Citation:

j.fsi.2017.02.046

Name: Benzo[a]pyrene exposure under future ocean acidification scenarios weakens the immune responses of blood clam, Tegillarca granosa
Published: 2017-05-04
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Animalia; Benthic animals; Benthos; Brackish waters; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Immunology/Self-protection; Laboratory experiment; Mollusca; North Pacific; Organic toxins; Single species; Tegillarca granosa; Temperate

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Abstract:

Persistent organic pollutants (POPs) are known to converge into the ocean and accumulate in the sediment, posing great threats to marine organisms such as the sessile bottom burrowing bivalves. However, the immune toxicity of POPs, such as B[a]P, under future ocean acidification scenarios remains poorly understood to date. Therefore, in the present study, the impacts of B[a]P exposure on the immune responses of a bivalve species, Tegillarca granosa, under present and future ocean acidification scenarios were investigated. Results obtained revealed an increased immune toxicity of B[a]P under future ocean acidification scenarios in terms of reduced THC, altered haemocyte composition, and hampered phagocytosis, which may attribute to the synergetic effects of B[a]P and ocean acidification. In addition, the gene expressions of pathogen pattern recognition receptors (TLR1, TLR2, TLR4, TLR6), pathway mediators (TRAF6, TAK1, TAB2, IKKalpha and Myd88), and effectors (NF-kB) of the important immune related pathways were significantly down-regulated upon exposure to B[a]P under future ocean acidification scenarios. Results of the present study suggested an increased immune toxicity of B[a]P under future ocean acidification scenarios, which will significantly hamper the immune responses of T. granosa and subsequently render individuals more susceptible to pathogens challenges.

Keyword(s):

Animalia; Benthic animals; Benthos; Brackish waters; Containers and aquaria (20-1000 L or < 1 m**2); Gene expression (incl. proteomics); Immunology/Self-protection; Laboratory experiment; Mollusca; North Pacific; Organic toxins; Single species; Tegillarca granosa; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-04-26.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Guangxu		study
2	Species	Species		Liu, Guangxu
3	Registration number of species	Reg spec no		Liu, Guangxu
4	Uniform resource locator/link to reference	URL ref		Liu, Guangxu		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Liu, Guangxu
6	Figure	Fig		Liu, Guangxu
7	Mass	Mass	g	Liu, Guangxu
8	Mass, standard error	Mass std e	±	Liu, Guangxu
9	Tissues	Tissues		Liu, Guangxu
10	Date	Date		Liu, Guangxu
11	Treatment	Treat		Liu, Guangxu
12	Gene expression	Gene expression		Liu, Guangxu		from Toll-like receptor pathway
13	Gene expression, standard error	Gene expression std e	±	Liu, Guangxu		from Toll-like receptor pathway
14	Gene expression	Gene expression		Liu, Guangxu		from NF-kbeta signaling pathway
15	Gene expression, standard error	Gene expression std e	±	Liu, Guangxu		from NF-kbeta signaling pathway
16	pH	pH		Liu, Guangxu	Potentiometric	NBS scale
17	pH, standard deviation	pH std dev	±	Liu, Guangxu	Potentiometric	NBS scale
18	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Liu, Guangxu	Calculated using CO2SYS
19	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Liu, Guangxu	Calculated using CO2SYS
20	Salinity	Sal		Liu, Guangxu
21	Salinity, standard deviation	Sal std dev	±	Liu, Guangxu
22	Temperature, water	Temp	°C	Liu, Guangxu
23	Temperature, water, standard deviation	Temp std dev	±	Liu, Guangxu
24	Alkalinity, total	AT	µmol/kg	Liu, Guangxu	Potentiometric titration
25	Alkalinity, total, standard deviation	AT std dev	±	Liu, Guangxu	Potentiometric titration
26	Carbon, inorganic, dissolved	DIC	µmol/kg	Liu, Guangxu	Calculated using CO2SYS
