Citation:

j.marenvres.2017.01.007

Name: Ocean acidification adversely influences metabolism, extracellular pH and calcification of an economically important marine bivalve, Tegillarca granosa
Published: 2017-05-03
License: https://creativecommons.org/licenses/by/3.0/
Keywords: Acid-base regulation; Animalia; Benthic animals; Benthos; Brackish waters; Calcification/Dissolution; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Mollusca; North Pacific; Other metabolic rates; Respiration; Single species; Tegillarca granosa; Temperate

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Abstract:

Oceanic uptake of CO2 from the atmosphere has significantly reduced surface seawater pH and altered the carbonate chemistry within, leading to global Ocean Acidification (OA). The blood clam, Tegillarca granosa, is an economically and ecologically significant marine bivalve that is widely distributed along the coastal and estuarine areas of Asia. To investigate the physiological responses to OA, blood clams were exposed to ambient and three reduced seawater pH levels (8.1, 7.8, 7.6 and 7.4) for 40 days, respectively. Results obtained suggest that OA suppresses the feeding activity and aerobic metabolism, but elevates proteins catabolism of blood clams. OA also causes extracellular acidosis and decreases haemolymph Ca2+ concentration. In addition, our data also suggest that OA impairs the calcification process and inner shell surface integrity. Overall, OA adversely influences metabolism, acid-base status and calcification of blood clams, subsequently leading to a decrease in the fitness of this marine bivalve species.

Keyword(s):

Acid-base regulation; Animalia; Benthic animals; Benthos; Brackish waters; Calcification/Dissolution; Containers and aquaria (20-1000 L or < 1 m**2); Laboratory experiment; Mollusca; North Pacific; Other metabolic rates; Respiration; Single species; Tegillarca granosa; Temperate

Further details:

Gattuso, Jean-Pierre; Epitalon, Jean-Marie; Lavigne, Héloïse; Orr, James C; Gentili, Bernard; Proye, Aurélien; Soetaert, Karline; Rae, James (2016): seacarb: seawater carbonate chemistry with R. R package version 3.1. https://cran.r-project.org/package=seacarb

Project(s):

Ocean Acidification International Coordination Centre (OA-ICC)

Comment:

In order to allow full comparability with other ocean acidification data sets, the R package seacarb (Gattuso et al, 2016) was used to compute a complete and consistent set of carbonate system variables, as described by Nisumaa et al. (2010). In this dataset the original values were archived in addition with the recalculated parameters (see related PI). The date of carbonate chemistry calculation by seacarb is 2017-04-26.

Parameter(s):

#	Name	Short Name	Unit	Principal Investigator	Method/Device	Comment
1	Type	Type		Liu, Guangxu		study
2	Species	Species		Liu, Guangxu
3	Registration number of species	Reg spec no		Liu, Guangxu
4	Uniform resource locator/link to reference	URL ref		Liu, Guangxu		WoRMS Aphia ID
5	Experiment duration	Exp duration	days	Liu, Guangxu
6	Treatment	Treat		Liu, Guangxu
7	Replicate	Repl		Liu, Guangxu
8	Clearance rate	CR	ml/g/h	Liu, Guangxu
9	Respiration rate, oxygen	Resp O2	mg/kg/h	Liu, Guangxu
10	Ammonia excretion	NH3/[NH4]+ exc	mg/g/h	Liu, Guangxu
11	Oxygen/Nitrogen ratio	O2/N2		Liu, Guangxu
12	Haemolymph, pH	pH (ha)		Liu, Guangxu
13	Haemolymph, calcium ion	Ca2+ (ha)	mg/l	Liu, Guangxu
14	Net calcification rate of calcium carbonate	NC CaCO3	µmol/g/h	Liu, Guangxu
15	pH	pH		Liu, Guangxu	Potentiometric	NBS scale
16	pH, standard deviation	pH std dev	±	Liu, Guangxu	Potentiometric	NBS scale
17	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Liu, Guangxu	Calculated using CO2SYS
18	Partial pressure of carbon dioxide, standard deviation	pCO2 std dev	±	Liu, Guangxu	Calculated using CO2SYS
19	Salinity	Sal		Liu, Guangxu
20	Salinity, standard deviation	Sal std dev	±	Liu, Guangxu
21	Temperature, water	Temp	°C	Liu, Guangxu
22	Temperature, water, standard deviation	Temp std dev	±	Liu, Guangxu
23	Alkalinity, total	AT	µmol/kg	Liu, Guangxu	Potentiometric titration
24	Alkalinity, total, standard deviation	AT std dev	±	Liu, Guangxu	Potentiometric titration
25	Aragonite saturation state	Omega Arg		Liu, Guangxu	Calculated using CO2SYS
26	Aragonite saturation state, standard deviation	Omega Arg std dev	±	Liu, Guangxu	Calculated using CO2SYS
27	Calcite saturation state	Omega Cal		Liu, Guangxu	Calculated using CO2SYS
28	Calcite saturation state, standard deviation	Omega Cal std dev	±	Liu, Guangxu	Calculated using CO2SYS
29	Carbonate system computation flag	CSC flag		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
30	pH	pH		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)	total scale
31	Carbon dioxide	CO2	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
32	Fugacity of carbon dioxide (water) at sea surface temperature (wet air)	fCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
33	Partial pressure of carbon dioxide (water) at sea surface temperature (wet air)	pCO2water_SST_wet	µatm	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
34	Bicarbonate ion	[HCO3]-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
35	Carbonate ion	[CO3]2-	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
36	Carbon, inorganic, dissolved	DIC	µmol/kg	Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
37	Aragonite saturation state	Omega Arg		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)
38	Calcite saturation state	Omega Cal		Yang, Yan	Calculated using seacarb after Nisumaa et al. (2010)

