84	0.05	1.36	0.13	6.61	0.19	4.87	0.45	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	110	2.22	0.06	1.65	0.13	7.95	0.20	5.90	0.45	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	150	2.74	0.06	2.42	0.13	9.84	0.23	8.69	0.48	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	235	3.32	0.08	2.98	0.14	11.93	0.27	10.70	0.51	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	335	3.49	0.09	3.06	0.15	12.53	0.34	10.97	0.54	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										A	430	3.46	0.11	3.02	0.15	12.40	0.40	10.83	0.55	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	0			-0.74	0.21			-2.84	0.80	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	10	0.14	0.17	-0.86	0.20	0.55	0.63	-3.30	0.75	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	20	0.62	0.05	0.01	0.18	2.36	0.18	0.03	0.68	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	35	0.99	0.05	0.54	0.18	3.78	0.19	2.05	0.71	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	50	1.38	0.06	0.67	0.22	5.28	0.23	2.56	0.83	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	70	1.98	0.06	1.61	0.19	7.56	0.21	6.17	0.71	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	103	2.70	0.07	2.23	0.18	10.35	0.25	8.54	0.69	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	150	3.22	0.09	3.15	0.19	12.33	0.33	12.05	0.72	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	230	3.52	0.10	3.34	0.19	13.46	0.38	12.78	0.75	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	361	3.85	0.15	3.79	0.23	14.73	0.56	14.52	0.88	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	8.1										B	400	4.23	0.12	4.56	0.22	16.20	0.48	17.44	0.86	20	33	2312	2617	15	12.68	386.98	2014.78	217.43	2244.89	2537.77	3.42	5.28
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	0			-0.88	0.20			-3.46	0.80	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	10	0.14	0.19	-0.80	0.19	0.55	0.76	-3.14	0.76	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	20	0.57	0.05	-0.27	0.18	2.25	0.20	-1.06	0.71	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	37	0.90	0.05	0.06	0.17	3.53	0.20	0.23	0.68	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	57	1.32	0.05	0.64	0.17	5.20	0.20	2.53	0.67	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	78	2.02	0.06	1.56	0.18	7.97	0.25	6.15	0.70	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	125	2.74	0.07	2.38	0.17	10.78	0.27	9.37	0.66	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	175	3.23	0.08	3.28	0.17	12.73	0.33	12.92	0.69	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	260	3.78	0.10	3.90	0.18	14.87	0.39	15.38	0.71	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	401	4.42	0.12	4.89	0.20	17.42	0.45	19.27	0.78	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		400	7.8										B	435	3.52	0.15	4.03	0.22	13.88	0.58	15.86	0.85	20	33	1110	1205	15	12.68	386.98	1009.78	54.62	1077.08	1168.14	0.86	1.33
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	0			-0.65	0.18			-2.66	0.74	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	10	0.07	0.05	-0.63	0.18	0.27	0.20	-2.56	0.71	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	18	0.66	0.04	0.23	0.17	2.68	0.15	0.92	0.68	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	31	0.98	0.04	0.64	0.17	3.97	0.17	2.60	0.67	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	49	1.47	0.05	1.10	0.17	5.97	0.19	4.47	0.67	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	80	1.96	0.07	2.05	0.18	7.99	0.28	8.36	0.71	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	114	2.65	0.07	2.65	0.17	10.76	0.27	10.78	0.69	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	150	3.03	0.07	3.07	0.17	12.32	0.28	12.48	0.69	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	250	3.61	0.10	3.66	0.18	14.71	0.39	14.89	0.75	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	370	3.96	0.12	4.84	0.25	16.10	0.48	19.69	1.01	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	8.1										B	400	3.71	0.12	4.05	0.21	15.11	0.47	16.47	0.85	20	33	4625	5140	15	25.35	773.96	4029.56	434.86	4489.77	4987.41	6.83	10.56
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	0			-0.84	0.17			-3.19	0.63	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	5	0.33	0.07	-0.47	0.16	1.25	0.28	-1.81	0.62	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	17	0.64	0.05	-0.14	0.16	2.46	0.17	-0.55	0.60	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	30	0.97	0.04	0.41	0.15	3.70	0.15	1.55	0.57	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	47	1.34	0.05	0.69	0.15	5.12	0.19	2.64	0.58	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	70	1.96	0.06	1.78	0.16	7.45	0.24	6.78	0.61	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	104	2.44	0.06	2.22	0.15	9.30	0.22	8.47	0.57	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	144	2.94	0.08	2.65	0.15	11.19	0.29	10.10	0.58	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	221	3.31	0.08	3.28	0.15	12.61	0.29	12.50	0.58	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	323	3.54	0.10	3.56	0.17	13.49	0.38	13.57	0.65	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		800	7.8										B	400	3.34	0.10	3.68	0.17	12.74	0.37	14.02	0.66	20	33	2220	2358	15	25.35	773.96	2019.56	109.23	2154.15	2287.16	1.72	2.65
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	0			-0.71	0.07			-2.82	0.26	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	6	0.19	0.05	-1.07	2.33	0.73	0.19	-4.22	1.09	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	21	0.58	0.05	-0.64	0.74	2.29	0.20	-2.53	0.98	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	35	1.04	0.05	0.82	0.20	4.12	0.18	3.25	0.77	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	45	1.27	0.05	1.01	0.19	5.01	0.20	3.99	0.76	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	65	2.09	0.07	1.70	0.20	8.25	0.26	6.72	0.80	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	104	2.16	0.09	1.67	0.19	8.54	0.35	6.58	0.73	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	160	2.70	0.10	2.13	0.23	10.65	0.41	8.41	0.90	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	257	3.31	0.09	3.24	0.20	13.06	0.34	12.80	0.79	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	370	3.59	0.14	3.94	0.26	14.17	0.55	15.56	1.03	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		1400	7.8										B	420	3.57	0.11	4.10	0.21	14.09	0.43	16.20	0.84	20	33	3883	4087	15	44.37	1354.43	3534.23	191.16	3769.75	3965.67	3.00	4.64
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	0			-0.58	0.17			-2.12	0.63	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	9	0.19	0.06	-0.33	0.16	0.68	0.23	-1.18	0.60	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	23	0.48	0.04	0.04	0.16	1.76	0.15	0.14	0.58	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	36	0.86	0.04	0.33	0.16	3.11	0.16	1.19	0.58	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	53	1.40	0.05	1.14	0.16	5.08	0.18	4.12	0.57	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	77	1.99	0.05	1.70	0.15	7.23	0.18	6.19	0.56	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	107	2.56	0.06	2.43	0.16	9.28	0.22	8.80	0.57	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	153	3.06	0.08	3.22	0.16	11.12	0.28	11.71	0.59	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	250	3.59	0.09	3.87	0.17	13.03	0.32	14.04	0.63	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	350	3.59	0.11	4.23	0.20	13.06	0.40	15.35	0.73	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38
laboratory	Thalassiosira weissflogii (phytoplankton)	163513	marinespecies.org	4		180	8.1										B	400	3.42	0.12	3.87	0.19	12.41	0.44	14.05	0.69	20	33	1041	1229	15	5.70	174.14	906.65	97.84	1010.20	1190.47	1.54	2.38