Dassen, Sigrid; Cortois, Roeland; Martens, H; de Hollander, M; Kowalchuk, George A; van der Putten, Wim H; De Deyn, Gerlinde B (2017): Sequences of the 16S and 18S gene and pH determination of the Jena Experiment main plots measured in 2010 [dataset publication series]. PANGAEA, https://doi.org/10.1594/PANGAEA.874990, Supplement to: Dassen, S et al. (2017): Differential responses of soil bacteria, fungi, archaea and protists to plant species richness and plant functional group identity. Molecular Ecology, 26(15), 4085-4098, https://doi.org/10.1111/mec.14175
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Published: 2017-05-03 • DOI registered: 2017-06-05
Abstract:
In september 2010 bulk soil samples were taken from 82 plots in the Jena Experiment main experiment. Per plot 5 cores of 15 cm depth were taken, pooled and sieved. From the extracted DNA the 16S and 18S rRNA gene was amplified with primer sets 515f/806r and FR 1/FF390. The samples were subjected to Roche 454 automated sequencer and GS FLX system using titanium chemistry (Macrogen Seoul, Korea). The 16S dataset includes bacterial and archaeal sequences, the 18S dataset includes mainly fungal and protist sequences.
Project(s):
The Jena Experiment (JenExp)
Coverage:
Latitude: 50.946100 * Longitude: 11.611300
License:
Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported (CC-BY-NC-SA-3.0)
Size:
2 datasets
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Datasets listed in this publication series
- Dassen, S; Cortois, R; Martens, H et al. (2017): 454-pyrosequencing of the 16S and 18S gene in the Jena Experiment main plots in 2010. https://doi.org/10.1594/PANGAEA.874989
- Dassen, S; Cortois, R; Martens, H et al. (2017): Analysed pH and plot information from Jena Experiment main plots measured in 2010. https://doi.org/10.1594/PANGAEA.874980