License:

Creative Commons Attribution 3.0 Unported (CC-BY-3.0)

Status:

Curation Level: Enhanced curation (CurationLevelC)

Size:

1216 data points

Data

Download dataset as tab-delimited text — use the following character encoding:

1 Type (study)	2 Species	3 Reg spec no	4 URL ref (WoRMS Aphia ID)	5 Exp duration [days]	6 Treat	7 Repl	8 CR [ml/g/h]	9 Resp O2 [mg/kg/h]	10 NH3/[NH4]+ exc [mg/g/h]	11 O2/N2	12 pH (ha)	13 Ca2+ (ha) [mg/l]	14 NC CaCO3 [µmol/g/h]	15 pH (NBS scale, Potentiometric)	16 pH std dev [±] (NBS scale, Potentiometric)	17 pCO2water_SST_wet [µatm] (Calculated using CO2SYS)	18 pCO2 std dev [±] (Calculated using CO2SYS)	19 Sal	20 Sal std dev [±]	21 Temp [°C]	22 Temp std dev [±]	23 AT [µmol/kg] (Potentiometric titration)	24 AT std dev [±] (Potentiometric titration)	25 Omega Arg (Calculated using CO2SYS)	26 Omega Arg std dev [±] (Calculated using CO2SYS)	27 Omega Cal (Calculated using CO2SYS)	28 Omega Cal std dev [±] (Calculated using CO2SYS)	29 CSC flag (Calculated using seacarb afte...)	30 pH (total scale, Calculated using...)	31 CO2 [µmol/kg] (Calculated using seacarb afte...)	32 fCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	33 pCO2water_SST_wet [µatm] (Calculated using seacarb afte...)	34 [HCO3]- [µmol/kg] (Calculated using seacarb afte...)	35 [CO3]2- [µmol/kg] (Calculated using seacarb afte...)	36 DIC [µmol/kg] (Calculated using seacarb afte...)	37 Omega Arg (Calculated using seacarb afte...)	38 Omega Cal (Calculated using seacarb afte...)
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 8.1	1	669.63	2516.13	0.10306	27.46602	7.49	394.94	1.67987	8.13	0.04	554.03	5.37	21.58	0.22	28.2	0.2	2085.67	19.61	2.25	0.03	3.62	0.04	26	7.97	14.38	511.84	513.41	1753.73	141.15	1909.26	2.42	3.82
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 8.1	2	662.11	2444.44	0.09791	28.08566	7.47	403.33	1.75553	8.13	0.04	554.03	5.37	21.58	0.22	28.2	0.2	2085.67	19.61	2.25	0.03	3.62	0.04	26	7.97	14.38	511.84	513.41	1753.73	141.15	1909.26	2.42	3.82
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 8.1	3	685.39	2590.00	0.09522	30.60049	7.39	387.99	1.69211	8.13	0.04	554.03	5.37	21.58	0.22	28.2	0.2	2085.67	19.61	2.25	0.03	3.62	0.04	26	7.97	14.38	511.84	513.41	1753.73	141.15	1909.26	2.42	3.82
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 8.1	4	657.68	2645.54	0.10656	27.93094	7.48	395.02	1.62177	8.13	0.04	554.03	5.37	21.58	0.22	28.2	0.2	2085.67	19.61	2.25	0.03	3.62	0.04	26	7.97	14.38	511.84	513.41	1753.73	141.15	1909.26	2.42	3.82
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 8.1	5	663.69	2437.50	0.10545	26.00574	7.49	403.27	1.62991	8.13	0.04	554.03	5.37	21.58	0.22	28.2	0.2	2085.67	19.61	2.25	0.03	3.62	0.04	26	7.97	14.38	511.84	513.41	1753.73	141.15	1909.26	2.42	3.82
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 8.1	6	653.13	2598.75	0.08872	32.95450	7.68	387.97	1.73601	8.13	0.04	554.03	5.37	21.58	0.22	28.2	0.2	2085.67	19.61	2.25	0.03	3.62	0.04	26	7.97	14.38	511.84	513.41	1753.73	141.15	1909.26	2.42	3.82
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 8.1	7	675.41	2516.25	0.12697	22.29508	7.54	399.97	1.73413	8.13	0.04	554.03	5.37	21.58	0.22	28.2	0.2	2085.67	19.61	2.25	0.03	3.62	0.04	26	7.97	14.38	511.84	513.41	1753.73	141.15	1909.26	2.42	3.82