27	Carbon, inorganic, dissolved, standard error	DIC std e	±	Liu, Guangxu	Calculated using CO2SYS
28	Aragonite saturation state	Omega Arg		Liu, Guangxu	Calculated using CO2SYS
29	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Liu, Guangxu	Calculated using CO2SYS
30	Calcite saturation state	Omega Cal		Liu, Guangxu	Calculated using CO2SYS
31	Calcite saturation state, standard deviation	Omega Cal std dev	±	Liu, Guangxu	Calculated using CO2SYS
32	Hemocyte count	Hemocyte	#	Liu, Guangxu
33	Hemocyte count, standard error	Hemocyte std e	±	Liu, Guangxu
34	Granulocyte, red	Red granulocyte	%	Liu, Guangxu
35	Granulocyte, red, standard error	Red granulocyte std e	±	Liu, Guangxu
36	Granulocyte, basophil	Baso granulocyte	%	Liu, Guangxu
37	Granulocyte, basophil, standard error	Baso granulocyte std e	±	Liu, Guangxu
38	Hyalinocyte	Hyalinocyte	%	Liu, Guangxu
39	Hyalinocyte, standard error	Hyalinocyte std e	±	Liu, Guangxu
40	Phagocytosis	Phago	%	Liu, Guangxu
41	Phagocytosis, standard error	Phago std e	±	Liu, Guangxu
42	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
43	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
44	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
45	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
46	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
47	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
48	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
49	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
50	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
51	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1812 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Fig	7 Mass [g]	8 Mass std e [±]	9 Tissues	10 Date	11 Treat	12 Gene expression (from Toll-like receptor pathway)	13 Gene expression std e [±] (from Toll-like receptor pathway)	14 Gene expression (from NF-kbeta signaling pathway)	15 Gene expression std e [±] (from NF-kbeta signaling pathway)	16 pH (NBS scale, Potentiometric)	17 pH std dev [±] (NBS scale, Potentiometric)	18 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	19 pCO2 std dev [±] (Calculated using CO2SYS)	20 Sal	21 Sal std dev [±]	22 Temp [°C]	23 Temp std dev [±]	24 AT [µmol/kg] (Potentiometric titration)	25 AT std dev [±] (Potentiometric titration)	26 DIC [µmol/kg] (Calculated using CO2SYS)	27 DIC std e [±] (Calculated using CO2SYS)	28 Omega Arg (Calculated using CO2SYS)	29 Omega Arg std dev [±] (Calculated using CO2SYS)	30 Omega Cal (Calculated using CO2SYS)	31 Omega Cal std dev [±] (Calculated using CO2SYS)	32 Hemocyte [#]	33 Hemocyte std e [±]	34 Red granulocyte [%]	35 Red granulocyte std e [±]	36 Baso granulocyte [%]	37 Baso granulocyte std e [±]	38 Hyalinocyte [%]	39 Hyalinocyte std e [±]	40 Phago [%]	41 Phago std e [±]	42 CSC flag (Calculated using seacarb afte...)	43 pH (total scale, Calculated using...)	44 CO2 [µmol/kg] (Calculated using seacarb afte...)	45 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	46 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	47 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	48 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	49 DIC [µmol/kg] (Calculated using seacarb afte...)	50 Omega Arg (Calculated using seacarb afte...)	51 Omega Cal (Calculated using seacarb afte...)
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	control-MyD88	1.02220	0.24868			8.11	0.01	523.68	13.92	20.56	0.15	25.4	0.21	2059.91	11.36	1939.41	12.02	1.66	0.03	2.70	0.05											26	7.96	16.16	533.45	535.15	1776.41	120.82	1913.39	2.03	3.26
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	control-TLR1	1.00890	0.16814			8.11	0.01	523.68	13.92	20.56	0.15	25.4	0.21	2059.91	11.36	1939.41	12.02	1.66	0.03	2.70	0.05											26	7.96	16.16	533.45	535.15	1776.41	120.82	1913.39	2.03	3.26