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 8.1	8	654.63	2576.00	0.10674	27.14978	7.58	397.64	1.65162	8.13	0.04	554.03	5.37	21.58	0.22	28.2	0.2	2085.67	19.61	2.25	0.03	3.62	0.04	26	7.97	14.38	511.84	513.41	1753.73	141.15	1909.26	2.42	3.82
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.8	1	524.03	2361.21	0.17791	14.93051	7.48	384.88	1.49739	7.80	0.05	1202.05	7.51	21.63	0.47	27.9	0.3	2097.47	21.93	1.34	0.04	1.95	0.02	26	7.65	33.90	1198.14	1201.85	1928.22	72.04	2034.16	1.23	1.95
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.8	2	517.92	2575.00	0.17774	16.29830	7.27	391.66	1.42762	7.80	0.05	1202.05	7.51	21.63	0.47	27.9	0.3	2097.47	21.93	1.34	0.04	1.95	0.02	26	7.65	33.90	1198.14	1201.85	1928.22	72.04	2034.16	1.23	1.95
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.8	3	531.12	2454.35	0.16990	16.25153	7.34	402.32	1.50000	7.80	0.05	1202.05	7.51	21.63	0.47	27.9	0.3	2097.47	21.93	1.34	0.04	1.95	0.02	26	7.65	33.90	1198.14	1201.85	1928.22	72.04	2034.16	1.23	1.95
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.8	4	524.05	2493.75	0.15967	17.57031	7.38	384.81	1.43324	7.80	0.05	1202.05	7.51	21.63	0.47	27.9	0.3	2097.47	21.93	1.34	0.04	1.95	0.02	26	7.65	33.90	1198.14	1201.85	1928.22	72.04	2034.16	1.23	1.95
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.8	5	541.43	2316.67	0.18992	13.72297	7.35	391.39	1.46728	7.80	0.05	1202.05	7.51	21.63	0.47	27.9	0.3	2097.47	21.93	1.34	0.04	1.95	0.02	26	7.65	33.90	1198.14	1201.85	1928.22	72.04	2034.16	1.23	1.95
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.8	6	527.41	2332.76	0.17719	14.81076	7.53	402.39	1.45764	7.80	0.05	1202.05	7.51	21.63	0.47	27.9	0.3	2097.47	21.93	1.34	0.04	1.95	0.02	26	7.65	33.90	1198.14	1201.85	1928.22	72.04	2034.16	1.23	1.95
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.8	7	501.35	2396.05	0.18923	14.24507	7.44	388.29	1.48220	7.80	0.05	1202.05	7.51	21.63	0.47	27.9	0.3	2097.47	21.93	1.34	0.04	1.95	0.02	26	7.65	33.90	1198.14	1201.85	1928.22	72.04	2034.16	1.23	1.95
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.8	8	523.09	2456.25	0.19005	14.53972	7.48	382.27	1.45415	7.80	0.05	1202.05	7.51	21.63	0.47	27.9	0.3	2097.47	21.93	1.34	0.04	1.95	0.02	26	7.65	33.90	1198.14	1201.85	1928.22	72.04	2034.16	1.23	1.95
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.6	1	440.16	2238.89	0.23225	10.84502	7.41	380.55	1.29259	7.62	0.03	1971.04	17.89	21.51	0.43	28.2	0.1	2090.82	20.33	0.85	0.02	1.26	0.02	26	7.47	52.44	1865.95	1871.71	1975.62	49.07	2077.13	0.84	1.33
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.6	2	436.57	2307.50	0.22232	11.67678	7.21	389.78	1.36122	7.62	0.03	1971.04	17.89	21.51	0.43	28.2	0.1	2090.82	20.33	0.85	0.02	1.26	0.02	26	7.47	52.44	1865.95	1871.71	1975.62	49.07	2077.13	0.84	1.33
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.6	3	432.30	2260.71	0.21790	11.67213	7.32	387.65	1.32169	7.62	0.03	1971.04	17.89	21.51	0.43	28.2	0.1	2090.82	20.33	0.85	0.02	1.26	0.02	26	7.47	52.44	1865.95	1871.71	1975.62	49.07	2077.13	0.84	1.33
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.6	4	459.83	2262.90	0.22471	11.32911	7.18	373.59	1.19438	7.62	0.03	1971.04	17.89	21.51	0.43	28.2	0.1	2090.82	20.33	0.85	0.02	1.26	0.02	26	7.47	52.44	1865.95	1871.71	1975.62	49.07	2077.13	0.84	1.33
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.6	5	431.17	2208.87	0.20645	12.03679	7.15	370.35	1.32229	7.62	0.03	1971.04	17.89	21.51	0.43	28.2	0.1	2090.82	20.33	0.85	0.02	1.26	0.02	26	7.47	52.44	1865.95	1871.71	1975.62	49.07	2077.13	0.84	1.33
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.6	6	448.12	2321.77	0.20917	12.48730	7.43	379.27	1.33142	7.62	0.03	1971.04	17.89	21.51	0.43	28.2	0.1	2090.82	20.33	0.85	0.02	1.26	0.02	26	7.47	52.44	1865.95	1871.71	1975.62	49.07	2077.13	0.84	1.33
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.6	7	453.23	2331.03	0.22980	11.41174	7.24	383.19	1.28346	7.62	0.03	1971.04	17.89	21.51	0.43	28.2	0.1	2090.82	20.33	0.85	0.02	1.26	0.02	26	7.47	52.44	1865.95	1871.71	1975.62	49.07	2077.13	0.84	1.33
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.6	8	438.47	2248.68	0.22571	11.20829	7.19	375.43	1.21821	7.62	0.03	1971.04	17.89	21.51	0.43	28.2	0.1	2090.82	20.33	0.85	0.02	1.26	0.02	26	7.47	52.44	1865.95	1871.71	1975.62	49.07	2077.13	0.84	1.33
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.4	1	368.15	2087.50	0.30104	7.80102	7.16	378.98	0.95479	7.39	0.05	3120.11	25.61	21.49	0.25	27.9	0.4	2072.43	25.36	0.53	0.03	0.83	0.03	26	7.24	90.72	3203.99	3213.92	2004.24	29.03	2123.99	0.50	0.79
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.4	2	336.43	2063.89	0.29599	7.84441	7.37	356.65	1.03886	7.39	0.05	3120.11	25.61	21.49	0.25	27.9	0.4	2072.43	25.36	0.53	0.03	0.83	0.03	26	7.24	90.72	3203.99	3213.92	2004.24	29.03	2123.99	0.50	0.79
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.4	3	352.37	2100.00	0.28976	8.15322	7.18	378.78	0.99367	7.39	0.05	3120.11	25.61	21.49	0.25	27.9	0.4	2072.43	25.36	0.53	0.03	0.83	0.03	26	7.24	90.72	3203.99	3213.92	2004.24	29.03	2123.99	0.50	0.79
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.4	4	378.44	2141.67	0.28568	8.43368	7.15	355.98	0.87313	7.39	0.05	3120.11	25.61	21.49	0.25	27.9	0.4	2072.43	25.36	0.53	0.03	0.83	0.03	26	7.24	90.72	3203.99	3213.92	2004.24	29.03	2123.99	0.50	0.79
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.4	5	348.24	2049.19	0.30347	7.59650	7.08	372.72	0.85794	7.39	0.05	3120.11	25.61	21.49	0.25	27.9	0.4	2072.43	25.36	0.53	0.03	0.83	0.03	26	7.24	90.72	3203.99	3213.92	2004.24	29.03	2123.99	0.50	0.79
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.4	6	326.51	2195.65	0.29656	8.32911	7.28	364.53	1.13883	7.39	0.05	3120.11	25.61	21.49	0.25	27.9	0.4	2072.43	25.36	0.53	0.03	0.83	0.03	26	7.24	90.72	3203.99	3213.92	2004.24	29.03	2123.99	0.50	0.79
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.4	7	377.35	2250.00	0.30872	8.19914	7.14	367.75	0.93376	7.39	0.05	3120.11	25.61	21.49	0.25	27.9	0.4	2072.43	25.36	0.53	0.03	0.83	0.03	26	7.24	90.72	3203.99	3213.92	2004.24	29.03	2123.99	0.50	0.79
laboratory	Tegillarca granosa (mollusk)	504471	marinespecies.org	40	pH 7.4	8	383.47	2208.87	0.27011	9.20002	7.23	370.48	0.92586	7.39	0.05	3120.11	25.61	21.49	0.25	27.9	0.4	2072.43	25.36	0.53	0.03	0.83	0.03	26	7.24	90.72	3203.99	3213.92	2004.24	29.03	2123.99	0.50	0.79