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	control-TLR2	1.00395	0.10989			8.11	0.01	523.68	13.92	20.56	0.15	25.4	0.21	2059.91	11.36	1939.41	12.02	1.66	0.03	2.70	0.05											26	7.96	16.16	533.45	535.15	1776.41	120.82	1913.39	2.03	3.26
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	control-TLR4	1.00492	0.12104			8.11	0.01	523.68	13.92	20.56	0.15	25.4	0.21	2059.91	11.36	1939.41	12.02	1.66	0.03	2.70	0.05											26	7.96	16.16	533.45	535.15	1776.41	120.82	1913.39	2.03	3.26
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	control-TLR6	1.01078	0.17666			8.11	0.01	523.68	13.92	20.56	0.15	25.4	0.21	2059.91	11.36	1939.41	12.02	1.66	0.03	2.70	0.05											26	7.96	16.16	533.45	535.15	1776.41	120.82	1913.39	2.03	3.26
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	pH7.4 without B[a]P-MyD88	0.43013	0.07582			7.41	0.01	2909.46	25.55	20.58	0.10	25.1	0.16	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01											26	7.26	91.93	3011.61	3021.26	2008.47	27.02	2127.41	0.45	0.73
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	pH7.4 without B[a]P-TLR1	0.47520	0.07878			7.41	0.01	2909.46	25.55	20.58	0.10	25.1	0.16	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01											26	7.26	91.93	3011.61	3021.26	2008.47	27.02	2127.41	0.45	0.73
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	pH7.4 without B[a]P-TLR2	1.77529	0.23123			7.41	0.01	2909.46	25.55	20.58	0.10	25.1	0.16	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01											26	7.26	91.93	3011.61	3021.26	2008.47	27.02	2127.41	0.45	0.73
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	pH7.4 without B[a]P-TLR4	0.45237	0.07122			7.41	0.01	2909.46	25.55	20.58	0.10	25.1	0.16	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01											26	7.26	91.93	3011.61	3021.26	2008.47	27.02	2127.41	0.45	0.73
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	pH7.4 without B[a]P-TLR6	0.54062	0.03992			7.41	0.01	2909.46	25.55	20.58	0.10	25.1	0.16	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01											26	7.26	91.93	3011.61	3021.26	2008.47	27.02	2127.41	0.45	0.73
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	B[a]P-MyD88	0.23959	0.02567			8.11	0.01	522.26	8.16	20.75	0.07	24.2	0.31	2062.31	17.25	1940.60	15.53	1.67	0.04	2.72	0.05											26	7.96	16.49	528.31	530.01	1786.07	117.54	1920.10	1.96	3.15
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	B[a]P-TLR1	0.20351	0.01670			8.11	0.01	522.26	8.16	20.75	0.07	24.2	0.31	2062.31	17.25	1940.60	15.53	1.67	0.04	2.72	0.05											26	7.96	16.49	528.31	530.01	1786.07	117.54	1920.10	1.96	3.15
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	B[a]P-TLR2	0.22184	0.02103			8.11	0.01	522.26	8.16	20.75	0.07	24.2	0.31	2062.31	17.25	1940.60	15.53	1.67	0.04	2.72	0.05											26	7.96	16.49	528.31	530.01	1786.07	117.54	1920.10	1.96	3.15
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	B[a]P-TLR4	0.16090	0.00677			8.11	0.01	522.26	8.16	20.75	0.07	24.2	0.31	2062.31	17.25	1940.60	15.53	1.67	0.04	2.72	0.05											26	7.96	16.49	528.31	530.01	1786.07	117.54	1920.10	1.96	3.15
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	B[a]P-TLR6	0.30583	0.06096			8.11	0.01	522.26	8.16	20.75	0.07	24.2	0.31	2062.31	17.25	1940.60	15.53	1.67	0.04	2.72	0.05											26	7.96	16.49	528.31	530.01	1786.07	117.54	1920.10	1.96	3.15
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	pH7.4 with B[a]P-MyD88	0.02410	0.00314			7.41	0.01	2884.22	38.92	20.70	0.02	25.0	0.22	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01											26	7.26	91.22	2982.57	2992.14	1994.06	26.84	2112.12	0.45	0.72
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	pH7.4 with B[a]P-TLR1	0.10045	0.02305			7.41	0.01	2884.22	38.92	20.70	0.02	25.0	0.22	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01											26	7.26	91.22	2982.57	2992.14	1994.06	26.84	2112.12	0.45	0.72
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	pH7.4 with B[a]P-TLR2	0.20308	0.04635			7.41	0.01	2884.22	38.92	20.70	0.02	25.0	0.22	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01											26	7.26	91.22	2982.57	2992.14	1994.06	26.84	2112.12	0.45	0.72
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	pH7.4 with B[a]P-TLR4	0.07141	0.01245			7.41	0.01	2884.22	38.92	20.70	0.02	25.0	0.22	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01											26	7.26	91.22	2982.57	2992.14	1994.06	26.84	2112.12	0.45	0.72
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	1	8.28	1.85	gills	2016-08-16T00:00	pH7.4 with B[a]P-TLR6	0.06934	0.02392			7.41	0.01	2884.22	38.92	20.70	0.02	25.0	0.22	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01											26	7.26	91.22	2982.57	2992.14	1994.06	26.84	2112.12	0.45	0.72
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	control-TRAF6			1.00270	0.09110	8.11	0.01	523.68	13.92	20.56	0.15	25.4	0.21	2059.91	11.36	1939.41	12.02	1.66	0.03	2.70	0.05											26	7.96	16.16	533.45	535.15	1776.41	120.82	1913.39	2.03	3.26
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	control-TAB2			1.00784	0.15646	8.11	0.01	523.68	13.92	20.56	0.15	25.4	0.21	2059.91	11.36	1939.41	12.02	1.66	0.03	2.70	0.05											26	7.96	16.16	533.45	535.15	1776.41	120.82	1913.39	2.03	3.26
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	control-TAK1			1.00927	0.17261	8.11	0.01	523.68	13.92	20.56	0.15	25.4	0.21	2059.91	11.36	1939.41	12.02	1.66	0.03	2.70	0.05											26	7.96	16.16	533.45	535.15	1776.41	120.82	1913.39	2.03	3.26
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	control-IKKa			0.03278	0.33217	8.11	0.01	523.68	13.92	20.56	0.15	25.4	0.21	2059.91	11.36	1939.41	12.02	1.66	0.03	2.70	0.05											26	7.96	16.16	533.45	535.15	1776.41	120.82	1913.39	2.03	3.26
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	control-NF-kB			0.10037	0.10597	8.11	0.01	523.68	13.92	20.56	0.15	25.4	0.21	2059.91	11.36	1939.41	12.02	1.66	0.03	2.70	0.05											26	7.96	16.16	533.45	535.15	1776.41	120.82	1913.39	2.03	3.26
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	pH7.4 without B[a]P-TRAF6			0.54205	0.14990	7.41	0.01	2909.46	25.55	20.58	0.10	25.1	0.16	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01											26	7.26	91.93	3011.61	3021.26	2008.47	27.02	2127.41	0.45	0.73
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	pH7.4 without B[a]P-TAB2			3.07582	0.57601	7.41	0.01	2909.46	25.55	20.58	0.10	25.1	0.16	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01											26	7.26	91.93	3011.61	3021.26	2008.47	27.02	2127.41	0.45	0.73
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	pH7.4 without B[a]P-TAK1			0.71659	0.07792	7.41	0.01	2909.46	25.55	20.58	0.10	25.1	0.16	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01											26	7.26	91.93	3011.61	3021.26	2008.47	27.02	2127.41	0.45	0.73
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	pH7.4 without B[a]P-IKKa			0.50370	0.04650	7.41	0.01	2909.46	25.55	20.58	0.10	25.1	0.16	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01											26	7.26	91.93	3011.61	3021.26	2008.47	27.02	2127.41	0.45	0.73
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	pH7.4 without B[a]P-NF-kB			0.68777	0.05780	7.41	0.01	2909.46	25.55	20.58	0.10	25.1	0.16	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01											26	7.26	91.93	3011.61	3021.26	2008.47	27.02	2127.41	0.45	0.73
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	B[a]P-TRAF6			0.62876	0.02201	8.11	0.01	522.26	8.16	20.75	0.07	24.2	0.31	2062.31	17.25	1940.60	15.53	1.67	0.04	2.72	0.05											26	7.96	16.49	528.31	530.01	1786.07	117.54	1920.10	1.96	3.15
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	B[a]P-TAB2			1.84344	0.47153	8.11	0.01	522.26	8.16	20.75	0.07	24.2	0.31	2062.31	17.25	1940.60	15.53	1.67	0.04	2.72	0.05											26	7.96	16.49	528.31	530.01	1786.07	117.54	1920.10	1.96	3.15
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	B[a]P-TAK1			0.70078	0.08324	8.11	0.01	522.26	8.16	20.75	0.07	24.2	0.31	2062.31	17.25	1940.60	15.53	1.67	0.04	2.72	0.05											26	7.96	16.49	528.31	530.01	1786.07	117.54	1920.10	1.96	3.15
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	B[a]P-IKKa			0.56899	0.04668	8.11	0.01	522.26	8.16	20.75	0.07	24.2	0.31	2062.31	17.25	1940.60	15.53	1.67	0.04	2.72	0.05											26	7.96	16.49	528.31	530.01	1786.07	117.54	1920.10	1.96	3.15
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	B[a]P-NF-kB			0.64019	0.05198	8.11	0.01	522.26	8.16	20.75	0.07	24.2	0.31	2062.31	17.25	1940.60	15.53	1.67	0.04	2.72	0.05											26	7.96	16.49	528.31	530.01	1786.07	117.54	1920.10	1.96	3.15
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	pH7.4 with B[a]P-TRAF6			0.22182	0.00910	7.41	0.01	2884.22	38.92	20.70	0.02	25.0	0.22	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01											26	7.26	91.22	2982.57	2992.14	1994.06	26.84	2112.12	0.45	0.72
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	pH7.4 with B[a]P-TAB2			1.54510	0.07187	7.41	0.01	2884.22	38.92	20.70	0.02	25.0	0.22	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01											26	7.26	91.22	2982.57	2992.14	1994.06	26.84	2112.12	0.45	0.72
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	pH7.4 with B[a]P-TAK1			0.54109	0.01928	7.41	0.01	2884.22	38.92	20.70	0.02	25.0	0.22	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01											26	7.26	91.22	2982.57	2992.14	1994.06	26.84	2112.12	0.45	0.72
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	pH7.4 with B[a]P-IKKa			0.29325	0.06092	7.41	0.01	2884.22	38.92	20.70	0.02	25.0	0.22	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01											26	7.26	91.22	2982.57	2992.14	1994.06	26.84	2112.12	0.45	0.72
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28	2	8.28	1.85	gills	2016-08-16T00:00	pH7.4 with B[a]P-NF-kB			0.47772	0.07369	7.41	0.01	2884.22	38.92	20.70	0.02	25.0	0.22	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01											26	7.26	91.22	2982.57	2992.14	1994.06	26.84	2112.12	0.45	0.72
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28						pH 8.1 without B[a]P (Control)					8.11	0.01	523.68	13.92	20.56	0.15	23.9	0.20	2059.91	11.36	1939.42	12.02	1.66	0.03	2.70	0.05	87330000	2630000	0.842	0.003	0.127	0.003	0.031	0.002	0.545	0.013	26	7.96	16.64	528.40	530.11	1787.94	115.80	1920.38	1.93	3.11
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28						B[a]P exposure at pH 8.1					8.11	0.01	522.26	8.16	20.75	0.07	24.1	0.10	2062.32	17.25	1940.60	15.53	1.67	0.03	2.72	0.05	72850000	2490000	0.825	0.005	0.150	0.003	0.025	0.003	0.427	0.016	26	7.96	16.53	527.95	529.66	1786.84	117.21	1920.57	1.95	3.14
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28						pH 7.8 without B[a]P					7.80	0.01	1139.20	15.93	20.67	0.07	24.0	0.20	2069.20	17.45	2036.00	16.85	0.87	0.02	1.42	0.03	82890000	8000000	0.829	0.005	0.144	0.006	0.028	0.001	0.537	0.017	26	7.65	36.46	1161.14	1164.91	1924.80	61.47	2022.73	1.02	1.65
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28						B[a]P exposure at pH 7.8					7.80	0.01	1130.02	18.59	20.65	0.02	23.9	0.20	2051.10	13.91	2018.13	13.73	0.86	0.01	1.41	0.02	73240000	2120000	0.756	0.004	0.207	0.004	0.037	0.004	0.433	0.012	26	7.65	36.21	1150.21	1153.94	1908.31	60.71	2005.23	1.01	1.63
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28						pH 7.4 without B[a]P					7.41	0.01	2909.46	25.55	20.58	0.10	23.9	0.20	2071.78	13.83	2142.62	12.73	0.37	0.01	0.60	0.01	52560000	1950000	0.760	0.010	0.212	0.010	0.028	0.001	0.429	0.017	26	7.26	93.79	2977.71	2987.40	2010.78	26.00	2130.57	0.43	0.70
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	28						B[a]P exposure at pH 7.4					7.41	0.01	2884.22	38.93	20.70	0.01	24.0	0.10	2057.07	15.88	2126.96	15.62	0.37	0.01	0.60	0.01	48070000	2070000	0.746	0.005	0.226	0.005	0.029	0.001	0.296	0.008	26	7.26	92.76	2954.64	2964.24	1995.99	25.99	2114.74	0.43	0.70